Objective:To observe efficacy of Weichangshu in treatment of diabetic gastroparesis(DGP).Methods:96 cases of DGP conformed with the criteria were randomly divided into two groups.They were treated by oral administration of Weichangshu and Mosapride respectively for 4 weeks in addition to original treatment program for controlling blood sugar.Changes of clinical symptoms,FBG and P2hBG,gastric antrum-duodenum interdigestive period migrating motion compound wave(MMC)and electrogastrogram(EGG)before and after treatment were investigated.Results:Clinical symptoms were significantly improved in both groups.The markedly effective rate was 62.50% and the total effective rate was 91.67% in the Weichangshu group,and the corresponding index was 60.42% and 89.58% in the Mosapride group.After treatment,MMC significantly improved in both groups,with significant differences in lasting time of phase Ⅱ,and phase Ⅲ,and the contraction amplitude as compared with those before treatment(P

[Objective] To evaluate the therapeutic effect of Oleum Curcumae Aromaticae (OCA) on leukemia cell strain HL-60 and K562 by observing its sensitivity in inhibiting HL-60 and K562. [Methods] Subculture of HL-60 and K562 cells was performed on the 96-hole culture plate and OCA 100?L at the final concentration of 400, 200, 100 and 50mg/L respectively was added. The blank control was added with RPMI-1640 culture fluid. After 24, 48 and 72 hours of treatment, MTF 10?L (5mg/mL) was added and the inhibitory rate on the cells was assayed by detecting the absorption value at 570nm wavelength. [Results] The inhibitory effect of OCA on HL-60 and K562 increased with the treatment time. The difference of inhibitory rates 24, 48 and 72 hours after treatment were significant (P

Adult ; Aged ; Drugs, Chinese Herbal ; therapeutic use ; Dyspepsia ; drug therapy ; Female ; Gastric Emptying ; drug effects ; Gastrointestinal Agents ; therapeutic use ; Gastrointestinal Motility ; drug effects ; Humans ; Male ; Middle Aged ; Myoelectric Complex, Migrating ; drug effects ; physiology ; Phytotherapy

To observe the therapeutic efficacy of acupuncture plus three-step analgesic ladder for cancer pain relief.Method:Thirty-siX cases with cancer pain were randomized into a treatment group and a control group.Twenty-four cases in the treatment group were treated with acupuncture plus three-step analgesic ladder approach and 12 cases in the control group were treated with three-step analgesic ladder approach alone.Result:The total effective rate and marked effect rate of the treatment group were 95.8%and 54.2% respectively,while those of the control group were 83.3%and 25.0%respectively,indicating a significant statistical difference (P＜0.05) in terms of the therapeutic efficacy of the two groups and acupuncture plus three-step analgesic ladder approach can further improve the therapeutic efficacy.

BACKGROUND: Renin-angiotensin-aldosterone system existed in bone tissue. Recent studies on antihypertensive drugs found that angiotensin converting enzyme inhibitor type antihypertensive drug was possibly effective for osteoporosis. Perindopril is one of the commonly used antihypertensive drugs. Whether perindopril affected bone metabolism or could be used in anti-osteoporosis has not been reported.
OBJECTIVE: To observe effects of perindopril on bone metabolism in a rat model of osteoporosis induced by retinoic acid.
METHODS: Fifty Sprague-Dawley rats were randomly divided into five groups, with ten in each group. In the model group and each perindopril groups, rats were intragastricaly administered retinoic acid solution 80 mg/kg per day. After successful model establishment, rats in different perindopril groups were intragastrical y administered perindopril 2, 4 and 8 mg/kg per day, once a day, for 42 consecutive days. In the normal control and model groups, rats were given an equal volume of distil ed water. Serum calcium, phosphorus, alkaline phosphatase, bone mass and bone mineral density were detected in each group. Expression of bone specific alkaline phosphatase and tartrate-resistant acid phosphatase mRNA in bone tissue was determined.
RESULTS AND CONCLUSION: Compared with the model group, after treatment with perindopril, serum calcium and phosphorus levels were increased, alkaline phosphatase activities were significantly decreased, bone mass and bone mineral density were obviously increased in rats with retinoic acid-induced osteoporosis. Expression of bone specific alkaline phosphatase mRNA was higher in the perindopril 8 mg/kg group than in the perindopril 2 and 4 mg/kg groups and model group. Tartrate-resistant acid phosphatase mRNA expression was higher in the perindopril 8 mg/kg group than in the model group. These results indicated that perindopril could improve partial bone metabolic biochemical markers in osteoporosis rats, promoted bone formation by up-regulating bone specific alkaline phosphatase mRNA expression, and had a certain preventive effect on retinoic acid-induced osteoporosis.

Objective To evaluate the dissection and hemostasis effects of harmonic scalpel in laparoscopic surgery. Methods The effect in patients underwent laparoscopic surgery with harmonic scalpel or with high frequency electricity scalpel was compared. Results All the operations were done by laparoscopy.No complications or massive blood loss occurred during and after operation.The average operation time ,blood loss of operation with harmonic scalpel were less than that with high frequency electricity scalpel. Conclusions Harmonic scalpel has precise cutting , controlled coagulation, and less tissue injury,it can increase the safety and microsurgery techinque of laparoscopic surgery.It is a very important equipmeat and useful for laparoscopic surgery .

Joint contracture is currently one of the very common diseases in rehabilitation medicine department.Once it occurs,it likely leads to such functional disturbances in the patients as restricted joint activities,decreased muscle strength around a joint and abnormal gait,seriously affecting the quality of life of the patients.Studies on its pathogenesis can help doctors better understand and treat the disease.In this paper,pathogenesis of the disease and progress in its therapy are summarized.

Objective To evaluate the effect of dexmedetomidine on the activity of glycogen synthase kinase-3 beta (GSK-3β) during propofol-induced apoptosis in hippocampal nerve cells of newborn rats.Methods Sixty male 7-day-old Sprague-Dawley rats,weighing 10-15 g,were divided into 6 groups (n=10 each) using a random number table:normal saline group (group NS),fat emulsion group (group F),propofol group (group P) and different doses of dexmedetomindine groups (group D25,group D50 and group D75).Normal saline and fat emulsion 100 μl were injected intraperitoneally in group NS and group F,respectively.In group P,propofol 50 mg/kg was intraperitoneally injected,and an increment of propofol 50 mg/kg was added after righting reflex completely recovered,with the total amount of 100 mg/kg.In group D25,group D50 and group D75,dexmedetomidine 25,50 and 75 μg/kg were intraperitoneally injected,respectively,and 30 min later propofol 100 mg/kg was administered.At 2 h after emergence,the rats were sacrificed,their brains were removed for determination of apoptosis in hippocampal nerve cells (by TUNEL),and the hippocampi were isolated for detection of the expression of GSK-3β and phosphorylated GSK-3β (p-GSK-3β) by Western blot analysis.The apoptosis index (AI) and ratio of p-GSK-3β/GSK-3β were calculated.Results Compared with group NS,AI was significantly increased,the expression of p-GSK-3β was down-regulated,and the p-GSK-3β/GSK-3β ratio was decreased in P,D25,D50 and D75 groups (P＜0.05).Compared with group P,AI was significantly decreased,the expression of p-GSK-3β was up-regulated,and the p-GSK-3β/GSK-3β ratio was increased in D25,D50 and D75 groups (P＜0.05).Compared with group D25,AI was significantly decreased (P＜0.05),and no significant change was found in the expression of p-GSK-3β or ratio of p-GSK-3β/GSK-3β in D50 and D75 groups (P＞0.05).Compared with group D50,AI was significantly decreased (P＜0.05),and no significant change was found in the expression of p-GSK-3β or ratio of p-GSK-3β/GSK-3β in group D75 (P＞0.05).Conclusion The mechanism by which dexmedetomidine attenuates propofol-induced apoptosis in hippocampal nerve cells may be related to inhibition of GSK-3β activity in newborn rats.

OBJECTIVE: To discuss the potential problems caused by the exfoliation of the tunic albuginea after the low-temperature plasma operation. METHOD: To observe the occurrence of the tunic albuginea, exfolliating time, wound healing and its complications by following the patients received the low-temperature plasma operation from November in 2012 through June in 2013 (including 116 cases of tonsillectomy, 98 cases of adenoidectomy, 255 cases of the ablation of the inferior turbinate,8 cases of the ablation of the cyst of epiglottis, and 4 cases of the ablation of the tumor of larynx) together with reviewing the relevant literatures. RESULT: The tunic albuginea exfolliated about two weeks after tonsillectomy, bleeding was found in 3 cases, one of which was related with inappropriate eating; the exfoliation about two or three weeks after adenoidectomy, without the occurrence of the narrow nostril and pharyngeal cavity adhesion; about four weeks after the ablation of the cyst of epiglottis, bleeding was found in 1 case; about three or four weeks after the ablation of the inferior turbinate, massive bleeding was found in one case after two months; and the ablation of the tumor of larynx, one with severe atypical hyperplasia and carcinoma in situ, the theunic albuginea did not completely exfoliate four moths after the surgery and new creatures could be found, the wound looked smooth and healed well nine moths after the surgery. The full exfoliation occur four months after the surgery for one with the neurilemmoma in the left laryngeal ventricle and the new creatures could also be found. It exfoliated eighth days after the surgry for one patient with left subglottic epitheliaoid heman-gio endothelioma and it did not recur even six months after the surgery. Another one with the cyst of the left plica aryepiglottica failed to be followed. The first three patients did not suffer from such complications as bleeding, endogenous in trachea, etc. CONCLUSION Low-temperature plasma surgery has obvious advantages, but the potential problems caused by the surgery need to be taken in accounted, especially the exfoliating time of the tunia albuginea is different after the ablation of the tumor of larynx, which is inconsistent with what the literatures reported, it could partially due to the action time of low-temperature plasma surgery with surrounding tissues.
Adolescent ; Adult ; Catheter Ablation ; methods ; Child ; Child, Preschool ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Postoperative Complications ; Young Adult

OBJECTIVE: To analyze the results of hearing screening and the related factors in 3200 newborn infants, in order to know the incidence of neonatal hearing disorders. METHOD: Three thousand two hundred cases of newborn infants born during 2014-01 and 2015-03 were tested for hearing screening. According to risk factors, newborns were divided into the normal newborn group (2945 cases) and the high-risk neonatal group (255 cases). Using fast brainstem auditory evoked potential (AABR) to screen the hearing screening of newborn infants with 3~5 d; those who did not pass the screening, after the birth of 30~42 d re-screening; re-screening still not pass, within 3 months line diagnostic auditory brainstem evoked potential (diagnostic ABR) confirmed. Comparative analysis of newborn hearing screening by screening through rate, re-screening through rate and hearing impairment rate proportional relationship with the relevant factors, and the differences of hearing screening results in the normal newborn group and the high-risk neonatal group. RESULT: (1)The rate of neonatal hearing screening did not pass was 7.50% (240/3,200); 30 cases was not involved in re-screening, the rate of re-screening did not pass was 15.24% (32/210); hearing impairment rate is about 0.38%(12/3200), and the bearing impairment positive rate was 1.31% (42/3200). (2) There was no significant difference of different delivery mode, neonatal sex in newborn hearing screening through rate, re-screening through rate and hearing impairment rate (P > 0.05). Different gestational age, maternal age in newborn hearing screening through rate, re-screening through rate and hearing impairment rate, the difference was statistically significant (P < 0.05). (3) Screening through rate and re-screening through rate of the high-risk neonatal screening group were significantly lower than the normal newborn group (P < 0.05), and hearing impairment rate was higher than normal newborn group (P < 0.05). CONCLUSION Strengthen prenatal health care, to reduce abnormal childbirth conditions (preterm birth, neonatal asphyxia), universal newborn hearing screening and improve screening accuracy, and to strengthen newborns with risk factors for early diagnosis and intervention, to reduce the incidence of neonatal hearing impairment has important significance.
Evoked Potentials, Auditory, Brain Stem ; Gestational Age ; Hearing Disorders ; epidemiology ; Hearing Tests ; Humans ; Incidence ; Infant ; Infant, Newborn ; Neonatal Screening ; Risk Factors