Objective To study the expression of retinoblastoma(Rb) protein in patients with breast cancer and its significance.Methods The expressions of Rb protein were detected in 52 patients with breast cancer,20 patients with breast fibroadenoma or breast cystic hyperplasia(control group) by S-P immunohistochemical method.(Results The negative) rate of Rb expression in breast cancer(76.91%) was higher than that in control group(15.00%)(P0.05).Of all 46 follow-up patients with breast cancer,the peritoneal recurrence and metastatic rate was 10.53% in 38 cases of the negative expression of Rb protein,and while the rate was 0 in 8 cases of the positive expression of Rb protein,there was significant difference(P

To explore the effects of artisense IRAK-2 oligonucleotide on the prostacyclin (PGI2) synthesis in human umbilicalvein endothelial cells (HUVEC) induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF).Methods:The HUVECs were transfectedwith antisense interleulkin-1 receptor associated kinase-2 oligonucleotide (IRAK-20DN) and stimulated with IL-1 and TNF. The levels of PGI2release were analyzed by competitive ELISA. Results: Pre-transfection with antisense IRAK-20DN could remarkably decrease the levels ofPGI2 synthesis induced by IL-1 in time- and dose-dependent manner, whereas it could not attenuate the one stimulated by TNF. Conclusion:The response of antisense IRAK-2 ODN to IL-1 and TNF-stimulated PGI2 release were different. IRAK-2 plays a key role in the IL-1 signalingevents leading to PGI2 release.

Malnutrition is common among patients with liver disease, and its severity directly influences the short-term survival. As an important approach for chronic liver disease, nutritional support is especially necessary for long-term rehabilitation and treatment. Many methods (e. g. direct human body measurement, biochemical indicator determination, immunological indicators, nutrition assessment tools, and human composition determination) can be applied for the nutritional status evaluation. Based on the specific disease condition and the patient's tolerance, nutritional supports (with proper nutritional substances and administrative route) should be provided individually.

Objective:To explore the necessity of transitional ICU care for patients with liver cancer after major hepatectomy.Methods:The clinical data of 548 liver cancer patients with major hepatectomy were retrospectively analyzed. According to whether by way of transitional ICU care was used or not, patients were divided into ICU group(92 cases) and none-ICU group(456 cases). The clinical data, postoperative complications and mortality were compared.Results:There were no difference in the incidence of major complications(ascites, liver failure, bile leakage, infection and bleeding), mortality and length of hospital stay between the two groups( P>0.05). But the total hospitalization costs of patients in the ICU group were significantly higher than those in the non-ICU group [(72 019±24 516) yuan vs. (67 002±21 374) yuan, t=2.004, P=0.046]. Multivariate analysis showed that intraoperative blood loss and transfusion were independent risk factors for patients undergoing transitional ICU care. Conclusion:Transitional ICU care does not reduce the incidence of postoperative complications and mortality for major hepatectomy patients, only increase the hospitalization costs.

Cerebral palsy (CP) is a essential disorder result in disability of children. Various treatments, especially rehabilitation, have been used and made some success for CP. This paper reviewed a number of literatures published last decade. The level of evidence and recommendation grade of various treatments was evaluated according to Cochrane.

Objective:To study whether gangliosides inhibit the antigen-presenting capability of epidermal Langerhans cells.Methods:In the vitro test, the purified Langerhans cells were exposed to increasing concentration of gangliosides for 5 hours at 37℃,then KLH was added and incubated for 2 hours at 37℃.At last we added HDK1 and after 72 hours of coculture, levels of IFN-? in culture supernatants were measured by ELISA. In the vivo test, immunity to the S1509a spindle cell carcinoma was induced by s.c. inoculation at weekly intervals into naive syngeneic(CAF1) for a total of three immunizations. Delayed-type hypersensitivity(DTH) was elicited 1 week after the last immunization by injection a hind footpad with TAA-pulsed Langerhans cells incubated with or without gangliosides. Footpad swelling was assessed at 24 and 48 hours with a spring-loaded engineer′s micrometer.Results:The presence of gangliosides during HDK1 activation reduced the expression of IFN-? in vitro test. Gangliosides suppressed Langerhans cells to elicit DTH against TAA in vivo test.Conclusion:Ganglioside inhibit the antigen-presenting capability of epidermal Langerhans cells.

OBJECTIVE:To discuss the contents and importance of clinical pharmaceutical care.METHODS:Our experiences in carrying out clinical pharmaceutical care in our hospital were analyzed through exemplification.RESULTS & CONCLUSION:Clinical pharmaceutical care can help improve the medical quality and reduce medical risks.Pharmacist system should be established in hospital to support the work of clinical pharmacists.

Objective To evaluate the changes of rectal morphology and its clinical value in internal rectal prolapse.Methods The rectal morphology of internal rectal prolapse in thirty-one patients with functional constipation and ten normal control subjects was analysed with defecography.The data were analysed with Mann-Whitney Unonparametric test and Fisher's exact test.Results 23 cases and 2 cases with rectal intussusception in patients group and control group respectively were found.Fisher's exact test was P=0.007.In patients group and control group,the thicknesses of anterior intussusception were(13.51 ±9.42)mm and(3.68±2.34)mm,and posterior intussusception were(5.36±3.92)mm and(2.82±0.99)mm respectively;the intussuscipiens diameters were (37.19±11.79)mm and(25.32±9.25)mm,the intussusceptum lumen diameters were(14.91±4.74)mm and(19.73±6.36)mm,the ratio of intussuscipiens diameters and lumen diameters were 2.82±1.64 and 1.28±0.12,the maximum rectal diameter were(48.97±9.55)mm and(39.84±8.45)mm at rest state.There were significant differences between patients group and control group in above varied values(P＜0.05).Conclusion Defecography can differentiate whole thickness rectal intussusception from rectal mucosal prolapse,which provides the scientific basis for choosing the reasonable treatment.However,caution is required when selecting patients for treatment interventions based on defecography.

To study the mechanisms whereby cisplatin suppresses survival of human esophageal squamous cell carcinoma cells.The cytotoxicity of cisplatin in cisplatin-resistant cell line EC109 /CDDP and its parental cell line EC109 was measured by cell viability assay.Western blotting was used to investigate the protein expression of to-tal p53 and phosphorylated p53 at Ser15.Colony formation assay was employed to evaluate the ability of cells to recover from treatments and form colonies.The results indicated that EC109 /CDDP cells were more resistant to cisplatin-induced cytotoxicity than EC109 cells,with the IC50 values of (20.4 ±4.4)μmol /L and (5.7 ±0.1 )μmol /L,respectively.Although cisplatin did not alter the total protein level of p53,it obviously increased the phosphorylation of p53 at Ser15.Cisplatin inhibited survival of both EC109 /CDDP and EC109.Notably,inhibition of p53 by Pifithrin-αsignificantly promoted recovery of cisplatin-treated EC109 and EC109 /CDDP cells to differ-ent degrees.In this respect,p53 protein was found to be activated in response to cisplatin treatment in both EC109 /CDDP and EC109,which may contribute to the cytotoxic effect of cisplatin.

AIM: To investigate the cytological basis and differentiating conditions of human bone marrow mesenchymal stem cells (hMSCs) differentiated into cells of the endothelial lineage in vitro. METHODS: hMSCs were isolated by density gradient centrifugation and fractionated on a 1 073 g/L Percoll. The combination of VEGF165 and various matrix proteins including fibronectin (FN) and type I collagen (Col) was used to induce hMSCs in vitro. Cells were characterized by immunohistochemistry, cytochemistry, FACS and ultrastructure to identify and detect the differentiated population and markers. RESULTS: hMSCs was positive for KDR. PAS reaction was positive and ultrastructure of hMSCs showed glycogen- pool in ectoplasm. Glycogen reducing or disappear suggested that stem cells have occurred differentiation. Induction of hMSCs resulted in the increase of KDR, β1 integrin and CD34. CONCLUSION: hMSCs were induced to a transit population (TP( )) that differentiated toward the endothelial progenitor cells ( EPC), but not a really EPC. hMSCs pedigree diagram of differentiation was hMSCs→TP→EPC→endothelial cells (Ecs).