Adult ; Humans ; Male ; Middle Aged ; Mining ; Molybdenum ; Pulmonary Ventilation ; physiology ; Radiography, Thoracic ; Respiratory Function Tests ; Silicosis ; diagnostic imaging ; physiopathology

Adolescent ; Adult ; Combined Modality Therapy ; Female ; Hexanes ; poisoning ; Humans ; Male ; Medicine, Chinese Traditional ; Occupational Diseases ; therapy ; Treatment Outcome ; Young Adult

Asthma, Occupational ; therapy ; Drainage ; methods ; Humans ; Sputum

Alveolitis, Extrinsic Allergic ; Female ; Humans ; Librarians ; Middle Aged

Asthma, Occupational ; etiology ; pathology ; Humans ; Inflammation ; pathology ; Neutrophil Infiltration ; Neutrophils ; pathology

Anthrax ; Humans ; Occupational Diseases ; microbiology

Brucellosis ; Humans ; Occupational Diseases ; microbiology

Disaster Planning ; Humans ; Rescue Work

Objective To optimize methods of culturing smooth muscle progenitor cells(SPCs) from mononuclear cells(MNCs) of peripheral blood. Methods Human MNCs isolated from buffy coat were seeded on M-199 with bovine pituitary extraction.On the eighth day outgrowth cells were stimulated with platelet-derived growth factor-BB(PDGF-BB).Fifteen days later,immunofluorescence,Western blot or RT-PCR was used to analyzed the expression of smooth muscle cell specific ?-actin(?-SMA),smooth muscle myosin heavy chain(SM MHC),Calponin,CD34,Tie-2 and Flk-1,and fluorescence activated cell sorter was employed to examine ?-SMA positive cells ratio. Results The cells stimulated by PDGF-BB for 15 d were positive for ?-SMA,SM MHC,Calponin,CD34 and Flk-1,but negative for Tie-2.The ?-SMA positive cells ratio was(90.57?5.63)%,significantly different from that of the control(P

Disease Notification ; Humans ; Occupational Diseases ; diagnosis