Objective To investigate the preparation of internal quality control products of NT‐proBNP by electrochemilumines‐cence detection .Methods The serum with high and normal levels of NT‐proBNP were collected and divided into low‐value group and high ‐value group .The expected target value ,inter‐batch duplicability ,imprecision and the stability within batch were detected . After effective verification ,we evaluated the bias between self developed internal quality control products and Roche factory quality control materials by comparative test .Results The repeatability ,stability and imprecision of self developed NT‐proBNP were all appropriate to internal quality control products and meeting meet the clinical testing standards .The control range was significantly less than that permitted by Roche ,so it can meet the internal quality control requirements .Conclusion The self developed NT‐proBNP internal quality control products can replace the imports quality control products .

Objective To evaluate the efficacy of minimally invasive technique by retroperitoneal approach in the treatment of severe acute pancreatitis with peripancreatic necrosis.Methods The clinical data of 10 patients with severe acute pancreatitis combined with peripancreatic necrosis who underwent retroperitoneal drainage by aspirating and placing tube under B ultrasound guidance on the basis of routine therapy in the First Affiliated Hospital of University of South China from January 2016 to September 2016 were analyzed.Results All of the 10 patients successfully underwent puncture.Drainage was good after puncture and tube placement in 7 patients.The amylase activity was normal within 3 ～ 7 days,and the symptoms of abdominal pain and bloating were alleviated in 2 ～ 4 days.Among them,3 cases had poor drainage after catheterization due to a large necrotic area,and the percutaneous nephroscope was performed to remove the necrotic tissue of the pancreas.Bacteria was detected in the drainage fluid of 7 patients.The complications of pneumothorax,hemothorax and organ injury were not found in all the patients.The average hospitalization time was 19.4 days (10 ～ 34 days).Conclusions Minimally invasive retroperitoneal approach is safe and effective in curing severe acute pancreatitis with peripancreatic necrosis with good efficacy,which is worth for clinical recommendation and application.

Objective To explore a brief regimen to treat and manage asthmatic patients.Methods 122 asthmatic patients(including 12 inpatients) treated and managed at respiratory clinic were followed up for 1～10 years.The data of these patients were collected and analysed Results On the base of asthma education patients were received inhaled glucocorticoid combined with ? 2 agonist and/or theophylline for two to five years Of 122 patients,20(16 4%) patients remised and remained good control without any medication,90(73 8%) patients relapsed within 1 to 2 years after drug withdrawal Conclusions Asthmatic patients must be treated and managed regularly according to GINA It was especially emphasized that inhaled glucocorticoid is the mainstay of ling term threapy Most patients can remise for quite a long time with agreeable quality of life while a few patients can be cured

Objective To investigate the therapeutic efficacy of Aegisy retrievable filter in inferior vena cava interventional treatment for acute deep venous thrombosis of lower extremity. Methods Acute or sub acute unilateral lower extremity deep vein thrombosis ( deep vein thrombosis, DVT) patients in our hospital were selected, and Aegisy filter was put into the inferior vena cava through the femoral, and thrombolytic therapy was carried out in the next day. Limb conditions was observation after 10 to 15 days, confirmed by vascular ultrasound and angiography of lower limb vein after no fresh or free thrombus. The filter was removed with protector, anticoagulant therapy after review the venography of lower limb postoperative. Results All Aegisy filter were put into patients, and there were no obvious forward jumping and drawing back occurred during the release of the filters, and all Aegisy filter had a better position. Placement of a filter everage time was (16. 8 ± 1. 3) days, 89 cases were successfully recovery filter. The effct of Aegisy filter on the patients with lower extremity deep venous thrombosis after treatment is obvious effect, no pain, and no symptom of pulmonary embolism. Conclusion Aegisy retrievable inferior vena cava filter has many advantages in clinical use. The filter can be placed to the scheduled position accurately,and it is very powerful in captu-ring the thrombus,it can be hold in the inferior vona cava for long time before it is planed to be retrieved,and its retrieval success is very high. Moreover,with high technieal successful rate the implantation of Aegisy retrievable inferior vena cava filter is very safe and effective for the interventional treatment of acute deep venous thrombosis of lower extremity.

Objective To explore the feasibility of lithium heparin anticoagulant plasma instead of serum in biochemical test . Methods 54 biochemical indexes were comparatively detected in 100 samples of lithium heparin anticoagulant plasma and serum . Results The detection results of 45 biochemical indexes in 100 samples of lithium heparin anticoagulant blood plasma and serum showed no statistically significant differences (P>0 .05) .There were statistically significant differences in the indexes of total pro-tein(TP) ,potassium ion(K+ ) ,lactic dehydrogenase(LDH) ,glucose(GLU) ,creatine kinase-MB(CK-MB) and lipase(LPS) between lithium heparin anticoagulant plasma and serum(P<0 .05) ,while results showed good correlation(the maximum r value was 0 .998 , the minimum r value was 0 .887);3 indexes of transferrin(TRF) ,α-L-fucosidase(AFU) and leucine aminopeptidase (LAP) had statistically significant differences (P<0 .05) and showed no correlation(r<0 .6) .Conclusion Lithium heparin has the strongly an-ticoagulant ability with the advantages of non-influence on cell volume and no hemolysis ,which can be used for routine biochemical test ,especially suitable for the outpatient service ,emergency and the patients with blood coagulation dysfunction ,but the detection of TRF ,AFU and LAP can not be suitable .

Objective To explore the clinicopathological features,immunohistochemical characteristics of gastrointestinal anaplastic large cell lymphoma (ALCL).Methods Clinical manifestations,histology and immunophenotype of three cases diagnosed with gastrointestinal ALCL were analyzed with the review of related literatures.Results The clinical manifestations of gastrointestinal ALCL were of no characteristical changes.The pathological features were pleomorphic in which some bizarre,horseshoed or kidney-shaped nuclei were seen.The characteristic of one of the three cases was shown as sarcomatoid.All cases expressed LCA,CD3,CD4,CDs,CD45Ro,CD30,two out of three cases expressed ALK protein,otherwise they were negative for B cell related antigens,CD15 and muscle,nerve,epithelium originated antigens.Conclusions ALCL of the gastrointestinal tract is extremely rare as one of the lymphatic hematopoietic system tumors.No definite clinical characteristic is seen in the gastrointestinal ALCL with high grade malignancy.It is easily misdiagnosed as carcinomas or other sarcomas.It is necessary to use biopsy to make a pathological diagnosis.

ObjectiveTo investigate the clinical efficacy of coiling-dragon warm needling atHuatuo jiaji points plus Chinese herbal fumigation in treating ankylosing spondylitis (AS).MethodEighty AS patients were randomly allocated to treatment and control groups, 40 cases each. The treatment group received coiling-dragon warm needling at Huatuo jiaji points plus Chinese herbal fumigation and the control group, oral administration of salicylazosulfapyridine (SASP). An investigation was made of the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), the patients’ overall assessment, the Bath Ankylosing Spondylitis Functional Index (BASFI), the total number of swollen joints, the Bath Ankylosing Spondylitis Measurement Index (BASMI) and laboratory inflammatory indices: erythrocyte sedimentation rate (ESR), platelet (PLT) and C reactive protein (CRP).ResultThere was a statistically significant difference in the marked efficacy rate between the two groups (P<0.05). Clinical indices: disease activity index and functional index and laboratory indices: ESR, PLT and CRP improved somewhat inboth groups of patients after treatment (P<0.05). After two courses of treatment, the therapeutic effect was better in the treatment group and there was a statistically significant difference compared with the control group (P<0.05).ConclusionCoiling-dragon warm needling at Huatuo jiaji points plus Chinese herbal fumigation can effectively relieve the clinical symptoms and reduce inflammatory reactions in the active stage in AS patients. It is an effective way to treat AS.

0.05).Conclusions The local cell-mediated immunity of PTC is in the status of obviously suppressed.But the local impaired immune response against carcinoma is not associated with lymph node metastasis of PTC.

Objective To study the therapeutic effect of Fe3O4 nanometer magnetic fluid-induced hyperthermia on implanted liver cancer in nude mice under alternating magnetic field. Methods Nude mice model bearing implanted HepG2 was established. Mice were then randomly divided into 3 groups: the blank control group; the magnetic field group; nanometer magnetic fluid group. The magnetic field group were just put under the magnetic field; Nanometer magnetic fluid group received injection of PEG-PEI/Fe3O4 nanometer magnetic fluid under the alternating magnetic field. At the frequency of 40 kHz, and magnetic field of 5 kA/m, 15 minutes one day in the next 14 days. On the 7th day and the 15th day, the changes of tumor volume and weight were recorded, cell apoptosis were observed and recorded and pathological examination was done. Results On the 7th and the 15th day, in the nanometer magnetic fluid group, tumors' volume was smaller and the weight was lighter than other groups, and the tumor inhibitory rate of 54. 20% (t = 14. 506,P ＜0. 01 ) was significantly higher than the control group and the magnetic field group 22. 66% ( t = 7.497, P ＜ 0. 05 ). In the control group, tumor cells grew well, high density, the nucleus engrained, the shape irregular, the nuclear fission clear; compared with the control group, in the magnetic field group, tumor cells scatter thinly, intercellular substance increases, and necrosis area formed;in the nanometer magnetic fluid group, many of tumor cells died, their cell nucleus broke up and vanished,the blood vessel reduced obviously, and the tumor cell spread thinly. Conclusions Under the alternating magnetic field, PEG-PEI/Fe3O4 nanometer magnetic fluid inhibits liver cancer growth in nude mice model of HepG2.

Objective To study the characteristics of craniocerebral injury caused by the handgun bullet impacting on the bulletproof helmet.Methods Fourteen healthy landrace pigs were involved and randomly divided into injury group(n =9)and control group(n =5).The landrace pigs of the injury group were shot vertically on the head under the protection of helmet plate with 9 mm handgun bullet at velocity of 360 m/s.While the landrace pigs of the control group were dealt with the same process as the injury group except for use of unarmed handgun bullet of the same ammunition dose.The changes of vital sign in the early period and the retina injury at two hours after injury were observed.Porcine cerebrospinal fluid (CSF)at pre-injury and at three hours post-injury were obtained for investigating the levels of neuron specific enolase(NSE)andαⅡ-spectrin protein.Then,the landrace pigs were sacrificed and dissected to examine the general morphological changes of the brain.The brain cortex was taken,fixed and stained with hematoxylin-eosin for microscopic observation.Results The landrace pigs in the injury group manifested decrease of the blood pressure and increase of the heart rate and respiratory rate in the early stage after injury.General morphological observation found retinal hemorrhage(3/9),skull fracture(3/9)and brain surface damage including local impact lesion(9/9)and contrecoup lesion(9/9),with severe impact lesion than contrecoup lesion.Optical microscopic observation revealed acute injury of the cerebral cortex neuron both on the impact and contrecoup locations.The concentrations of NSE and αⅡ-spectrinwere significantly increased in CSF three hours after injury(P ＜ 0.05).Conclusions The handgun bullet impacts on the pig head protected by the bulletproof helmet may induce blunt craniocerebral injury in the early period,with severe impact lesion than contrecoup lesion.Therefore,traumatic brain injury of the soldiers armed with the helmets should be stressed and managed early.