OBJECTIVETo observe therapeutic effect of acupuncture on late sudden deafness of different types.

METHODSAccording to the principles of TCM syndrome differentiation treatment, 63 cases were treated with acupuncture, once very other day, 7 days constituting one course with a interval of 3 days between two courses. Changes of the hearing threshold, and the difference of therapeutic effects between different states of illness and different types were investigated.

RESULTSFour cases (6.3%) were cured, 17 cases (27.0%) were markedly effective, 19 cases (30.2%) were effective, and 23 cases (36.5%) were ineffective, the total effective rate being 63.5%; there was a significant difference in the mean hearing threshold before and after treatment (P < 0.05).

CONCLUSIONThe milder the state of illness, the better the therapeutic effect of the acupuncture; in addition, acupuncture has the best therapeutic effect for the type with decrease of the curve of audition, and better therapeutic effects for the types of upward invasion of phlegm-heat, and the type of stagnation of turbid phlegm in the middle-jiao.

Acupuncture Therapy ; Combined Modality Therapy ; Hearing Loss, Sudden ; therapy ; Humans ; Mucus ; Syndrome

Objective To explore the effects of beta-amyloid precursor protein (APP17) peptide on the changes in the expressions of phosphoinositide 3-kinase(PI3K), protein kinase B(PKB) and phosphorylation of cAMP response element binding protein (p-CREB) in the neurons of hippocampal gyms in rats after cardiopulmonary resuscitation. Method Twenty-one Wistar rats were randomly divided into three groups, namely the sham-operated control group, the resuscitation group and resuscitation with APP17 peptide-treated group. The rat model of asphyxial cardiac arrest was made by clamping the endotracheal tube and the standard external cardiopulmonary resuscitation ( CPR) was performed until the restoration of spontaneous circulation ( ROSC) observed.ROSC was defined by the appearance of normal QRS waves of electrocardiogram and mean artery pressure ( MAP)≥60 mmHg for more than 10 minutes. Rats of resuscitation group and control group received intravenous 0.9%NaCl, and the rats of the APP17 peptide group were treated with APP17 peptide(10μg·300 g~(-1), i. v.) after ROSC. Rats were sacrificed by decapitation after reperfusion 2 hours and then the cerebral hippocampal gyrus was immediately separated to detect PI3K, PKB and p-CREB by immunohistochemistry ( IHC) and Western-blot analysis. Statistical comparisons were made by one-way analysis of variance (ANOVA) . Results IHC showed that there was no significant difference in PDK positive cells between resuscitation group and control group (2.75 ±1.80 vs. 2.53 ± 1.53, P > 0.05) . The PDK obviously more increased in the APP17 peptide group than in resuscitation group(5.85 ± 2.83 vs. 2.75 ± 1.80, P < 0.01) .The counts of PKB and p-CREB positive cells were obviously lower in resuscitation group than those in control group (2.45 ± 1.36 vs. 5.22 ± 2.50, P < 0.05);(2.41 ± 1.11 vs. 8.31 ±3.02, P < 0.01 ). The PKB and p-CREB positive cells were significantly higher in the APP17 peptide group than in resuscitation group (9.66±4.32 vs. 2.45 ±1.36, P < 0.01);(14.18 ± 3.96 vs.2.41 ± 1.11, P <0.01). The PKB and p-CREB protein levels in resuscitation group were lower than those in the control group. Conclusions The expressions of PKB and p-CREB in neurons of hippocampus gyrus 2 hours ROSC, and APP17 peptide could restorer the expression of PDK, PKB and p- CREB and thereby protect the neurons of hippocampus gyrus from the injury of CPR.

AIM:To evaluate the efficacy of vitrectomy in combination with intravitreal dexamethasone and vancomycin Derfusion in the management of severe post-traumatic endophthalmitis.METHODS:30 eyes diagnosed as posttraumatic infectiouis endophthalmitis were analyzed retrospectivly from April 2004 to April 2006.All the patients underwent vitrecLomy in combination with intravitreal drugs perfusion and were followed up for 12 to 24 weeks.The visual acuity,traumatic causes and microorganisms culture were analyzed.RESULTS:There are significant reduction in inflammation at 3 months after surgery.Infectious symptoms were completely controlled in 97% of the Cases(29/30).Final visual acuity were improved in 93% of cases (28/30).Among traumatic causes,foreign body is the most common cause (57%).StaphylococCUS aureus is the commonest microorganism.CONCLUSION:Vitrectomy in combination with intravitreal dexamethasone and vancomydn peffusion iS the most effective method in the treatment of Severe posttraumatic endophthamitis.

OBJECTIVETo explore the mechanism of cranial needling in treatment of acute intracerebral hemorrhage.

METHODSForty-three cases were randomly divided into a cranial needling plus medication group (n=21) and a medication group (n=22). The cranial needling plus medication group were treated with needling skull suture 4 days after the attack, once each day, for 10 sessions, on the basis of routine neurological treatment measures. The medication group were treated with the routine neurological treatment measures. Changes of the scores of neural function defect and IIL-6 contents before and after treatment were compared.

RESULTSThe IL-6 contents before and after treatment were (11.29 +/- 8.63) pg/mL and (1.91 +/- 1.02) pg/mL in the cranial needling plus medication group, and (12.15 +/- 7.23) pg/mL and (3.61 +/- 2.71) pg/mL in the medication group, respectively, with very significant differences before and after treatment (P<0.01), and with a significant difference between the two groups after treatment (P<0.05). There were very significant differences in the score of neural function defect before and after treatment in the two groups (P<0.01), and there was a significant difference between the two groups after treatment (P<0.05).

CONCLUSIONOn the basis of medication, cranial needling can promote rapid decrease of serum IL-6 content and improve nervous function in the patient of acute cerebral hemorrhage.

Acupuncture Therapy ; methods ; Acute Disease ; Adult ; Aged ; Cerebral Hemorrhage ; blood ; therapy ; Combined Modality Therapy ; Female ; Humans ; Interleukin-6 ; blood ; Male ; Middle Aged ; Scalp

OBJECTIVETo investigate polymorphisms of killer cell immunoglobulin-like receptor gene (KIR) in renal transplant recipients from southern Zhejiang.

METHODSKIR genotypes were analyzed by PCR-SSP in 416 renal transplant recipients, and the genotype frequencies were compared with populations from Eastern China and worldwide.

RESULTSAll 16 known KIR genes were detected in the renal transplant recipients, and KIR2DL4, 3DL2-3, 3PD1 were found in all. As a pseudogene, 2DP1 has a high genotype frequency (99%). The frequencies of KIR2DL1, 2DL3, 3DL1, 2DS4 have ranged from 92.1% to 98.8%. Compared with 11 groups in Eastern China and other countries, the KIR2DL2 phenotype frequency was higher (34.6%) than those of Shanghai, Zhejiang and Jiangsu populations (P<0.05). Among 41 genotypes, three have not been reported previously. The most common genotype was AA1, with a frequency of 43.51%, which was significantly lower than those of Jiangsu and Northern Zhejiang.

CONCLUSIONRenal transplant recipients from southern Zhejiang share similar features with Eastern China Han population with regard to KIR polymorphisms, but also have unique frequencies for KIR genotypes.

Adolescent ; Adult ; Aged ; China ; Female ; Gene Frequency ; Genotype ; Humans ; Kidney Transplantation ; methods ; Male ; Middle Aged ; Polymorphism, Genetic ; Receptors, KIR ; genetics ; Young Adult