Objective To test the expression of serine/arginine rich splicing factor 1(SRSF1)and apoptosis inhibiting factor(Survivin) in prostate cancer,and study their correlation with the pathological features of prostate cancer,so as to put forward the new targets in the treatment of prostate cancer. Methods SRSF1 and Survivin protein was determined in 20 prostate tissue samples including prostate cancer(n=12)and benign prostat?ic hyperplasia(n=8)by immunohistochemical SP method. SRSF1 and Survivin was correlated to pathological features,and both the relevance was analyzed(no related reports at home and abroad). Results The positive expression rate of SRSF1 protein in prostate cancer tissue cells was 76.37± 5.06%,which was significantly higher than that of benign prostatic hyperplasia(11.30%±1.09%,P<0.05);the positive expression rate of Survivin protein in prostate cancer tissue cells was 86.93%±3.21%,which was significantly higher than that of benign prostatic hyperplasia(17.67%±1.99%, P<0.05);SRSF1 and Survivin protein expressed in prostate cancer organizations and were positively correlated to pathological Gleason grading, and there was significant correlation(P<0.05). Conclusion SRSF1 and Survivin protein were highly expressed in adenocarcinoma tissue,which were significantly increased than that of benign hyperplasia of prostate tissue. The positive expression SRSF1 and Survivin protein were positively cor?related to pathological Gleason grading.The expression of Survivin protein was elevated with the expression of SRSF1 protein in prostate cancer. These preliminary evidence indicated that SRSF1 may up?regulate the expression of Survivin,and thus promote the occurrence and development of prostate cancer.

Objective: To screen the preliminary phytochemical components, to investigate the acute oral toxicity, the analgesic and anti-inflammatory effects, and to analyze inflammatory factors on mice or rats of 70% ethanol extract of Zygopgyllum macropodum aerial parts (ZME). Methods: Preliminary phytochemical screening was carried out by color reaction. Acute oral toxicity was investigated by body weight, relative organ weight and other toxic signs. Acetic acid induced writhing and hot plate test were used to determine analgesic effect. Acetic acid induced vascular permeability and carrageenan induced paw edema were used to confirm anti-inflammation. Protein in pleural effusion, prostaglandin E2 (PGE2) and tumor necrosis factor alpha (TNF-α) in serum of pleuritic rats induced by carrageenan were analyzed to explore the action mechanisms. The test groups received ZME with 100, 300, 600 mg/kg, the positive control with aspirin (ASP) 200 mg/kg for mice, and ZME with 70, 210, 420 mg/kg and ASP 150 mg/kg for rats orally. The control (C) or negative control (NC) groups received 2% Tween 80 of 10 mL/kg orally. Results: ZME contain flavonoids, saponins, phenols and tannins, steroids, terpenoids, fats and oils, glycosides, carbohydrates, and reducing sugar, but no alkaloids. The lethal dose 50% (LD50) of ZME was greater than 2000 mg/kg and no toxic or deleterious effects and death during 14 d. Oral administration 300 and 600 mg/kg of ZME produced analgesic and anti-inflammatory effects significantly (P < 0.05 or P < 0.001) vs NC. It can reduce the writhing number, prolong the heat resisting time, suppress the permeability of the capillary wall increasing, mitigate the paw edema, reduce the content of protein in pleural effusion, and reduce PGE2 and TNF-α in blood. Conclusions: ZME possessed analgesic and anti-inflammatory activities which related to inhibit the production of protein, PGE2 and TNF-α. The LD50 of ZME treated orally to mice was greater than 2000 mg/kg.

Objective To investigate the relationship of pancreatic β-cell function and insulin resistance with microalbuminuria in a cross -sectional study of patients with type 2 diabetes.Methods A total of 524 partici-pants with type 2 diabetes were recruited in this cross -sectional study.All subjects'height,weight,waist circumfer-ence and blood pressure were measured.Venous blood samples were drawn to measure fasting plasma glucose (FPG), fasting lipids,glycated hemoglobin A1c (HbA1c),fasting C -peptide (FPC).24h -urine was collected to measure urinary albumin excretion rate (UAER).Homeostasis model assessment of pancreatic β-cell function (HOMA -B) and insulin resistance (HOMA -IR)were estimated using fasting plasma C -peptide.According to HOMA -B quar-tile,the subjects were divided into four groups,including q1 -q4.According to HOMA -IR,the subjects were also divided into four groups,including Q1 -Q4.We assessed the crude associations across quartiles of these data with demographic and clinical parameters using a nonparametric test for trend across ordered groups (trend using Stata software).Multivariable logistic regression analysis was performed to assess the relationships of pancreatic β-cell function and insulin resistance with microalbuminuria in patients with type 2 diabetes.Results Trend test showed that UAER gradually reduced with increase of HOMA -B.The UAER values in subjects with q1,q2,q3 and q4 were 8.92(5.53 -28.65),8.55(5.52 -20.95),7.57(4.79 -19.83)and 7.84(5.23 -14.38)μg/min,respectively, and the trend was statistically significant(z =-2.1,P <0.05 ).With HOMA -IR increasing,UAER gradually increased.The UAER values in subjects with Q1,Q2,Q3 and Q4 were 6.73(4.85 -16.52),8.61 (5.2 -20.37), 8.31(4.88 -27.04),8.75(6.03 -25.21)μg/min,respectively,and the trend was also statistically significant(z =2.41,P <0.05).Multivariable logistic regression analysis showed that subjects with the highest quartile of HOMA -B had lower possibility of microalbuminuria than patients with the lowest quartile of HOMA -B (adjusted OR q4 vs. q1 =0.39,95% CI:0.20 -0.76,Wald =7.59,P =0.006).Subjects with the highest quartile of HOMA -IR had higher risk of microalbuminuria than those with the lowest quartile of HOMA -IR (adjusted OR Q4 vs.Q1 =2.00, 95% CI:1.08 -3.72,Wald =4.84,P =0.028).Conclusion Insulin resistance is associated with an increased prevalence of microalbuminuria in type 2 diabetes,while improved pancreatic β-cell function is linked to decreased rates of microalbuminuria for those patients.

Objective To develop nanometer-scale bubbles with surfaces of N-palmitoyl chitosan(PLCS) as ultrasound contrast agent and evaluate its characteristics and acoustic effects in vivo. Methods The PLCS nanobubbles were prepared using a cutting technique at differential high-frequency of shear speed. Both optical and transmission electron micrography were performed to determine the nanobubble size and morphology. Concentration, size-distribution and zeta potential of the PLCS nanobubbles were measured by cell counting chamber, Malvern lazer particle analyzer and zeta-sizer at 1-day, 45-day and 90-day. The acoustic effects of the PLCS nanobubbles on myocardium and renal tissue in 6 normal rats were observed using bolus infusion of the nanobubbles intravenously. The maximum video intensity(VI) was measured.Results The PLCS nanobubbles with nice round-shape and uniform site-distribution were demonstrated.The mean diameter,concentration and zeta potential of the PLCS nanobubbles were (617 ± 12) nm, (7.2 ±0.6) × 109/ml and (52.9 ± 1.3)mV at the 1-day,and all of parameters did not change significantly in 45-day and 90-day ( P ＞ 0. 05). A significant contrast-enhancement was noted on myocardium and renal tissue during infusion of the nanobubbles. VI on both tissues was (15.6 ± 1.1)GU and (27.3 ± 2.5)GU,respectively. The visual contrast-enhancement last up to (10 ± 2)min. Conclusions The PLCS nanometerscale bubbles have excellent physical-features and contrast-enhanced ultrasound effects in vivo. It may develop as a novel contrast ultrasound agent which could cross endothelial cell membrances.

Objective To assess the binding capability of microbubbles targeted to VCAM-1 using the parallel plate flow chamber mimic the pulsatile high-shear flow conditions of artery. Methods Targeted microbubbles were designed by conjugating monoclonal antibodies against mouse VCAM-1 to the lipid shell of the microbubbles via an "avidin-biotin" bridge. The binding and retention of targeted microbubbles to VCAM-1 (MBv) immobilized on a culture dish were assessed in a flow chamber at variable shear stress (0.5～ 16.0 dynes/cm2 ). The pulsatile flow conditions were generated and compared to the continuous flow conditions. The retentive ability of MBv was evaluated by the detachment test. Results The marked binding of MBv were seen in pulsatile and continuous flow conditions at low-shear flow conditions of 0.5 ～ 2dyn/cm2 ,but the binding rate in the pulsatile flow group was higher ( P ＜0. 05) than that in the continuous flow conditions. Furthermore,the marked binding of MBv was still noted at the highest shear rates (4～8dyn/cm2) under pulsatile flow conditions, while it was not observed under continuous flow conditions. The half detachment rate of MBv was high up to (20.7 ± 3. 1)dyn/cm2. Conclusions The targeted microbubbles binding to VCAM-1 specific and effective at high-shear stress under pulsatile flow conditions. The molecular ultrasound imaging can be potentially used in the high-shear conditions artery system.

Adult ; Aged ; Alcohol Drinking ; China ; ethnology ; Fatty Liver, Alcoholic ; etiology ; Female ; Humans ; Male ; Middle Aged ; Surveys and Questionnaires

Objective To investigate the food allergy status of infants and young children in community in Shanghai and the influencing factors.Methods The infants aged 0 ～ 36 months in Huamu community in Shanghai were selected and detected with total total serum IgE and 20 specific IgE.Food elimination tests and food stimulation test was used to confirm the allergy.All the confirmed infants were followed up for 6 months.Results The rate of food allergy in children was 9.82％.The most common allergens were egg whites (39.2％) and milk (27.4％).The most common clinical manifestation was eczema.There was no significant difference in food allergy between infants with different sex and birth ways (P ＞ 0.05).With the decreasing of age,the food allergy rate increased significantly.The rate of food allergy was significantly higher in infants with antibiotics,parental allergies and non breast-feeding infants than that with unused antibiotics,non parental allergies and breast feeding.Logistic factor analysis showed that age,use of antibiotics during pregnancy,breast feeding,and parental allergy were the risk factors for food allergy in infants.Conclusion Age,use of antibiotics during pregnancy,breast-feeding,and parents'allergies can significantly affect food allergies in infants and children.

Objective To investigate the food allergy status of infants and young children in community in Shanghai and the influencing factors.Methods The infants aged 0 ～ 36 months in Huamu community in Shanghai were selected and detected with total total serum IgE and 20 specific IgE.Food elimination tests and food stimulation test was used to confirm the allergy.All the confirmed infants were followed up for 6 months.Results The rate of food allergy in children was 9.82％.The most common allergens were egg whites (39.2％) and milk (27.4％).The most common clinical manifestation was eczema.There was no significant difference in food allergy between infants with different sex and birth ways (P ＞ 0.05).With the decreasing of age,the food allergy rate increased significantly.The rate of food allergy was significantly higher in infants with antibiotics,parental allergies and non breast-feeding infants than that with unused antibiotics,non parental allergies and breast feeding.Logistic factor analysis showed that age,use of antibiotics during pregnancy,breast feeding,and parental allergy were the risk factors for food allergy in infants.Conclusion Age,use of antibiotics during pregnancy,breast-feeding,and parents'allergies can significantly affect food allergies in infants and children.

BACKGROUND:Bibliometrics and visual analyses based on thematic literature are particularly important for understanding the foundation and frontiers of postmenopausal osteoporosis research. OBJECTIVE:To perform bibliometric,citation,and visualization analyses of highly cited SCI papers in postmenopausal osteoporosis research over the last 20 years. METHODS:The top 100 highly cited papers on postmenopausal osteoporosis published between 2003 and 2022 included in SCI-EXPANDED catalog of the Web of Science database were obtained for bibliometric measure and visual analysis using CiteSpace software. RESULTS AND CONCLUSION:The top 100 highly cited papers have a total of 67 377 citations in the Web of Science Core Collection,with an annual average of 49.17 citations per paper.Postmenopausal osteoporosis research primarily involves medical,engineering,biological,and multidisciplinary fields.The subcategories are dominated by endocrinology and metabolism,and medicine:internal medicine.Stable and close cooperative network relationships have been formed globally.United States,University of California System,Cummings,and Steven R are the country,research institution,and author,respectively,with the most highly-cited publications.The frontiers of postmenopausal osteoporosis research mainly include calcium and vitamin D supplementation and fracture risk,clinical studies of bisphosphonates in the treatment of postmenopausal osteoporosis,atypical femur fracture,clinical studies of new drugs and sequential treatment of postmenopausal osteoporosis,predictors of fracture risk,mid-and long-term follow-up of osteoporotic vertebral compression fractures,genetic polymorphisms and hereditary factors,formulation and updating of clinical practice guidelines for postmenopausal osteoporosis.Large cohort studies,high-quality randomized controlled trials,systematic reviews,meta-analyses,and clinical practice guidelines are the great engines that drive the development of clinical research in postmenopausal osteoporosis.We should make efforts in the above areas to improve China's international influence in the field of osteoporosis.

This study assesses the causes and prevention measures of rabies through epidemiological investigation and analysis. A field epidemiological survey was conducted to investigate a case of rabies by fox bite. The onset of symptoms began 50 days after the bite. The patient did not receive standard treatment, rabies vaccination, or rabies immunoglobulin injection. The fox was killed on the spot. Saliva and pre-death blood samples were collected at different periods, and only blood RT-PCR tests yielded positive results. Wild fox bite is a major risk factor of rabies infection in Xinjiang Province, China.
Adult ; Animals ; Brain ; virology ; China ; epidemiology ; Fatal Outcome ; Foxes ; virology ; Humans ; Male ; Rabies ; epidemiology ; transmission ; Zoonoses