1.Pancreatic ?-cell dysfunction and type 2 diabetes
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Both ?-cell dysfunction and insulin resistance contribute to the development of type 2 diabetes, and ?-cell function, especially early insulin secretion, is important in maintaining normal glucose tolerance. This review focuses on the ciritical role of ?-cell dysfunction in the pathogenesis of type 2 diabetes.
2.Changes of T-lymphocyte Subsets Associated with Metabolic Syndrome in the Aged
Xiaoyan WU ; Yi LU ; Xiaomei SHI
Chinese Journal of Rehabilitation Theory and Practice 2008;14(5):424-426
Objective To investigate the correlation of subtypes of the lymphocytes with metabolic syndrome in the aged.Methods 955 aged male and 150 aged female were recruited to a cross-sectional case-control study.CD3+,CD4,CD8+,CD19+,NK cell was measured with flow cytometry.ResultsCD3+,CD19+,NK cells showed no difference in cases and in controls.CD4+/CD8+ ratio declined in subjects with metabolic syndrome,and the ratio declined further with the more criteria.The analysis of multiple analysis regression showed that metabolic syndrome depressed CD4+/CD8+ ratio in the aged.Conclusion Metabolic syndrome was correlated with the disorder of immunity.
4.FcγRIIA H/H131 genotype is associated with pneumonia:a meta-analysis
Min WANG ; Xiaodong WU ; Ting WU ; Xin SU ; Yi SHI
Chinese Journal of Infection and Chemotherapy 2015;(5):415-420
Objective To investigate the association between FcγRIIA H/H131 genotype and pneumonia through a comprehensive meta‐analysis .Methods Databases including PubMed ,Embase ,and China National Knowledge Infrastructure (CNKI) were searched with such terms as “FcgammaRIIa” ,“FCGR2A” ,“Fcgamma receptor IIA” ,“CD32” and“pneumonia” ,“polymorphism” ,“variant” ,“genotype” ,“allotype” ,as well as“pneumonia”and“polymorphism”in Chinese to identify relevant studies .Two reviewers independently extracted the data .Crude odds ratios (ORs) and corresponding 95%confidence intervals (CIs) were estimated .Heterogeneity across studies and publication bias were evaluated and sensitivity analyses were performed .Results Six case‐control studies were finally included in this analysis .The results indicated an association between FcγRIIA H/H131 genotype and pneumonia susceptibility (OR 1 .67 , 95% CI 1 .11‐2 .51 ) , but no significant association with R/R genotype(OR 1 .09 ,95% CI 0 .85‐1 .40) or H/R genotype(OR 0 .81 ,95% CI 0 .60‐1 .19) . Statistically significant heterogeneity was found during data pooling (I2 was 75 .4 % ,51 .8% and 71 .9% ) .Sensitivity analyses further indicated the reliability of the study .Egger′s and Begg′s tests identified publication bias in R/R genotype ,but not in H/H or H/R genotype .Conclusions We preliminarily find an association between FcγRIIA H/H131 genotype and pneumonia susceptibility .
5.Analysis of risk factors for stroke in elderly patients
Hangli LUO ; Hongfei HE ; Xin WANG ; Yi WU ; Chanhong SHI
Chinese Journal of Geriatrics 2015;34(2):138-140
Objective To analyze the relative factors for the prognosis in elderly patients with stroke,and provide theoretical basis for improving prognosis.Methods 188 elderly patients with stroke were treated in our department from Jan.2011 to Nov.2013,and clinical data was analyzed.The prognosis and relative risk factors for stroke were statistically analyzed.Results 99 cases had good prognosis,89 cases had poor prognosis,and the recovery rate were 52.7% (99/188) and 47.3% (89/188) respectively.The age was older in group of good prognosis than in group of poor proghosis (t=1.7930,P<0.05).Univariate analysis showed that the age,position of cerebral infarction,size of lesions,hypertension,diabetes,drinking and smoking were associated with the prognosis of stroke (t=1.7930,x2 =8.6473,15.046,13.1334,4.1536,5.7004,5.9043,respectively,all P<0.05),while the prognosis was not associated with gender,a family history of stroke,hyperlipidemia and heart disease.Multivariate logistic regression analyses revealed the prognosis of stroke was related with risk factors of age (OR=1.072),size of the lesion (OR=3.481),hypertension (OR=2.034),diabetes (OR=1.631) and smoking (OR=3.144).Conclusions It is beneficial to the prognosis of patients with stroke to stop bad habits and to treat hypertension and diabetes actively.
6.Aspergillus fumigatus conidia inhibit early apoptosis of alveolar epithelial cells
He SUN ; Ting WU ; Xiaoyong XU ; Xin SU ; Yi SHI
Chinese Journal of Infectious Diseases 2015;(7):420-423
Objective To observe the impact of Aspergillus fumigatus conidia on the early apoptosis of alveolar epithelial cells.Methods A549 cells was first stimulated with tumor necrosis factor-α(TNF-α)or staurosporine (STS)to induce cell apoptosis,and then co-cultured with Aspergillus fumigatus conidia of three different concentrations (5 × 10 5 ,5 × 10 6 ,5 × 10 7 )simultaneously.The changes of the apoptosis rate of A549 cells were detected by flow cytometry.Results The early apoptosis of A549 cells was increased markedly after stimulated with TNF-α ([14.94 ± 0.82 ]% vs [3.26 ± 0.74]%,χ2 =9.18,P <0.05)and STS ([18.10±1 .00]% vs [3.26±0.74]%,χ2 =12.32,P <0.05). When Aspergillus fumigatus conidia of different concentrations were added,the apoptosis rate was decreased (χ2 =31 .23,P <0.05 ).The early apoptosis of A549 cells was negatively correlated with the concentration of Aspergillus fumigatus conidia (rTNF = - 0.92,r STS = - 0.94,both P < 0.05 ). Conclusion Aspergillus fumigatus conidia inhibits the early apoptosis of A549 cells induced by TNF-αor STS and the inhibit effect increases with the concentration of Aspergillus fumigatus conidia.
7.Clinical study of recombinant human tumor necrosis factor receptor Ⅱ :IgG Fc protein for injection and methotrexate in the treatment of rheumatoid arthritis
Chaoqing WU ; Zhanjie WEI ; Aiping SHI ; Yi WANG
Chinese Journal of Postgraduates of Medicine 2011;34(26):6-8
ObjectiveTo evaluate and compare the efficacy and adverse effects of recombinant human tumor necrosis factor receptor Ⅱ : IgG Fc protein for injection (etanercept) and methotrexate ( MTX )regimens for rheumatoid arthritis (RA). MethodsForty-six patients were randomly divided into two groups by stratified sampling method:etanercept group,22 patients were treated with 12.5 mg of etanercept,twice times per week by subcutaneous injection;and MTX group,24 patients were treated with 5-10 mg of MTX,once per week by oral administration. The course of treatment lasted 24 weeks in both groups, so as to observe their ACR20, ACR50, ACR70 and adverse effects of the drugs. ResultsAfter treated for 24 weeks,ACR50,ACR70 respectively achieved 54.5% (12/22),31.8% (7/22) in etanercept group,which were significantly higher than those in MTX group[16.7%(4/24),4.2%( 1/24)]. The total efficacy was 86.4% (19/22) in etanercept group, 20.8% (5/24) in MTX group, the difference between the two groups was significant (P < 0.05 ). The incidence of adverse effects was not significant between the two groups [22.7%(5/22) vs. 50.0% (12/24)](P > 0.05). ConclusionEtanercept has a good efficacy and safety for the treatment of active RA.
8.Research on Influence of Cervus and Cucumis Polypeptide on the Bone Biomechanical Properties of Ovariectomized Rats
Hua CHEN ; Xiaoyan YI ; Lianguo WU ; Xiaolin SHI ; Mixiong YANG
Journal of Zhejiang Chinese Medical University 2013;(8):1015-1017,1022
[Objective]To study whether Cervus and Cucumis Polypeptide(CCP ,Lugua polypeptide) can treat osteoporosis or not. [Methods]Forty 4-month-old female SD rats were randomized into four groups(10 in each group) ,including SHAM,0VX, OVX+CCP1 and OVX+CCP2 groups. Male rats in OVX groups take the ovariectomized surgery. Since the 13th week, rats in groups A and B got 0.9% physiological saline injection once in a day, group C was treated with CCP 0.4mL/(kg·d)injection and group D treated with CCP0.8mL/(kg·d) injection once one day. Twelve weeks later, al rats were sacrificed for obtaining specimen. Took right femurs, measured the bone biomechanical properties. [Results ]After 12 weeks since injection, the biomechanical property of right femurs in group OVX was significantly lower than that of the SHAM group( P<0.01); parameters such as maximum load and elastic load in group OVX+CCP1 and OVX+CCP2 were higher than that of group OVX(P<0.05), but lower than that of group SHAM(P<0.05). The distinction of the above parameters was significant( P<0.05)between group OVX+CCP1 and OVX+CCP2. [Conclusion]1.CCP can significantly im-prove the bone internal biomechanical properties in ovariectomized(OVX) rats, so it has function to treat osteoporosis caused by drop in estrogen level. There are differences of bone biomechanical properties aspects when treated with different dosage of CCP, which shows the tendency of dose-dependent effect. So we can conclude that CCP may have dose-dependent effect.
9.Inhibitory effect of isobavachalcone on migration and invasion of Tca8113 cells and its mechanism
Yi SHI ; Weizhong WU ; An HUO ; Wei ZHOU ; Zhitu ZHU
Chinese Pharmacological Bulletin 2015;(12):1741-1744,1745
Aim To explore the inhibitory effect of isobavachalcone (IBC)on migration and invasion of
tongue squamous cell carcinoma Tca81 1 3 cells and its possible mechanism.Methods Tca81 1 3 cells were
treated in different concentrations of IBC in vitro.Cell proliferation was detected by MTT;Wound healing as-say and Transwell chamber assay were used to detect the ability of cell migration and invasion;Western blot was applied to detect the expression of Akt,p-Akt, MMP-2 and MMP-9 proteins.Results IBC could in-hibit the proliferation of Tca81 1 3 cells in a concentra-tion-and time-dependent manner.IBC can reduce cell migration and invasion.Western blot showed that IBC could an decrease the expression of p-Akt,MMP-2 and MMP-9 proteins in a concentration-dependent manner.
However,the level of Akt was not affected by the con-centration of IBC treatment.Conclusion IBC could inhibit the proliferation, migration and invasion in Tca81 1 3 cells and its mechanism may be associated with the down-regulation of MMP-2 and MMP-9 pro-teins and the inhibition of phosphorylation of upstream Akt.
10.A study on biological activity of co-expression plasmid of human tissue plasminogen activator and vascular endothelial growth factor 165
Zhongjun WU ; Yi ZHU ; De SHI ; Shusen ZHENG
Chinese Journal of General Surgery 1997;0(04):-
Objective To observe the co-expression plasmid of tissue plasminogen activator (tPA) and vascular endothelia growth factor165 (VEGF165) in vascular endothelial cell (VEC) and to study the effect of the product on the proliferation of VEC and fibrinolysis activity. Methods pBudCE4.1/tPA-VEGF165 was transfected into VECs by using lipofection. The expression of tPA and VEGF165 at mRNA level was detected by RT-PCR and expression at protein level was detected by Western blot. The fibrinolysis activity of VEC culture solution of transfecting tPA and VEGF165 genes were detected by fibrin plate technique. The VEC and VSMC were cultured with VEC culture solution of transforming tPA and VEGF165 genes, the proliferation of VEC and VSMC were evaluated with 3?H-TdR incorporation and flow cytometry (FCM). Results The expression of tPA and VEGF165 in the transfected VECs was detected. The fibrinolysis activity of transfected VEC culture solution was also detected. tPA and VEGF165 products in VECs elevated proliferation of VEC, while there was no effect on the proliferation of VSMC. Conclusion The tPA and VEGF165 eukaryotic co-expression plasmid could express in transfected VECs, and the expression products have biology activity.