Hematopoietic Stem Cell Mobilization ; methods ; Humans ; Peripheral Blood Stem Cell Transplantation ; methods

Objective To compare medical students' learning curve between Macintosh laryngoscope and optical laryngoscope in endotracheal intubation and to decide which one is more suitable for novice personnel.Methods Totally 26 interns after being trained by experienced anesthesiologists were enrolled in this study.They performed intubation on 6 patients by using both Macintosh laryngoscope and optical laryngoscope ( each laryngoscope for 3 patients) under the supervision of experienced anesthesiologists.The sequence of laryngoscope was determined by computer-generated random number list.Results Totally 149 patients who were ready to receive surgery under general anesthesia were enrolled in this randomized crossover controlled study.Duration of intubation was significantly shorter in Airtraq group (78 -± 33 ) s than in Macintosh group (114 ± 32) s,P ＜ 0.001.Intubation success rate was significantly higher in Airtraq group than in Macintosh group ( 87.8％ vs.66.7％,P ＜ 0.05 ).Conclusions With the advantages of rapid learning curve,higher intubation success rate and shorter intubation duration,Airtraq laryngoscope is easier to master for novice personnel.

Hemoglobinuria, Paroxysmal ; therapy ; Humans

Anemia, Aplastic ; diagnosis ; Consensus ; Humans

Animals ; Coxsackievirus Infections ; metabolism ; Enterovirus B, Human ; Female ; Male ; Mice ; Myocarditis ; metabolism ; Myocardium ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

Accidents, Occupational ; Bromine ; poisoning ; Humans ; Hydrobromic Acid ; poisoning

Animals ; Area Postrema ; physiology ; Blood Pressure ; Electric Stimulation ; Electrocardiography ; Male ; Neurotransmitter Agents ; Peroneal Nerve ; physiology ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo explore the effect of 3-n-butylphthalide pretreatment on the delayed neuronal death(DND) and the expreesion of heat shock protein70 (HSP70) in rat hippocampus after ischemia/ reperfusion.

METHODSAll rats were randomly divided into sham group (n = 36), total cerebral ischemia (TCI) group (n = 36), butylphthalide (NBP) group (n = 6), NBP + TCI group( n = 36), quercetin + NBP + TCI group (n = 6), dimethyl sulfoxide (DMSO) + NBP + TCI group (n = 6). The model of total cerebral ischemia/reperfusion was established by blocking vertebral arteries and carotid arteries. In sham group, TCI group and NBP group, the animals were further divided into instantly, 6 h, 12 h, 1 d, 3 d, 5 d groups according to the time interval after sham operation or TCI. Histological changes of the hippocampus were evaluated using thionin staining under light microscope by determining the delayed neuronal death (DND) and the expression of HSP70 was assayed using immunohistochemistry.

RESULTSNBP pretreatment could reduce delayed neuronal death in CA1 of hippocampus induced by TCI-reperfusion injury in rats, and up-regulated the expression of HSP70 in CA1 hippocampus of brain ischemic/reperfusion for 5 days. Quercetin blocked the acquirement of the brain ischemic tolerance induced by NBP preconditioning.

CONCLUSION3-n-butylphthalide (NBP) prevents the neurons from ischemia/reperfusion injury through upregulating the expression of HSP70.

Animals ; Benzofurans ; pharmacology ; CA1 Region, Hippocampal ; cytology ; pathology ; Cell Death ; Cerebral Infarction ; drug therapy ; HSP70 Heat-Shock Proteins ; metabolism ; Ischemic Preconditioning ; Neurons ; cytology ; Rats ; Rats, Wistar ; Reperfusion Injury ; drug therapy

Objective To evaluate the implementation effect of Malaria Elimination Project supported by the Global Fund in Shaanxi Province so as to provide the evidence for the scientific implementation of Malaria Elimination Action Plan and the exami-nation and evaluation work. Methods The data of malaria prevention and control work were collected and analyzed statistically in 27 counties from 2010 to 2012 in order to evaluate the measures and effects of malaria control. Results There were 64 reported cases in the project areas from 2010 to 2012,and the average annual incidence rate was 0.15/10 million with a declining trend. The infected area sources of patients were mainly imported overseas accounting for 79.69%of the total cases. The blood completion rate of febrile patients was 94.14%,and the scope of the blood smears testing work had expanded to 88 counties(including 61 third-class counties). The training coverage rate of malaria prevention knowledge and skills had achieved to 100%. The awareness rates of malaria prevention and control were 91.68%and 89.44%in the students and local residentsrespectively. The case reporting with-in 24 h after diagnosed,epidemiological case study and outbreak disposal,laboratory testing and confirming and the other case management capabilities had also improved significantly. Conclusions The implementation of the Global Fund Malaria Project in Shaanxi Province has accelerated the process of Malaria Elimination work and improved significantly the malaria control ability in the project areas.

Objective To compare the effects of flurbiprofen axetil and fentanyl of postoperative analgesia on T-lymphocytes subtypes in patients undergoing esophagectomy.Methods Forty patients undergoing esophagectomy were randomly divided into two groups(20 cases each):group C (group fentanyl) was given fentanyl 20μg/kg plus tropisetron 5 mg diluted to 100 ml via PCIA after surgery,group F (group flurbiprofen-fentanyl) was administrated flurbiprofen axetil 50 mg at 30 min before the end of surgery,fentanyl 10μg/kg and flurbiprofen axetil 100 mg plus tropisetron 5 mg diluted 100 ml was administrated via PCIA after surgery.The PCIA rate was 2 ml/h,bolus 0.5ml,lock time 15 min.The VAS score was recorded at 12,24,48 h after surgery.Blood samples 2 ml were obtained from peripheral vein for determination of CD3+,CD4+,CD8+ and CD4+/CD8+ at 30 min before surgery(T0),24 h(T1)and 72 h(T2)after surgery.Results Patients in two groups did not show any significant difference in the VAS scores(P＞0.05).At T1 CD3+,CD4+ T-lymphoeytes were significantly lower than those at T0 in two groups(P＜0.05).At T2,CD3+,CD4+ T-lymphocytes in group F were significantly higher than those in group C(P＜0.05).In group C,CD3+,CD4+ T-lymphocytes at T2 were significantly decreased than those at T0(P＜0.05).CD8+ T-lymphocytes Was no significantly changed in two groups and at each time point determined (P＞0.05).Conclusion Postoperative analgesia by using flurbiprofen axetil and fentanyl can diminish the using dose of postoperative opioid drug,and it can'improve patient's cellular immune function.