The vaseularization of tissue-engineered bone is the key problem which the development and employment of large sized tissue-engineered bone.The vascular endothelial cell has a great effect on promoting vascularization in tissue-engineered bone.Vascularizations fall into two modes of vaseulogenesis and angiogenesis according to differences in source of endothelial cells.Co-culture of osteoblasts and vascular endothelial cells has better result than single culture of each kind of cells.Different ways of improving the vascularization,such as searching for new source of vascular endothelial cell,co-culture and in vivo experiment are investigated to meet the challenge of bone tissue engineering.

BACKGROUND: It has been verified vascular endothelial cells can propagate and differentiate into vessel by planted in the tissue-engineered material in vivo, but it may cause severe trauma when obtain vascular endothelial cells, and it has limited sources. OBJECTIVE: To investigate whether bone marrow mesenchymal stem cells (BMSCs) can differentiate into vascular endothelial cells by combined induction of vascular endothelial cell growth factor (VEGF), basic fibroblast growth factor (bFGF) and insulin-like growth factor (IGF)-1 by experimental observation. DESIGN, TIME AND SETTING: The control cell observation experiment was conducted at the Central Laboratory of First Affiliated Hospital, Liaoning Medical University from November 2006 to June 2007. MATERIALS: An 8-week-old Japanese big ear rabbit was used to isolate BMSCs. VEGF, bFGF and IGF-1 were purchased from Peprotech Company. METHODS: After anaesthesia, bone marrow was extracted from the rabbit. Enough BMSCs were harvested by adherence and trypsinization and randomized into two groups. BMSCs in the induction group were inoculated in DMEM supplemented with 10% fetal bovine serum (FBS), 10 ?g/L VEGF, 1 ?g/L bFGF and 2 ?g/L IGF-1 for 2 weeks. BMSCs in the control group were inoculated in DMEM containing 10% FBS. MAIN OUTCOME MEASURES: Morphological observation, nitric oxide (NO) content detected, immunohistochemistry staining of Ⅷ factor-related antigen and Weibel-Palade body under a electron microscope in BMSCs. RESULTS: Five days after induction, BMSCs were distributed in cluster, showing round. Non-induced BMSCs were evenly distributed, showing spindle or triangle. Fourteen days after induction, NO content in supernatant was significantly higher in the induction group than in the control group (t=3.75, P

Tuina is a physical therapy for treatment and prevention of diseases.The predecessors had summed up the systematic tuina manipulations through experiences.In order to study the scientificity and usability of the technology,the researchers established a mathematical model of tuina manipulations,and used video technology to capture the trajectory of the manipulations.Using the mechanical sensor to sense the real manipulations,researchers developed a tuina manipulation instrument and obtained a lot of basic mechanics data about the manipulation technology.Through the summary of the research results of the predecessors,accurate,true and comprehensive mechanical parameters of technology of tuina manipulations were obtained to guide the research and development of instruments of tuina manipulations,and promote the development of the discipline of tuina science.

Objective To analyze the effect of periodic physical examination on improvement of health behaviors of elderly people. Methods 186 elderly people from June 2010 to June 2011 were chosen.The observation group (93 people) was taken with regular health checkup every six months.The control group (93 people) did not receive regular health checkup.The health promoting lifestyle scale (HPLP) and the knowledge and compliance behavior questionnaire of residents were adopted for investigation and analysis. Results The total score for health behaviors in the observation group was significantly higher than the control group.The score of six indicators of HPLP,including self-actualization,health resporsibility,physical activity,nutrition,pressure regulator and interpersonal relationship were higher than the control group,the differences between the two groups were significant.The average score for health knowledge of the residents for the observation group was (44.52 ± 2.30) points,and the compliance behavior score was (41.21 ± 2.80) points.The scores were siguificantly higher than the control group,the difference was significant.Conclusions The periodic health examination for elderly people has a positive effect in improving their health behavior.

Dental Porcelain ; classification ; Humans

Anti-Bacterial Agents ; pharmacokinetics ; pharmacology ; Cell Membrane ; drug effects ; Microbial Sensitivity Tests

AIM: To investigate the enhancement of ethanol on percutaneous permeation and topical anaesthesia of tetracaine gel. METHODS: Tetracaine (4%, w/w) gels were prepared by using 20% and 70% ethanol as penetration enhancers. Franz diffusion chamber and UV spectrophotometry were adopted in the transdermal osmotic test of isolated mouse skin. Von Frey test was used to evaluate the topical anesthetic effect of tetracaine gels. RESULTS: 20% and 70% ethanol greatly improved the percutaneous permeation of tetracaine gel (P

The changes of[Ca~(2+)]and metabolism of lipid in human monocyte-macrophages as inter- acted with glycosylated LDL(glc-LDL)were observed.The intracellular[Ca~(2+)]was higher than that of LDL(P

Objective To study the ability of adhesion, proliferation and differentiation of human adipose mesenchymal stern cells,which were cultured in PLGA,and thus to provide a basis for their in vivo implantation.Methods PLA was mixed with PGA in a ratio of 1∶ 1,and PLGA was obtained by insert moldling with organic solvents.In vitro, human adipose mesenchymal stem cells were adhered to PLGA.The attachment and the proliferation of human adipose mesenchymal stem cells in PLGA were analysed by scanning electron microscope,and the morphological change of the cells was investigated before and after induction.Results Human adipose mesenchymal stem cells were able to attach, proliferate and secrete extracellular matrix in porous PLGA (pore size:100～400 pm) with porosity of 85 %, and to differentiate into round lipoblast-like cells under the induction of adipose medium.After 14 days, the particulate of PLGA was overlaid by extracellular matrix .Conclusion Human adipose mesenchymal stem cells have the capacity to adhere to PLGA and proliferate, indicating that PLGA can be used as a carrier for human adipose mesenchymal stem cells in adipose tissue engineering.

Objective To assess the clinical effect of Novonorm on treating type 2 diabetic patients with atherosclerosis. Methods Sixty-five patients of type 2 diabetes with atherosclerosis were randomly divided into 2 groups :36 patients took Novonorm,and the other 29 individuals were treated with Glipizide. The blood glucose and CIMT were compared before and after treatment for 12 months. Results The postprandial blood glucose decreased by(1.99 ± 1.06)mmol/L after the treatment of Novonorm,which was significantly higher than that of(0. 99 ±0. 54)mmol/L after the treatment of Glipizide(P ＜ 0. 05). CIMT significantly decreased from (1.11 ± 0. 07)mm before treatment to(1.02 ± 0. 08)mm after treatment in the Novonorm group(P ＜ 0. 05),whereas CIMT were similar between after and before treatment in the Glipizide group(1.07 ± 0. 06)mm vs.(1.10 ± 0. 08)mm(P ＞ 0. 05).Conclusions Novonorm is a kind of effective and safe regent,which can be used to treat type 2 diabetic patients with atherosclerosis.