Acupressure ; Child ; Child, Preschool ; Combined Modality Therapy ; Female ; Humans ; Male ; Moxibustion ; Nocturnal Enuresis ; therapy

Objective To study the degradation of Rhizoma Typhonii polysaccharide with sulfuric acid under the normal and ultrasonic condition. Methods The orthogonal test table L9(34) was used to optimize 4 factors including acid concentration, reaction time, reaction temperature and ultrasonic power, with the yield of the low-molecular-weight polysaccharide as the index. Results The optimal conditions of discomposing Rhizoma Typhonii polysaccharide with sulfuric acid were 10% sulfuric acid, 100 ℃ for 2 h under the normal condition,and the yield of low-molecular-weight polysaccharide was 36.17%. Under the ultrasonic condition, the optimal conditions were 150 W of the ultrasonic power, 5% sulfuric acid, 40 ℃ for 1 h, and the yield was 44.63%. Conclusion The degrading yield of the low-molecular-weight polysaccharide can be increased under ultrasonic condition with sulfuric acid.

Objective To study the current cases of Keshan disease aims at providing scientific and basic evidence for prevention and control of the disease. Methods In accordance with historical cases, in 2009, an investigation was conducted in the permanent resident population in seriously infected representative villages of landscapes and ways of living such as Xujialeng, Zhuping and some other adjacent villages in Wudu district, Longnan city. People in those areas were examined and carefully observed based on the scheme of Keshan disease surveillance. Then they were enquired about their medical history, given normal clinical examinations and electrocardiogram(ECG) examination, the suspected cases were chest X-rayed. At the same time, some samples of their hair of some children under 12 and adults were collected to be examined for the selenium content. Results Among the total number of 515 subjects, 20 were diagnosed with Keshan disease with a total detection rate of 3.9% (20/515), among whom 13 were potential patients with a detection rate of 2.5%( 13/515), 7 were chronic patients with a detection rate of 1.4%(7/515). In the total number of 515 ECG, 48 were found with abnormal ECG at a detection of 9.3%(48/515). In the 20 patients, the potential patients were mostly featured with incomplete right bundle-branch block(5 cases), complete right bundle-branch block(3 cases), frequent ventricular premature beat (2 cases) and left ventricle high voltage(2 cases), while the chronic patients were mostly featured with incomplete right bundle-branch block(2 cases). Of the 29 chest X-ray films, 17 cases had heart enlargement with a detection rate of 58.6%( 17/29). The selenium content in the hair of 10 adults and 10 children were (0.38±0.09), (0.31±0.12) mg/kg, respectively. Conclusion There are still existing some cases and obvious virulence factors of Keshan disease in Wudu district, Longnan city.

Objective To explore the changes of serum cortisol and its influence on prognosis in children with traumatic brain injury( TBI) . Methods A total of 52 children suffering from TBI hospitalized in SICU of Nanjing Children′s Hospital from June 2014 to December 2015 were analyzed. According to the glasgow coma scale(GCS),they were divided into three groups:mild injury group(19 cases,GCS≥13), moderate injury group(16 cases,8

Objective To observe the effect of Antivirus Compound Capsule in protecting liver and reducing enzyme level in chronic type B hepatitis and acute hepatic injury caused by D-GlaN.Methods Mice model was established by intraperitoneal injecting of D-GlaN 800mg/Kg.The level of ALT and AST was obviously increased after 48hours.Pathological test proved to be acute liver cell damage.Clinically 117 cases of chronic hepatitis B was recruited into a control group(57 cases)and a treatment group(60 cases).The treatment group was treated with Antivims Compound Capsule and Silymarin tablet,and the control group was treated with Silymarin tablet.Besides,both groups were infused with diammonium Compound Capsule Can obviously reduce level of AST(P＜0.001、＜0.01、＜0.01、＜0.001);Three dosage levels of Antivirus Compound Capsule can obviously reduce the degree hepatic pathological changes caused by D-GlaN induced(P＜0.01、＜for the treatment group andtlle control group) between the two groups with P＞0.02.while there was obvious difference of negative conversion rate of AST.[92.98%(53/57)and 78.9%(45/57)for the treaunent group and the consul group]between the two groups with P＜0.01.Conclusion Antivirus Compound Capsule is effective in protecting liver and reducing enzyme level of patients with chronic hepatitis B and acute hepatic injury caused bv D-GlaN.

Objective To investigate the routine ultrasound and contrast-enhanced ultrasound (CEUS) features of hepatic nodules in Budd-Chiari syndrome(BCS) after portacaval anastomosis.Methods Routine ultrasonography and CEUS were performed in 18 BCS patients with hepatic nodules after portacaval anastomosis.Results Appearance after portosystemic anastomosis,multiplicity,small size,presence of peripheral rim and hypervascularization were important ultrasound imaging features of hepatic regenerative nodules in patients with BCS.Nodules showedquick wash-in and slow wash-out pattern in CEUS.Sixteen cases showed center-to-periphery enhancement pattern in arterial phase and hyper-enhancement in portal phase and late phase.Two cases showed periphery-to-center enhancement pattern in arterial phase and periphery enhancement in portal phase and late phase.Conclusions Hepatic regenerative have different features on routine ultrasound and CEUS in patients with BCS after portacaval anastomosis,which are useful for differential diagnosis.

Objective:To optimize the macroporous resin separation process for total flavonoids in papaya. Methods:The content of total flavonoids in papaya was selected as the index, and the resin model, sample solution concentration, ratio of diameter and height, the flow rate of adsorption, type and volume of eluent, type and volume of impurity removing solvent, elution velocity and the other parameters were investigated. Results:The optimal purification process was as follows: the macroporous resin type was D-140, the sample solution concentration was 0. 1 g·ml-1 , the sample volume was 2BV, the ratio of diameter and height was 1∶9, washing the impurities with 3BV water, eluting with 3BV 10% ethanol first followed by 3BV 50% ethanol with 2BV·h-1 , and collecting 50%ethanol elution. The total flavonoids content was 52%. Conclusion:The optimized process can separate and purify the total flavonoids in papaya effectively.

Objective To explore the relationship between dipeptidyl peptidaseⅡ(DPPⅡ) and the pathogenesis of congenital cataract.Methods Eighty SCR rats with congenital cataract were randomly divided into four groups:8,10,12 and 14 weeks groups(n=20).Normal Wistar rats at each age were used as control groups.Immunohistochemical experiments using the streptavidin-biotin immunoper-oxidase method were used to observe the immunoreactivity changes of DPPⅡ in lens in SCR rats at different time(8,10,12,14 weeks).Results The immunoreactivities of DPPⅡ in the lens epithelial cells and fibres of cataractous lenses of all ages were higher than those in control groups.At the 12th and 14th week,immunoreactive staining of DPPⅡwas found to extend into the perinuclear region.Conclusion The immunoreactivity of DPPⅡ is enhanced in cataractous rat lenses.DPPⅡ may be participated in the proteolytic modification of lens proteins during cataractogenesis.

Streptomyces sp.X-435 isolated from a soil sample collected in the suburbs of Beijing was proved to be a produce Virginiamycin.To improve the productivity of the Virginiamycin of Streptomyces sp.X-435,the spores of strain X-435 were treated with UV.The three types of colony,strawhat,wrinkled,blad,were isolated on Gaose's medium plates after mutation.Among them,the colonies of strawhat type exhibited positive mutation and were picked up as objects of screening.After five generation of mutation,the mutant F5-25-u-28 was selected which potency of Virginiamycin was about 20times higher than that of the beginning strain by flask fermentation and was also genetic stable.

Objective To explore the clinical effect of early cognitive rehabilitation on cognitive,physical function and quality of life in the patients transferred from intensive care unit (ICU).Methods A total of 120 cases of patients who were transferred from ICU to general wards was randomly divided into the control group and the observation group with 60 cases in each group.The control group was given symptomatic treatment according to their original disease,without cognitive intervention.On the basis of the control group,the observation group was treated with cognitive rehabilitation training,2 times / week,2 h/ times,12 weeks of treatment.The cognitive function,physical function and quality of life of two groups were compared with the memory and executive screening scale (MES),the daily activity scale (ADL),and the concise Health Survey (SF-36) before and after treatment.Results Compared to before treatment,after treatment,MES,SF-36 (in addition to bodily pain) and ADL scale project score in the observation group was significantly higher,SF-36 bodily pain scores decreased significantly,all the differences were statistically significant (P ＜ 0.05);Compared to the control group,after treatment,the MES,SF-36 (in addition to bodily pain) and ADL scale scores in the observation group of were significantly increased,the bodily pain score of SF-36 in the observation group compared with the control group decreased significantly,all the differences were statistically significant (P ＜ 0.05).Conclusions Early cognitive rehabilitation can significantly improve the cognitive,physical function and quality of life in the patients transferred from ICU,and it is worthy of clinical reference.