1.The application of standardization to oral medicine.
Chinese Journal of Stomatology 2005;40(6):518-520
2.Relationship between Renal Nitric Oxide and Glomerular Hyper filtration in Experimental Diabetic Rats
ming-hua, LIU ; shu-zhen, SUN ; qian, LI ; yi, WANG
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To explore the mobile changes of nitric oxide(NO) and the relationship between nitric oxide and glomerular hyperfiltration in experimental diabetic rats.Methods Experimental diabetes mellitus(DM) was induced in rats with streptozotocin(STZ).The levels of NO and NOS in renal tissue homogenate were assayed after establishment of diabetesat the 4 th,8 th,12 th week.At the same time, renal morphology in diabetic rats was examined by light microscope and image of computer.Results The contents of NO and NOS in renal homegenates were evidently increased at 4 th week,and decreased gradually from 8 th week(P
6.Influence of fiber posts on the fracture resistance of endodontically treated premolars with different dental defects.
Qian-Qian HOU ; Yi-Ming GAO ; Lei SUN
International Journal of Oral Science 2013;5(3):167-171
This study aimed to evaluate the influence of quartz fiber post placement on the fracture resistance of endodontically treated premolars with different dental defects under dynamic loading. Fifty extracted single-rooted mandibular premolars were randomized into five groups. Each group was prepared according to numbers of residual walls ranged from 0 to 4. Then each group was divided into two subgroups with one restored with quartz fiber posts and the other without posts. In no-post groups, gutta percha point 2 mm below cemento-enamel junction was removed. Composite resin was adapted to the well and used to shape the core directly. Each tooth was restored with a complete metal crown. Dynamic loading was carried out in a masticatory simulator with a nominal load of 50 N at 2 Hz for 300 000 loading cycles. Then a quasi-statically load was applied in a universal testing machine 30° to the long axis with a crosshead speed of 1 mm⋅min(-1) until fracture. Data were analyzed with one-way analysis of variance and pairwise comparison (P<0.05). No specimens failed during dynamic loading. The fracture resistance enhanced with the increase of numbers of coronal walls and the differences were significant (P<0.05). Placement of fiber posts had a significant effect when fewer than two walls remained (P<0.05), but it had no significant influence in groups with two, three or four walls (P>0.05). Fiber post did not change failure mode, and the fracture pattern was mainly favorable. More dentin walls need to be retained in clinic. When no less than two walls remained, a fiber post is not always necessary.
Analysis of Variance
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Bicuspid
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Composite Resins
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Dental Restoration Failure
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Dental Stress Analysis
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Dentin
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physiology
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Humans
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Post and Core Technique
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Quartz
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Tooth Fractures
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prevention & control
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Tooth, Nonvital
7.Health status of mothers undergoing in vitro fertilization and their offspring.
Qian-Qian WANG ; Yi-Min ZHU ; Ming-Yuan WU
Journal of Zhejiang University. Medical sciences 2009;38(5):515-520
OBJECTIVETo review the health status of mothers undergoing in vitro fertilization-embryo transfer (IVF) and their offspring.
METHODSHealth status of 210 IVF mothers and offspring performed from January 1, 2001 through December 31, 2002 was compared with that of those who conceived naturally retrospectively.
RESULTA significant differences were found in rates of multiple births, hypertensive disorders in pregnancy,cesarean section, preterm delivery,low (and very low) birth weight infant,malformation(33.33 % with compared 1.72 %, 10.87 % with compared 4.59 %, 89.85 % with compared 65.57 %, 24.64 % with compared 6.27 %, 25.68 % with compared 8.23 %, 3.83 % with compared 1.17 %, all P<0.01) between IVF and those conceived naturally. The rates of hypertensive disorders in pregnancy,preterm delivery, low (and very low) birth weight infant were related to the rate of multiple births OR. 95 %CI were 3.49, 1.16-10.50; 13.65, 5.39-34.58; 14.91, 5.53-40.17; respectively. There was no statistical difference in neonatal outcome between normal IVF and intra-cytop-lasmic sperm injection (P>0.05); no difference in height and weight between full-term and preterm (P>0.05).
CONCLUSIONAssisted reproductive technologies increase multiple births rate, resulting in high rates of hypertensive disorders, preterm delivery, low (and very low) birth weight infant.
Adult ; Cesarean Section ; statistics & numerical data ; China ; epidemiology ; Embryo Transfer ; Female ; Fertilization in Vitro ; Health Status ; Humans ; Hypertension, Pregnancy-Induced ; epidemiology ; Multiple Birth Offspring ; Pregnancy ; Premature Birth ; epidemiology ; Retrospective Studies
8.Studies on baicalin ethylcellulose microspheres for intranasal administration.
Yu-yi QIAN ; Liu-hong ZHANG ; Li-wei GUO ; Hua-xu ZHU ; Ting-ming FU
China Journal of Chinese Materia Medica 2014;39(24):4787-4791
In this study, solvent evaporation method was used to preparing baicalin ethylcellulose microspheres for intranasal administration. The prepared microspheres were round with certain rough surface. The average drug loading and entrapment efficiency was (33. 31 ± 0. 045)% , (63. 34 ± 0. 11)% , respectively. As the characteristic crystalline peaks of baicalin were observed in the microspheres sample, the result of X-ray diffractometric analysis indicated that the baicalin was present in crystalline form after its entrapment in ethylcellulose matrix. By investigating the thermogram of microspheres sample, it was found that endothermic peak of baicalin was shifted from 211. 8 °C to 244. 2 °C and associated with the first broad endothermic peak of ethylcellulose. This could confirm that baicalin was loaded into ethylcellulose, nor simply physical mixture. The powder flowability test exhibited that the specific energy of microspheres was 3. 57 mJ . g-1 and the pressure drop was 2. 22 mBar when air kept the speed of 2 mm . s-1 through the powder bed with the force was 15 kPa. The consequence of the baicalin in vitro released from microspheres showed that the pure baicalin sample displayed faster (90%) release than microspheres sample (75%) in 7 h. Fitting model for release curve before 7 h, the results showed that the pure baicalin sample and the microsphere sample accorded with first order model (R2 = 0. 990 4) and Riger-Peppas model(R2 = 0. 961 2), respectively. Ex vivo rabbit nasal mucosa permeability experiment revealed that the value of cumulative release rate per unit area of the microsphere sample was 1. 56 times that of the pure baicalin sample. This provided the foundation for the in vivo pharmacokinetic study.
Administration, Intranasal
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Air Pressure
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Animals
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Cellulose
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analogs & derivatives
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chemistry
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Drug Compounding
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methods
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Flavonoids
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administration & dosage
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chemistry
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pharmacokinetics
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Male
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Microspheres
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Mucous Membrane
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metabolism
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Particle Size
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Powders
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Rabbits
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Solvents
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X-Ray Diffraction
9.Influence of Brain Hypoxia-Ischemia on Expression of Glucose Transporter 1 Genes and Glucose Transpsorter 3 Genes in Neonatal Rats
zheng, CHEN ; hui-jin, CHEN ; ming-hua, JIANG ; long-hua, QIAN ; guan-yi, CHEN
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To understand the mechanism of cerebral energy failure after hypoxia ischemia at the molecular level and to establish the protocol for the safe and effective treatment of hypoxic-ischemic encephalopathy(HIE).Methods One hundred neonatal rats were divided into normal control group and hypoxic-ischemic(HI) group. SD rats of both groups were decapitated at the time of 2 h,24 h,48 h,72 h and 7 d after HI.These tissues of cerebrum,cortex and hippocampus were taken out to explore the influence of HI on the expression of GLUT1 and GLUT3 genes with the method of RT-PCR.Results There was an enhancement in the expression of GLUT1 and GLUT3 genes with the increasing of day age. The expression was more intense in hippocampus than that in cortex. However, HI could significantly enhance the expression of GLUT genes. The expression was higher in cortex than that in hippocampus. The expression of two genes reached the peak at 24 h after HI, but was significantly lower than that in control group at 7 d after HI.Conclusion The increased expression of GLUT genes can maintain the energy supplement for the brain and delay a cascade reaction of cerebral energy failure.
10.Effects of electro-acupuncture on functional performance and the expression of apoptosis-related proteins Bcl-2 and Bax in the hippocampal CA1 region of rats with cerebral infarction
Qian YU ; Xiaohong LI ; Ling HUANG ; Ming CHENG ; Yi LI ; Xinpu PENG ; Ke YU
Chinese Journal of Physical Medicine and Rehabilitation 2012;34(4):245-249
Objective To observe effects of electro-acupuncture (EA) on the expression of apoptosis related protein B-cell lymphoma/leukemia gene 2 (Bcl-2) and Bcl-associated protein X (Bax) in the hippoeampal CA1 region of rats with cerebral infarction,and on their behavior. MethodsAn animal model of cerebral ischemia was established by right side middle cerebral artery occlusion using thread in 48 male,adult Wistar rats,who were then randomly divided equally into an EA group and a control group.Both groups were sub-divided into 1 week,2 weeks and 3 weeks subgroups.The EA group began receiving EA 24h after the occlusion,applied at the Baihui (DU20) and Dazhui (BU14) points,once daily,for one,two or three weeks.The control group was reared conventionally and was not given any special treatment.The rats'learning and memory,motion and neural function were evaluated.The expression of Bcl-2 and Bax in the CA1 region of the hippocampus on the infarcted side and changes of apoptosis indexes were detected using immunohistochemical techniques.ResultsThe learning,memory,motion and neural function of the EA group rats were better on average than those of the control group at all observation time points.The expression of Bcl-2 protein increased along with reduced expression of Bax protein in the EA group significantly more than among the controls.TUNEL positive cells in the CA1 region of the hippocampus were reduced significantly in the EA group compared with the control group.ConclusionsBehavioral ability after cerebral infarction can be improved by EA at the Baihui (DU20) and Dazhui (BU14) points.EA can up-regulate the expression of Bcl-2 and down-regulate the expression of Bax and reduce TUNEL positive cells in the CA1 region of the hippocampus after cerebral infarction,which might be the mechanism of its neuroprotection.