2.Effects of ginkgolide (Gin) on cerebral water content, Na+, K(+) -ATPase activity, MDA, lactic acid of rats during acute hypoxia condition.
Jian-Cheng LI ; Shu-Yi JIN ; Xiao-Mei WU
Chinese Journal of Applied Physiology 2003;19(3):239-273
Animals
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Brain
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metabolism
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Female
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Ginkgolides
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pharmacology
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Hypoxia
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metabolism
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Lactic Acid
;
metabolism
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Male
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Malondialdehyde
;
metabolism
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Rats
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Rats, Sprague-Dawley
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Sodium-Potassium-Exchanging ATPase
;
metabolism
3.Application of Nd:YAG laser in stomatology.
Yi DING ; Shimeng XIAO ; Heng YANG ; Shu MENG
West China Journal of Stomatology 2015;33(5):445-450
Developments in science and technology include the use of laser as an auxiliary device in treating oral diseases. Nd:YAG laser is convenient and safe to use. Nd:YAG laser irradiation leaves no scabby area on the wound surface, causes a mild reaction postoperation, and promotes high comfort. Therefore, this treatment has attracted increasing attention in the clinical setting. This review enumerates the applications of water-cooled pulsed Nd:YAG laser in hard and soft tissues in oral medicine. Nd:YAG laser in hard tissues can be applied in cavity preparation, acid etching, root canal preparation and sterilization, and dentin desensitization therapy. Meanwhile, the applications of this laser in soft tissues include adjunctive therapy in basic periodontitis treatment, gingival aesthetic treatment, and resection. This review suggests the importance of Nd:YAG laser as an auxiliary device in the clinical diagnosis and treatment of oral diseases.
Dental Cavity Preparation
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Dentin
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Humans
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Lasers, Solid-State
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Oral Medicine
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instrumentation
;
methods
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Root Canal Preparation
4.Selection and application of biodegradable scaffold in tracheal tissue engineering
Ziyin PAN ; Shu PAN ; Yuanfan XIAO ; Yi ZHONG ; Hongcan SHI
International Journal of Biomedical Engineering 2021;44(1):44-49
Long tracheal lesions are mainly caused by stenosis, infection, trauma, malignant tumors and other factors. Resection of the diseased tissue or stenosis and end-to-end anastomosis is currently the gold standard for long tracheal lesions treatment. However, these treatment programs have proven to have major limitations. In recent years, tissue engineering technology has been regarded as a promising medical alternative treatment method, and the selection of scaffold materials is one of key parts. With the continuous exploration of domestic and foreign researchers, biological materials have been continuously developed and applied to the research of tissue engineering trachea. Tissue engineering degradable scaffold materials can be divided into natural polymer material scaffolds and synthetic polymer scaffolds according to the different sources. The scaffold material can be modified or compounded as needed to improve the biological properties of scaffolds. In addition, with the continuous development of biological printing technology, different scaffold materials can be better combined and used. Biodegradable scaffolds have become a new research direction in the field of tissue engineering trachea due to their polymer properties, and have good application prospects.
5.A novel innate antiviral factor-tetherin.
Yi-Shu YANG ; Xiao-Li WANG ; Ze-Ling LI ; Yi ZENG
Chinese Journal of Virology 2010;26(1):71-75
Animals
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Antigens, CD
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chemistry
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genetics
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metabolism
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Antiviral Agents
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chemistry
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metabolism
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GPI-Linked Proteins
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HIV Infections
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metabolism
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virology
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HIV-1
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genetics
;
physiology
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Human Immunodeficiency Virus Proteins
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genetics
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metabolism
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Humans
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Membrane Glycoproteins
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chemistry
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genetics
;
metabolism
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Protein Binding
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Viral Regulatory and Accessory Proteins
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genetics
;
metabolism
;
Virus Shedding
6.Characteristics of Electrocardiography in Pressure Overload-induced Cardiac Hypertrophy Rats
Yao-Sheng WANG ; Li-Peng HE ; Yi-Hua ZHOU ; Lu-Min XIAO ; Xiao-Shu CHENG ;
Chinese Journal of Hypertension 2006;0(10):-
Objective To analyze the electrocardiography(ECG)data of pressure overload-induced cardiac hy pertrophy rats.Methods Pressure overload cardiac hypertrophy was induced by abdominal aorta constriction. Echocardiogram and heart weight measurement demonstrated the occurrence of cardiac hypertrophy.Standardized ECG parameters of limb and chest were measured and statistically analyzed.Results Two weeks after hypertrophy models were established,echocardiogram showed greater LVPWTd,IVSTd,LVDd.ECG showed that left axis deviation and higher R waves in V_A,V_B,V_C(P
7.Study on PK-PD characteristics of ginsenoside Rg1 and Rb1, in rats with myocardial ischemia following intravenous administration of shengmai injection.
Shu-Yu ZHAN ; Qing SHAO ; Zheng LI ; Yi WANG ; Xiao-Hui FAN
China Journal of Chinese Materia Medica 2014;39(7):1300-1305
It is the objective of this paper to study pharmacokinetics-pharmacodynamics (PK-PD) characteristics of ginsenoside Rg1 and Rb1 on the effect of inducing nitric oxide (NO) release after intravenous administration of Shengmai injection to rats with myocardial ischemia. The model of myocardial ischemia rats was produced by subcutaneous injection of isoproterenol. The serum samples were collected at different time points after intravenous administration of Shengmai injection to rats with the dose of 10.8 mL x kg(-1). The concentrations of ginsenoside Rg1 and Rb1 in serum were determined, and then the concentration-time curves were drawn. Pharmacokinetic parameters of ginsenoside Rg1 and Rb1 were calculated after the construction of pharmacokinetic models. Meanwhile, NO2- and NO3-, the metabolites of NO, in serum were determined, and then the effect-time curve was drawn. The combined PK-PD model was established based on the theory of effect compartment by Sheiner et al. Then pharmacodynamic parameters were calculated. The results indicated that the pharmacokinetics of ginsenoside Rg1 and Rb1 conformed to a two-compartment model. Ginsenoside Rg1 and Rb1 exhibited quick and slow elimination in rats respectively. The effect of Shengmai injection on inducing NO release did not relate directly with and lagged behind the concentrations of ginsenoside Rg1 and Rb1 in serum. The effect exhibited good correlation with ginsenoside Rg1 and Rb1 levels in effect compartment. The relationship between effect and serum concentration fits Sigmoid-E(max) model. This study successfully established the combined PK-PD model of ginsenoside Rg1 and Rb1 after intravenous administration of Shengmai injection to rats. The model can efficiently predict the concentration and effect of Shengmai injection in vivo.
Administration, Intravenous
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Animals
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Ginsenosides
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administration & dosage
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pharmacokinetics
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Humans
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Male
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Myocardial Ischemia
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drug therapy
;
metabolism
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Nitric Oxide
;
metabolism
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Rats
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Rats, Sprague-Dawley
8.Blood vessels and nerves surrounding the seminal vesicles: A clinical anatomic study.
Xiu-ping ZHANG ; Zhao-yi LIN ; Shu-xiong ZENG ; Xiao-dan GUO ; Xiang-qun YANG
National Journal of Andrology 2015;21(10):877-880
OBJECTIVETo investigate the precise locations of the blood vessels and nerves surrounding the seminal vesicles (SV) in men and provide some anatomical evidence for SV-related minimally invasive surgery.
METHODSWe observed the courses and distribution of the blood vessels and nerves surrounding SVs and obtained the data for positioning the SV neuroplexes in 20 male pelvises.
RESULTSOne branch of the neuroplexes was distributed to the SVs bilaterally with the neurovascular bundles, (2.85 ± 0.18) cm from the median sulcus of the prostate (MSP), while another branch ran through the Denonvillier fascia behind the SV, (0.81 ± 0.06) cm from the MSP. The arterial SVs (ASV) originated from the inferior vesical artery and fell into 4 types, 55% going directly to the SVs as one branch, 15% running between the SV and the ampulla of the deferent duct as another branch, 25% downward as 2 branches to the SV and between the SV and the ampulla of the deferent duct respectively, and 5% as the other ASVs. The shortest distance from the ASV through the prostatic neuroplexus to the posterior SV was (1.08 ± 0.09) cm.
CONCLUSIONIn SV resection, neuroplexus injury can be reduced with a bilateral distance of < 2.85 cm and a posterior distance of < 0.81 cm from the MSP, and so can bleeding by vascular ligation between the SV and the ampulla of the deferent duct.
Biopsy ; Humans ; Male ; Prostate ; blood supply ; innervation ; Seminal Vesicles ; blood supply ; innervation ; Vas Deferens ; blood supply ; innervation
9.The in vitro anti-atherosclerotic activity of compound E0869.
Xiao WANG ; Chang LIU ; Peng LIU ; Ni LI ; Yan-Ni XU ; Shu-Yi SI
Acta Pharmaceutica Sinica 2015;50(4):440-446
ATP-binding cassette transporter A1 (ABCA1) and scavenger receptor class B type I (SR-BI/CLA-1) are the key proteins in reverse cholesterol transport (RCT). The high expression of ABCA1 and SR-BI/CLA-1 can decrease the danger of atherosclerosis. The purpose of the study is to find ABCA1 and CLA-1up-regulators for treating atherosclerosis by using cell-based high throughput screening models. Among 20 000 compounds screened, E0869 [1-(3, 4-dimethylphenyl)-1-oxopropan-2-yll4-((methylsulfonyl)methyl)benzoate] was found as the positive hit. The up-regulated activities of E0869 in ABCAl1-LUC and bCA-l1-LUC HepG2 cell were 160% and 175%, respectively. The EC50 values of E0869 in ABCAl1-LUC and CLA-l1-LUC HepG2 cell were 3.79 and 1.42 pμol- x ,(-1) respectively. E0869 could upregulate the mRNA and protein levels of ABCA1, SR-BI/CLA-1 and ABCGJ1genes in HepG2 and RAW264.7 cells by Real-Time Quantitative PCR and Western blotting analysis, but could not influence the expression of FAS, SREBP-l1 and CD36. Foam cell assay showed that E0869 could inhibit lipids accumulation in mouse peritoneal macrophages RAW264.7. Cholesterol efflux assay showed that E0869 could induce HDL-mediated cholesterol efflux in mouse peritoneal macrophages RAW264.7. In conclusion, E0869 could up-regulate ABCA1 and CLA-1 activity, and had good anti-atherosclerotic activity in vitro.
ATP Binding Cassette Transporter 1
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metabolism
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Animals
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Atherosclerosis
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drug therapy
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Biological Transport
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Cholesterol
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Hep G2 Cells
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High-Throughput Screening Assays
;
Humans
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Macrophages, Peritoneal
;
drug effects
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Mice
;
RNA, Messenger
;
Scavenger Receptors, Class B
;
metabolism
;
Up-Regulation
10.Using multivariate multilevel logistic model to study the influential factors of attitudes related to the Punishment on Smoking, among the residents in Guangzhou
Shu-Dong ZHOU ; Yan-Hui GAO ; Xiao-Hua YE ; Yi YANG
Chinese Journal of Epidemiology 2012;33(4):378-381
Objective To explore the attitudes towards the Smoking Ban policy and the bias in different levels of related society sanctions so as to provide evidence for implementing anti-smoking measures in the public places.Methods A multi-univariate-multilevel logistic model was developed to find the relation between penalty attitudes and the covariates' effects.Results 74.6% (3647/4892) and 61.2% (2994/4892) of the residents agreed to punish either the managers of public places or to the smokers.The two kinds of attitude were associated (x2 =1253.45,P<0.0001 ).Residents being female,aged between thirty and fifty-nine years old,having had higher than college education,being non-current smokers,with high scores on knowledge of tobacco control,tended to have the attitude of punishing the public place managers and smokers.However,those persons exposed to everyday secondhand smoke or being non-current smokers,tended to punish the smokers in the generalized Wald test.Conclusion There was strong evidence noticed that most of the residents in Guangzhou had supported the ‘tobacco control penalty'.Factors as age,sex,level of education received,knowledge on hazards of smoking,being current smokers and under secondhand smoke exposure were the influencing factors.Non-current smokers and those who exposed to secondhand smoke everyday,would prefer to take different penalties policies.