Water cultured propagation technology of Polygonum multiflorum was investigated with Rooting Powder No. 2 (ABT 2) comparison experiments, and the dynamic changes of endogenous hormones including indole acetic acid (IAA), abscisic acid (ABA), zeatin riboside (ZRs) contents and activities of indoleacetic acid oxidase (IAAO), polyphenol oxidase (PPO) were analyzed during rooting period. The results showed that rooting percentage of softwood cutting with 50 mg x L(-1) ABT2 and 10 mg x L(-1) ABT2 + 0.2% Urea + 0.2% KH2PO4 treatments was 94%, rooting percentage of softwood cuttings of control was 46% only. The adventitious rooting displayed three distinct phases i. e. root-inducing, root formation and root-elongating phases. The dynamic changes of contents of endogenous plant hormones (IAA, ABA, ZRs) and activities of IAAO, PPO tested were tightly related to the rooting process of soft-wood cuttings in P. multiflorum. During root-inducing phase the contents of IAA, ABA and ZRs decreased sharply, whereas ZRs content and IAAO activity kept higher level. IAA content reached the peak and PPO activity increased obviously during root formation phase, while IAAO activities and ABA, ZRs contents declined to minimum. During root-elongating phase PPO, IAAO activities were higher and IAA, ABA, ZRs contents kept steady. During rooting ABT2 (50 mg x L(-1)) treatment increased the content of IAA and PPO activity in cuttings, while the opposite result occurred in contents of ZRs, ABA and IAAO activity.
Catechol Oxidase ; metabolism ; Culture Techniques ; Kinetics ; Peroxidases ; metabolism ; Plant Growth Regulators ; metabolism ; pharmacology ; Plant Roots ; drug effects ; growth & development ; metabolism ; Polygonum ; drug effects ; growth & development ; metabolism

Green prescription is a written prescription aimed at improving health by promoting physical activity and improving diet， with advantages such as high cost-effectiveness， strong feasibility， and minimal harm to patients. The theory of traditional Chinese medicine （TCM） green prescription integrates the health philosophy of "following rule of yin and yang， and adjusting ways to cultivating health"， the exercise philosophy of balancing yin-yang and the five elements， and the dietary philosophy of moderation and balance， which embody core TCM concepts such as treating disease before its onset and harmony between humans and nature. It has also developed traditional exercise practices like Tai Chi， Baduanjin， Wuqinxi， Yi-Gin-Ching， and Qigong， as well as dietary adjustments like medicated diet and herbal wines. However， it is believed that the TCM green prescription currently suffers from insufficient evidence-based research， low patient awareness and acceptance， and weak basic research. Based on this， it is proposed that large-sample clinical trials should be conducted in the future to improve the quality of evidence-based medicine， basic research can be carried out with the help of artificial intelligence and other methods in research design， the hospital information system （HIS） can be used for control at the implementation level， and publicity and patient education can be strengthened through the new media， so as to promote the development and application of the TCM green prescriptions in the field of global health treatment.