2.In-stent thrombosis in a patient with left main stem stenosis and platelet disorder.
Yi-tong MA ; Ding HUANG ; Yi-ning YANG
Chinese Journal of Cardiology 2010;38(5):466-467
Aged
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Blood Platelet Disorders
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complications
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therapy
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Coronary Artery Disease
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complications
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therapy
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Humans
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Male
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Stents
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Thrombosis
;
etiology
3.Ala nasal leiomyoma misdiagnosed as nasal vestibular cyst: a case report.
Cui-ping SHE ; Fu-mei MA ; Yi-feng TONG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(2):154-154
Child, Preschool
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Cysts
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Diagnostic Errors
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Humans
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Leiomyoma
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diagnosis
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Male
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Nose Neoplasms
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diagnosis
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Vestibular Diseases
5.Clustering of cardiovascular risk factors in patients of premature stable coronary heart disease complicated with nonalcoholic fatty liver
Zhong CHEN ; Genshan MA ; Fangyi XIE ; Yi FENG ; Jiayi TONG ; Chengxing SHEN ; Jiahong WANG ; Xiaoli ZHANG
Chinese Journal of General Practitioners 2008;7(3):164-167
Objective To study the characteristics of clustering of cardiovascular risk factors in patients less than 50 years-old of premature stable coronary heart disease(PSCHD)complicated with nonalcoholic fatty liver(NAFL).Methods One hundred and six patients with documented PSCHD were recruited into this study and their clinical data,including biochemical parameters,high-sensitivity C-reactive protein(hsCRP),white blood cell(WBC)count,ete.,were analyzed based on whether they had NAFL by B-type ultrasound scanning and their homeostasis model assessment ratio(Homa-IR)by the criteria for metabolic syndrome formulated by the International Diabetes Federation.Results Thirty-two (30.1percent)of 106 patients of PSCHD complicated with NAFL,and 74(69.9 percent)without NAFL. As compared to patients without NAFL,patients with NAFL had higher fasting blood glucose(FBS),serum level of insulin(INS),total cholesterol(TC),triglyceride(TG),serum activity of alanine aminotransferase(ALT),hsCRP,WBC count,body mass index(BMI),Homa-IR,and higher proportion of those with abnormal blood glucose,hypertension.metabolic syndrome(MS)and carotid atherosclerosis (CA)(P<0.05),respectively.Bi-variate correlation analysis revealed that hsCRP positively correlated to BMI,TG,ALT and Homa IR(r=0.420,P=0.000;r=0.200,P=0.040;r=0.218,P=0.048:and r=0.546,P=0.000,respectively)and inversely correlated with serum level of high-density lipoprotein cholesterol(HDL-C)(r=-0.220,P=0.023).WBC count positively correlated with FBS(r=0.211,P=0.030).BMI,hsCRP,ALT,and proportions of hypertension,diabetes,MS,NAFL and CA in patients with Homa-IR above median were significantly higher than those in patients with that below median ( P<0.05,respectively).Conclusions More risk faetors for chronic inflammatory reaction,cardiovascular disease and insulin resistance were clustered more obviously in patients of PSCHD complicated with NAFL.
6.Chemical constituents from Callicarpa nudiflora and their cytotoxic activities.
Yan-Chun MA ; Min ZHANG ; Wen-Tong XU ; Shi-Xiu FENG ; Ming LEI ; Bo YI
China Journal of Chinese Materia Medica 2014;39(16):3094-3101
The chemical consitituents from cytotoxic fraction of the Callicarpa nudiflora extract were isolated and purified by a combination of HP-20 macroporous resin, silica gel and Sephadex LH-20 column chromatographies. The structures were elucidated on the basis of the spectroscopic data and comparison of their spectroscopic data with reported data. The cytotoxicity was evaluated by the MTT assay. The 50% and 70% EtOH elutions of EtOH-extract showed significant cytotoxic activities, leading to the isolation of twelve compounds, which were identified as luteoloside(1), lutedin-4'-O-β-D-glucoside(2), 6-hydroxyluteolin-7-O-β-glucoside(3), lutedin-7-O-neohesperidoside(4), rhoifolin (5), luteolin-7, 4'-di-O-glucoside (6), forsythoside B (7), acteoside (8), alyssonoside (9), catalpol(10), nudifloside(11), and leonuride(12). Compounds 3-6, 10 and 12 were isolated from this genus for the first time, and compound 9 was isolated from this plant for the first time. The cytotoxicity assay demonstrated that flavonoids 1-6, in various concentrations, showed monolithic proliferation inhibitory activities against Hela, A549 and MCF-7 cell lines. Compounds 3, 5 and iridoid glycoside 11 possessed higher cytotoxicacivities. In short, flavonoids are the main components of cytotoxic extract from C. nudiflora, while phenylethanoid glycosides are the predominant ingredient but inactive to cancer cell lines. In addition, the minor iridoid glycoside expressed weak cytotoxic activity.
Callicarpa
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chemistry
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Cell Proliferation
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drug effects
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Cytotoxins
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chemistry
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isolation & purification
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pharmacology
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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pharmacology
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Humans
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MCF-7 Cells
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Molecular Structure
7.Quality Evaluation of Lignum Dalbergiae Odoriferae Medicinal Materials
Yalian WANG ; Ping YAN ; Yi ZHOU ; Tong ZHAO ; Xinye MA ; Ruoting ZHAN
Journal of Guangzhou University of Traditional Chinese Medicine 2015;32(6):1087-1090,1146
Objective To evaluate the quality of medicinal materials of Lignum Dalbergiae Odoriferae in Chinese herbal medicine market. Methods Eighteen batches of commercial medicinal materials of Lignum Dalbergiae Odoriferae were identified and analyzed by macroscopical identification, thin layer chromatography (TLC), and volatile oil assay according to the Chinese Pharmacopoeia published in 2010. Visible spectrometry was used to determine the content of total flavonoids from the qualified samples. And the gas chromatography was applied to evaluate the content of nerolidol from volatile oils of the qualified samples. Results Only 33.3%of the samples met the standard of Chinese Pharmacopoeia. The total flavonoid content of the qualified samples was in the range of 21.6-29.0 mg/g. The content of nerolidol from volatile oils of the qualified samples was in the range of 294-574 mg/g. Conclusion At present, the quality of medicinal materials of Lignum Dalbergiae Odoriferae in Chinese herbal medicine market and in clinic varies greatly, and adulterants and inferior are common. The contents of chemical components in different batches of samples are significant different.
8.Bone Marrow Mesenchymal Stem Cells Ameliorate Lung Injury through Anti-inflammatory and Antibacterial Effect in COPD Mice
LIU HONG-MEI ; LIU YI-TONG ; ZHANG JING ; MA LI-JUN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2017;37(4):496-504
The anti-inflammatory and antibacterial mechanisms of bone marrow mesenchymal stem cells (MSCs) ameliorating lung injury in chronic obstructive pulmonary disease (COPD) mice induced by cigarette smoke and Haemophilus Parainfluenza (HPi) were studied.The experiment was divided into four groups in vivo:control group,COPD group,COPD+HPi group,and COPD+HPi+MSCs group.The indexes of emphysematous changes,inflammatory reaction and lung injury score,and antibacterial effects were evaluated in all groups.As compared with control group,emphysematous changes were significantly aggravated in COPD group,COPD+HPi group and COPD+HPi+MSCs group (P<0.01),the expression of necrosis factor-kappaB (NF-κB) signal pathway and proinflammatory cytokines in bronchoalveolar lavage fluid (BALF) were increased (P<0.01),and the phagocytic activity of alveolar macrophages was downregulated (P<0.01).As compared with COPD group,lung injury score,inflammatory cells and proinflammatory cytokines were significantly increased in the BALF of COPD+HPi group and COPD+HPi+MSCs group (P<0.01).As compared with COPD+HPi group,the expression of tumor necrosis factor-α stimulated protein/gene 6 (TSG-6) was increased,the NF-κB signal pathway was depressed,proinflammatory cytokine was significantly reduced,the anti-inflammatory cytokine IL-10 was increased,and lung injury score was significantly reduced in COPD+HPi+MSCs group.Meanwhile,the phagocytic activity of alveolar macrophages was significantly enhanced and bacterial counts in the lung were decreased.The results indicated cigarette smoke caused emphysematous changes in mice and the phagocytic activity of alveolar macrophages was decreased.The lung injury of acute exacerbation of COPD mice induced by cigarette smoke and HPi was alleviated through MSCs transplantation,which may be attributed to the fact that MSCs could promote macrophages into anti-inflammatory phenotype through secreting TSG-6,inhibit NF-κB signaling pathway,and reduce inflammatory response through reducing proinflammatory cytokines and promoting the expression of the anti-inflammatory cytokine.Simultaneously,MSCs could enhance phagocytic activity of macrophages and bacterial clearance.Meanwhile,we detected anti-inflammatory and antibacterial activity of macrophages regulated by MSCs in vitro.As compared with RAW264.7+HPi+CSE group,the expression of NF-κB p65,IL-1β,IL-6 and TNF-α was significantly reduced,and the phagocytic activity of macrophages was significantly increased in RAW264.7+HPi+CSE+MSCs group (P<0.01).The result indicated the macrophages co-cultured with MSCs may inhibit NF-κB signaling pathway and promote phagocytosis by paracrine mechanism.
9.Selection of Cd~(2+) Binding-peptides and Their Affinities for Heavy Metal Ions
Jing-Shuang HUANG ; Chun-Yan MA ; Xin TONG ; Zhuo-Lin YI ; Liu XU ; Can-Quan MAO ;
Microbiology 1992;0(05):-
Selection and affinities of Cd binding peptides were assayed by phage random dodecapeptide library and affinity chromatography. Two Cd binding peptides were obtained, it was found that the affinities of Cu~ 2+ ,Co~ 2+ ,Zn~ 2+ ,Ni~ 2+ for Cd binding peptides were higher than that of Cd~ 2+ and Cr~ 2+ after detection of the amplified Cd binding peptides displayed phages to different heavy metal-charged resins; the detoxification of E.coli to Ni~ 2+ and Cd~ 2+ was enhanced when infected by Cd binding peptide displayed phages as compared with those of the control. The interaction of Cd binding peptide displayed phages with heavy metals charged resins was also observed under microscope. The work would be of great value and consequences for the study of interaction between heavy metals and proteins(peptides), as well as thedetoxification and bioremediation of heavy metals.
10. Changes of new urinary biomarkers in children with Henoch-Schonlein purpura nephritis
Journal of Shanghai Jiaotong University(Medical Science) 2020;40(6):842-846
Objective • To evaluate the changes of urinary angiotensinogen (UAGT), fibroblast-specific protein-1 (FSP-1) and thrombin in the children with Henoch-Schonlein purpura nephritis (HSPN). Methods • Fourteen children with HSPN (HSPN group), 28 children with Henoch-Schonlein purpura (HSP) but without renal injury (HSP group) and 23 children with normal urinalysis (control group) were included in the study. Ten HSPN children before treatment (untreated group), 9 HSPN children after glucocorticoid (GC) pulse therapy (GC group) and 8 HSPN children after GC and cyclophosphamide (CTX) double pulse therapy (GC+CTX group) were also selected in the study. Clinical information and fresh morning urine samples were collected from all the children. UAGT, FSP-1 and thrombin in urine were measured by kits. Urine creatinine (Ucr) was also measured for correction. Results • UAGT/ Ucr and FSP-1 in HSPN group were significantly higher than those in HSP group and control group (P<0.05). Thrombin in HSPN group had no significant difference, compared with HSP group (P>0.05), but thrombin levels in HSPN group and HSP group were both significantly higher than that in control group (P<0.05). UAGT/Ucr in HSPN untreated group had no significant difference, compared with GC group, while it was significantly higher than that in GC+CTX group (P=0.000). FSP-1 in untreated group was significantly higher than that in GC group, but had no significant difference, compared with GC+CTX group. There was no significant difference in thrombin among the 3 HSPN groups. Conclusion • UAGT/Ucr, FSP-1 and thrombin all increase in the urine of HSPN children, and UAGT/Ucr and FSP-1 may reflect the treatment effect to some extent. [Key words]