1.Puerarin attenuated ischemia/reperfusion injury an in vitro model through a repression of autophagy by HMGB1/TLR4/NF-κB axis
Yichen FAN ; Jiayi YAN ; Xueling LI ; Ni LIU ; Youhua ZHU ; Mingxing SUI ; Yifei ZHONG
Chinese Journal of Organ Transplantation 2021;42(9):549-553
Objective:To explore the protective effect of puerarin on hypoxia/reoxygenation (H/R)-induced acute kidney injury(AKI)in vitro.Methods:HK-2 cells were treated with H/R for simulating ischemia reperfusion injury(IRI)in vivo. The experimental groups included control group, H/R treatment group(0/6/12/24 h), H/R 24 h + puerarin treatment group(puerarin, Pue), H/R 24 h + Pue+ 3-methyladenine(3-MA)treatment group and H/R 24 h+ 3-MA treatment group. Immunoblotting was employed for detecting the expression changes of autophagy-related proteins, CCK-8 for examining cell proliferation, electron microscopy for observing autophagosome formation and TUNEL for detecting apoptosis.Results:As compared with control group, the expression of LC3-II rose in H/R 24 h group, the expression of autophagy marker P62 declined, count of autophagosome increased, cell viability decreased and cellular inflammation occurred. Puerarin had similar effects to 3-MA. As compared with H/R 24 h group, puerarin could reverse the changes in the expression levels of LC3 and P62 induced by H/R( P<0.05). There were greater cell viability, reduced autophagosome count and lessened cell apoptosis( P<0.05). At the same time, protein expression levels of HMGB1, TLR4 and NF-κB dropped( P<0.05). Conclusions:Puerarin suppresses autophagy through HMGB1/TLR4/NF-κB axis for lessening ischemia-reperfusion injury an in vitro model.
2.Clinical efficacy and safety of ex-vivo liver resection and liver auto-transplantation: a bayesian single-arm Meta-analysis
Yichen FAN ; Manjun DENG ; Jingxin YAN ; Li REN ; Haijiu WANG ; Haining FAN ; Qian LU
Chinese Journal of Hepatobiliary Surgery 2022;28(7):547-554
Objective:To evaluate the clinical efficacy and safety of ex vivo liver resection and autotransplantation (ELRA) by using a Bayesian single-arm Meta-analysis.Methods:Databases of PubMed, Embase, Cochrane Library, Web of Science, CNKI, and Wanfang were searched from January 1, 1990 to December 30, 2021 on ELRA studies. The Bayesian one-arm Meta-analysis was performed by using the statistical software of R (V4.1.2) and the Markov chain-Monte Carlo method was used to simulate the posterior distribution. The mortality rate within 30 days after operation, 1-year survival rate, major postoperative complications, R 0 resection rate and other related indexes were analyzed. Results:A total of 20 studies with 436 patients were included. Bayesian single-arm Meta-analysis showed that the 1-year survival rate after ELRA was 83.24% [95% highest posterior density ( HPD): 72.40%-92.05%]. The 1-year survival rates after surgery were 88.66% (95% HPD: 81.52%-94.50%) for patients with hepatic alveolar echinococcosis and 61.29% (95% HPD: 38.53%-93.68%) for patients with hepatic malignancies, respectively. The mortality rate within 30 d after surgery, the incidence of significant postoperative complications, and the R 0 resection rate were 6.96% (95% HPD: 4.47%-10.15%), 27.91% (95% HPD: 19.00%-38.30%), and 99.84% (95% HPD: 37.61%-100.00%), respectively. Renal failure was the most frequent cause of death after ELRA. Conclusion:ELRA is indicated for hepatic malignancies and hepatic alveolar echinococcosis when intrahepatic resection cannot be accomplished in vivo. The greatest benefit is observed in patients with hepatic alveolar echinococcosis, while only some patients with hepatic malignancies can benefit. The indications for ELRA for hepatic malignancies need to be further studied to define the subgroup of patients who can benefit from this operation.
3.Expression change of PTGFR gene in articular capsule and ligamentum teres in DDH and its correlation with pathogenesis of DDH
Lihua ZHAO ; Qing JIAO ; Mengjie CHEN ; Yichen WANG ; Lingyan FAN ; Feng MA ; Hao YING ; Sun WANG
Chongqing Medicine 2017;46(33):4613-4615
Objective To analyze the expression change of PTGFR gene in hip joint tissues of development dysplasia of the hip (DDH) and its correlation with DDH pathogenesis .Methods Eight age-and gender-matched children with DDH (DDH group) and control children (control group) were enrolled for conducting the compaison .The real-time quantitative PCR method and West-ern-blot method were adopted to detect the PTGFR mRNA and protein expression levels .Results PTGFR mRNA expression level in the hip articular capsule and ligamentum teres of the DDH group were significantly decreased compared with those of the control group (t=3 .472 ,2 .887 ,P<0 .05 ,) .The PTGFR protein expression level in the hip articular capsule and ligamentum teres of the DDH group were significantly decreased compared with those of the control group (t=5 .488 ,3 .942 ,P<0 .05) .Conclusion PTG-FR could play an important role in DDH pathogenesis and may be one of DDH pathogenic genes .
4.Relationship between different levels of central venous pressure and blood loss during pediatric living donor liver transplantation
Zhiying PAN ; Yichen FAN ; Lingke CHEN ; Xiaoqiang WANG ; Diansan SU ; Jie TIAN ; Liqun YANG ; Weifeng YU
Chinese Journal of Anesthesiology 2019;39(4):405-407
Objective To evaluate the relationship between different levels of central venous pres-sure (CVP) and blood loss during pediatric living donor liver transplantation (LDLT). Methods Pediat-ric patients underwent LDLT in Renji Hospital from 2006 to August 10, 2016 were retrospectively reviewed. The patients were divided into 2 groups according to the different CVP levels before peritoneum closure:central venous pressure<10 mmHg group ( group L) and CVP≥10 mmHg group ( group H) . The primary outcome measure was intraoperative blood loss. The secondary outcome measures were duration of mechani-cal ventilation in intensive care unit (ICU), duration of ICU stay, length of postoperative hospital stay, intraoperative blood loss, intraoperative blood transfusion, intraoperative volume of liquid infused, opera-tion time and anesthesia time. Results A total of 442 pediatric patients were enrolled in this study, with 209 cases in group L and 233 cases in group H. Compared with group H, the intraoperative blood loss was significantly decreased, the anesthesia time, operation time and length of postoperative hospital stay were shortened ( P<0. 05) , and no significant change was found in intraoperative blood transfusion, intraopera-tive volume of liquid infused, duration of mechanical ventilation in ICU or duration of ICU stay in group L ( P>0. 05) . Conclusion Maintaining intraoperative CVP<10 mmHg can markedly reduce the blood loss during LDLT and is helpful for postoperative recovery in pediatric patients.
5.Analysis of causes and solutions for vacuum suction weakness of dental units
Xinya LI ; Yichen WANG ; Yansong LIU ; Anjia ZHENG ; Shubin WU ; Baolin FAN ; Jianxia WANG
China Medical Equipment 2024;21(2):193-195,199
An efficient vacuum suction system is a necessary prerequisite for the smooth operation of the oral diagnosis and treatment.During the use of the dental units,there is often a situation of vacuum suction weakness,resulting in the inability to discharge the mixture of blood,saliva,dental tissue and other mixtures in time,which affects the doctor's treatment field and increases the risk of aspiration pneumonia and cross-infection in patients.The working principle,pipeline system,filters and other aspects of the vacuum suction system that may affect the suction efficiency was analyzed.The causes and solutions of vacuum suction weakness were discussed,and operation suggestions were proposed to ensure the safe and effective use of equipment and ensure the safety of diagnosis and treatment.
6.Risk factors for liver cancer in chronic hepatitis B patients and construction of a nomogram prediction model
Yichen ZHU ; Chunxia SHA ; Chunsun FAN ; Tiejun ZHANG
Journal of Clinical Hepatology 2024;40(12):2441-2449
ObjectiveTo investigate the risk factors for liver cancer in patients with chronic hepatitis B (CHB) in the Qidong Chronic Hepatitis B cohort, and to construct a nomogram model for predicting the risk of liver cancer in CHB patients. MethodsA structured questionnaire survey was conducted among the CHB patients, aged ≥18 years, who attended the outpatient service of Qidong Third People’s Hospital from January 1 to December 31, 2016. The onset of liver cancer was defined as the primary outcome, and the outcomes of the cohort were obtained from Qidong Cancer Registry. Baseline clinical features were compared ;between the liver cancer group and the non-liver cancer group. The independent-samples t test or the Mann-Whitney U test was used for comparison of continuous data between two groups, and the chi-square test or the Fisher’s exact test was used for comparison of categorical data between groups. The Cox regression model was used to analyze the risk factors for liver cancer in CHB patients and calculate their hazard ratio (HR) and 95% confidence interval (CI); the variables with statistical significance in the univariate Cox regression analysis were included in the LASSO regression analysis, and then the variables obtained were included in the multivariate Cox regression analysis to establish a predictive model. The nomogram was used to visualize the complex model. The receiver operating characteristic (ROC) curve, index of concordance (C-index), and the calibration curve were used to assess the predictive efficacy of the model, and the decision curve was used to evaluate the clinical practicability of the nomogram. ResultsA total of 1 479 CHB patients were selected, among whom 58 patients with a confirmed diagnosis of liver cancer, 15 with missing data on testing indicators, and 164 with missing data on important information in the questionnaire were excluded, and finally 1 242 subjects were included in the study. Up to December 31, 2023, there were 67 new cases of liver cancer after a median follow-up time of 7.71 years, and the incidence density of liver cancer was 729.78/100,000 person-years. There were significant differences between the liver cancer group and the non-liver cancer group in age, sex, educational level, liver cirrhosis, duration of liver cirrhosis, history of diabetes mellitus, albumin, total bilirubin (TBil), direct bilirubin, aspartate aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase (GGT), and alkaline phosphatase (all P<0.05). The multivariate Cox regression analysis showed that the increase in age (HR=1.07, 95%CI: 1.05 — 1.10, P<0.001), a relatively high level of TBil (HR=1.98, 95%CI: 1.15 — 3.42, P=0.014), a relatively high level of GGT (HR=2.41, 95%CI: 1.43 — 4.08, P=0.001), and a long duration of liver cirrhosis (HR=1.09, 95%CI: 1.02 — 1.15, P=0.009) were independent risk factors for liver cancer in CHB patients. A nomogram prediction model was constructed based on the above four indicators, with an area under the ROC curve of 0.790, 0.845, and 0.829, respectively, in predicting the risk of liver cancer in CHB patients at 1, 3, and 5 years, and the bootstrap resampling method was used for internal validation and showed a C-index of 0. 778. The calibration curve showed that the prediction model had good stability, and the decision curve showed that it had certain clinical practicability. ConclusionThe increase in age, relatively high levels of TBil and GGT, and a long duration of liver cirrhosis are independent risk factors for liver cancer in CHB patients, and the nomogram model constructed based on these factors has a good predictive value and can be used in clinical practice to help develop strategies for the long-term monitoring of liver cancer.
7.Hedgehog signaling in gastrointestinal carcinogenesis and the gastrointestinal tumor microenvironment.
Jinghui ZHANG ; Jiajun FAN ; Xian ZENG ; Mingming NIE ; Jingyun LUAN ; Yichen WANG ; Dianwen JU ; Kai YIN
Acta Pharmaceutica Sinica B 2021;11(3):609-620
The Hedgehog (HH) signaling pathway plays important roles in gastrointestinal carcinogenesis and the gastrointestinal tumor microenvironment (TME). Aberrant HH signaling activation may accelerate the growth of gastrointestinal tumors and lead to tumor immune tolerance and drug resistance. The interaction between HH signaling and the TME is intimately involved in these processes, for example, tumor growth, tumor immune tolerance, inflammation, and drug resistance. Evidence indicates that inflammatory factors in the TME, such as interleukin 6 (IL-6) and interferon-