This paper reported the estimation of free calcium concentration in rabbit resting lymphocytes, and the effect of (-) Daurisoline (8701) on the cyto-plasmic free calcium concentration by using fluorescent indicator Fura -2/Am estimating technique. Results were compared with the effect of the Ca2-ionophore A23187.The results indicated that the free calcium concentration of resting lymphocytes was 109. 6nmol ? L-1, after 10-5 mol? L-1,10-4.5 mol ? L-1,and 10-4 mol ? L-1-)Daurisoline administration, the cyto-plasmic free calcium concentration increased to 178. 8%, 204. 4%and 817. 5% respectively, while same concentration A23187 only led the increase of 164. 6% , 198.4% and 327% respectively.Results suggested that ( - )Daurisoline may be a Ca2- agonist and may induce the cytotoxicity effect on lymphocytes at high concentr ation.

OBJECTIVE To sdudy the characteristics of nosocomial infections in psychiatric hospital and to take the corresponding prevention measures to control. METHODS The epidemiological characteristics of nosocomial infections occurred in the patients treated in psychiatry department of our hospital from Jan 2003 to Dec 2006 were retrospectively analyzed. RESULTS Of all 5741 patients, 367 cases were definitely diagnosed as nosocomial infection. The incidence was 6.39%, with a decreasing trend. Among these infections, upper respiratory tract infections accounted for 52.78%, lower respiratory tract infections for 13.26%, and gastrointestinal infections for 11.14%. Other sites infections were rare(11.41%). The respiratory tract infection occurred all the year around, especially in the winter, other sites (included skin) infections mainly occurred in the first and the third quarters. The gastrointestinal infection reached its highest in the summer. CONCLUSIONS The nosocomial infection in psychiatric hospital has some characteristics and regularities. These are related to the particularity of the patients, the long stay in hospital, and poor self-care ability. Accordingly, we can take reasonable measures to prevent and control nosocomial infection.

ObjectiveTo study the characteristics of the relationship between injury and disease in foren-sic identification cases of cervical trauma with cervical vertebra degeneration, and to explore the prob-lems about how to identify the participation rates of injury and disease using the clinical information, forensic examination and imaging examination.MethodsSeventeen forensic identification cases of cervi-cal trauma with cervical vertebra degeneration were collected. The age distributions, injury formations, injury severities and imaging findings of these cases were analyzed and the relationship between injury and disease was evaluated comprehensively.ResultsMiddle-aged and elderly were common in 17 cases and every case was involved with intervertebral disc herniation. The main reasons of injuries were hy-perextension. The degree of injury severity and vertebra degeneration were graded according to the imag-ing findings. The participation rates of injury and disease were also calculated comprehensively.Conclu-sionThe forensic identification cases of cervical trauma with cervical vertebra degeneration should be evaluated with clinical information, forensic examination and imaging finding.

The biomarkers can facilitate the evaluation of the factors modulating susceptibility to carcinogens among human populations. As an indicator of DNA damage, carcinogen-DNA adducts represent a critical step on the carcinogenic pathway. Genetic differences in detoxification capabilities and the DNA repair efficiency may modulate PAH-induced DNA damage and carcinogenesis. Experimental animal studies have demonstrated that the fetus and infant are more susceptible to polycyclic aromatic hydrocarbons (PAHs) induced carcinogenesis than adults. Recent years, more and more studies have shown that the prenatal exposure to PAHs not only associated with the increasing risk of cancer, but also related to adverse birth outcomes. This paper reviewed the differential susceptibility to PAHs between the paired maternal and newborn and discussed the relationship between the DNA adducts levels and the gene polymorphisms.

Staphylococcus aureus is a Gram-positive bacterium with strong pathogenicity. With the widespread use of antibiotics， its multi-drug resistance has gradually increased. Among them， methicillin-resistant S. aureus （MRSA） is one of the main pathogens of hospital and community infections. Antimicrobial peptides are short-chain peptides with good antibacterial effects and low drug resistance， which have been widely studied in recent years. This study summarizes the mechanism of action of antimicrobial peptides and related study on antimicrobial peptides against MRSA from different sources. It is found that the mechanisms of action of antimicrobial peptides include targeting bacterial cell membranes， bacterial cells， and bacterial cell walls， etc. Besides isolating antimicrobial peptides with anti-MRSA activity from animals， plants， and microorganisms， antimicrobial peptides can also be obtained through synthetic methods. Among them， GHa-derived peptides from animal sources， Ib-AMP4 from plant sources， Ph-SA from microbial sources， the synthetic peptide LLKLLLKLL-NH2， and so on， due to their effective antibacterial activity， rapid bactericidal speed， and low toxicity， are promising candidates for anti-MRSA drugs.

Objective To explore the impacts of high temperature on health in summer in different cities of China.Methods The ettects of high temperature on health in different cities were analyzed through metereological data collecting and questionnaire survey on health of residents in Guangazhou,Nanjing and Harbin cities,300 cases respectively.Results Guangzhou,Nanjing and Harbin locat in different latitudes and have deifferent climates.Guangzhou had the high temperature all the year,with little daily temperature difference.Nanjing had cold winter and hot summer.Harbin's temperature all the year round and large daily temperature difference.In summer of 2003,the utilization rate of air-condition was 85%,75% and 12%,(respectively in Guangzhou,Nanjing and Harbin),the insomnia rate was 21.6%,38.3% and 12.9%,the occurrence rate of fatigue was 21%,25.1% and 15.2%,the occurrence rate of aggravation of existing diseases was 5.0%,7.3% and 6.1%,the incidence rate of heatstroke was 21.6%,38.3% and 12.9%,respectively.Conclusion High temperature in summer can lead to not only heat stroke,but also insomnia,fatigue,aggravation of existing diseases,et al.The residents in different cities have different tolerance to high temperature.The continual climate warming will threat human health in different latitude cities in difterent latitudes.

In order to obtain some evidences of the role of parathyroid hormone (PTH) in regulating calcium metabolism, and explore the mechanisms by which PTH regulates calcium in the heart, 45Ca was used to study the influence of bovine PTH1-34 fragment (bPTH1-34) on calcium transsacolemma. The results indicate that bPTH1-34(l?I0-7mol ? L-1) increases beating rate of heart cells under normal state and it markedly increases the rapid (5 min) and the slow (120 min) phases of 45Ca influx in heartcells (under normal, KC1 10-5 mol?L-1 high K+depolarised, NA 10-7mol?L-1receptors activated states), but it could also reduce 45Ca efflux from the cultured heart cells. It suggests that the mechanisms caused by bPTH1-34 which enhances the cytosolic calcium concentration and myocardial beating rate may be related with cAMP level elevated in heart cells.

Totally, 125 elderly inpatients with aspiration pneumonia (AP) were enrolled from departments of respiratory medicine and neurology at Zhongnan Hospital, Wuhan during June 2005 to October 2009 for analysis. Results showed that listlessness was manifested in 70 cases (56%), primary neurological illness in 80 cases (64%), 57 of them (46%) complicated with respiratory failure and 26 died with case-fatality of 21%. Main pathogen for them was Gram-negative bacillus, accounting for 57% of the total. Two kinds or more of antibiotics had ever been administered in 98 cases (78%), with an average length of antibiotics use for 28 days, ranging from one day to 128 days. Nasal feeding was instituted for those with choking over their feeding and coughing, and their inspired feeding was aspirated from the trachea or bronchi and lavished with bronchoscopy, with 38 cases by tracheal intubation, 26 by tracheotomy and 42 with artificial ventilation. Their average duration of hospital stay spanned 28 days with an average cost of 25 000 RMB yuan per capita, significantly higher than that for those with common pneumonia. In conclusion, clinical features of these elderly patients with AP were not so typical, most of them manifest severe, with lots of medical cost and bad prognosis.

Objective To discuss the relationship between chronic bronchitis and meteorological conditions in Nanjing. Methods Took the record of the recrudescing time of 200 old chronic bronchitis patients for a year. The correlation between the changing of air temperature and pressure and the monthly chronic bronchitis recrudescing in a year was analyzed. The main meteorological conditions by which recrudescing were affected in different season were selected with multiple stepwise correlation analysis. Results There were chronic bronchitis patients recrudescing the whole year, but the most were in winter and the least in summer. The most recrudescing happened in December and April, and the least in May and July. The meteorological factors that affected chronic bronchitis patients recrudescing were air temperature, pressure and wind speed. The relative coefficient of case numbers between 24 hours variations of air temperature and pressure was 0.5762(P=0.0499) and 0.5841(P=0.0461), respectively. Conclusion The steady meteorological conditions are not good for recrudescing of chronic bronchitis patients. And temperature descent and high air pressure are the main meteorological factors that induce chronic bronchitis recrudescing.

Design otolaryngology chair’s up-down assemble by applying link mechanism. And analysing the speed and accelerate of mechanism in CATIA’s motion mechanism module to verify its actural running status. Draw the effective design, make the successful prototype.