1.Urothroplasty by using the mucosa of diverticulum secondary to recurrence of urethral stricture
Yiqing LYU ; Hua XIE ; Ling YU ; Yichen HUANG ; Xiaoxi LI ; Fang CHEN
Chinese Journal of Urology 2015;36(5):361-364
Objective To assess the efficacy of urethroplasty by using the mucosa of diverticulum as the distal urethral for the recurrence of urethral stricture.Methods We reviewed our experience about 6 cases from Jan.2007 to Oct.2012,including 5 hypospadias and 1 epispadias.The mean age of the patients was 28 ± 16 (12-45)month,range 12 to 45 months.The patients presenting urethral diverticulum should last for 6 months after urethroplasty,which should last for 6 months.The preoperative mean urinary flow rate was 5.1 ± 1.4 (range 3.1 to 7.3) ml/s.All the patients took the retrograde urethrography and cystoscopy to confirm the position and length of urethrostenosis.The positions of urethrostenosis were at the penis coronary in 5 cases and glans penis in 1 case.The mean length of stricture was 13.3 ±4.2(range 8.8 to 20.5) mm.The mean length of diverticulum was 37.5 ± 15.3 (range 21.8 to 55.2) mm.All patients received the urethral reconstruction by using the pedicel diverticulum mucosa.After resecting the stenosis urethra and exposed the whole diverticulum,we opened the diverticulum laterally and halved it.One was used for tabularized urethroplasty in situ,the other was flipped to the distal urethral as pedicel flap to remedy the defect of the urethral.Results Totally 6 cases received the procedure.The mean operating time was 133 ± 48 (range 84 to 192) min.Postoperatively,urethral catheter was remained 2 weeks.All the patients can urinate smoothly after removing the catheter.The postoperative mean urinary flow rate was 10o 9 ± 3.3 (range 6.3 to 15.9) ml/s.The mean follow-up time was 2.2 yrs(range 1 to 4 years).At the end point of follow-up,all patients had not experienced a recurrent diverticulum or stricture at the anastomotic site.Urethrocutaneous fistula in the coronary glan was reported in 2 patients,which were finally cured after 2nd repair.No complication was found in other 4 patients.Conclusions Urethroplasty by flipping the mucosa of diverticulum takes full advantage of urinary mucosa that already existing,which can be very useful in those patients who are lack of foreskin.This approach can also reduce the patient's pain by completing the urethroplasty in situ and avoiding staging operation.But up till now,it can only be applied to the urethral diverticulum secondary to urethrostenosis.It also demands flip flap skilled.And the long-term effects and complications still remain to be seen.
2.Docetaxel-loaded nanoformulation based on star-shaped M-PLGA for cervical cancer therapy
Yichen DONG ; Xiaowei ZENG ; Wei TAO ; Yongfeng GAO ; Lin MEI ; Laiqiang HUANG
International Journal of Biomedical Engineering 2014;37(1):1-5,后插1
Objective Mannitol-poly(D,L-lactide-co-glycolide) (M-PLGA),a star-shaped biodegradable polymer,was synthesized with the intent in this research,to provide novel nanoformulation for cervical cancer chemotherapy.Methods The novel star-shaped copolymer was prepared by ring-opening polymerization,and characterized by NMR.The docetaxel-loaded M-PLGA nanoparticles,prepared by modified nano-precipitation method,were observed to be near-spherical shape with narrow size distribution.Results The CLSM results showed the uptake level of M-PLGA NPs was higher than PLGA NPs in Hela cells.A significantly higher level of cytotoxicity was achieved by docetaxel-loaded M-PLGA NPs than that of commercial Taxotere and docetaxel-loaded PLGA NPs,indicating that the star-shaped biodegradable polymer M-PLGA could be superior to the linear polymer PLGA as a drug carrier.The study on drug loading and encapsulation efficiency also proved that star-shaped M-PLGA could carry higher level of drug than linear polymer,therefore could be more efficient for cancer treatment.Conclusions In conclusion,the star-shaped M-PLGA copolymer may be used as a potential and promising drug-loaded biomaterial applied in developing novel nanoformulations for cervical cancer therapy.
3.Forefoot reconstruction in application of the reverse dorsal metatarsal artery perforator flap: 43 cases report
Haoran SUN ; Dongxu HUANG ; Yichen DOU ; Laijin LU ; Tao WANG ; Jingyan REN ; Xueyuan JIA
Chinese Journal of Microsurgery 2017;40(3):234-236
Objective To discuss the clinical application of reverse dorsal metatarsal artery perforator flap for reconstruction of forefoot soft tissue defects.Methods Since January,2008 to March,2016,43 patients with forefoot soft tissue defect due to various causes were reconstructed with the flap based on dorsal metatarsal artery perforator.Dorsal pedis was used as a donor site with dorsal metatarsal artery perforator as the donor artery.The flap size varied from 2.0 cm × 4.0 cm to 6.0 cm × 8.0 cm.Among them,27,8,4 and 4 cases were reconstructed with flap based on first,second,third and fourth dorsal metatarsal artery perforator flap respectively.Results All the patients were followed-up which ranged from 3 months to 24 months.Six flaps suffered from post operative venous congestion.Out of 6,4 survived with early emergency management while the remaining 2 suffered epidermal necrosis which survived with regular dressing change.Eventually,all the flaps survived.They had good texture and they were elastic,good-looking and very wear-resisting.Walking function was normal.Conclusion Reverse dorsal metatarsal artery perforator flap is an ideal choice in reconstruction of small to medium sized soft tissue defects of forefoot.
4.Study on the approach of promoting service trade of traditional Chinese medicine into Sri Lanka
Yichen HUANG ; Chengyu WANG ; Xinyang SONG
International Journal of Traditional Chinese Medicine 2019;41(1):5-7
Sri Lanka and China enjoy profound traditional friendship and active bilateral friendly cooperation. With the deepening of bilateral FTA negotiations, service trade of traditional Chinese enters into Sri Lanka is also promising. Though the current policy and law exists insufficiency, leveraging the FTA negotiations, TCM-Center binding Confucius Institute, providing TCM features and developing tour routes of medical tourism can help to push service trade of traditional Chinese medicine into Sri Lanka. The development of overseas Chinese medicine health services is not only benefit to the local, but also contribute to the new pattern of Chinese economic reformation.
5.Preliminary results of robotic-assisted laparoscopic pyeloplasty in children
Yiqing LYU ; Hua XIE ; Yichen HUANG ; Chuanliang XU ; Ling YU ; Xiaoxi LI ; Yan CHEN ; Zhi DING ; Ganggang YANG ; Li SUN ; Huizhen SUN ; Fang CHEN ; Yinghao SUN
Chinese Journal of Urology 2015;(10):721-725
Objective To evaluate the feasibility and outcomes of robotic-assisted laparoscopic pyeloplasty in children .Methods A retrospective study was performed in patients who underwent robotic-assisted laparoscopic pyeloplasty ( Anderson-Hynes ) at our institution between January 2014 to August 2014.Totally 6 boys were diagnosed as left ureteropelvic junction obstruction depending on the symptoms and radiographic studies .The mean age was 9 years ( range 4 -12 years ) .Results The procedure was performed successfully without conversion to open surgery in all of the cases .Mean operative time was 216 min (range 175-269 min), with a mean robotic anastomosis time of 45 min (range 30-60 min).Mean estimated blood loss was less than 15 ml.The mean hospitalization was 4.5 days.Mean follow-up period was 10 months ( range 7 -14 months ) .There were no perioperative complications , and recovery was uncomplicated (without recurrence, pyelonephritis, nephrarctia) in all of the patients.Conclusion Robotic-assisted laparoscopic pyeloplasty can be safely performed in children older than 4-year-old with ureteropelvic junction obstruction .
6.Live cell fluorescent imaging and cytotoxicity assessment of pH fluorescent probe based on styrylcyanine dyes.
Lixia HUANG ; Xiang LIU ; Danting CUI ; Yikai XU ; Wenwei YOU ; Yichen YAN ; Xinwei LU ; Ruiyuan LIU
Journal of Southern Medical University 2014;34(11):1642-1645
OBJECTIVETo prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging.
METHODSThe Probe 1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on the fluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as a pH fluorescence probe in living cell.
RESULTSProbe 1 emitted green fluorescence under neutral and basic conditions but orange fluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantly affecting the cell viability.
CONCLUSIONThe pH-sensitive fluorescent probe prepared based on styrylcyanine possesses good ability of cell membrane permeation for live cell fluorescent imaging.
Cells, Cultured ; Fluorescence ; Fluorescent Dyes ; Hydrogen-Ion Concentration ; Optical Imaging
7.Current situation and strategies on the development of traditional Chinese medicine service trade in Spain
Yichen HUANG ; Qin SHAO ; Xinyang SONG
International Journal of Traditional Chinese Medicine 2020;42(4):312-315
There is a long exchanges history between China and Spain. The acceptance of traditional Chinese medicine (TCM) and acupuncture therapy in Spain is increasing in recent years. However, the legal status of TCM has not yet been recognized by the Spanish government, who has a wait-and-see attitude towards acupuncture. The prospects of TCM and acupuncture are still unclear. We could try to gradually permeate TCM and Acupunction into Spain Health System by taking Chinese medicine insurance as a breakthrough, building Chinese medicine knowledge platform and optimizing overseas mergers and acquisitions so as to promote traditional Chinese medicine into the mainstream of Spain.
8.A comparative study of lumen-apposing metal stent and plastic stent for the treatment of pancreatic walled-off necrosis
Yichen QU ; Haitao HUANG ; Hangbin JIN ; Qifeng LOU ; Xiaofeng ZHANG ; Jianfeng YANG
Chinese Journal of Digestive Endoscopy 2022;39(8):635-640
Objective:To evaluate the efficacy and safety of lumen-apposing metal stent (LAMS) for the treatment of pancreatic walled-off necrosis (WON).Methods:A retrospective cohort study was performed on data of 43 consecutive patients with pancreatic WON who underwent endoscopic ultrasound-guided drainage by LAMS or plastic stents (PS) in Hangzhou First People's Hospital from December 2010 to June 2020. According to the type of stent used, the patients were divided into the LAMS group ( n=16) and the PS group ( n=27). The technical success rate, the clinical success rate, the operation time, the session of endoscopic necrosectomy, the stent insertion time and adverse events were compared between the two groups. Results:All 43 patients were successfully stented, indicating a technical success rate of 100% in both groups. For the LAMS group, the clinical success rate, the operation time, the session of endoscopic necrosectomy, the stent insertion time and overall incidence of adverse events were 75.0% (12/16), 26.0 (19.1, 39.8) min, 0.5 (0, 2.0) times, (41.3±28.4) days, and 43.7% (7/16), respectively, whereas these indices of the PS group were 37.0% (10/27) ( χ2=5.795, P=0.016), 31.0 (26.0, 48.0) min ( Z=1.221, P=0.222), 0 (0, 0) times ( Z=2.245, P=0.025), (176.1±99.1) days ( t=5.187, P<0.001) and 14.8% (4/27) ( χ2=8.893, P=0.064), respectively. Conclusion:LAMS placement is safe and effective for the treatment of pancreatic WON with a higher clinical success rate compared with PS. However, it requires more endoscopic intervention.
9.Short-term outcome of Kelly procedure for bladder exstrophy- epispadias complex
Yichen HUANG ; Yiqing LYU ; Hua XIE ; Ling YU ; Xiaoxi LI ; Li SUN ; Yan CHEN ; Huizhen SUN ; Ganggang YANG
Chinese Journal of Urology 2017;38(z1):29-33
Objective To evaluate the short-term outcome of the Kelly procedure for bladder exstrophy-epispadias complex.Methods Kelly procedure was performed in 5 cases from December 2015 in our institute. Retrospective analysis was done including the surgery and follow up.Results Two bladder exstrophy boys had histories of primary bladder closure without bladder neck reconstruction during the neonatal period. Another three epispadias boys had sever urethral defects with full incontinence. All cases undertook Kelly procedure and were followed up for 7.8 months (2-12 months). No severe complications such as glanular necrosis, urethral stricture and urethrocutaneous fistula was observed. Four cases were fully continent and one epispadias case had stress incontinence.Conclusions Kelly procedure produced satisfactory urinary continence and phallic cosmetics in bladder exstrophy-epispadias complex in children. Proper identification of the pudendal neurovascular bundle and repair of the external sphincter by levatorplasty help improve the outcome.