1.Capillary Electrophoresis of Neurotransmitters Under the Control of Radial Electric Field
Chinese Journal of Analytical Chemistry 2001;29(6):661-663
Separations of neurotransmitters such as dopamine (DA), ser otonin (5-HA), norepinephrine (NE) and epinephrine (E) were performed successf ully using a homemade electric field modulated capillary electrophoretic system, which could offer both radial and axial electric fields with only one high volt age power supply. DA and 5-HT were eluted simulaneously and could not be resolv ed in 0.01 mol/L phosphate buffer at pH 2.5. Alcohol additives, such as methanol , ethanol or 1-propanol were added to the buffer to change the solvation shell of the solutes, which changed their effective sizes and electrophoretic mobiliti es of the solutes accordingly. The optimum composition was a buffer of 20% (V /V) 1-propanol, with resulted resolutions 0.74 (DA/5-HT), 0.56(5-HT/NE) and 0.77 (NE/E). If a positive radial voltage of 6.6 kV was applied, the resolut ions were improved to 1.48, 0.71 and 1.32, respectively.
2.Analysis of Non-irrational Drug Use in the Department of Cardiovascular Diseases
China Pharmacy 1991;0(05):-
OBJECTIVE: To evaluate the irrational use of drugs in the department of cardiovascular diseases.METHODS: The irrational use of drugs in the department of cardiovascular diseases from Nov,2007 to Aug 2008 was analyzed statistically.RESULTS: Of the total 194 inpatients(1 745 times in total) investigated,irrational use of antibiotics were found in 118 cases,accounting for 60.82% of the total inpatients or 6.76% in the total 1 745 times;26 cases involved irrational perioperative use of antibiotics,accounting for 1.49% of the total 1 745 times;76 cases had irrational use of non-antibiotics,accounting for 39.18% of the total inpatients or 4.36% of the total 1 745 times.The irrational drug use in the cardiovascular department witnessed an improvement ever since Feb 2008 when the full-time clinical pharmacists began their work in this department.CONCLUSION: The clinical pharmacists can enhance the clinical rational drug use level by summarizing experience and carrying out pharmaceutical care accordingly.
3.Emergence agitation after total intravenous anesthesia for maintenance with remifentanil in children with OSAS
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(10):-
Objective To compare the effects of total intravenous anesthesia(TITA) by using remifentanil combined with propofol and intravenous-inhaled anesthesia by using propofol combined with sevoflurane on emergence agitation(EA) in children undergoing surgery of obstruction sleep apnea syndrome(OSAS). Methods Forty children of 3 to 7 years old scheduled for elective surgery of OSAS were randomly divided into TITA group(group T,n=20) and combined anesthesia group(group C,n=20).Patients in group T were induced with remifentanil 1 ?g/kg,midazolam 0.2 mg/kg,propofol 2.5 mg/kg and rocurolnium 0.6 mg/kg and maintained with remifentanil(0.4-0.5 ?g?kg-1?min-1) and propofol(4-6 mg?kg-1?h-1) until the end of the operation.Patients in group C were induced with midazolam 0.2 mg/kg,propofol 2.5 mg/kg and rocurolnium 0.6 mg/kg and maintained with propofol 4-6 mg?kg-1?h-1 and sevoflurane inhalation(1.2-1.4 MAC) until the end of the operation.EA was evaluated by 5-point scoring scale. Results The score of EA was significantly lower in group T than that in group C(P
5.Analysis and discussion on current condition of acupuncture clinical research registration.
Chinese Acupuncture & Moxibustion 2015;35(6):605-607
To introduce the international registration condition of acupuncture clinical research. With the examples of World Health Organization International Clinical Trials Registry Platform and the U. S. National Institutes of Health Clinical Registration Platform, the registration method and current condition of acupuncture clinical trials in international clinical trials registration platform were analyzed. The results indicate that the number of acupuncture clinical trials registration is gradually increased and the registration number from China is on the rise as well. But most domestic acupuncture clinical researches haven't been registered arid the researchers' valuing degree for clinical trials registration and methodology research needs to be improved.
Acupuncture Therapy
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standards
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Biomedical Research
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legislation & jurisprudence
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standards
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Clinical Trials as Topic
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legislation & jurisprudence
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standards
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Humans
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Registries
6.Effects of different concentrations of ox-LDL on the proliferation of rat theca cells and the expression of steroidogenesis related genes LXR-α and StAR
Ying CHEN ; Yi ZHANG ; Cong LI
Journal of Shanghai Jiaotong University(Medical Science) 2017;37(3):318-324
Objective · To investigate the effects of ox-LDL on the proliferation of rat theca cells and expression of LXR-α and StAR, two genes associated with androgen biosynthesis. Methods · The expression of LXR-α in the ovarian tissue of rats was determined by immunohistochemistry. Primary theca cells were isolated and collected from rat ovary and cultured in vitro. Furthermore, the theca cells were treated with 25, 50, 100, 150, 200, 300 and 400 mg/L ox-LDL, respectively. The variations in LXR-α mRNA were identified using real-time PCR. MTT assay was performed to detect cell viability. The expression of LXR-α and StAR was measured by Western blotting analysis. Results · The effect of ox-LDL on the proliferation of rat theca cells and the levels of LXR-α and StAR in theca cells was in a concentration-dependent manner. Following exposure to various concentration of ox-LDL for 24 h, the proliferation of theca cells was induced by low concentration of ox-LDL (25-150 mg/L), and 100 mg/L ox-LDL showed the most significant inducing effect. Moreover, the cell survival rate was diminished considerably following with ox-LDL concentration increasing, especially lowered by 400 mg/L ox-LDL. The mRNA level of LXR-α was increased with low concentration of ox-LDL (25-150 mg/L) and the impact of ox-LDL on the induced expression of LXR-α mRNA was considerably distinct at the concentration of 150 mg/L. On the other hand, the expression of LXR-α mRNA was reduced with high concentration of ox-LDL, and the impact of 400 mg/L ox-LDLwas substantially distinct. The protein expression levels of LXR-α and StAR were increased with 150 mg/L ox-LDL, but StAR protein level in 150 mg/L ox-LDL group revealed no significant difference when compared with control group. The expression of LXR-α and StAR protein was significantly inhibited with 400 mg/L ox-LDL in the rat theca cells. Conclusion · Low concentrations of ox-LDL can induce the proliferation of theca cells, and promote the expression of StAR and LXR-α. Whereas, high concentrations of ox-LDL can reduce the cell viability and inhibit the expression of StAR and LXR-α.
7.Study on identification of Sarcandra glabra and Chloranthus spicatus's leaves by PCR amplification of specific alleles.
Yi-cong WEI ; Ying CHEN ; Lin-quan LUO ; Qun-xiong YANG ; Yi-Juan CHEN ; Yi-chi LIANG ; Su-Rong CHEN
China Journal of Chinese Materia Medica 2014;39(17):3259-3262
The paper is aimed to identify SNP in Sarcandra glabra and Chloranthus spicatus, and authenticate S. glabra from Ch. spicatus and the mixture by using PCR amplification of specific alleles. SNPs in the ITS sequences of S. glabra and Ch. spicatus were found by ClustulX 2. 1 program and Bioedit software. Primers for authentic S. glabra and Ch. spicatus was designed according to the SNP site, and ITS sequence universal primers plus to the authentic primer to construct a multi-PCR reaction system, and then optimized the PCR reaction system. Five hundred and eighty band special for S. glabra and 470 bp band special for Ch. spicatus were found by using multi-PCR reaction. The multi-PCR reaction system could be applied to identify S. glabra and Ch. spicatus's leaves.
DNA, Plant
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analysis
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genetics
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DNA, Ribosomal
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genetics
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DNA, Ribosomal Spacer
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analysis
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genetics
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Magnoliopsida
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classification
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genetics
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Plant Leaves
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genetics
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Polymerase Chain Reaction
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Polymorphism, Single Nucleotide
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RNA, Ribosomal
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genetics
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RNA, Ribosomal, 18S
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genetics
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RNA, Ribosomal, 5.8S
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genetics
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Species Specificity
8.Protection effect of lactobacillus with selenium enrichment on growth and lymphocyte transformation of rats with liver injuries.
Yi-Yung SUN ; Long CHEN ; Ying-Zi JIANG
Chinese Journal of Applied Physiology 2003;19(4):366-397
Animals
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Female
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Lactobacillus
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Liver
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pathology
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Lymphocyte Activation
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drug effects
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Lymphocytes
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cytology
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drug effects
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Male
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Rats
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Rats, Sprague-Dawley
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Selenium
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pharmacology
9.Primary T-cell lymphoma of thyroid: report of a case.
Lu-ying ZHENG ; Qiang LIU ; Yi-ming CHEN
Chinese Journal of Pathology 2009;38(11):774-774
Aged
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CD3 Complex
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metabolism
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Humans
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Immunohistochemistry
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Leukocyte Common Antigens
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metabolism
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Lymphoma, T-Cell, Peripheral
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drug therapy
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metabolism
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pathology
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surgery
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Male
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Neoplasm Invasiveness
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Neoplasm Recurrence, Local
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Thyroid Neoplasms
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drug therapy
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metabolism
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pathology
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surgery
10.Functional and morphological outcome after pyeloplasty for children with unilateral hydronephrosis
Maoxian LI ; Yi YANG ; Ying HOU ; Hui CHEN ; Zhibin NIU
Chinese Journal of Urology 2016;37(6):431-435
Objective To analyze the factors associated with the outcome of pyeloplasty in term of renal function and morphology improvement in children with unilateral ureteropelvic junction obstruction,in order to provide clinical evidence for the treatment of hydronephrosis in children.Methods Clinical data of 174 children who underwent unilateral dismembered pyeloplasty from January 2009 to June 2014 were retrospectively studied.Differential changes in renal function and renal morphology after pyeloplasty were assessed by serial renal scan and ultrasound.On the basis of preoperative split DRF,these patients were divided into three groups:group Ⅰ with DRF ≥40% (n =99),group Ⅱ with DRF 30%-40% (n =29) and group Ⅲwith DRF < 30% (n =46).According to their age at surgery,the children were divided into four groups,including group A aged 1-3 months (n =52),group B aged 3 months-3 years (n =44),groupCaged3-6years (n =37),and group D aged more than 6 years (n =41).Results Inall 174 children,postoperative complication occurred in 7 cases,including urinary tract infection in 6 cases and renal atrophy in 1 case.A significant improvement of both function and morphology was confirmed in most patients (P < 0.01).Patients in group Ⅰ showed stable renal function after operation(DRF 48.46% ±4.80% vs.50.78% ± 5.45%,P < 0.01),of them who underwent pyeloplasty at 1-3 months of age showed the best obvious recovery of renal morphology.Renal function of patients in group Ⅱ recovered obviously and most of them reached to the initial values (DRF 35.18% ± 2.95% vs.43.91% ± 6.89%,P < 0.01).While renal function of patients in group Ⅲ recovered significantly after surgery,most of them failed to restore the initial values(DRF 20.70% ± 6.90% vs.33.78% ± 12.49%,P < 0.01),and among them,the aged 1-3 months group possessed the best recovery.Moreover,the morphological improvement was similar to the functional improvement.The time for hydronephrosis recovered to less than Grade 2 of Society for Fetal Urology(SFU)was 6,24 and over 24 months respectively in group Ⅰ,Ⅱ and Ⅲll,and the renal morphology gradually improved with the increasing duration of follow-up.Conclusions The renal function and morphology of most patients improved significantly after pyeloplasty.Recovery of renal function and morphology after surgery was significantly correlated with the preoperative DRF.Early surgical intervention may improve the function and morphology recovery of the involved renal unit.