OBJECTIVE To investigate the cause and and prevention experience of lung infection in the aged patients with gastrogavage at home.METHODS Retrospective analysis of lung infection was proceeded in 108 aged patients with gastrogavage at home during three years.RESULTS Among 108 patients with gastrogavage at home 83 patients developed infection in which 69 patients developed lung infection.For the aged patients with gastrogavage at home,inspiration and increasing incidence of retrograde infection from stomach to pharynx and lower respiratory tract were the main causes in developing lung infection.Moreover the lower immunity status,catching cold,changing the stomach tube not on time were the related risk factors in developing lung infection.CONCLUSIONS Enhancing the prevention of respiratory tract infection,increasing the nutrition,changing the stomach tube in time and prevention education would effectively decrease the incidence of infection and enhance the rehabilitation.

0.99).The IL-2 activity of control sample of our laboratory was determined according to recom-binant human IL-2 standard,the ability of IL-2 induced by rat and mouse spleen cells was compared, and the parameters of IL-2 induced by rat spleen cells were detected.The partially purified rat IL-2 was obtained through ammonium sulfate precipitation, gel filtration and chromatography.

MicroRNAs (miRNAs) are a small noncoding family of 22 ut RNAs that play an important role in the negative regulation of gene expression at the post-transcriptional level by base pairing to the 3'untranslated region of target messenger RNAs.miRNAs are involved in a variety of basic processes,such as cell proliferation and apoptosis,metabolism and signaling pathways.A lot of studies have indeed described the aberrant expression of miRNAs in human tumors and have investigated their potential role as oncogenes or tumor suppressor genes,depending on the targets they regulate.Furthermore,more and more studies have demonstrated that an important role of miRNAs in anticancer drug resistance and miRNA expression profiling can correlate with the development of anticancer drug resistance.A systematic and intensive study on the mechanisms of miRNA in anticancer drug resistance will provide us a strong rationale for the development of miRNA-based therapeutic strategies aiming to overcome cancer cell resistance.This paper has reviewed the recent development of miRNAs involved in anticancer drug resistance.

Medical social work is a new arising occupation that provides professional medical service for patients in hospitals and communities based on the development of new medical mode,playing critical role in the therapeutic rehabilitation of certain diseases.Tumor diseases,especially malignant tumors,are serious hazards to human life and health,thus require special medical treatment and therapeutic rehabilitation due to their specific characteristics.Therefore,intervention of medical social work is of irreplaceable significance in the therapeutic rehabilitation of tumor diseases,promoting the realization of rehabilitation,soothing physical pains,providing psychological support,lengthening life expansion,enhancing the living quality of tumor sufferers,and assisting them better to regress to normal social life.

Chimeric antigen receptor (CAR) T cell shows its significant therapeutic effect in part of leukemia and lymphomas, which indicates that transgenic adoptive cellular immunotherapy may be one of the breakthroughs in treatment of solid tumors and blood diseases. The functional inhibition of CAR endogenous cells in the tumor microenvironment , molecular biology function of molecules, difficulties of CAR T cells in clinical application and the solutions , how to improve the clinical efficacy and to reduce toxic side effects for future cancer immunotherapy in clinic are reviewed in this paper.

Based on my training experience in the United States,here introduced the situation of diabetes education and management in the United States,diabetes educator certification and the education program of outpatients and inpatients. This information can provide advice to improve the current status of diabetes education in China.

let-7e is one member of let-7 miRNA family, a class of endogenous noncoding RNA which consists of 22 nucleotides. The recent miRNA profiles investigation and analysis results showed that let-7e could be involved in embryonic stem cells differentiation, tumorigenesis in cancer, temporal lobe epilepsy and sleep loss, lipopolysaccharide induced inflammation by regulating its target mRNA expression. This paper gives a review of the important biological function of let-7e.

Objective To investigate the correlation of Chlamydia trachomatis (Ct) infection with tubal pregnancy by Meta-analysis. Methods Eligible literatures were searched from Chinese websites cnki,wanfangdata and eqvip from 1998 to 2007. Meta-analysis was conducted for the correlation of Ct infection with tubal pregnancy by software RevMan 4.2, and the results were expressed as odds ratio (OR) and 95% confidence intervals (95% CI). Results Thirty-five articles were retrieved, of which 16 were included in the Meta-analysis. The combined ORs of Ct infections in cervix uteri and in uterine tube to tubal pregnancy were7.14 (95% CI: 5.20-9.80) and6.50 (95% CI: 4.52-9.34), respectively. Conclusions There are significant correlation between Chlamydia trachomatis infection and tubal pregnancy.

Objective To analyze the postoperative complications in aged patients receiving pancreaticoduodenectomy. Methods Pancreaticoduodenectomy was employed for the treatment of 52 aged patients. Of the 52 patients with a mean age of 70.5(65-76), 32 were male and 20 female. Postoperative pathological examination confirmed that 24 patients suffered from Vater,s ampullary and duodenal cancers, 16 from pancreatic carcinoma and 12 from cholangiocarcinoma. Results The mortality was 7.7%. Severe complications included bleeding (upper gastrointestinal tract or abdominal cavity),MOF, ARDS, postsurgical gastroparasis syndrome(PGS), mental disorder, pancreaticogastrostomy leak and biliary-enteric anastomotic leak et al. Conclusion MOF is the main lethal complication. Incidence of PGS and mental disorder is high.

OBJECTIVE To establish a method of real-time PCR for detection of mecA gene in meticillin-resistant Staphylococcus aureus(MRSA).METHODS The experiment conditions including Mg2+ concentrations,primers concentrations and probe concentrations were optimized for real-time PCR in the light of factorial design principle.Totally 109 strains of S.aureus were collected and detected respectively in oxacillin disk diffusion method and real-time PCR and MRSA detection rate in the two methods were compared and analyzed.RESULTS Thirty seven MRSA isolates were detected by real-time PCR from the 109 S.aureus strains.The MRSA isolating rate by real-time PCR was conspicuously higher than oxacillin disk diffusion method,by which 27 MRSA isolates were detected.CONCLUSIONS The technology of real-time PCR to rapid identification of MRSA is superior to common PCR and it can make up the deficiency of detecting borderline-resistant strains in oxacillin disk diffusion method.