1.Pediatric pilocytic astrocytoma with monomorphous pilomyxoid features and plexiform pattern: report of a case.
Chinese Journal of Pathology 2011;40(8):565-566
Astrocytoma
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metabolism
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pathology
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surgery
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Brain Neoplasms
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metabolism
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pathology
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surgery
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Child, Preschool
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Diagnosis, Differential
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Ependymoma
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metabolism
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pathology
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Female
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Follow-Up Studies
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Glial Fibrillary Acidic Protein
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metabolism
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Humans
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Neoplasm Recurrence, Local
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Neoplasm Staging
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Neurofibroma, Plexiform
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metabolism
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pathology
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S100 Proteins
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metabolism
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Vimentin
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metabolism
2.Clinical study of reformed laparoscopic-assisted vaginal hysterectomy of large uterus with sutureless of cardinal and uterosacral ligament
Xinling ZHOU ; Xinyong JING ; Yi LI
Chinese Journal of Postgraduates of Medicine 2011;34(36):11-13
Objective To compare the clinical efficacy between reformed laparoscopic-assisted vaginal hysterectomy with sutureless of cardinal and uterosacral ligament(LAVH)and trans abdominal hysterectomy(TAH)for large uterus.Methods A retrospective study was performed using 106 reformed LAVH patients(observation group)and 100 TAH patients(control group)whose uterus were big(≥ 12weeks of gestation),the information was observed in perioperative period.Results Anal exsufflation time,hospitalization time after operation and postoperative pain time in observation group[(25.4 ± 3.3)h,(6.5 ±1.5)d,(16.9 ± 2.4)h]were significantly shorter than those in control group[(42.4 ± 5.3)h,(8.5 ± 1.5)d,(30.6 ± 5.3)h](P < 0.05),the rate of complication in observation group[5.7%(6/106)]waa lower than that in control group[10.0%(10/100)](P< 0.05).Conclusion As to large uterus,reformed LAVH has several advantages over TAH,the technique is easy to learn,operation time is short,and the rate of complication is low.
3.The Comparative Study of Intonation Pronunciation in Preschool Children with Hearing Impairments and Normal Children
Ling YI ; Lei ZHANG ; Jing ZHOU
Journal of Audiology and Speech Pathology 2016;24(3):224-227
Objective This study aims to study the characteristics of intonation pronunciation in preschool children with hearing impairments .Methods A total of 30 of 4~5 years old children with hearing impairment and 30 of 4~5 years old children with normal hearing were enrolled in this study .With statement intonation and ques-tion intonation as test material ,the compartment between the children in two groups were obtained .Results Pre-school children were generally increased the ending point and slope to distinguish statement intonation .There were still differences between hearing -impaired children and normal children .Under statement intonation condition :the children with hearing impairments showed a significantly lower level of the slope of the high and level boundary tone than the normal children ,the starting point and the ending point of the falling boundary tone in hearing -impaired children were significantly lower than those of in normal children ;Meanwhile ,under question intonation condition :com-pared with normal children ,the slope of the high and level boundary tone ,the rising boundary tone and the falling -rising boundary tone in hearing -impaired children were significantly lower .The starting point ,the ending point ,the high and level boundary tone ,and the falling boundary tone in hearing -impaired children were significantly lower .Conclusion To implement targeted therapy during question intonation training for hearing -impaired children ,the acoustics characteristics of boundary tone are required to assess and monitor the effectiveness of rehabilitation .
4.Mechanism about therapeutic effect of meisoindigo on psoriasis via down-regulation of the TLR4-TAK-NF-kappaB pathways.
Haijing ZHANG ; Yi ZHANG ; Jing JIN ; Wanqi ZHOU ; Xiaoguang CHEN
Acta Pharmaceutica Sinica 2013;48(4):503-7
Meisoindigo is an indigo natural derivative commonly used in anti-cancer therapy. In the clinical application, it was also found to have good therapeutic effect on psoriasis. In order to further understand its mechanism of action, human normal keratinocyte cell line HaCaT and RAW 264.7 were used to identify if meisoindigo could affect the inflammatory factors such as NO and other important cytokines which were highly involved in psoriasis. Our results indicated that meisoindigo decreased the production of NO in LPS-stimulated RAW 264.7 cells and reduced the expression of cytokines in LPS-stimulated HaCaT cells. And TLR4-TAK-NF-kappaB was a possible pathway mainly involved in the attenuation of iNOS and pro-inflammatory cytokine production by meisoindigo, which may take part in the therapeutic effect of meisoindigo on psoriasis.
5.Effect of 3-n-butylphthalide pretreatment on expression of the HSP70 after brain ischemia/reperfusion.
Yi ZHOU ; Li-jing NIU ; Feng-miao QI ; Li GUO
Chinese Journal of Applied Physiology 2015;31(2):136-140
OBJECTIVETo explore the effect of 3-n-butylphthalide pretreatment on the delayed neuronal death(DND) and the expreesion of heat shock protein70 (HSP70) in rat hippocampus after ischemia/ reperfusion.
METHODSAll rats were randomly divided into sham group (n = 36), total cerebral ischemia (TCI) group (n = 36), butylphthalide (NBP) group (n = 6), NBP + TCI group( n = 36), quercetin + NBP + TCI group (n = 6), dimethyl sulfoxide (DMSO) + NBP + TCI group (n = 6). The model of total cerebral ischemia/reperfusion was established by blocking vertebral arteries and carotid arteries. In sham group, TCI group and NBP group, the animals were further divided into instantly, 6 h, 12 h, 1 d, 3 d, 5 d groups according to the time interval after sham operation or TCI. Histological changes of the hippocampus were evaluated using thionin staining under light microscope by determining the delayed neuronal death (DND) and the expression of HSP70 was assayed using immunohistochemistry.
RESULTSNBP pretreatment could reduce delayed neuronal death in CA1 of hippocampus induced by TCI-reperfusion injury in rats, and up-regulated the expression of HSP70 in CA1 hippocampus of brain ischemic/reperfusion for 5 days. Quercetin blocked the acquirement of the brain ischemic tolerance induced by NBP preconditioning.
CONCLUSION3-n-butylphthalide (NBP) prevents the neurons from ischemia/reperfusion injury through upregulating the expression of HSP70.
Animals ; Benzofurans ; pharmacology ; CA1 Region, Hippocampal ; cytology ; pathology ; Cell Death ; Cerebral Infarction ; drug therapy ; HSP70 Heat-Shock Proteins ; metabolism ; Ischemic Preconditioning ; Neurons ; cytology ; Rats ; Rats, Wistar ; Reperfusion Injury ; drug therapy
6.Effects of dexmedetomidine on PI3K/Akt pathway in hippocampus of propofol anesthetized neonatal rats
Lifang ZHOU ; Yi WEI ; Jing LYU ; Yubo XIE
The Journal of Clinical Anesthesiology 2017;33(1):59-62
Objective To explore the effect of dexmedetomidine on phosphoinositide 3-kinase/protein kinase B (PI3K/Akt ) pathway in hippocampus of propofol anesthetized neonatal rats. Methods Eighty Sprague-Dawley male rats,aged 7 days,weighing 10-1 5 g,were randomly divided into 8 groups (n= 10 each):normal saline group (group N),DMSO group (group D),intralipid group (group I),propofol group (group P),dexmedetomidine 25 μg/kg,50 μg/kg and 75 μg/kg +propofol 100 mg/kg groups (groups PD25 ,PD50 and PD7 5 ),LY294002 25 μg + dexmedetomidine 75μg/kg + propofol 100 mg/kg group (group LYPD).The hippocampus of rats in all groups were taken 2 h after the animals fully awake.The ultrastructure of hippocampal neurons was observed by transmission electron microscope.The pAkt-(ser473 )protein and Akt protein in the hippocampus were evaluated by Western blot analysis.Results There was no significant difference in the expression of Akt protein among the eight groups.Compared with group N,the expression of pAkt (ser473)protein was significantly down-regulated in groups P,PD25 ,PD50 ,PD7 5 and LYPD (P <0.05).Compared with group P,the expression of pAkt (ser473)protein was increased significantly in groups PD7 5 and LYPD (P <0.05).Compared with group PD7 5 ,the expression of pAkt (ser473) protein was significantly down-regulated in group LYPD (P <0.05 ).The structure of hippocampal neurons was normal in groups N,I and D.Nuclear nuclei swelling,chromatin decreasing and mito-chondrion vacuolar degeneration were observed in group P while improved gradually with dexmedeto-midine in a dose-dependent manner in groups PD25 ,PD50 and PD7 5 .Neurons karyopyknosis,partial dissolution of nuclear membrane,chromatin condensation,mitochondria vacuolar degeneration were observed in group LYPD.Conclusion Dexmedetomidine pretreatment provides neuroprotection against propofol-induced hippocampal destruction by preserving PI3K/Akt pathway activity in the de-veloping brains.
7.Balloon dilatation of the cervix and pubeisheng on labor induction of full-term pregnancy use of oxytocin, mode of delivery and the analysis of the occurrence of adverse reactions
Lifang CHEN ; Jing ZHOU ; Yanfei ZHU ; Xia LUO ; Yi WANG
Chinese Journal of Biochemical Pharmaceutics 2017;37(4):429-431
Objective To investigate the effect of labor induction of full-term pregnancy use of oxytocin,and the incidence of adverse reactions of mode of delivery of cervical dilatation balloon with pubeisheng.Methods The clinical data of full-term pregnant women who were delivered in our hospital from October 2014 to November 2015 were retrospectively analyzed,According to the induction of labor is divided into the way of pubeisheng group and cervical dilation balloon group.The differences of delivery mode,oxytocin utilization,maternal and neonatal outcomes and adverse reactions were observed between the two groups.Results Cervical dilatation balloon group vaginal delivery rate was 90.38%,higher than that of pubeisheng group(P<0.05); oxytocin use rate of 11.54%,lower than that of pubeisheng group(P<0.05); Cervical dilatation balloon group first,second and third labor time is shorter pubeisheng group(P<0.001); The cervical dilation balloon group bleeding more than pubeisheng group(P<0.001),two groups of infection,urinary retention complication rate had no significant difference.Two groups of neonatal aspiration pneumonia incidence rate had no significant difference after the birth of 1min Apgar score difference,and cervical dilatation balloon group after the birth of 5min Apgar was higher than that of pubeisheng group(P<0.05).Conclusion The application of cervical dilation balloon in the induction of labor in term pregnancy is better,which can significantly improve the rate of vaginal delivery,reduce the use rate of oxytocin and shorten the labor process,and has the value of clinical application.
8.Practical consideration on vascular patterns in pathologic diagnosis of central nervous system tumors.
Jing ZHOU ; Guo-yi YANG ; Nan-yun LI
Chinese Journal of Pathology 2011;40(9):641-644
Astrocytoma
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blood supply
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pathology
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Capillaries
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pathology
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Central Nervous System Neoplasms
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blood supply
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pathology
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Diagnosis, Differential
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Ependymoma
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blood supply
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pathology
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Glioblastoma
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blood supply
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pathology
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Hemangioblastoma
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blood supply
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pathology
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Humans
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Hyperplasia
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Microvessels
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pathology
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Neoplasms, Neuroepithelial
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blood supply
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pathology
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Oligodendroglioma
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blood supply
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pathology
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Paraganglioma
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blood supply
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pathology
9.Analysis of the statistics of graduates students in PUMC hospital
Xin ZHOU ; Jing WANG ; Zhi-yi ZHANG ; Chao NI
Chinese Journal of Medical Education Research 2011;10(9):1074-1076
The author analyzes the basic information in recent years,including overall students scale,students' age,application trends,etc.Some suggestions are made,regarding to recruiting plan,applicant qualification,recruiting mode,etc,in order to further improve the recruiting and graduate management.
10.Expression of CD147 protein in human cervical carcinoma
Xi ZHOU ; Jing JI ; Yi WU ; Pengsheng ZHENG
Journal of Xi'an Jiaotong University(Medical Sciences) 2010;31(1):14-16,25
Objective To investigate the expression of CD147 and its significance in human cervical carcinoma. Methods Western blotting and immunohistochemical staining were used to detect CD147 expression in cervical cancer or normal cervix uteri tissues. Results CD147 protein was expressed in all of cervical carcinomas (41/41, 100.0%) and most of normal cervix uteri tissues (11/12, 91.7%). CD147 with different molecular weights were present in cervical tissues. The percentage of CD147-positive cells and the expressional level of CD147 were higher in cervical carcinomas than in normal cervix (P<0.05). Conclusion CD147 might be recognized as a marker of cell proliferation. High expression of CD147 in cervical carcinomas suggests that it might be a potential target for cervical carcinoma therapy.