Nursing simulation teaching is one of the important measures in nursing teaching reform. As an advanced medical teaching model, SimMan synthetic simulation has been recognized by medical educators both at home and abroad for its advantages of high simulation, repeatability, case diversity and process controllability. Our country has relied on the SimMan synthesis simulation person to carry on the nursing simulation teaching for many years, usually as a teaching strategy into the course of teaching. Its advantages and innovation are affirmed by nursing educators and learners, but still face challenges in the design of simulation teaching, the development of functions and the improvement of equipment utilization. It is worthy discussing deeply by nursing educators.

Objective To study the prognosis in the patients with distant metastasis after nasopharyngeal carcinoma radical radiotherapy and to analyze its influencing factors .Methods One hundred and fifty patients with distant metastasis after nasopha-ryngeal carcinoma radical radiotherapy were followed up .Their clinical data were collected to conduct the prognosis analysis and in-fluencing factors analysis .Results The median survival time in the follow up cases was 13 .50 months ,the one-year survival rate was 49 .8% ,two-year survival rate was 31 .0% and three-year survival rate was 19 .3% .The Cox model single factors analysis re-sults showed that the N stage in initial diagnosis ,whether conducting chemotherapy in initial diagnosis ,time interval from radiother-apy finish to distant metastasis occurrence ,metastasis site ,chemotherapy after distant metastasis ,chemotherapy cycles and palliative radiotherapy were the prognosis related influencing factors (P<0 .05) ,in which the N stage ,metastatic site ,chemotherapy after dis-tant metastasis ,time interval from radiotherapy finish to distant metastasis occurrence and palliative radiotherapy were the inde-pendent influencing factors of prognosis(P<0 .05) .Conclusion The independent factors of prognosis in the patients with distant metastasis after nasopharyngeal carcinoma radical radiotherapy are the N stage at initial diagnosis ,metastatic site ,whether having chemotherapy after distant metastasis ,time interval from radiotherapy finish to distant metastasis occurrence and whether conduc-ting the palliative radiotherapy .

Objective To observe the activity of histone deacetylase and the mRNA expression level of HDAC1 and HDAC2 in human bone marrow mononuclear cells,which induced by hydroquinone and exposed to hydroquinone plus Trichostatin as a histone deacetylase inhibitor for 10 hours respectively.Methods Collect the bone marrow mononuclear cells suspension,divided into control group,HQ group (3 h,6 h,12 h,24 h),HQ+ TSA 10 h group and HQ 10 h group.Extract the nuclear proteins and RNA,test the activity of histone deacetylase with the colorimetric HDAC assay kit and detect the mRNA expression level of HDAC 1 and HDAC2 by real-time Polymerase Chain Reaction(PCR).Results The HDAC activity of HQ3 h group,HQ6 h group and HQ12 h group were 1.31 times,1.53 times and 1.148 times than that of control group respectively.And the difference was statistically significant (P＜0.05).Except the HQ24 h group (P＞0.05),the HDAC1 mRNAexpression of HQ3 h group,HQ6 h group and HQ12 h group were 1.173 times,1.901 times and 2.348 times than that of control group respectively.And the difference was statistically significant (P＜0.05).The HDAC2 mRNA expression of HQ6 h group and HQ12 h group were 1.426 times and 1.766 times than that of the control group respectively.And the difference was statistically significant (P＜0.05).No significant difference was observed between HQ3 h group,HQ24 h group and control group(P＞0.05).The cells were treated by hydroquinone plus TSA for 10 hours.The HDAC activity of HQ+TSA 10h group was reduced by 25.6％ than that of HQ 10 h group (P＜0.05) and rised 13.0％ compared to the control group (P＜0.05).And the difference was statistically significant between groups (P＜0.05).The cells were treated by hydroquinone plus TSA for 10 hours.The HDAC 1 mRNA expression of the HQ+TSA 10h group is reduced by 26.9％ than that of HQ10h group.The HDAC2 mRNA expression is reduced by 19.3％ compared to the HQ 10h group.And the difference was statistically significant between groups (P＜0.05).The HDAC1 and HDAC2 mRNA expression is obviously higher than the control group,the difference was statistically significant (P＜0.05).Conclusion Treatment of hydroquinone,the histone deacetylase activity and the mRNA expression of HDAC1 and HDAC2 were increased in a certain time range.The histone deacetylase inhibitor (TSA) can reduce the histone deacetylase activity and the mRNA expression level of HDAC 1 and HDAC2 in the bone marrow mononuclear cell induce by hydroquinone.It can be confirmed that hematopoietic damage induced by the benzene metabolites is related to the histone acetylation modification level.

Objective To observe the activity of histone deacetylase and the mRNA expression level of HDAC1 and HDAC2 in human bone marrow mononuclear cells,which induced by hydroquinone and exposed to hydroquinone plus Trichostatin as a histone deacetylase inhibitor for 10 hours respectively.Methods Collect the bone marrow mononuclear cells suspension,divided into control group,HQ group (3 h,6 h,12 h,24 h),HQ+ TSA 10 h group and HQ 10 h group.Extract the nuclear proteins and RNA,test the activity of histone deacetylase with the colorimetric HDAC assay kit and detect the mRNA expression level of HDAC 1 and HDAC2 by real-time Polymerase Chain Reaction(PCR).Results The HDAC activity of HQ3 h group,HQ6 h group and HQ12 h group were 1.31 times,1.53 times and 1.148 times than that of control group respectively.And the difference was statistically significant (P＜0.05).Except the HQ24 h group (P＞0.05),the HDAC1 mRNAexpression of HQ3 h group,HQ6 h group and HQ12 h group were 1.173 times,1.901 times and 2.348 times than that of control group respectively.And the difference was statistically significant (P＜0.05).The HDAC2 mRNA expression of HQ6 h group and HQ12 h group were 1.426 times and 1.766 times than that of the control group respectively.And the difference was statistically significant (P＜0.05).No significant difference was observed between HQ3 h group,HQ24 h group and control group(P＞0.05).The cells were treated by hydroquinone plus TSA for 10 hours.The HDAC activity of HQ+TSA 10h group was reduced by 25.6％ than that of HQ 10 h group (P＜0.05) and rised 13.0％ compared to the control group (P＜0.05).And the difference was statistically significant between groups (P＜0.05).The cells were treated by hydroquinone plus TSA for 10 hours.The HDAC 1 mRNA expression of the HQ+TSA 10h group is reduced by 26.9％ than that of HQ10h group.The HDAC2 mRNA expression is reduced by 19.3％ compared to the HQ 10h group.And the difference was statistically significant between groups (P＜0.05).The HDAC1 and HDAC2 mRNA expression is obviously higher than the control group,the difference was statistically significant (P＜0.05).Conclusion Treatment of hydroquinone,the histone deacetylase activity and the mRNA expression of HDAC1 and HDAC2 were increased in a certain time range.The histone deacetylase inhibitor (TSA) can reduce the histone deacetylase activity and the mRNA expression level of HDAC 1 and HDAC2 in the bone marrow mononuclear cell induce by hydroquinone.It can be confirmed that hematopoietic damage induced by the benzene metabolites is related to the histone acetylation modification level.