Objective To explore the clinical effect of different scheme of moxifloxacin and levofloxacin for elderly multi drug resistant pulmonary tuberculosis.Methods 136 cases of elderly patients included in the study were randomly divided into the observation group 1 and observation group 2 with 68 cases in each group according to the sequence in group.The observation group 1 used moxifloxacin regimen for treatment of pulmonary tuberculosis while the observation group 2 used levofloxacin regimen.The two groups were treated for 18 months and observed the clinical curative effect,and the sputum negative,pulmonary lesions absorption,empty changes and adverse reactions.Results The total efficiency of the observation group 1 was 92.6％,which in the observation group 2 was 72.1％,the total efficiency of the two groups had a significant difference (x2 =9.917,P =0.002) ;the sputum negative conversion rate of the observation group 1 at the end of 3 months was significantly higher than that of the observation group 2 (x2 =4.115,P =0.043),no significant difference was found at the other time points (all P ＞ 0.05) ; after treatment,the obvious absorption + absorption of lung lesion in the observation group 1 was 59 cases,accounting for 86.8％,that in observation group 2 was 48 cases,accounting for 70.6％,two groups of lung lesions absorption had significant difference (x2 =5.303,P =0.021) ; the pulmonary cavity closure + reduced in the observation group 1 was 52 cases,accounting for 76.4％,that in the observation group 2 was 41 cases,accounting for 60.3％,with significant difference between the two groups (x2 =4.115,P =0.043) ;there was no significant difference in adverse reaction of the two groups (P ＞ 0.05).Conclusion Moxifloxacin anti tuberculosis regimen has a better curative effect in treatment of elderly patients with multi drug resistant pulmonary tuberculosis,which should be expanded the application.

OBJECTIVE:To establish the quality method control of Jiufen spray,a percutaneous administration preparatio_n.METHODS:To determine the contents with TLCs method.The thin-layer plate was made of silica gel GF254. The spread out agent was chloroform-methanol-ammonia solution(4mol/L)(2∶6∶1). Dual wavelength scanning:strychnine and brucine:?s=254nm,?r=325nm;ephedrine:?s=500nm,?r=440nm.RESULTS:The within-day recoveries of strychnine,brucine and ephedrine were 99.048?2.388,99.532?0.958 and 99.504?0.555;the between-day recoveries were 98.796?1.058,99.58?0.476 and 99.42?0.838;the contents were(1.474?0.047)%,(1.372?0.052)%and(1.506?0.064)%(n=4) respectively.CONCLUSION:This method is stable and reliable and can be used for determination of the contents of this preparation.

Objective To summarize the perioperative nursing of male patients with breast cancer.Methods Retrospectively, the clinical data of 9 male patients with breast cancer undergoing radical mastectomy from January 1990 to July 2011 were analyzed to summarize the perioperative nursing strategies.Result The radical mastectomy for the 9 patients was successful.2 patients contracted complications as hemorrhage in 1 case and flap necrosis in 1 case.Conclusion The perioperative nursing should be performed based on the characteristics of male patients with breast cancer.

Objective: To screen the receiving solution used to study the transdermal absorption of strychnine in Jiufen spray. Methods: With recovery,Q-T equation and transdermal speed constant as the parameters, the improved Franz-cell and the skin of SD rats were used to screen the receiving solution from saline, saline-alcohol (7∶3,v∶v), pH phosphate buffer solution-alcohol (88∶2,v∶v), pH 7.4 phosphate buffer containing 25% alcohol. Results: The comparison of the inter-day and intra-day recoveries,Q-T equation and transdermal speed constant showed that saline-alcohol(7∶3) was the best for the study. Conclusion: It is indicated that saline-alcohol(7∶3) was the best receiving solution for the transdermal absorption study of Jiufen spray.

0.05 ), However, all pasameters showed significantly milder or lower in interferon ? treated group than incontrol group at the 6th, 12th week( P

Object To screen the prescriptions of JIUFEN SPRAY Methods Four prescriptions were primarily obtained from 19 prescriptions of JIUFEN SPRAY on the basis of their stability and spraying effect, then the obtained four prescriptions were screened further according to transdermal rate of them Results Prescription 17th was considered to be the best as its high stability, spraying effect and transdermal rate Conclusion The optimal prescription of JIUFEN SPRAY consisted of 20% alcohol solution of sample, 3% borneol and 5% glycerine

Objective: To study the transdermal releasing rule of the preparation of Jiufen Spray.Methods: Improved Franz diffuser was applied to transdermal experiment with TLC scanning method.Results: Q T (quantum time) equations of Jiufen Spray are strychnine:Q=220.941t- 486.006 , brucine:Q=208.146t-454.629, ephedrine:Q=177.691t-247.826. The study on releasing rule suggests the accumulative amount of transdermal drug increases with time, but the releasing speed is roughly stable, the total releasing ratio of 12 hours is about 50%. Conclusion: Jiufen spray could surmise to maintain relatively stable plasma drug concentration.

Objective To observe whether electroacupuncture (EA) is protective to the blood-brain barrier (BBB) in rabbits with acute cerebral infarction and explore the underlying mechanisms. Methods Eighty rabbits were used in this study. After establishing the acute cerebral infarction rabbit model,all the animals were randomly allocated to a model group and an EA group. The acupoint "Bai Hui" and "Shui Gou" in Du meridian in the rabbits of the experimental group were stimulated by EA. The content of ANP,cAMP and cGMP was examined. Results Compared with the model group,the content of ANP in blood plasma and cAMP in brain tissue of the EA group significantly increased,while the content of cGMP decreased,the ratio cAMP/cGMP increased nearly to the normal level. Conclusion The EA exerted protective effect and promoted repair of BBB in rabbits with acute cerebral infarction,which might be modulated by the changes of the content of ANP in blood plasma and cAMP,cGMP in brain tissue.

Objective To evaluate clinical effect of accurate pedicle subtraction osteotomy (PSO) using osteotomes in the treatment of thoracolumbar kyphosis (TLK).Methods From June 2007 to October 2010,18 patients with TLK underwent accurate PSO using osteotomes under X-ray fluoroscopy,including 13 males and 5 females,with an average age of 48.6 years.The primary causes of TLK included old fracture (11cases),chronic tuberculosis (4 cases) and hemivertebra (3 cases).Deformity apex occurred at T12 (5 cases),L1 (9 cases),and L2 (4 cases).Radiological assessment for sagittal balance was performed by measuring Cobb angle.The Frankel grade,visual analogue scale (VAS) and Oswestry disability index (ODI) were used to evaluate pre-and post-operative neurological status,back pain and function.Results The mean operative time,mean blood loss and mean postoperative drainage volume were 247.0±29.3 minutes,708.5±34.5 ml and 337.3±74.6 ml,respectively.All patients were followed up for 1 to 4.5 years (average,2.8 years).Solid fusion was achieved in all patients.Cobb angle was corrected from preoperative 42.3°±5.7° to 2.2°±1.9° three months postoperatively and 2.7°±2.1 ° at final follow-up.VAS and ODI scores decreased from preoperative 8.5±1.0 and 72.8％±8.3％ to 2.1±0.7 and 21.6％±9.2％ three months postoperatively,and 1.9±0.6 and 19.3％±8.6％ at final follow-up,respectively.With regard to Frankel grade,a 1-grade and 2-grade improvement was observed in 7 cases and 2 cases 3 months postoperatively,respectively.At final follow-up,a 1-grade and 2-grade improvement was observed in 5 cases and 4 cases,respectively.Two patients had transient neurological symptoms postoperatively,which recovered after drug treatment for 2 weeks.No other complications occurred.Conclusion It is safe and effective to correct TLK through accurate PSO using osteotomes,which has some advantages,such as less blood loss,higher fusion rate and fewer complications.

Objective To develop a novel padlock probe and rolling circle amplification(RCA) to detect 5 common cell culture contaminant Mollicute( Mycoplasma arginini, Mycoplasma fermentans, Mycoplasma hyorhinis, Mycomplasma orale, and Acholeplasma laidlawii ). Methods "Universal" primers ( SPS1, SPA2 and SPS2, SPA1) were used to amplify the Mollicute 16S-23S rRNA intergenic spacer region.Amplicon was ligate Mollicute specific padlock probe. Probe amplified and monitored using a Corbett RotorGeneTM 6000 machine. Results Five reference Mollicute strains were correctly detected by RCA method.There was no cross-reaction. RCA method can sensitive detect 10 copies templates and show strong positive signal. Sixty-two cell culture specimens were detected. Thirty-seven samples were contained single specie Mollicute and 14 samples contained two Mollicutes. Eleven samples did not contained Mollicute. RCA detection results were concordant with previously species-specific PCR. Conclusion RCA can rapid, sensitive and specific detect contamination Mollicute.