BACKGROUND: It has been verified vascular endothelial cells can propagate and differentiate into vessel by planted in the tissue-engineered material in vivo, but it may cause severe trauma when obtain vascular endothelial cells, and it has limited sources. OBJECTIVE: To investigate whether bone marrow mesenchymal stem cells (BMSCs) can differentiate into vascular endothelial cells by combined induction of vascular endothelial cell growth factor (VEGF), basic fibroblast growth factor (bFGF) and insulin-like growth factor (IGF)-1 by experimental observation. DESIGN, TIME AND SETTING: The control cell observation experiment was conducted at the Central Laboratory of First Affiliated Hospital, Liaoning Medical University from November 2006 to June 2007. MATERIALS: An 8-week-old Japanese big ear rabbit was used to isolate BMSCs. VEGF, bFGF and IGF-1 were purchased from Peprotech Company. METHODS: After anaesthesia, bone marrow was extracted from the rabbit. Enough BMSCs were harvested by adherence and trypsinization and randomized into two groups. BMSCs in the induction group were inoculated in DMEM supplemented with 10% fetal bovine serum (FBS), 10 ?g/L VEGF, 1 ?g/L bFGF and 2 ?g/L IGF-1 for 2 weeks. BMSCs in the control group were inoculated in DMEM containing 10% FBS. MAIN OUTCOME MEASURES: Morphological observation, nitric oxide (NO) content detected, immunohistochemistry staining of Ⅷ factor-related antigen and Weibel-Palade body under a electron microscope in BMSCs. RESULTS: Five days after induction, BMSCs were distributed in cluster, showing round. Non-induced BMSCs were evenly distributed, showing spindle or triangle. Fourteen days after induction, NO content in supernatant was significantly higher in the induction group than in the control group (t=3.75, P

The vaseularization of tissue-engineered bone is the key problem which the development and employment of large sized tissue-engineered bone.The vascular endothelial cell has a great effect on promoting vascularization in tissue-engineered bone.Vascularizations fall into two modes of vaseulogenesis and angiogenesis according to differences in source of endothelial cells.Co-culture of osteoblasts and vascular endothelial cells has better result than single culture of each kind of cells.Different ways of improving the vascularization,such as searching for new source of vascular endothelial cell,co-culture and in vivo experiment are investigated to meet the challenge of bone tissue engineering.

Tuina is a physical therapy for treatment and prevention of diseases.The predecessors had summed up the systematic tuina manipulations through experiences.In order to study the scientificity and usability of the technology,the researchers established a mathematical model of tuina manipulations,and used video technology to capture the trajectory of the manipulations.Using the mechanical sensor to sense the real manipulations,researchers developed a tuina manipulation instrument and obtained a lot of basic mechanics data about the manipulation technology.Through the summary of the research results of the predecessors,accurate,true and comprehensive mechanical parameters of technology of tuina manipulations were obtained to guide the research and development of instruments of tuina manipulations,and promote the development of the discipline of tuina science.

Objective To analyze the effect of periodic physical examination on improvement of health behaviors of elderly people. Methods 186 elderly people from June 2010 to June 2011 were chosen.The observation group (93 people) was taken with regular health checkup every six months.The control group (93 people) did not receive regular health checkup.The health promoting lifestyle scale (HPLP) and the knowledge and compliance behavior questionnaire of residents were adopted for investigation and analysis. Results The total score for health behaviors in the observation group was significantly higher than the control group.The score of six indicators of HPLP,including self-actualization,health resporsibility,physical activity,nutrition,pressure regulator and interpersonal relationship were higher than the control group,the differences between the two groups were significant.The average score for health knowledge of the residents for the observation group was (44.52 ± 2.30) points,and the compliance behavior score was (41.21 ± 2.80) points.The scores were siguificantly higher than the control group,the difference was significant.Conclusions The periodic health examination for elderly people has a positive effect in improving their health behavior.

[Objective]To describe the clinical applycation of domelike decompression of the lumbar vertebral canal and evaluate the outcomes.To evaluate the effects on lumbar stability of domelike decompression through biomechanical canal study.[Method]Domelike decompress of the lumber vertebral canal were performed in 197 patients,with an average age of 52.7 and an average history of 6 years and 8 months,The surgicall outcomes were evaluated with modify Japanese Orthopedic Association Low Back Pain Score(M-JOA).Biomechanical study were performed on lumbar specimens from thirty fresh porcine model which were divided into three groups,Group A(n=10)was biomechanically tested after simulated laminectomy decompression,Group B(n=10)was biomechanically tested after simulated domelike decompression,Group C(n=10)was biomechanicaliy tested on intact,All were tested in flexion extension,torsion,and lateral bending and axial rotation moments as well as axial compressive loads.Load deflection curves were obtained each time,and stiffness values were calculated from the curves,Differences were checked for significance(P

Anti-Bacterial Agents ; pharmacokinetics ; pharmacology ; Cell Membrane ; drug effects ; Microbial Sensitivity Tests

Objective:To investigate the remodeling of alveolar bone and the changes of roots after an-terior retraction with maximum anchorage by analyzing CBCT data from adult cases.Methods:The sam-ple comprised 48 incisors and 24 canines from 12 patients (18 to 40 years of age)with problems of ma-xillary protrusion or upper arch protrusion.CBCT scans were exposed before and after treatment,and la-teral cephalometric images as well as multiple planar reconstruction images were reconstructed.Tracing superimpositions of sagittal sections and three-dimensional reconstructions were done for qualitative analy-sis.For all maxillary anterior teeth,changes of root length,alveolar bone height and labial-palatal thick-nesses at different levels were evaluated.The average of measurements taken by the same tester in three times was processed by SPSS 17.0 statistical package.Results:In 6 of the 12 cases,alveolar thickness became thinner on labial side [apical area:(-0.64 ±1.18)mm]while thicker on palatal side [apical area:(0.93 ±2.0)mm]and the root length decreased[(-0.95 ±0.79)mm].In the other 6 cases, the incisors’alveolar bone became thicker on labial side [apical area:(2.12 ±1.46)mm]while thin-ner on palatal side [apical area:(-2.88 ±0.58)mm]and the loss of root length was obvious[(-2.12 ± 1.43)mm].In all the 12 cases,the canines’alveolar bone became thinner on labial side especially on the apical level [(-0.27 ±1.86)mm]while greatly thicker on palatal side [apical area:(6.40 ± 6.00)mm]and the root resorption was slight [(-1.12 ±1.19)mm].For all the anterior teeth,the height of alveolar bone reduced around them after retraction.Conclusion:When the root apical moved more palatally,more root resorption would occur and the alveolar bone would get thicker on labial side but thinner on palatal side and thinner as a whole after anterior retraction with maximum anchorage.In the vertical direction,the height of the alveolar bone generally decreased on all sides and decreased the most on the palatal side.

OBJECTIVETo analyze the asymmetry of measurements in facial asymmetry patients with skull positioning posterior-anterior radiographs after orthodontic-orthognathic treatment.

METHODSPosterior-anterior cephalometric radiography and cephalometric analysis were performed in forty-five patients with different degrees of facial asymmetry. A single sample t-test was conducted to compare the asymmetry of measurements before treatment and individual normal occlusion. The measurement and reference values in facial asymmetry patients who underwent orthodontic-orthognathic treatment were summated by analyzing the correlation coefficient. The paired t-test was employed to compare the difference between the two groups before and after the treatment.

RESULTSFourteen measurements were significantly different before the treatment and individual normal occlusion (P < 0.05). Me[X] exhibited a higher correlation index of Ag[X] in forty-five cases. Twenty-eight patients showed the main asymmetry in the mandibular body, whereas fifteen patients in the ramus. Two patients showed the main asymmetry in both mandibular body and ramus. Fifteen measurements were significantly different before and after the treatment (P < 0.05).

CONCLUSIONFacial asymmetry is mainly concentrated in one-third of the surface, primarily manifested in the mandibular body. Orthodontic-orthognathic treatment is preferred when Ag[X] is greater than the minimum reference value of 11.31%, Go[X] is greater than 9.79%, and Me[X] is greater than 5.2 mm.

Dental Porcelain ; classification ; Humans

Objective To study the ability of adhesion, proliferation and differentiation of human adipose mesenchymal stern cells,which were cultured in PLGA,and thus to provide a basis for their in vivo implantation.Methods PLA was mixed with PGA in a ratio of 1∶ 1,and PLGA was obtained by insert moldling with organic solvents.In vitro, human adipose mesenchymal stem cells were adhered to PLGA.The attachment and the proliferation of human adipose mesenchymal stem cells in PLGA were analysed by scanning electron microscope,and the morphological change of the cells was investigated before and after induction.Results Human adipose mesenchymal stem cells were able to attach, proliferate and secrete extracellular matrix in porous PLGA (pore size:100～400 pm) with porosity of 85 %, and to differentiate into round lipoblast-like cells under the induction of adipose medium.After 14 days, the particulate of PLGA was overlaid by extracellular matrix .Conclusion Human adipose mesenchymal stem cells have the capacity to adhere to PLGA and proliferate, indicating that PLGA can be used as a carrier for human adipose mesenchymal stem cells in adipose tissue engineering.