Objective:To establish a laser light scattering method for the determination of the particle size distribution of clostridium butyricum enterococcus triple viable powder and compare the results of the sieving method.Methods:The conditions of laser scattering method were as follows:the vibration sampling rate of 80%,the dispersion pressure of 0.05 MPa,the background and sample scan time of 15 s,the shading of 0.5%-5%,the refractive index of the particles of 1.55,the particle absorption rate of 0.01,and the injection volume of 0.1-0.2 g.The eigenvalues of the particle size distribution were determined,which were the particle size cumulative distribution map of 10%,50% and 90% of the particle size value and the volume average particle diameter D.Results:The RSDs of d(0.1),d(0.5) and d(0.9) were less than 5% in the methodology study.The results of laser light scattering method showed that the particle size of 93.3% samples was below 250 μm,that of 64.2% samples was below 150 μm,that of 51.4% samples was below 125 μm,and that of 31.3% samples was below 90 μm.The results of sieving method showed that the particle size of 96.6% was below 250 μm,that of 46.4% samples was below 150 μm,that of 23.5% samples was below 125 μm,and that of 1.4% samples was below 90 μm.Conclusion:Sieving method and laser light scattering method both can characterize the particle size distribution of the sample.The laser light scattering method is simple,accurate and producible,which is suitable for the particle size control of clostridium butyricum enterococcus triple viable powder.

Here are reported for the first time two newly established methods of semi-quantitative measurement of specific antigens by means of n microscope photometer in the tissures stained using non-labelled enzyme immunocyio-chemistry-the point and area scanning methods. We measured the content of vassoprcssin-like material in the vassopressinergic neurons of hypothalamus in the control and foot shocked rats with the two methods,respectively. The results prove them, to some extent, significant in studying the content of specific material in specific tissues (e.g. some nuclei), and even in single cells. In addition, a comparison between the two methods was conducted in this study.

Child ; Child Welfare ; Clinical Trials as Topic ; Female ; Humans

Biomedical Research ; methods ; standards ; Disease ; etiology ; Humans ; Risk Factors

Americas ; Education, Medical ; standards ; Education, Medical, Continuing ; Germany ; Humans ; Internationality ; Internship and Residency ; Pediatrics ; education

Objective To investigate the diagnostic value of 64-slice CT and MRI in detection of abnormal sacroiliac joint changes in patients with early stage of ankylosing spodylitis (AS).Methods The 64-slice CT and MRI imaging data of the sacroiliac joint early diseases(grade 0 ～ Ⅱ lesion) for the 66 patients with clinical confirmed were reviewed retrospectively,of which MRI contrast enhanced was performed in 6 patients.Results CT showed grade zero lesion 15 cases,grade Ⅰ lesion in 20 cases and grade Ⅱ lesion in 31 cases.MRI revealed bilateral articular bone marrow edema in 52 cases,articular cartilage erosive destruction in 48 cases,fat accumulation of bilateral articular bone in 36 cases,articular bone erosive destruction in 31 cases.Bilateral synovial membrane enhancement was showed in all patients who underwent contrast enhanced.Conclusions MRI can detect acute inflammatory changes which can not display by MSCT.Compared with MSCT,MRI is more useful in detection of abnormal sacroiliac joint changes in patients with early stage of AS.

Object A method was developed for the determination of fatty acid in Fructus Bruceae oil by precolumn derivation HPLC Methods Fatty acids were derivatized with p bromophenacylbromide as derivative and 18 crown 6 as catalyst The method used C 8 Kromasil C 8 (250 mm?4 mm, 5 ?m) column and isocratic acetonitrile water eluent and the internal standard was heptadecanoic acid The detection wavelenghth was 254 nm Column temperature was fixed at room temperature, and the flow rate was 1 3 mL/min Results The standard curves of linoleic, palmitic, oleic, and steraric acid are linear within the range of 0 022—0 330, 0 014—0 213, 0 028—0 416, and 0 012—0 177 ?g, respectively The four fatty acid recoveries are 99 2%, 97 2%, 101 8%, 97 8%, and the RSD are 1 2%, 1 5%, 0 4%, 2 3%, individuslly Conclusion Five fatty acids are separated within 30 minutes during a single run The present method is reliable and relatively simple for the determination of fatty acid in Fruetus Bruceae oil

Objective To compare the contents of Ferulic Acid and Polysaccharide in Angelica Sinensis oliv. with Lotiform Angelica sinensis because of the morphosis related to lateral bud of Angelica Sinensis. Method The contents of Polysaccharide was determined by Polysaccharide-Phenol-Sulfuric acid reaction system. The content of ferulic acid was determined by HPLC. Result There is no significantly difference in the contents of Ferulic Acid and Polysaccharide between Angelica sinensis and lotiform Angelica sinens. Conclusion The contents of Ferulic Acid and Polysaccharide between Angelica Sinensis oliv. and Lotiform Angelica sinensis has no significantly difference because of the morphosis related to lateral bud of Angelica Sinensis.

Humans ; Liver, Artificial ; Translational Medical Research

Breast Feeding ; Consumer Product Safety ; Humans ; Infant ; Infant Food ; Nutritional Physiological Phenomena ; Triazines ; toxicity