Objective To investigate the quality of life and its related factors and countermeasures for nursing home residents in Wuhan, to provide constructive advice for promoting quality of life of residents in nursing home. Methods A convenient sample of 292 nursing home residents from 22 nursing homes was enrolled in Wuhan from September 2012 to August 2013. Several questionnaires were used in this survey including demographic data such as modified version of the quality-of-life concerns in the end of life questionnaire (QOLC-E) and short portable mental status questionnaire (SPMSQ). Results A total of 257 (88%) elderly acquired chronic disease, and the main disorder resulted from heart and lung system (51. 0%), sensory system (43. 2%), musculoskeletal and skin system (37. 0%), and nervous system (32. 9%). The total score of life quality was (3. 02 ± 0. 61), of which discomfort level of body, diet, care and support, negative mood, stress and sense of worth were (3. 49 ± 0. 47), (2. 87 ± 0. 82), (3. 13 ± 0. 53), (3. 13 ± 1.11), (3.16 ±1.14) and (2.33 ±1.36) respectively. The main influencing factors contained chronic disease, social support ( self-feeling of financial burden, feeling of passing into silence, social contact ) , and widowed. Elderly without chronic disease history, with high social support and non-widowed obtained a high score of life quality. Conclusions It is necessary for us to improve the quality of life for nursing home residents in Wuhan. Several factors affect the elderly quality of life, so the faculty of nursing homes, family members as well as the community workers should have responsibility to take appropriate and corresponding measures in many ways to help elderly improve their life quality.

Objective To analyze the epidemic characteristics of pneumoconiosis in Jingmen City from 2011 to 2022, and to provide theoretical basis for formulating the occupational disease prevention plan of the ^“14th Five-Year Plan^” in Jingmen City. Methods The data of pneumoconiosis cases in Jingmen City from 2011 to 2022 were collected from the Occupational Disease and Health Hazard Factors Monitoring Information System, and the information of disease classification, enterprise economic type, scale distribution, regional distribution, job distribution, dust exposure years and so on was statistically analyzed. Results A total of 260 cases of pneumoconiosis were reported in Jingmen City from 2011 to 2022, with an average of 13.81 years of exposure to dust, including 164 cases of coal workers' pneumoconiosis, 90 cases of silicosis and 6 cases of other pneumoconiosis. The cases were mainly distributed in Dongbao District with 160 cases (61.54%), Zhongxiang City with 72 cases (27.69%), and Duodao District with 18 cases (6.92%). The difference was statistically significant（χ²=217.846，P<0.05）. The cases were mainly distributed in private enterprises (76.54%, 199 cases). The enterprise sizes were mainly medium-sized (28.46%, 74 cases) and small (61.15%, 159 cases) enterprises, and the difference was statistically significant (χ²=46.025，P<0.05). Conclusion Coal workers' pneumoconiosis and silicosis are the main types of occupational pneumoconiosis in Jingmen area, which will become the key prevention and control targets in Jingmen City for a long time and should be paid attention to. The cases are mainly concentrated in joint-stock, private economic type enterprises and small and medium-sized enterprises, which should be monitored intensively.

Objective: To compare the carcinogenic abilities of CD133⁺CD44⁺ laryngeal cancer stem cells and general laryngeal cancer stem cells and to identify the mechanism underlying the action of miRNAs. Methods: Solid tumor-derived laryngeal carcinoma stem cells and Hep-2-derived laryngeal carcinoma stem cells were cultured, and CD133⁺CD44⁺ laryngeal cancer stem cells were sorted by flow cytometry. Boden chamber invasion assay, cell migration assay and tumor formation assay were then performed to compare the invasion, migration and tumorigenic abilities of CD133⁺CD44⁺ laryngeal cancer stem cells and general laryngeal cancer stem cells. And then, miRNAs isolated from two laryngeal cancer stem cells were detected and analysed with miRNA chip. Results: (1)In Boyden chamber invasion assay, the cell invasion rate of CD133⁺CD44⁺ laryngeal cancer stem cells was obviously higher (80.2%±2.3% vs. 63.9%±3.2%, t=5.011, P=0.027); (2)CD133⁺CD44⁺ laryngeal cancer stem cells also had higher mobility in cell migration assay (82.9%±1.1% vs. 70.9%±0.6%, t=4.514, P=0.031); (3)In tumor formation assay, the tumor formation rate of CD133⁺CD44⁺ laryngeal cancer stem cells was also higher (80% vs. 50%). What′s more, we identified 15 miRNAs that were significantly upregulated in CD133⁺CD44⁺ laryngeal cancer stem cells and 3 miRNAs that were significantly downregulated in CD133⁺CD44⁺ laryngeal cancer stem cells, compared with normal laryngeal cancer stem cells. Conclusions CD133⁺CD44⁺ laryngeal cancer stem cells have stronger invasion, migration and tumorigenic abilities compared with normal laryngeal cancer stem cells, and the difference of miRNAs′ expression is one of the possible causes.