Humans ; Mouth Diseases ; classification ; diagnosis ; therapy ; Mouth Mucosa ; pathology ; Oral Medicine ; standards ; Reference Standards

Chromatography, Gas ; methods ; Ethanol ; blood ; Humans ; Methanol ; blood

Objective To explore the mobile changes of nitric oxide(NO) and the relationship between nitric oxide and glomerular hyperfiltration in experimental diabetic rats.Methods Experimental diabetes mellitus(DM) was induced in rats with streptozotocin(STZ).The levels of NO and NOS in renal tissue homogenate were assayed after establishment of diabetesat the 4 th,8 th,12 th week.At the same time, renal morphology in diabetic rats was examined by light microscope and image of computer.Results The contents of NO and NOS in renal homegenates were evidently increased at 4 th week,and decreased gradually from 8 th week(P

Benzodiazepines ; blood ; Case-Control Studies ; Chromatography, Gas ; Humans

Diabetes Insipidus, Neurogenic ; diagnosis ; metabolism ; Humans ; Pituitary Neoplasms ; diagnosis ; secondary

This study aimed to evaluate the influence of quartz fiber post placement on the fracture resistance of endodontically treated premolars with different dental defects under dynamic loading. Fifty extracted single-rooted mandibular premolars were randomized into five groups. Each group was prepared according to numbers of residual walls ranged from 0 to 4. Then each group was divided into two subgroups with one restored with quartz fiber posts and the other without posts. In no-post groups, gutta percha point 2 mm below cemento-enamel junction was removed. Composite resin was adapted to the well and used to shape the core directly. Each tooth was restored with a complete metal crown. Dynamic loading was carried out in a masticatory simulator with a nominal load of 50 N at 2 Hz for 300 000 loading cycles. Then a quasi-statically load was applied in a universal testing machine 30° to the long axis with a crosshead speed of 1 mm⋅min(-1) until fracture. Data were analyzed with one-way analysis of variance and pairwise comparison (P<0.05). No specimens failed during dynamic loading. The fracture resistance enhanced with the increase of numbers of coronal walls and the differences were significant (P<0.05). Placement of fiber posts had a significant effect when fewer than two walls remained (P<0.05), but it had no significant influence in groups with two, three or four walls (P>0.05). Fiber post did not change failure mode, and the fracture pattern was mainly favorable. More dentin walls need to be retained in clinic. When no less than two walls remained, a fiber post is not always necessary.
Analysis of Variance ; Bicuspid ; Composite Resins ; Dental Restoration Failure ; Dental Stress Analysis ; Dentin ; physiology ; Humans ; Post and Core Technique ; Quartz ; Tooth Fractures ; prevention & control ; Tooth, Nonvital

OBJECTIVETo review the health status of mothers undergoing in vitro fertilization-embryo transfer (IVF) and their offspring.

METHODSHealth status of 210 IVF mothers and offspring performed from January 1, 2001 through December 31, 2002 was compared with that of those who conceived naturally retrospectively.

RESULTA significant differences were found in rates of multiple births, hypertensive disorders in pregnancy,cesarean section, preterm delivery,low (and very low) birth weight infant,malformation(33.33 % with compared 1.72 %, 10.87 % with compared 4.59 %, 89.85 % with compared 65.57 %, 24.64 % with compared 6.27 %, 25.68 % with compared 8.23 %, 3.83 % with compared 1.17 %, all P<0.01) between IVF and those conceived naturally. The rates of hypertensive disorders in pregnancy,preterm delivery, low (and very low) birth weight infant were related to the rate of multiple births OR. 95 %CI were 3.49, 1.16-10.50; 13.65, 5.39-34.58; 14.91, 5.53-40.17; respectively. There was no statistical difference in neonatal outcome between normal IVF and intra-cytop-lasmic sperm injection (P>0.05); no difference in height and weight between full-term and preterm (P>0.05).

CONCLUSIONAssisted reproductive technologies increase multiple births rate, resulting in high rates of hypertensive disorders, preterm delivery, low (and very low) birth weight infant.

Adult ; Cesarean Section ; statistics & numerical data ; China ; epidemiology ; Embryo Transfer ; Female ; Fertilization in Vitro ; Health Status ; Humans ; Hypertension, Pregnancy-Induced ; epidemiology ; Multiple Birth Offspring ; Pregnancy ; Premature Birth ; epidemiology ; Retrospective Studies

We analyzed the laboratory detection results from the first case of human infected with avian influenza (H5N6) in Hubei Province,in order to provide a better basis for preventing and controlling human avian influenza in the future and a detected strategy for the detection of suspected cases of human avian influenza for the staff in the laboratory.The case data of epidemiological survey and related laboratory detection results of specimens of infection virus from the different time piont and kinds of specimens were analyzed by descriptive epidemiological methods.The H5N6 nucleic acid from the only early sputum specimens were detected,and while the others were not detected.In conclusion the different specimens of the doubtful H5N6 case should be collected,and the early sputum samples are very important and should be collected and detected.

Objective To observe effects of electro-acupuncture (EA) on the expression of apoptosis related protein B-cell lymphoma/leukemia gene 2 (Bcl-2) and Bcl-associated protein X (Bax) in the hippoeampal CA1 region of rats with cerebral infarction,and on their behavior. MethodsAn animal model of cerebral ischemia was established by right side middle cerebral artery occlusion using thread in 48 male,adult Wistar rats,who were then randomly divided equally into an EA group and a control group.Both groups were sub-divided into 1 week,2 weeks and 3 weeks subgroups.The EA group began receiving EA 24h after the occlusion,applied at the Baihui (DU20) and Dazhui (BU14) points,once daily,for one,two or three weeks.The control group was reared conventionally and was not given any special treatment.The rats'learning and memory,motion and neural function were evaluated.The expression of Bcl-2 and Bax in the CA1 region of the hippocampus on the infarcted side and changes of apoptosis indexes were detected using immunohistochemical techniques.ResultsThe learning,memory,motion and neural function of the EA group rats were better on average than those of the control group at all observation time points.The expression of Bcl-2 protein increased along with reduced expression of Bax protein in the EA group significantly more than among the controls.TUNEL positive cells in the CA1 region of the hippocampus were reduced significantly in the EA group compared with the control group.ConclusionsBehavioral ability after cerebral infarction can be improved by EA at the Baihui (DU20) and Dazhui (BU14) points.EA can up-regulate the expression of Bcl-2 and down-regulate the expression of Bax and reduce TUNEL positive cells in the CA1 region of the hippocampus after cerebral infarction,which might be the mechanism of its neuroprotection.

Objective To determine,the clinical significance of serum myocardial enzymes (Mb,cTNI, CK,CK-Mb,AST,LDH) in the classification of the disease severity of non-cardiogenic critically-ill patients. Compared with APACHEⅡscore concerned as the standard diagnosis of the critical ills,these biomarkers were investigated for the evaluation possibility of the degree and the prognosis of the critical ills.Method Patients admitted to our EICU were consecutively collected for the research from April to December in 2005 and the myocardial enzymes,and routine serum biochemical test and APACHEⅡscore were detected simultaneously.All the patients were classified to three groups according to the APACHEⅡscore (mild group,APACHEⅡ25) and two groups (survive group and death group) according to the prognosis.All the patients were followed up till recovery/discharge or death. Covariance,Wilcoxon and x~2 were used for the statistical analysis.Results The myocardial enzymes rose when the disease deteriorated and the APACHEⅡscore went up.AST,LDH,CK,CK-Mb,Mb were significantly different in the three groups according to the APACHEⅡscore (P