2.Comparison between curved and unipedicular approach vertebroplasty for treatment of osteoporotic vertebral compression fractures
Rui ZHONG ; Wei JIANG ; Sen XIONG ; Yihao LIU ; Runsheng WANG ; Keya MAO
Chinese Journal of Trauma 2018;34(2):102-108
Objective To evaluate the safety and effectiveness of a curved vertebroplasty (CVP) compared with traditional unipedicular approach vertebroplasty (UVP) in treating osteoporotic vertebral compression fractures (OVCF).Methods This was a retrospective case control study on the clinical data of 77 OVCF patients (12 males,65 females;aged 55-86 years,mean 70.8 years) admitted between July 2013 and December 2016.There were 6 injured vertebrae at T1 10,73 at T11 L2,and 12 at L3 5.The patients were divided into CVP group (36 patients,44 vertebrae) and UVP group (41 patients,47 vertebrae) with no significant difference in baseline clinical variables.Intraoperative and postoperative complications including neurovascular injury were recorded.Operation duration,fluoroscopy frequency,volume of cement per level,cement leakage rate per level treated,cement distribution,and refracture rate were compared between the two groups.Preoperative and postoperative visual analog scale (VAS) and Oswestry disability index (ODI) were compared both within the group and between the groups.Results No severe complications related to puncture were observed.No significant difference was observed for operation duration,fluoroscopy frequency,and cement leakage rate per level treated between the two groups (P > 0.05).Compared with UVP group,CVP group had larger volume of cement per level [(5.0 ± 1.4) ml vs.(4.3 ± 1.6) ml],more uniform cement distribution (none vs.10 cases),and lower refracture rate (0 vs.10%) (P < 0.05).The two groups were followed up for 6-49 months (mean,25.9 months).Significant improvements on the VAS and ODI were noted within each group (P <0.01),but there was no significant difference between the two groups (P > 0.05).Conclusions Both CVP and UVP are safe and effective treatments for OVCF.Compared with UVP,CVP entails more uniform cement distribution and lower refracture rate.
3. Study on autophagy in nucleated red blood cells in patients with myelodysplastic syndromes
Liyan YANG ; Huaquan WANG ; Rong FU ; Wen QU ; Erbao RUAN ; Xiaoming WANG ; Guojin WANG ; Yuhong WU ; Hong LIU ; Jia SONG ; Jing GUAN ; Limin XING ; Lijuan LI ; Huijuan JIANG ; Hui LIU ; Yihao WANG ; Chunyan LIU ; Wei ZHANG ; Zonghong SHAO
Chinese Journal of Hematology 2017;38(5):432-436
Objective:
To investigate the change of autophagy level of bone marrow nucleated red blood cell (RBC) in patients with myelodysplastic syndromes (MDS) .
Methods:
Fifty-four MDS patients and thirty-three controls were enrolled in this study. The mitophagy were observed by transmission electron microscopy (TEM) . The level of autophagy-associated protein LC3B in GlycoA+ nucleated RBC was measured by flow cytometry. The expressions of ULK1 and mTOR mRNA in GlycoA+ nucleated RBC were measured by real-time PCR. The expression of the mitochondrial outer membrane protein TOM20 in GlycoA+ nucleated RBC was detected by Western blot.
Results:
Autophagosomes or autolysosomes were scarcely observed by TEM in MDS patients. The expression of LC3B in GlycoA+ nucleated RBC in high-risk MDS patients (0.22±0.12) was significantly lower than that in normal controls (0.43±0.22,
4. Characteristic and function of peripheral blood mononuclear cells-induced macrophages in patients with myelodysplastic syndrome
Yu HAN ; Huaquan WANG ; Rong FU ; Wen QU ; Erbao RUAN ; Xiaoming WANG ; Guojin WANG ; Yuhong WU ; Hong LIU ; Jia SONG ; Jing GUAN ; Limin XING ; Lijuan LI ; Huijuan JIANG ; Hui LIU ; Yihao WANG ; Chunyan LIU ; Wei ZHANG ; Zonghong SHAO
Chinese Journal of Hematology 2017;38(8):706-709
Objective:
To explore characteristic and function of peripheral blood mononuclear cells (PBMNC) -induced macrophages in patients with myelodysplastic syndrome (MDS) to couple with its progression.
Methods:
A total of 24 MDS patients (11 low-risk patients and 13 high-risk group patients) referred to Department of Hematology of Tianjin Medical University General Hospital and normal controls were enrolled from September 2014 to December 2015. PBMNC was stimulated with GM-CSF to transform to macrophages. The morphology of macrophages was observed by microscope. The quantity of macrophages, CD206 and SIRPα on surface of macrophages were detected by flow cytometry. The phagocytic function of macrophages was analyzed by fluorescence microscopy and flow cytometry.
Results:
The morphology of macrophages from MDS patients was abnormal. The percentage of transformed macrophages was (5.17±3.47) % in patients with MDS, which was lower than that in controls significantly[ (66.18±13.43) %,
5.Expression characteristics of glutamine synthetase of wheat in Escherichia coli.
Mingxin GU ; Yihao WEI ; Xiting JIA ; Shuping XIONG ; Xinming MA ; Xiaochun WANG
Chinese Journal of Biotechnology 2018;34(2):264-274
Glutamine synthetase is a key enzyme in plant nitrogen assimilation. To study the structure of wheat glutamine synthetase isoenzymes, GS1, GSr, GSe, GS2 and GS2p of wheat were cloned into pET-21a, and the expression condition was optimized. Although wheat glutamine synthetase isoenzymes had 70%-80% amino acid sequence homology, the isoforms expressed with different characteristics. Induced at 30 °C, the most expression level of GSr, GSe and GS2 was after 3 h, and of GS1 was at the 7 h whereas no GS2p was expressed, and the GS isoenzymes showed different expression level, with the order of GS1 (22%)>GSr (15%)>GS2 (12%)>GSe (5%). GSe expressed as soluble protein, and GS1 expressed mainly as soluble protein whereas GSr and GS2 expressed as insoluble proteins. Induced at 30 °C for 3 h, mRNA transcript levels of GS isoforms were different, with the order of GSr (7.59)>GS2 (1.84)>GS2p (1.66)>GSe (1.46)>GS1 (1.00). The levels of mRNA transcription were not consistent with the level of the protein translation. The analysis of mRNA secondary structure showed the free energy of translation initiation region of glutamine synthetase isoforms was different, with the order of GS1 (14.4)
6.Application of quantitative proteomics in the study of acute mountain sickness.
Bodan TU ; Xue WEI ; Huiying SHANG ; Zuoxu LIU ; Yihao WANG ; Yue GAO
Chinese Journal of Biotechnology 2023;39(9):3594-3604
Acute mountain sickness (AMS) is a clinical syndrome of multi-system physiological disorder after acute exposure to low pressure and low oxygen at high altitude. Quantitative proteomics can systematically quantify and describe protein composition and dynamic changes. In recent years, quantitative proteomics has been widely used in the prevention, diagnosis, treatment and pathogenesis of many diseases. This review summarizes the progress of quantitative proteomics techniques and its application in the prevention, diagnosis, treatment of AMS and mechanisms of rapidly acclimatizing to plateau, in order to provide a reference for the pathogenesis, early intervention, clinical treatment and proteomic research of AMS.
Humans
;
Altitude Sickness/prevention & control*
;
Proteomics
;
Acute Disease
;
Oxygen/metabolism*
7.Autophagy level of bone marrow mononuclear cells in patients with myelodysplastic syndromes.
Lifang GUO ; Ningbo CUI ; Huaquan WANG ; Rong FU ; Wen QU ; Erbao RUAN ; Xiaoming WANG ; Guojin WANG ; Yuhong WU ; Hong LIU ; Jia SONG ; Jing GUAN ; Limin XING ; Lijuan LI ; Huijuan JIANG ; Hui LIU ; Yihao WANG ; Chunyan LIU ; Wei ZHANG ; Zonghong SHAO
Chinese Journal of Hematology 2015;36(12):1016-1019
OBJECTIVETo investigate the change of autophagy level of bone marrow mononuclear cells(BMMNCs)in patients with myelodysplastic syndromes(MDS).
METHODSThirty- eight patients with MDS and 26 megaloblastic anemia patients were enrolled in this study. The autophagic vacuoles were observed by transmission electron microscopy (TEM) and the quantity of autophagic vacuoles was detected by monodansylcadaverine (MDC) staining. The LC3 protein positive cells were counted by immunofluorescence assays. The expression of Beclin 1, LC3A, mTOR mRNA were measured by real time PCR. The expression of Beclin 1 proteins were detected by Western blotting.
RESULTSThe autophgic vacuoles of double membrane that surrounds lysosomes appeared in MDS patients. The percentage of MDC positive cells was significantly higher in MDS patients[(9.75±2.63)%]than that of controls[(2.90± 0.89)%, P<0.05). The percentage of LC3 protein cells was also increased in MDS patients(6.13±1.03)% vs(1.5±0.58)%, P<0.05). The expression of Beclin 1 and LC3A mRNA in low-risk and intermediate-1 MDS were higher compared with controls (3.61 ± 3.02 vs 1.55 ± 1.03 and 6.56 ± 3.97 vs 1.21 ± 0.95 respectively, both P<0.05). The expression of mTOR mRNA was down- regulated in low- risk and intermediate-1 MDS compared with controls(0.39±0.37 vs 1.50±1.03, P<0.05). There were no significant difference in expression of Beclin 1, LC3 and mTOR mRNA among intermediate-2 and high-risk MDS and controls. Beclin 1 protein expression was higher in low- risk and intermediate- 1 MDS patients(1.257 ± 0.197)than that of controls(0.528±0.086)and inermediate-2 and high-risk MDS patients(0.622±0.118).
CONCLUSIONThe autophagy levels were increased in low- risk and intermediate- 1 MDS, while not enhanced in intermediate-2 MDS. Autophagy might be considered as a cell protective mechanism in MDS. The relatively defective autophagy in intermediate- 2 and high- risk MDS might contribute to disease's progression.
Apoptosis Regulatory Proteins ; metabolism ; Autophagy ; Beclin-1 ; Bone Marrow Cells ; cytology ; Humans ; Membrane Proteins ; metabolism ; Microscopy, Electron, Transmission ; Microtubule-Associated Proteins ; metabolism ; Myelodysplastic Syndromes ; pathology ; TOR Serine-Threonine Kinases ; metabolism ; Vacuoles ; ultrastructure
8.Chinese intracranial hemorrhage imaging database: constructing a structured multimodal intracranial hemorrhage data warehouse.
Yihao CHEN ; Jianbo CHANG ; Qinghua ZHANG ; Zeju YE ; Fengxuan TIAN ; Zhaojian LI ; Kaigu LI ; Jie CHEN ; Wenbin MA ; Junji WEI ; Ming FENG ; Renzhi WANG
Chinese Medical Journal 2023;136(13):1632-1634