Objective: To study the effect of different processing methods and conditions for bioactive constituents in Polygoni Multiflori Radix (the root tubers of Polygonum multiflorum) and their change regularity, in order to optimize and establish appropriate drying methods and conditions. Methods: The stilbene glucoside, free-anthraxquinones, combined-anthraxquinones, and catechin from 12 kinds of Polygoni Multiflori Radix in different processing methods were determined by MEKC-DAD. The multiple functional substances of principal component analysis (PCA) was made to standardized treatment for the comprehensive evaluation of the 12 samples by different drying methods. Results: The contents of multiple functional substances in different processed products from Polygoni Multiflori Radix presented the certain regularity. The contents of stilbene glucoside and combined anthraquinone were the highest in Polygoni Multiflori Radix which was cut into thick pieces dried in the sun, the total free anthraquinones were the highest in Polygoni Multiflori Radix which was cut into thick pieces dried at 70℃, the content of catechin was the highest in Polygoni Multiflori Radix which was cut into thin pieces dried at 40℃. The comprehensive evaluation index obtained with PCA showed that the sample cut into thick pieces dried in the sun was significantly higher than those with other samples. Conclusion: The influence of processing methods by the determination of multiple functional substances in Polygoni Multiflori Radix has been investigated and a scientific basis for the suitable processing method of Polygoni Multiflori Radix provided.

Objective: To establish a method for simultaneous analysis on the inorganic elements in Polygoni Multiflori Radix. by inductively coupled plasma mass spectrometry (ICP-MS) and compare the inorganic elements in Polygoni Multiflori Radix between different origin and commercial herbs. Methods: The sample solutions were analyzed by ICP-MS after microwave digestion. The data of correlations, principal components, and cluster were analyzed with the SPSS 16.0 software. Results: Twenty four inorganic elements in Polygoni Multiflori Radix were determined. And there were some correlations among the inorganic elements in Polygoni Multiflori Radix. The contents of K, Fe, Mg, Ca, and P were abundant. The content of heavy metals and harmful elements should be caused for concern. Six main factors were selected by principal component analysis (PCA). The PCA results showed that Fe, Si, Ca, Al, K, Be, Sb, Mn, Zn, and Ba may be the characteristic elements in Polygoni Multiflori Radix. Conclusion: This experiment provides the evidence for the quality control and safety evaluation of Polygoni Multiflori Radix.

OBJECTIVE: To establish a micellar electrokinetic chromatography with diode array detection (MEKC-DAD) method for simultaneous determination of seven components including stilbene glucoside, emodin, aloe-emodin, rhein, chrysophanol, physcion, and catechin in Polygoni Multiflori Radix, of different origins and commercial herbs. METHODS: Based on the mode of MEKC, an uncoated fused silica capillary (75 μm × 64.5 cm, 56 cm of effective length) was adopted, and 25 mmol · L^-1 borax-30 mmol · L^-1 SDS-10% acetonitrile (pH 9.60) was selected for the running buffer. The detection wavelength was set at 254 nm. The separation voltage was 25 kV and the column temperature was maintained at 24℃. The sample was injected at 5 kPa for 5 s. RESULTS: The calibration curves of the seven components in Polygoni Multiflori Radix showed good linearity (r ≥ 0.9994). The average recoveries of the method were between 99.164%-101.504%. CONCLUSION: The established method is reliable and accurate, and can be used for the quality control of Polygoni Multiflori Radix.

OBJECTIVE: To explore the dynamic changes of the inorganic elements in Polygoni Multiflori Radix.

Objective To investigate the clinical effect of sodium hyaluronate combined with glucocorticoid on the treatment of periarthritis of shoulder.Methods106 patients in department of orthopedics, the second hospital of Ningbo with periarthritis of shoulder were randomly divided into 2 groups (n=53).The control group were treated with anti-inflammatory and analgesic treatment, at this basis, the study group were treated with sodium hyaluronate combined with Glucocorticoid.5 weeks for a course of treatment.Levels of serum inflammation, laboratory-related indicators were compared.ResultsCompared with before treatment, shoulder joint activity in the two groups increased, VAS score decreased, the scores of strength of shoulder joint, range of motion and daily activities increased, levels of serum IL-6, IL-10, TNF-α decreased(P<0.05).Compared with the control group, scores of activity in the study group were higher, VAS scores were lower, scores of strength of shoulder joint, range of motion and daily activities were higher, levels of serum IL-6, IL-10, TNF-α were lower(P<0.05).The effective rate in the control group(73.59%) was lower than the study group(90.57%), but there was no statistical difference.ConclusionThe effect is accurate which sodium hyaluronate combined with glucocorticoid was used in the treatment of periarthritis of shoulder, and it can reduce the inflammation index and improve the joint activity.

Objective: To analyze the change of chemical constituents of Polygoni Multifori Radix from different harvest time. Methods: Fifteen batches of Polygoni Multifori Radix samples from different harvest time were determined by UPLC-Triple TOF-MS/MS. Through the analysis of the multistage tandem mass spectrometry, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, and noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing. The components were identified according to a mass spectrometry accurate mass and two mass spectrometry fragmentation information, combined with the software of database search and literature. Results: The chemical composition in Polygoni Multifori Radix samples from different harvest time are clearly distinguished. Twenty-four kinds of differential chemical constituents were identified. Among them, there are nine kinds of common differential constituents which presented different changing laws. Conclusion: This study provides the basic data for revealing the dynamic change law of metabolite accumulation of Polygoni Multifori Radix and confirms its optimal harvest period.

A total of 526 team members from 28 community health service (CHS) centers in 5 pilot cities were surveyed by a questionnaire.All CHS centers developed team service.And 275 service teams were established and the coverage rate of area resident committee was 100％.The teaming modes were mainly divided into four kinds:medical-nursing-prevention classic combination,big team professional expansion,small team medical combination and non-health technology extensive participation.The team services showed multiple advantages of professionism,energy and time complementing.We should strengthen team construction,improve perfect team service mode and policy support and create a good environment for CHS development.

Objective To investigate the role of NMDA receptors in central medial thalamus (CMT) in the unconscious?ness induced by general anesthesia. Methods A total of 60 rat models for microinfusion were assigned into 4 groups (n=15 for each group). After induction with propofol, 10 mmol/L (NMDA10 group), 20 mmol/L (NMDA 20 group) and 40 mmol/L (NMDA40 group) of NMDA and normal saline (group C) with equal volume were microinfused into CMT. The incidence of purposeful movement and recovery time of righting reflex were observed in each group respectively. Infusion sites were local?ized by histological method. Results When the microinfusion site localized within CMT, comparing with group C, the recov?ery time of righting reflex reduced notably in three NMDA groups (P<0.05). The recovery time was significantly shorter in NMDA20 group and NMDA40 group than that of NMDA10 group. The incidence of purposeful movement during propofol an?esthesia was higher in NMDA20 group and NMDA40 group than that of group C (P<0.05). When the microinfusion site lo?calized out of CMT, the recovery time of righting reflex was remarkably longer than that within CMT in three NMDA groups (P<0.05), and there was no significant difference in the incidence of purposeful movement and recovery time between four group (P>0.05). Conclusion Microinfusion of NMDA agonist into CMT reverses propofol anesthesia, indicating that NMDA receptor in CMT may contribute to the propofol-induced unconsciousness.

Objective:To evaluate the vomer development of cleft palate patients.Methods:38 patients over 1 4 years(averaged 23.4 years)of age with cleft palate and 76 controls of normal people(aged 22.8 year on average)were included.The 3D computed tomo-graphy reconstruction images of the bony nasal septum were measured.The development of the vomer was evaluated by comparing the L1 (the length of the lower edge of the vomer),L2 (the length from nasal spine to the point of the sella)and S (the approximate area of vomer)among deferent groups.Results:The L1 ,L2,and S of cleft palate patients were smaller than those of the controls(P <0. 05).Compared with the postoperative cleft cases,the S and L2 of preoperative cases were bigger(P <0.05).Conclusion:The vomer development is adversely affected by cleft palate.Not only the vomer-palate fusion is lower,but also the sutura between vomer and na-sal septum cartilage and ethmoid bone are short.And the latter is greatly influenced by surgical trauma.

Objective To analyze the epidemiological characteristics of overseas imported malaria reported in Chongqing City from 2011 to 2015,so as to provide the evidence for improving the imported malaria control strategies. Methods The epi-demiological data of overseas imported malaria cases were collected and analyzed descriptively for the species,original coun-tries,diagnosis and treatment in Chongqing City from 2011 to 2015. Results A total of 148 overseas imported malaria cases were reported in Chongqing City from 2011 to 2015,in which 96(65.54%)cases were falciparum malaria,and 37(24.32%) cases were vivax malaria;125(84.46%)cases were infected in Africa,and the rest of 23(15.54%)cases were infected in Southeast Asia. Most of the patients were 30 to 50 years old male workmen,and the ratio of male to female was 11.42:1. There was no obvious seasonal distribution among the reported timelines of the cases;however,there were two small peaks from June to August and from January to February. The median interval time of imported malaria cases from malaria onset to see a doctor was 1 day and from seeing the doctor to get malaria diagnosis was 2 days. The patients'first selected institutions were county medical institutions(50 cases,33.78%),then provincial medical institutions(36 cases,24.325%)and private doctors(20 cases,13.51%),and only 79(53.38%)patients got malaria diagnoses in their first selected institutions. The standard treatment were provided to 142(95.54%)cases. There were 43(29.05%)patients had serious complications and three patients were dead. Conclusion It is very important to enhance the multi-sector's collaboration to establish the collaborative investigation mechanism for screening malaria patients,and strengthen malaria health education for overseas workers and training courses in primary care medical institutions.