Objective To investigate the expression and clinical significance of BRMS1 ( breast cancer metastasis suppressorl ) protein in colon cancer.Methods The expression of BRMS1 protein was detected by using EliVision immunohistochemical techniques in 46 cases of colon cancer,and adjacent non cancerous colon tissues.The clinical significance with histopathologic records was aralyzed.Results The expression levels of BRMS1 ( 34.8％ ) in the colon cancer tissues was significantly lower than those of adjacent non cancerous colon tissues( x2 =23.92,P ＜0.01 ).The expression of BRMSl was significantly correlated with tumor size,clinical stage,and lymph node status (x2 =6.02,4.28,4.35,all P＜0.01) ;BRMS1 had no correlation with age,pathological type.Conclusion BRMS1 might synergistically promote the metastasis of colon cancer.Detection of the expression of BRMS1 may be hdpful in determineing the prognosis of colon cancer.

Objective To study the effects of UVA and tea polyphenols(TPP,a kind of polyphenol substances from a special plant) on lipid peroxidation and cell kinetics in primarily cultured rat skin keratinocytes.Methods The release of cytoplasm enzyme, lactate dehydrogenase( LDH), lipid peroxidation product, malondialdehyde (MDA) ,and anti-peroxidant, glutathione peroxidase(GSH-Px) ,were assayed in primary cultured keratinocytes treated by UVA and TPP. Cytometry and Flow Cytometer were used to count the viable cells and the cell kinetics,too. Results UVA could obviously improve the release of LDH from 827.55 U/L to 1 312.47 U/L,increase the formation of peroxidation product MDA,decrease the level of GSH-Px and reduce the survival rate of cells and the cell proliferative index( PI) from 34.24% to 17.98 %. TPP could significantly protect and inhibit the damage induced by UVA. Conclusion UVA could damage skin keratinocytes, and TPP could protect keratinocytes from damage induced by UVA. The result could provide useful information in developing natural anti-violet product.

Objective To study the effects of UVA and tea polyphenols(TPP,a kind of polyphenol substances from a special plant) on lipid peroxidation and cell kinetics in primarily cultured rat skin keratinocytes.Methods The release of cytoplasm enzyme, lactate dehydrogenase( LDH), lipid peroxidation product, malondialdehyde (MDA) ,and anti-peroxidant, glutathione peroxidase(GSH-Px) ,were assayed in primary cultured keratinocytes treated by UVA and TPP. Cytometry and Flow Cytometer were used to count the viable cells and the cell kinetics,too. Results UVA could obviously improve the release of LDH from 827.55 U/L to 1 312.47 U/L,increase the formation of peroxidation product MDA,decrease the level of GSH-Px and reduce the survival rate of cells and the cell proliferative index( PI) from 34.24% to 17.98 %. TPP could significantly protect and inhibit the damage induced by UVA. Conclusion UVA could damage skin keratinocytes, and TPP could protect keratinocytes from damage induced by UVA. The result could provide useful information in developing natural anti-violet product.

ObjectiveTo investigate the radioactive level of gross alpha and beta in drinking water in one district in Shanghai and provide a scientific basis for measuring the level of radiation in case of drinking water pollution due to potential nuclear accidents. MethodsA total of154 samples collected across the district were monitored by using the standard examination method for drinking water - radiological parameters GB/T 5750.13-2006. ResultsAll the samples of the drinking water conformed to the standard for drinking water quality GB 5749-2006. The differences between different seasons were significant. The difference of gross alpha and beta radioactivity of drinking water was significant between the high water period and the dry water period. The former was higher than the latter. ConclusionIt is very important to monitor and study radioactivity of drinking water regularly for the prevention and control of health damage caused by radioactive water pollution.