Adult ; Altitude ; Female ; Heart ; physiology ; Humans ; Male ; Occupational Health ; Railroads ; Tibet

Adult ; China ; Female ; Humans ; Male ; Middle Aged ; Program Evaluation ; Smoking Cessation ; psychology ; statistics & numerical data ; Young Adult

0.05);Compared to Gram-negative bacilli group of sputum culture,Gram-positive cocci group had significant diffe-rence in the incidence of gastrointestinal dysfunction and microcirculatory disorders(Pa

In order to obtain the phase I clinical trial data safely and accurately , the subjects management is very important .The purpose of this paper is to introduce the subjects management model during recruitment , screening and observation and discuss the phase I subjects management strategy , according to the author ’ s practical experience and the working model of phase I clinical trials in hospital .

Administration, Oral ; Adult ; Atrial Flutter ; drug therapy ; physiopathology ; Digoxin ; therapeutic use ; Echocardiography ; Female ; Fetal Diseases ; drug therapy ; physiopathology ; Humans ; Hydrops Fetalis ; drug therapy ; physiopathology ; Infant, Newborn ; Male ; Pregnancy

OBJECTIVETo explore the clinical effect of the method of rotation descent step by step for unilateral complete cleft lip with.

METHODSThe method of rotation descent step by step was used to repair unilateral complete cleft lip. Point X was located on the angular bisector of 123, rotation descent step by step and orbicularis oris degloved dissection were used. Nasal deformities were corrected at the same stage.

RESULTSFrom Oct. 2006 to Oct. 2008, 68 cases were treated with primary healing. 42 cases were followed up for less than one year. Among them, 3 cases showed asymmetric lip height, and 6 cases showed asymmetric lip width. 26 cases were followed up more than one year with only two cases of asymmetric lip width.

CONCLUSIONSThe method of rotation descent step by step is very suitable for repairing unilateral complete cleft lip. The restoration of the malpositioned tissue is especially emphasized in this method.

Child, Preschool ; Cleft Lip ; surgery ; Female ; Follow-Up Studies ; Humans ; Infant ; Male ; Reconstructive Surgical Procedures ; methods

In order to amplify F gene of NDV HeB02 strain, one pair of primers was designed according to the GenBank sequence, and a 1.66 kb F gene fragment was obtained by RT-PCR. Sequence analysis indicated that the homologies of the nucleotide sequence of HeB02 strain to those of F48 E9, La Sota and Clone30 strains were 88.1%, 84.9% and 83.8% respectively. The expression plasmid pSV-F was constructed by inserting the F gene into the pVAX1 vector, and transfected into the cultured COS 7 cell line via liposomes. The specific 5.9 kD protein was detected by SDS-PAGE and the immunogenicity of the expressed F protein was confirmed by Western blot, ELISA and neutralization test. 3 week-old SPF chickens were subcutaneously immunized twice at week 0 and 3 with 50 microg DNA of plasmid pSV-F by electroporration. 5 weeks later, all chickenss were challenged with 100 x EID50 of NDV HeB02 strain, 1 week post challenge all chickenss were sampled by larynx swabbing to isolate virus and the HI level of NDV was measured. The results indicated that the virus isolation was negtive in all vaccinated chickenss and positive in all control chickens. The HI titres reached to 8.3log2 +/- 1.30 and 7.2log2 +/- 1.23 induced by NDV vaccine and positive cells (pSV-F), respectivily, the HI titres induced by Control cells (pVAX1) was not detected. Furthermore, the HI titres reached to 9.8log2 +/- 1.55 and 8.9log2 +/- 1.77 in vaccinated group with NDV vaccine and positive cells (pSV-F), respectivily, were sinificantly higher than that of the control cells (pVAX1) immunized group( HI titers was 3.0 log2 +/- 1.40, P < 0.01) after challenge. These results showed that the plasmid pSV-F could be as a candidate of DNA vaccine to provide protective immune response against NDV infection.
Animals ; COS Cells ; Cercopithecus aethiops ; Chickens ; Cloning, Molecular ; Hemagglutination Inhibition Tests ; veterinary ; Newcastle Disease ; immunology ; prevention & control ; Newcastle disease virus ; classification ; genetics ; immunology ; Reverse Transcriptase Polymerase Chain Reaction ; Transfection ; Vaccination ; Vaccines, DNA ; genetics ; immunology ; Viral Fusion Proteins ; genetics ; immunology ; Viral Vaccines ; genetics ; immunology

AIM: To investigate therapeutic efficiency of amniotic extraction on dry eye in rabbit model induced by topical benzalkonium chloride (BAC).
METHODS: Totally 26 rabbits (26 right eyes) with dry eye model were studied and divided into two groups:group A (control group with PBS eye drops, n = 13) and group B ( amniotic extraction group, n = 13). Another two rabbits were chosen as normal control. The SchirmerⅠ tests ( S Ⅰ t) and corneal fluorescein staining ( FL) were made, and the tear total protein content, amylase activity, lactoferrin, lysozyme contents, goblet cell density were performed in two groups before treatment and 1, 2, 4 and 8 wk after treatment.
RESULTS: There were significant differences in SIT, FL scores, lysozyme activity and goblet cell density among different groups at different time points (P<0. 05). But, there was no significant differences in SⅠt, FL scores, lysozyme activity and goblet cell density between two groups before treatment (P>0. 05). After 8wks' treatment with PBS, the mean differences of the group A showed great changes in SⅠt, lysozyme and goblet cell density compared with those before treatment ( P < 0. 05); but there was no significant differences in FL scores compared with those before treatment (P>0. 05). As for group B, 8wks after treatment, there were statistical changes in SⅠt, FL, lysozyme (P<0. 05); but there was no significant differences in goblet cell density compared with those before treatment ( P > 0. 05). It was evident that statistical differences were observed in S Ⅰ t, FL scores, lysozyme activity and goblet cell density between two groups at each time point (P<0. 05). However, there were no significant differences in total protein, lactoferrin, amylase activity at different time points (P>0. 05). Meanwhile there was no significant differences in total protein, lactoferrin, amylase activity between two groups before treatment ( P > 0. 05 ). But there were significant differences in total protein, lactoferrin, amylase activity between two groups after 4 and 8 wks'treatment (P<0. 05).
CONCLUSION: Amniotic extraction has significant therapeutic effect on the dry eye in rabbit model.

Ethanol is metabolized by alcohol dehydrogenase (ADH) and cytochrome P450 2E1 (CYP2E1) metabolizing enzymes,and drugs that affect or metabolize enzymes metabolised by ADH and CYP2E1 are likely to interact with ethanol.The effect of ethanol on the drugs may produce pharmacokinetic or pharmacodynamic changes.We will review the interaction mechanism of various drugs with ethanol.

Objective To analyze the the characteristics,risk factors and treatment of trastuzumab-induced hepatotoxicity in patients with breast cancer.Methods To analyze by a case report of trastuzumab-induced hepatotoxicity in a patient with breast cancer in practice combined with 4 related case reports in literatures.Results and conclution Trastuzumab-induced liver toxicity may have the following characteristics:early occurrence;previous use of medication with the side effect of liver damage may be one of the causes of hepatotoxicity;the grade of hepatotoxicity often reaches grade 3 and grade 4;long duration of liver function recovery;hepatotoxicity induced by trastuzumab may be dose-related.Focusing on past medical history and medication history and regular monitoring of liver function may be one of the effective measures to determine hepatotoxicity intensively and to avoid serious complications.