Objective To investigate the effect of alprostadil injection on serum CRP, IL-6 and TNF-alpha and prognosis in patients with acute cerebral infarction.Methods 80 cases of patients with acute cerebral infarction from February 2014 to February 2016 in Ningbo Yinzhou People's Hospital were selected,all patients were randomly divided into two groups, the control group 40 cases, the conventional treatment of acute cerebral infarction;40 cases in the observation group, with alprostadil injection on the basis of control group.The levels of serum CRP, IL-6 and TNF-αwere observed before and after treatment, and the NIHSS and Barthel index score and the curative effect after treatment were observed.Results Compared with before treatment, no significant change after treatment of serum CRP, IL-6 and TNF-αlevels in the control group,the serum CRP, IL-6 and TNF-αlevels decreased significantly in the observation group ( P <0.05 ) , and significantly lower than the control group ( P <0.05 ).Compared with before treatment, NIHSS score decreased after treatment in two groups (P <0.05),Barthel index (P <0.05) score increased, NIHSS score in observation group was significantly lower than the control group (P<0.05), Barthel index score was significantly higher than the control group (P<0.05).After treatment,the effective rate of the observation group was 95%, which was significantly higher than that of the control group 72.5% ( P <0.05 ) . Conclusion Alprostadil injection treatment of acute cerebral infarction, can effectively reduce the patient's serum CRP, IL-6 and TNF-αlevels, reduce brain damage, can improve the curative effect, improve the prognosis.

Objective To evaluate the effect of a new one-piece PHEMA keratoprosthesis(Kpros) implanted in alkali burned rabbit corneas. Methods The experiment included two parts. In part one, the porous discs of PHEMA were inserted into the lamellar pockets of alkali-burned corneas and the results were observed clinically and histologically at various periods. In part two, seven one-piece Kpros were implanted into alkali burned rabbit corneas. The Kpros consisted of a central transparent optical area and a porous semi-transparent peripheral skirt. The surgical procedure was similar to that of penetrating keratoplasty. Clinical observations were carried out till 28 weeks. Results Both the porous discs and one-piece Kpros had been retained during the experiment. Severe complications as cataract, retro corneal membrane, retinal detachment and extrusion of material were not found until 28 weeks. There was no calcification in the samples, and inflammation was mild. Two weeks after implantation, fibroblasts and deposition of collagen were found in the pores of PHEMA discs. The fibroblasts in the pores look healthy and functionally active. The preliminary results were satisfactory. Conclusion This new one-piece Kpros had shown good biocompatibility. The porous peripheral skirt fuses well with the bed of burned cornea and proved to be stable. PHEMA materials is suitable for one-piece Kpros.

Objective To investigate the regulations of lymphatic metastasis of rectal carcinoma which can provide help of clinical diagnosis and treatments.Methods A retrospective analysis was performed of 222 patients with rectal carcinoma who underwent surgical resection from November 2006 to January 2012.Summarize the relevance of each factor and lymph node metastasis by logistic regression analysis.Results (1) Gender and lymph node metastasis has no significant correlation (x2 =0.000,P ＞ 0.05).(2) Lymph node metastasis rate reduced with increasing age and the difference was statistically significant (x2 =5.001,P ＜ 0.05).(3) There is no significant difference between tumor location and lymph node metastasis rate (x2 =0.052,P ＞ 0.05),when the distance between anal rectal cancer and the anus was closer,the tendency of lymph node metastasis was higher,and there has the obvious correlation with lymph node metastasis rate of its size,2.5 to 5.5 cm group and more than 5.5 cm lymph node metastasis rate was significantly higher than less than 2.5 cm group with a significant difference (x2 =2.267,P ＜ 0.05).(4) Tumor invasion of the intestinal circumference was closely related with lymph node metastasis rate (x2 =2.323,P ＜0.01).(5) Lymph node metastasis rate was closely related to the depth of invasion,metastasis rate increased with the depth of invasion increasing(x2 =2.277,P ＜0.05).(6) Lymph node metastasis was significantly higher with the decrease of the degree of tumor differentiation (x2 =4.322,P ＜ 0.01).Logistic regression analysis showed that the degree of tumor differentiation,infiltration and invasion of intestinal circumference diameter lymph node metastasis influential,OR (95 ％ CI) was 5.562 (0.105-0.444),2.178 (0.144-0.971),1.563 (0.038-8.613) respectively (P ＜0.01).Conclusion The lymphatic metastasis of rectal carcinoma is related to age,tumor size,intestinal circumference invasion,depth of tumor invasion and the differentiating degree of the tumor,and the differentiating degree is the major factor.

Objective To investigate the expression of lung keratinocyte growth factor receptor (KGFR) in rats with acute spinal cord injury (ASCI) in different time points and its role in lung edema.Methods Thirty-two adult Wistar rats weighing 240 g to 260 g were assigned to experimental group (n =16) and control group (n =16) according to the random number table.Each group consisted of time points of 24 hours,3 days,1 week and 2 weeks after the modeling (4 rats per time point).A rat model of ASCI in experimental group was induced at C7 segment by dropping a weight of 10 g from the height of 2.5 cm (Allen' s method).In control group,laminas were removed only,leaving spinal cord at C7 intact.Rats were sacrificed at each time point for measurement of lung wet/dry weight ratio,Western blot analysis of expression of lung KGFR protein and RT-qPCR detection of lung KGFR mRNA expression.Results After ASCI in rats,the expressions of lung KGFR protein and mRNA began to drop at 24 hours (0.23 ±0.06,0,012 1 ±0.002 3),reached the trough at 3 days (0.17 ±0.04,0.008 5 ±0.001 7)and picked up at 1 week.Expression of lung KGFR mRNA in experiment group showed statistically significant difference from that in control group at 24 hours and 3 days (P ＜ 0.05),whereas in each time point the difference of KGFR protein expression between experiment and control groups was statistically significant(P ＜0.05).Variation trend of KGFR expression was in parallel with the severity degree of pulmonary edema.Conclusion Lung KGFR presents significant down-regulation in ASCI rats and this may be associated with the development of pulmonary edema after ASCI.

Objective To report a case of lymphomatoid papulosis(LyP)followed by mycosis fun-goides(MF)during his27years course and determine whether these diseases are clonally related.Methods Characteristics of clinicopathology and immunophenotype of skin tissues of different phases of the patients were compared.Simultaneously,clonal T-cell receptor gene rearrangement of skin lesion tissues and blood specimens were detected by means of polymerase chain reaction and Southern blot analysis.Results A dis-tinct clinical and pathological feature was shown in this case of LyP followed by MF.The proliferating lym-phocytes in LyP and MF were mature T helper lymphocytes,and with same T-cell clone.Conclusion LyP followed by MF displays a same T-cell clone.The clonal T-cell is irrelevant to the biological behavior of these diseases.

OBJECTIVE To analyze the incidence of the bifurcation of the facial nerve in mastoid segment and to give the anatomic information for mastoid surgery. METHODS From March 2005 to April 2006, there were 112 patients undergoing the decompress operations which the range included the mastoid segment. The bifurcation of the mastoid segment was recorded and compared with the preoperational High-resolution Computed tomography (HRCT) scans. RESULTS There were 5 cases with the nerve bifurcation. The incidence was 4.5 %. Four of those 5 cases were diagnosed preoperatively by HRCT scans (the accurate rate was 80 %). CONCLUSION Bifurcation of the mastoid segment was not rare. Otologic surgeon should pay attention to this anatomic variation to avoid injuring the branch of the facial nerve. HRCT scans were useful to find this abnormality.

Objective To report the first case of cutaneous type adult T-cell leukemia/lymphoma(cATLL) in China. Methods The skin lesion was examined by histopathology and direct immunofluorescence (DIF), and the immunophenotype was also studied. ELISA and Western blot were used to test the serum antibodies to HTLV-Ⅰ, and HTLV-Ⅰproviral DNA of lymphoid cells was detected by PCR. Results The patient had polymorphic skin lesions including papules, plaques, and bullae with tense or flaccid walls. Histopathogical examination showed subepidermal bullae, and there were small-to-medium-sized atypical lymphoid cell infiltrations in the dermal papilla in the bottom, and border of the bulla. CD45+ and CD45RO+ staining, and negative DIF were observed in the atypical lymphoid cells. The serum antibodies to HTLV-Ⅰ, and proviral DNA of HTLV-Ⅰin the blood lymphoid cells were detected. The patient died with a disease course of one year and ten months. Conclusion ATLL is not extremely rare in China, and cATLL may also exist.

Objective To explore the influence of minimally invasive puncture drainage on blood brain barrier (BBB) function and its mechanism.Methods Ninety-two patients with hypertensive intra-cerebral hemorrhage (HICH) in the Department of Neurosurgery of Jiaxing Affiliated Second Hospital of Zhejiang Province were divided into a control group and an observation group, according to random number table method, 46 cases in each group. In the control group, the conventional craniotomy was performed, while in the observation group, minimally invasive puncture drainage was carried out to remove the hematoma. The National Institute of Health Stroke Scale (NIHSS) were used to evaluate the neural function, the level of serum myelin basic protein (MBP) was detected by enzyme linked immunosorbent assay (ELISA), the central nervous specific serum protein S100 level was measured by electrochemical luminescence method, the albumin levels in serum and cerebrospinal fluid were determined by automatic biological analyzer, and the BBB index was calculated. After 14 days of surgery, the curative effect and incidence of complications of two groups were observed.Results After surgery, the NIHSS scores of two groups were obviously lower than those before surgery, and the degree of descent in observation group was more significant than that in the control group (score: 3.68±2.39 vs. 5.43±3.89,P < 0.05); after surgery, the levels of MBP, S100, albumin in cerebrospinal fluid and BBB in two groups were higher than those before surgery [MBP (μg/L): 3.02±0.28 vs. 3.81±0.29, S100 (μg/L): 0.95±0.24 vs. 1.34±0.27, cerebrospinal fluid albumin (μg/L): 9.89±0.78 vs. 21.43±1.14, BBB index: 0.22±0.04 vs. 0.48±0.05], the differences being statistically significant (allP < 0.05), but the change values in the observation group were less significant than those in the control group. The total effective rate in observation group was significantly higher than that in the control group [84.78% (39/46) vs. 65.22% (30/46),χ2 = 4.696,P = 0.030]. The incidence of wound infection, gastrointestinal bleeding in observation group was markedly lower than that in the control group [16.67% (6/46) vs. 36.96% (17/46), χ2 = 4.120,P = 0.042].Conclusion The minimally invasive puncture drainage has unequivocal clinical curative effect in treatment of patients with HICH, it can protect the nerve and BBB functions and reduce the incidence of complications.

Besides cyclooxygenase and nitric oxide synthase, another distinct endothelial pathway, endothelium-dependent hyperpolarization factor (EDHF), is involved in relaxation of the vascular smooth muscle cells. EDHF has been demonstrated unequivocally in various blood vessels from different species, including human, and is likely to play an important role in cardiovascular physiology and pharmacology.

Aim To study the effect of total flavones of Rhododendra(TFR)on bioelectricity changes of ventricular myocytes in guinea pigs. Methods Conventional microelectrode technique was used to record action potentials (AP) , resting potentials (RP) and effective refractory period (ERP). Results TFR 25,50 mg?L-1 decreased the action potential duration at 50%,90% repolarization (APD50,APD90) while TFR 100,200,400 mg?L-1 prolonged APD50 and APD90Significantlg. TFR 200,400 mg?L-1markedly decreased action potentials amplitude (APA) and velocity maximum of depolarization (Vmax). TFR 400 mg?L-1decreased resting potential. TFR 200 mg?L-1 prolonged ERP. Conclusion TFR may antagonize Ca2+ inward flow at lower concentration and block K+ inward flow at higher concentration.