Objective: To provide the evidence for the development of new drugs, the modification in chemical structures of the registered drugs and the establishment of the therapeutic treatment involving several drugs. Method:Using cytochrome, drug interac-tion and in vitro investigations as the keywords, CHKD and PubMed were searched, and the corresponding references were summarized from 2003 to 2013. The biological characters of the cytochrome oxidase and the ways on the choice of the suitable enzyme system for the research of drug in vitro pharmacokinetic parameters were analyzed in order to predict the potential drug interactions in vivo. Result:The [I]/Ki value had close relation with the possibility of the potential interactions, however, the calculation of [I] was the difficult point, and the methods should be modified according to the specific situation. Conclusion:The prediction technology of drug metabo-lism and interactions on the basis of in vitro investigations can avoid the in vivo complicated process to the greatest extent, reduce the cycle time and the economic cost for the new drug development, and provide the logical evidence and the risky level for the reasonable application of clinical drugs.

BACKGROUND: Nowadays stem cells have been isolated successfully from the epithelial tissue of human and rodents such as skin, hair follicle, cornea, oral mucous, intestinal mucosa dental epithelial cells, and salivary glands by the Chinese or foreign researchers. But according to the vaginal mucosa stem cells (VMSCs), it is not known in the field of isolation and application.OBJECTIVE: To find out the suitable method of isolation, identification, and culture of human VMSCs.DESIGN: Observational basic study. SETTING: Department of Gynaecology and Obstetrics, the Third Affiliated Hospital of Guangzhou Medical College. MATERIALS: This study was performed in the Zhongshan Ophthalmology Center of Zhongshan Medical College from October 2004 to August 2005. Two-week gestation mice were provided by the Experimental Animal Center of Guangzhou University of Chinese Medicine. The sterile human vaginal mucosa was collected from five patients (25-38 years old) after the plastic operation of vagina from the 3rd Affiliated Hospital of Guangzhou Medical College. METHODS: Vaginal mucosa cells could be harvested from human vagina mucosa treated with trypsin-collagenase digesting. The VMSCs were selected by adhesion to type Ⅳ collagen for 20 minutes. The cell cycles of the VMSCs and primary human vaginal mucosa cells were analyzed by flow cytometry. VMSCs were cultured in vitro with 4 different media, including feeder layer cells plus epidermal total culture media, feeder layer cells plus DMEM/F12 (3:1), epidermal total culture media and DMEM/F12 (3:1). At the 12th day, their colony forming efficiency (CFE), CK19 and CK10 positive rates were compared. Then VMSCs were continuously cultured and passaged till they were apoptosis.MAIN OUTCOME MEASURES: ① Positive rate of CK19 and CK10 and G0/G1 proportion; ② Positive rate of CK19 and CK10, the colony forming efficiency (CFE) and the times be passaged when primary VMSCs were cultured in different media after 12 days. RESULTS: The cells at the G0-G1 phase cell cycle, CK19 and CK10 positive rates between the VMSCs and primary human vaginal mucosa cells were statistical different (P < 0.05). When cultured at 12th day, the VMSCs cultured with feeder layer and epidermal total culture media had the highest CFE and CK19 positive rate and lowest CK10 positive rate. They could be passaged over 15 times in vitro. CONCLUSION: It is an effective way that select VMSCs by adhesion to type Ⅳ collagen for 20 minutes. In this research, culturing VMSCs in vitro with feeder layer and epidermal total culture media was the best way to keep VMSC characteristics.

Objective To investigate the efficacy of sorafenib alone or combination with transarterial chemoembolization(TACE)and percutaneous local cryotherapy(PLCT)for advanced hepatocellular carcinoma patients without operation opportunity. Methods Sixty-four advanced hepatocellular carcinoma patients were selected as our subjects,who were underwent treatment of sorafenib alone or combination with TACE and PLCT. Thirty-two cases with sorafenib therapy were served as sorafenib group and another 32 cases with sorafenib in combination with transarterial chemoembolization and PLCT were served as combination group. All patients were followed up for 6 - 32 months. The treatment efficacy and tumor development were recorded. Results All surgeries of the patients were succeed and no death or serious operation complications occurred. Of 64 patients, 11 were achieved a complete remission( CR),31 cases with partial remission( PR),14 cases with stable development(SD),and 8 cases with progressive disease(PD). In the sorafenib group,3 cases were with CR,11 patients with PR,12 with SD,and 6 patients with PD. In the combination group,8 patients were with CR,20 patients with PR,2 patients with SD and 2 patients with PD,and the difference was significant between the two groups(χ2 = 14. 028,P = 0. 003). The median periods to tumor progression were 20 and 53 weeks in the sorafenib group and the combination group,and the difference was significant( χ2 = 14. 773,P = 0. 000). Conclusion For hepatocellular carcinoma patients without operation opportunity,sorafenib combined with TACE and PLCT can increase the tumor remission rate and prolong the periods to tumor progression in patients with hepatocellular carcinoma.

Objective:To compare the clinical efficacy of cold knife conization and transcervical resection of cervical lesion in the treatment of cervical intraepithelial neoplasia(CIN) Ⅱ,Ⅲ.Methods:The clinical data of 62 patients with CIN Ⅱ,Ⅲ underwent cold knife conization(CKC group) and 114 underwent transcervical resection of cervical lesion(TCRC group)in Zhujiang Hospital Southern Medical University from Jan 1 st 2010 to Jun 1 st 2014 were collected.The patients' age,pregnant frequency,mean operative time,preoperative pathological grading,blood loss during the operation and after the operation,positive resected margin,cervical stenosis and recurrence were compared between the two groups.Results:There was no difference on the patients age,pregnant frequency and preoperative pathological grading between the two groups(P ＞ 0.05).The mean blood loss during the operation (14.16 ± 14.10 ml)and the mean hospital stays(5.73 ± 1.43 days)of CKC group were significantly different from those of TCRC group(29.80 ±20.55 ml,4.75 ±1.23 days) (P＜0.05).There was no statistical difference on the mean operation time,postoperative vaginal bleeding rate,rate of positive resected margin,cervical stenosis rate and recurrence rate between CKC group and TCRC group (33.15 ± 11.42 min vs 33.18 ±14.61 min,33.9％ vs 21.1％,3.2％ vs 5.3％,4.8％ vs 4.4％,4.8％ vs 3.5％,respectively) (P＞0.05).The concordance rate of pre-operation and post operation pathological results of CKC group and TCRC group was 74.2％ (46/62) and 72.8％ (83/114) respectively,both kappa values were ＜ 0.75.Conclusions;Both CKC and TCRCT are safe as well as effective in the treatment of high-grade cervical intraepithelial neoplasia.Compared with CKC,TCRC has the advantage of short hospital stay,while much intraoperative blood loss,which calls for intraoperative attention.

Objective:To investigate the effect of oxaliplatin inhibit the growth in glioma U 87 cells by regulating PI3K/Akt sig-nal pathway.Methods:The glioma U87 cells were cultivated in vitro ,using 0,20,40,80 μg/ml oxaliplatin treated U87 cells 24,36, 48,72 h respectively,MTT was used to detect cell proliferation.Using 40 μg/ml oxaliplatin treated U87 cells 48 h,flow cytometry was used to detect cell cycle and apoptosis .Cells apoptosis protein and PI 3K/Akt pathway protein expression after 40 μg/ml oxaliplatin treated U87 cells 48 h was detected by Western blot .Results:MTT assay showed that compared with the 0μg/ml treatment ,oxaliplatin treatment could significantly inhibited U 87 cell survival ( P<0.01 ) ,40μg/ml oxaliplatin treatment 48 h ,the survival inhibitory was the most obvious.U87 cell cycle was arrested in S phase after 40 μg/ml oxaliplatin treatment 48 h.After 40μg/ml oxaliplatin treatment 48 h,compared with the 0 μg/ml treatment,U87 cell apoptosis rate significantly increased (P<0.01).Western blot showed that after 40μg/ml oxaliplatin treatment , anti-apoptotic factor Bcl-2 expression had significant decreased , pro-apoptotic factors Bax , Cleaved-caspase3 protein expression had significantly increased (P<0.01).PI3K,p-Akt expression was significantly decreased (P<0.01),and Akt expression had no significant change in Akt pathway (P>0.05).Conclusion:Oxaliplatin may suppress U87 cell proliferation,ar-rest cell cycle,and promote apoptosis by inhibit PI3K/Akt signaling pathway.

BACKGROUND:Stem cel transplantation, in recent years, has become a preferred treatment for premature ovarian failure. Umbilical cord blood mononuclear cel s containing a large number of mesenchymal stem cel s, immature stem/progenitor cel s, and endothelial progenitor cel s can be used as an important source of stem cel transplantation.
OBJECTIVE:To study the therapeutic efficacy of human umbilical cord blood mononuclear cel transplantation on radioactive premature ovarian failure in nude mice.
METHODS:120 female BALB/C nude mice were randomly divided into four groups:blank control group without any intervention;model group, intravenous transplantation group, and in situ transplantation group exposed to 60Coγrays, 0.5 Gry per day, for 30 days. After 30 days, premature ovarian failure models were made in the latter three groups. Then, nude mice in the latter three groups were given bilateral ovary injection of 10 μL DMEM, tail vein injection of 10 μL human umbilical cord blood mononuclear cel s (1×1013/L), and bilateral ovary injection of 10 μL human umbilical cord blood mononuclear cel s (1×1013/L), respectively. Thirty days after cel transplantation, serum levels of estradiol, fol icle hormone, luteinizing hormone, inhibin B and vascular endothelial growth factor were detected, cel apoptosis in the ovary tissue and cel survival were observed pathological y.
RESULTS AND CONCLUSION:After modeling, the serum levels of estradiol, inhibin B and vascular endothelial growth factor were significantly reduced (P<0.01), while fol icle hormone and luteinizing hormone levels increased (P<0.01). After transplantation, these indexes were al improved in the in situ transplantation group (P<0.01), and reduced fol icle hormone and luteinizing hormone levels were visible in the intravenous transplantation group (P<0.05, P<0.01). Compared with the model group, in situ transplantation and intravenous injection of human umbilical cord blood mononuclear cel s could effectively reduce cel apoptosis in the ovary tissue (P<0.05), and transplanted cel s were able to survive in the ovary of nude mice. Al these findings show human umbilical cord blood mononuclear cel transplantation do have curative effects on premature ovarian failure in nude mice through the inhibition of apoptosis and the regulation of hormone secretion.

5.5 the separation was not achieved.The compound of cyclodextrin and terbutaline increased with the increase of cyclodextrin when the volume rate of cyclodextrin was 0.4% 1.6%,making terbutaline easier for separation.Conclusion:The types of ? CD,the concentration and pH of buffer are the major factors influencing the separation of terbutaline and it can be completely separated.

Objective To observe the effect of CT-aided scalp surrounding needling on infraction dementia by retaining long time. Methods 53 infraction patients following dementia were randomly divided into treatment group (n=26) and control group (n=27). The control group was treated with CT-aided scalp surrounding needling for 30 minutes, while the treatment group was treated with CT-aided scalp surrounding needling for 3 hours. The course was 8 weeks. The therapeutic effect was assessed by Mini-Mental State Examination(MMSE), Hasegawa Dementia Scale(HDS-R) and Activities of Daily Living (ADL). Results The scores of ADL, MMSE, and HDS-R improved obviously after treatment in two groups (P<0.05), and the treatment group was better than the control group (P<0.05). Conclusion CT-aided scalp surrounding needling by retaining long time may facilitate to enhance the clinical effect on infarction dementia.

The legitimate rights of mental health workers don't get expected protection,which has caused negative influence on the development of mental health work.By introducing the Gantt v.Arnie Sethcase,we an-alyzed the law applicable disputes of doctors who are hurt by the patients with mental disorders,and made legal e-valuation on the views of relevant responsibility identification at home and abroad,and attempted to come up with some suggestions on construction of legitimate interest safeguard mechanism for mental-health workers from the as-pects of legislation and administrative policy.

BACKGROUND: Modern neuroradiological imaging techniques such as CT, MRI, and ultrasound help clinicians idenitify the location and volume of an infarct at present. At present, a widely available and easy laboratory examination for acute cerebral infarction is absent.OBJECTIVE: To investigate the relationship between the content S100-β in serum and infarct volume, and prognosis in patients with acute cerebral infarction.DESIGN: Case-control study.SETTING:Department of Neurology of Shandong Provincial Hospital of Shandong University.PARTICIPANTS: From September 2004 to August 2005, 58 patients with acute ischemic brain infarction less than 24 hours after symptom onset were hospitalized in the Department of Neurology of Shandong Provincial Hospital for evaluation and management and enrolled in case group. With the age of 36-86 years and a mean of (68±14) years. 21 were female and 37 were male. Included criteria: The diagnostic criteria was consistent with that of the Second China Cerebrovascular Disease Conference. Every patient who participated in the study underwent the examination of MRI or CT of the brain on admission, the patients were confirmed to be ones with cerebral infarction. Exclusion criteria: A history of a previous stroke and/or existing disability. 50 healthy participants in the control group were from Health Examination Center, including 32 male and 18 female aged 43-89 years and a mean of (68±9) years. Age means and gender were not significantly different between the case group and the control group (P＞0.05).METHODS:① Venous blood samples (2 mL) were drawn in case group at baseline, 1, 2, 3, 4, 6 and 10 days after symptom onset, and the same agent of samples were drawn in control group only at baseline. Enzymelinked immunosorbent assay was used for S100-β measurement. ② Infarct volume of patient was measured by Simes Somatom sensation cardiac wizard workstation volume for CT on day 7 after symptom onset. Neurological outcome was assessed at 3 months after the onset of symptom with modified Rankin scale (MRS) score.MAIN OUTCOME MEASURES: ① Level of S100-β in serum of the subject in the two groups. ② Final infarct volume of patients in case group on day 7 after symptom onset and functional outcome 3 months after symptom onset.RESULTS: 58 patients and 50 healthy control subjects were enrolled in the study. 6 patients in case group developed complete loss of brain stem reflexes and died within 2 months. The others entered the result analysis.①The level of S100-β protein: The level of S100-β protein increased gradually in the case group, peaked at day 3 [(0.61±0.13) μg/L], and decreased at day 10. The levels of S100-β in 6 days after symptom onset were significantly higher than that in control group. The level of S100-β at day 10 in the case group was similar with the control group. ② The level of serum S100-β content in patients of case group: The serum S100-β content were obviously correlated with the infarct volume at 1, 2, 3, 4, 6days after the symptom onset. S100-β value at day 3 provided the highest correlation coefficients (r=0.937, P ＜ 0.001) ③ The status of the cerebral infarction of patients after 3 months: S100-βmeasures and the MRS scores that were obtained 3 months after cerebral infarction revealed highly significant coefficients ranging by bivariate correlations (r=0.507, P ＜ 0.01).CONCLUSION: The content S100-β in serum and infarct volume of the patients with acute cerebral infarction revealed positive correlation. The content S100-β in serum can help to calculate neurological outcome of patients after acute cerebral infarction.