1.Ultrastructural changes of rat retinal sensitive cell in critical period of visual development
Yongfeng YANG ; Hanjun SUN ; Yide HU
Journal of Third Military Medical University 2003;0(14):-
Objective To observe the changes of retinal sensitive cellular ultrastructure of rats in critical period of visual development.Methods Ten normal healthy Wistar rats were chosen,eight of which were neonatal rats and two were mature rats.The eight neonatal rats were randomized into four groups(n=2 in each group) and respectively sacrificed on postborn day 0,15,20,25.The eyeballs of the neonatal rats and the mature rats were resected and the retinas were observed by electron microscope.Results The rat retinal sensitive cellular ultrastructure on postborn day 0 was immature and the cellular arrangement was not clear.The organelle of sensitive cell in critical period developed mature gradually and the arrangement of them was very clear.Conclusion The retinal sensitive cell of rats develops and matures gradually in critical period.
2.The investigation of the influence of age on liver histopathological feature of patients with chronic hepatitis B
Hongdong XIE ; Ying YU ; Qian JIN ; Yide YANG ; Wei HOU
Journal of Chinese Physician 2010;12(12):1639-1642
Objective To investigate the influence of age on liver histopathological feature of patients infected with chronic hepatitis B. Methods Liver biopsies were performed on 114 patients with chronic hepatitis B. The biochemical tests were measured by routine automated techniques. Serum hepatitis markers including HBsAg, anti-HBs, HBeAg, anti-HBe, and anti-HBc were assayed by using a microparticle enzyme linked immunosorbent assay. HBV-DNA was measured with quantitative polymerase chain reaction assay. The grades of liver necrosis/inflammation and fibrosis were compared between four groups with different age: younger than 20years, 20 ~ 30, 31 ~40, and older than 40 years. Results All 114 patients had liver histological changes with different degree. 75.4 percent (89/114) of cases had 2/over 2 grades of liver necrosis/inflammation, and 47.4 percent (54/114) of cases had 2/over 2 stages of liver fibrosis.There was no significant relation between the level of ALT and age or between the level of ALT and the grades of liver necrosis/inflammation and fibrosis( P >0. 05 ). The significant difference in degrees of liver inflammation and fibrosis was found among three age groups ( x2 = 30. 86, P < 0. 01; x2 = 21.17; P <0.05 ). The grades of 1 iver inflammation and fibrosis increased with the increased age of the patients. Conclusion These results suggested that age was an independent factor for the disease progression. It was very important to undertake liver biopsy for patients with CHB more than 30 years to reveal the liver histopathological characteristics and guide the treatment.
3.Analysis on awareness of obesity risk factors among children and adolescents
Lianguo FU ; Haijun WANG ; Yide YANG ; Xiaohui LI ; Shuo WANG ; Xiangkun MENG ; Zhenghe WANG ; Jun MA
Journal of Peking University(Health Sciences) 2015;(3):410-413
Objective:To analyze the awareness rate of obesity risk factors among children and adoles-cents, and to provide the basis for preventing their obesity. Methods: The students selected by using stratified cluster sampling method, were measured for the body height, weight, and waist circumference, and were surveyed for the awareness rate of obesity risk factors by using the questionnaire. The distribu-tion characteristics of awareness on obesity risk factors were analyzed. Results: The awareness rate of obesity risk factors was 74 . 1%, and there was no significant difference on the awareness rate of obesity risk factors between the males [71. 2% (247/347)] and females [77. 2% (257/333)], the abdominal obesity students [73. 5% (202/275)] and non-abdominal obesity students [74. 6% (302/405)], the overall obesity students [74. 3% (185/249)] and non-overall obesity students [74. 0% (319/431)], re-spectively. However, the awareness rate of obesity risk factors among the primary school students was 81. 9% (272/332) that was higher than that of the middle school students [66. 7% (232/348)] (P<0. 05). Compared with the primary school students, the non-awareness risk of obesity risk factors would in-crease among the middle school students (OR=2. 23, P<0. 05). Conclusion:The overall awareness rate of obesity risk factors is not high among children and adolescents, especially among middle school students.
4.Diagnosis and treatment for 17 isolated iliac aneurysms
Yide ZHENG ; Li FANG ; Xiaowen FAN ; Hui YANG ; Yongzhong YU ; Lijuan CHEN
Chinese Journal of General Surgery 2012;27(5):395-397
ObjectiveTo summarize the experiences in surgical and endovascular treatment for isolated iliac artery aneurysm.MethodsData of 17 cases with isolated iliac artery aneurysm were reviewed and analysed retrospectivel.17 cases were examined with color Doppler ultrasounograpy preoperatively,13 cases underwent computed tomographic angiography (CTA),2 cases did digital subtraction angiography (DSA).Artificial graft interposition were performed in 10 cases with unilateral iliac artery aneurysm,endovascular repair were performed in 6 cases.One case was treated by artificial graft interposition after a failed endovascular repair. ResultsPreoperativaly correct diagnosis was established in all 17 cases,and they were cured after operation.The average operative time was (3.7 ± 1.2) h in group of surgical operation,and ( 1.4 ±0.7) h in group of endovascular treatment (P <0.05).The length of hospital stay was ( 16 ± 5 ) d for the group of surgical operation,and ( 9 ± 4 ) d for endovascular treatment ( P < 0.05 ).The percentage of blood transfusion was 72.7% ( 8/11) in the group of surgical operation,and 16.7% (1/6) in the group of endovascular treatment.There were a ureteral injury and a ectopic embolism happened during the procedure.There were 2 hip claudication and 2 type Ⅰ endoleaks after operation,which subsided after 3 months.ConclusionsArtificial graft interposition or endovascular repair is effective treatment for isolated iliac artery aneurysm which has the advantage of minimally invasiveness and rapid recovery.
5.Application of digital subtraction angiography in ENT disorders
Xiaohua SHEN ; Shuimiao ZHOU ; Yide ZHOU ; Ping PENG ; Desheng JIANG ; Zhentang WANG ; Jijin YANG ; Wu WEN ; Feng LIU ; Zhaoji LI
Academic Journal of Second Military Medical University 2001;22(4):346-348
Objective: To study the application of DSA in the diagnosis and treatment of ENT diseases. Methods: The diagnostic and therapeutic roles of DSA in ENT patients admitted from November 1995 to December 1999 were retrospectively studied. Results: Therapeutic vascular embolization using DSA was performed in 9/10 patients with severe epistaxis. The treatment was successful in 8/9 patients with a successful rate of 88.89%; embolization of tumor supplying vessels using DSA as a preoperative measure for reducing operative blood loss in 3 patients with nasopharyngeal fibrohemangioma obtained a total success; diagnosis was clarified in 2 patients using DSA. No patients were with severe complications. Conclusion: DSA is not only a safe and effective measure for diagnosis and therapy, but also effective in differential diagnosis of space occupying lesions. Preoperative selective embolization of tumor supplying arteries can reduce operative blood loss.
6.Analysis of differences in anthropometric measurements between Mongolia and Han girls in China.
Lianguo FU ; Lili SUN ; Yide YANG ; Xiaohui LI ; Shuo WANG ; Xiangkun MENG ; Zhenghe WANG ; Jun MA
Chinese Journal of Preventive Medicine 2015;49(4):344-349
OBJECTIVETo analyze the differences in anthropometric measurements between Mongolia and Han girls and understand the characteristics of their physical development in two ethnic groups.
METHODSThe data on anthropometric measurements of Mongolia and Han girls aged 9-17 years in Inner Mongolia were obtained from the Chinese National Survey on Student's Constitution and Health (CNSSCH) in 2010, and the data included body height, sitting height, chest circumference, weight, triceps skinfold thickness (TST), scapular skinfold thickness (SST), abdominal skinfold thickness (AST), and having menarche or not. The girls were divided into two groups according to having menarche or not, and the differences in anthropometric measurements between Mongolia and Han girls were analyzed for the two groups, respectively.
RESULTSThe proportions of Mongolia girls (9-17 years) who have experienced menarche was higher than that of Han girls (67.6%,1 049/1 553) vs (62.9%,1 837/2 922) (χ² = 9.17, P = 0.002). In girls without menarche (9-14 years), sitting height, AST, and BMI in Mongolia girls was 0.5 cm, 1.6 mm, and 0.4 kg/m², respectively, which were higher than those in Han girls (all P < 0.05). In girls who have experienced menarche (11-17 years), height and SST in Han girls was 0.8 cm, and 1.0 mm, respectively, which were higher than those in Mongolia girls (all P values < 0.01); TST, AST and BMI in Mongolia girls were 0.9 mm, 2.9 mm, and 0.3 kg/m², respectively, which were higher than those in Han girls (all P values < 0.05), respectively.
CONCLUSIONBody AST and BMI in Mongolia girls are higher than those in Han girls, while body height in Han girls is better than that in Mongolia girls after menarche. The differences of physical development between Mongolia girls and Han girls might be different before and after experiencing menarche.
Adolescent ; Anthropometry ; Asian Continental Ancestry Group ; Body Height ; Body Mass Index ; Body Weight ; Body Weights and Measures ; Child ; China ; Female ; Humans ; Menarche ; Skinfold Thickness
7.Apoptotic regulation and tRNA.
Yide MEI ; Aaron STONESTROM ; Ya-Ming HOU ; Xiaolu YANG
Protein & Cell 2010;1(9):795-801
Apoptotic regulation is critical to organismal homeostasis and protection against many human disease processes such as cancer. Significant research efforts over the past several decades have illuminated many signaling molecules and effecter proteins responsible for this form of programmed cell death. Recent evidence suggests that transfer RNA (tRNA) regulates apoptotic sensitivity at the level of cytochrome c-mediated apoptosome formation. This finding unexpectedly places tRNA at the nexus of cellular biosynthesis and survival. Here we review the current understanding of both the apoptotic machinery and tRNA biology. We describe the evidence linking tRNA and cytochrome c in depth, and speculate on the implications of this link in cell biology.
Animals
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Apoptosis
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genetics
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physiology
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Caspase 9
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physiology
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Cytochromes c
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physiology
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Humans
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Models, Biological
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Nucleic Acid Conformation
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RNA, Transfer
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chemistry
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genetics
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physiology
8. Comparison whole-body muscle mass assessed by dual-energy X-ray absorptiometry and multi-frequency bioelectrical impedance in overweight and obese adults
Zhenghe WANG ; Yanhui DONG ; Yide YANG ; Shuo WANG ; Jun MA
Chinese Journal of Preventive Medicine 2017;51(3):215-219
Objective:
To investigate consistency of whole-body muscle mass (WBMM) assessed using multi-frequency bioelectrical impedance analysis (MF-BIA) and dual energy X-ray Absorptiometry (DXA) measurement, and to delvelop the correction regression formulas for the method of MF-BIA.
Methods:
1 488 participants who resided in Beijing longer than one year, under 22 to 55 years of age, with self-report BMI ≥24 kg/m2 were voluntarily recruited based on convenience sampling in Beijing, from April 1st 2014 to May 2nd 2014. After excluded 171 participants who BMI ≤24 kg/m2 or with organic diseases when physical examination, Finally 1 317 overweight/obese participants were enrolled into the current study. All the subjects received the measurement of WBMM using both MF-BIA and DXA methods. The paired-samples T test was used to compare the difference of measurement values between MF-BIA and DXA methods. We evaluated the agreement of WBMM measured by MF-BIA and DXA using the interclass correlation coefficients (ICC) and the Bland-Altman plots, and using linear regression method established correction regression formulas.
Results:
The differences of overweight male, obese male, overweight female, and obese female were (3.29 ± 1.94), (2.05 ± 2.65), (0.17 ± 1.85), and (-2.22 ± 2.89) kg between methods of MF-BIA and DXA, respectively; Except the overweight female, the others groups had the statistically significant difference (
9. Analysis of association between appendicular skeletal muscle mass index and main anthropometric parameters among overweight and obese adults in China
Zhenghe WANG ; Yide YANG ; Shuo WANG ; Yanhui DONG ; Xijie WANG ; Jun MA
Chinese Journal of Preventive Medicine 2017;51(8):746-750
Objective:
To investigate the associations between the appendicular skeletal muscle mass index and main anthropometric parameters, and to provide clues for preventing the reduction of skeletal muscle mass among overweight and obese adults in China.
Methods:
1 488 volunteered participants who resided in Beijing longer than one year, between 22 and 55 years old, with self-report BMI ≥24 kg/m2 were recruited from April to May 2014. 171 participants whose BMI ≤24 kg/m2 or with organic diseases at the first physical examination were excluded, finally 1 317 overweight/obese participants (male and female were 507, and 810, respectively) were enrolled into the present study. After measuring the anthropometric parameters and body composition by the DXA, Pearson correlation analysis and multiple linear regression models were used to analyze the associations between the appendicular skeletal muscle mass index and main anthropometric parameters.
Results:
The average age of males and females was (35.6±8.4) and (38.2±9.4) years old, respectively. The females' mean levels of body fat percentage, BMI, waist circumference, hip circumference, and bone mineral density were (41.59±4.14)%, (29.00±3.75) kg/m2, (92.56±9.00) cm, (101.74±6.75) cm, and (1.20±0.10) g/cm2, respectively; and the males' mean levels were (34.22±4.61)%, (30.07±4.12) kg/m2, (99.63±9.49) cm, (102.93±7.28) cm, and (1.28±0.11) g/cm2, respectively. The mean level of appendicular skeletal muscle mass and skeletal muscle mass index in males were (26.98±3.88), and (8.96±1.02) kg, respectively, which were significantly higher than it in females ((18.57±2.61), and (7.26±0.83) kg, respectively) (
10. Association between polymorphism of rs10185316 in insulin-induced gene 2 and blood pressure among children and adolescents
Yide YANG ; Jieyun SONG ; Fanghong LIU ; Xiaorui SHANG ; Haijun WANG ; Jun MA
Chinese Journal of Preventive Medicine 2017;51(10):939-942
Objective:
To examine the association between polymorphism of rs10185316 in insulin-induced gene 2 (INSIG2) and blood pressure among children and adolescents.
Methods:
9 junior middle schools in Dongcheng District of Beijing and 5 schools (3 primary junior middle schools, 2 primary schools) in Haidian District of Beijing were chosen in 2005 and 2007, respectively. According to the Chinese BMI percentile criteria for screening overweight and obesity in school children, we recruited 1 425 overweight or obese children and 605 normal weight children. A total of 2 018 students with complete data of blood pressure and genotype data were included in this study. According to the blood pressure criterion of children and adolescents, 702 participants were categorized into high blood pressure group and 1 316 into normal blood pressure group. Participants' information of gender, age, height, weight and blood pressure were collected by questionnaire and physical examination. Genomic DNA was extracted from peripheral blood sample for genotyping of INSIG2 rs10185316 polymorphism. Multiple linear regression was conducted to analyze the associations between rs10185316 polymorphism in INSIG2 and SBP, DBP, mean arterial pressure (MAP) and pulse pressure.
Results:
The age, BMI, SBP and DBP of the high blood pressure group were separately (14.3±1.4) years old, (27.3±4.2) kg/m2, (130.5±10.9) and (76.7±13.3) mmHg (1 mmHg=0.133 kPa), all higher than that of the normal blood pressure group, which were (12.2±2.9) years old, (22.0±4.0) kg/m2, (104.4±10.9) and(54.6±15.2) mmHg, respectively (all