1.Reversal effect of single intravenous injection of erythropoietin on vasospasm in the basilar artery following subarachnoid hemorrhage
Hong YIN ; Rui XIA ; Yi-Sa SHI ; Yong-Feng MA ; Uing-Liang YI ; Jin LIU
Chinese Journal of Neuromedicine 2010;09(7):682-685
Objective To investigate the reversal effect of intravenous injection of erythropoietin on vasospasm in the basilar artery in rabbits subjected to subarachnoid hemorrhage (SAH). Methods Thirty male New Zealand adult white rabbits weighing 2.0-2.5 kg were equally randomized into 5 groups: sham-operated group, SAH0 group, SAH1 group, SAH2 group and SAH3 group. SAH models of single-hemorrhage were established by injecting autologous arterial blood (1.0 mL/kg) into the cistema magna in SAH group's rabbits, while animals in the sham-operated group received an injection of normal saline (1.0 mlAg). Twenty-four h after SAH, rabbits in the sham-operated group and the SAHO group were received intravenous injection of normal saline (0.1 mL/kg), meanwhile, intravenous injection of r-Hu-EPO at dosages of 500,1000, or 2000 IU/kg was initiated in the SAH1 group, SAH2 group SAH3 group, respectively. All animals were sacrificed by perfusion-fixation 48 h after SAH induction. The cross section areas of basilar arteries, corrugation coefficient (CC) of the basilar arteries, and hippocampus normal neuron density of CA1 area were measured by Image-Pro-Plus software. Results Morphology observation on the basilar arteries showed no obvious changes in the sham-operated group, significantly thickened and disorganized vascular walls with thickened tunica media and disorganized smooth muscle in the SAH0 and SAH1 groups; the internal elastic lamina in the SAH3 group was folded. Compared with that in SAH0 group [(0.07±0.02) mm2], the cross-sectional area of basilar arteries in SAH2 [(0.10±0.01) mm2] and SAH3 [(0.16±0.02) mm2] groups were significantly increased (P<0.05); the CC in SAH2 (1.22± 0.06) and SAH3 (1.15±0.03) groups was significantly lower than that in the SAHO group (1.31±0.09, P<0.05); and the normal neural density of hippocampus in CA1 area in the SAH3 group [(126.8±5.7) cell/mm] was significantly higher than that in the SAHO group [(99.3±9.6) cell/mm, P<0.05]. Conclusion A single intravenous injection of erythropoietin 24 h after SAH can still reverse SAH-induced vasospasm and attenuate injury of neurons.
2.Prevalence and incidence of anemia among school students aged 6-11 years old in Minhang District of Shanghai
dan Dan HE ; sa Xiao WEN ; yi Xin ZHANG ; di Di CHEN ; mei Hong TANG ; li Xiao XU ; Hong FANG ; jing Hui SHI
Fudan University Journal of Medical Sciences 2017;44(5):633-638
Objective To analyze the epidemic features of anemia among students aged 6-11 years old in Minghang District,to provide basis for the control and prevention strategies of anemia among school students.Methods Surveillance on hemoglobin concentration was conducted among 42 872 students aged from 6 to 11 years old between 2012 and 2015.All data analyses were completed by SPSS 18.0 statistical software.Results The prevalence rate of anemia among school students 6-11 years old was 5.05% (95%CI:4.84-5.26).The prevalence rate of anemia among girls was 5.28%,which was significantly higher than that 4.84% among boys (x2 =4.24,P =0.037).Six-year-old boys and girls were most susceptible to anemia.Compared to students in Hope Schools,public school students are more vulnerable to anemia (boys:OR =2.37,95 % CI:2.03-2.76;girls:OR =2.08,95 % CI:1.74 -2.49).Overweight and obese students had a lower risk of anemia than average students (boys:OR =0.65,95 % CI:0.55-0.76;girls:OR =0.75,95 % CI:0.61-0.92).The three-year cumulative incidence of anemia was 12.80% (95 %CI:12.49-13.12).The cumulative incidence among girls was 14.52%,which was significantly higher than that of 11.28% among boys (x2 =100.26,P<0.001).Six-year-old boys have the highest three-year cumulative risk among all students,while 10-11-year-old girls have the highest three-year cumulative incidence.The risk of anemia in Hope School was found highest in all schools (boys:RR =1.93,95%CI:1.72-2.16;girls:RR =1.20,95 %CI:1.04-1.39).Overweight and obesity were considered protective factors to anemia (boys:RR =0.75,95%CI:0.67-0.84;girls:RR =0.77,95%CI:0.68-0.88).The primary and recurrent detection rates of anemia were 14.58% and 2.54%.The anemia among students was mainly detected during the first examination despite the differences in ages,schools and nutrition conditions (P < 0.05).Conclusions The prevalence of anemia among primary school students in Minhang District is at a mild level.Control measures should be adopted,including monitoring of hemoglobin in all school children and adolescents,taking individual intervention measures based on results,promoting the health education for adolescent girls and parents of school children,and paying special attention to Hope School and students with recurrent and continuous anemia.
3.Association between calcium-sensing receptor protein expression and rat cardiomyocyte apoptosis.
Yi-Hua SUN ; Chang-qing XU ; Hong LI ; Sa SHI ; Wei-hua ZHANG ; Ya-jun ZHAO ; Yan-qiao ZHANG ; Wei-min HAN ; Li-ping HAN ; Chun-ming JIANG ; Quan-feng LI ; Rui WANG
Chinese Journal of Cardiology 2006;34(8):739-743
OBJECTIVETo investigate the relationship between calcium-sensing receptor protein (CaSR) expression and rat cardiomyocyte apoptosis and related signal transduction pathways.
METHODSThe CaSR, BCl2, Caspase3 protein and ERK1/2 phosphorylation or non-phosphorylation were detected by Western blot. Cardiomyocyte apoptosis was detected by flow cytometry and immunofluorescence.
RESULTSCaSR protein was detected in rat cardiac tissue and CaSR activator gadolinium (GdCl3) induced cardiomyocyte apoptosis and increased ERK1/2 phosphorylation and expression of BCl2 and activated Caspase3. The selective mitogen-activated protein kinase (MAPK) inhibitor PD98059 abolished gadolinium -induced ERK1/2 activation and BCl2 expression, further increased the activation of Caspase3 and cardiomyocyte apoptosis.
CONCLUSIONOur results demonstrate the CaSR existence in cardiomyocytes and CaSR activation by gadolinium can induce myocyte apoptosis by activating Caspase3 and tyrosine protein kinase pathway.
Animals ; Apoptosis ; Caspase 3 ; metabolism ; Female ; Male ; Myocardium ; metabolism ; Myocytes, Cardiac ; metabolism ; RNA, Messenger ; biosynthesis ; Rats ; Rats, Wistar ; Receptors, Calcium-Sensing ; biosynthesis ; genetics ; Signal Transduction
4.Bioinformatics analysis of programmed cell death ligand 1 co-expression genes and their regulatory network in head and neck squamous cell carcinoma.
Li-Sa YANG ; Cong-Yu SHI ; Yu-Hao LIANG ; Tong LIU ; Xiao-Ru HOU ; Xu-Dong TIAN ; Xiao-Yi WANG
West China Journal of Stomatology 2019;37(5):516-520
OBJECTIVE:
This study aimed to construct a network of programmed celldeath ligand 1 (PD-L1) co-expression genes and screen potential biomarkers for PD-L1 expression in head and neck squamous cell carcinoma (HNSCC) by bioinformatics analysis. Moreover, the genes and pathways participating in PD-L1 and regulating the tumor immune status were determined.
METHODS:
The HNSCC transcriptomic dataset in TCGA was selected to retrieve gene sets on the cBioPortal platform, where PD-L1 co-expressional genes were acquired. With these genes, GO-BP, KEGG, and string analyses were performed in R clusterProfiler. Cytoscape was used for network analysis and hub gene screening.
RESULTS:
A total of 117 co-expression genes were obtained, most of which were enriched in immune regulation and response to viral processes. Node degree analysis indicated that STAT1, IFNG, CXCL10, CCR5, FCGR3A, CXCL9, GBP5, CD86, GZMB, IRF1 were the highest connected genes and functioned as hub genes. Survival analysis of these hub genes resulted in CCR5, CXCL9, and GZMB as the prognostic biomarkers for patients with HNSCC, all of which were involved in immune regulation and their expression levels were related to PD-L1 (Pearson correlation coefficient was 0.30, 0.35, 0.39; P<0.01). High expression levels of these three hub genes were protective factors in patients with HNSCC.
CONCLUSIONS
PD-L1 co-expression hub genes are related to immunity, among which CCR5, CXCL9, and GZMB are prognostic markers with the possibility to be involved in programmed cell death protein 1 (PD-1)/PD-L1-induced tumor immune escape. These genes provide new clues to study the mechanism and precision target medicine of PD-1/PD-L1 in HNSCC.
B7-H1 Antigen
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Carcinoma, Squamous Cell
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Computational Biology
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Head and Neck Neoplasms
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Humans
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Receptors, IgG
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Squamous Cell Carcinoma of Head and Neck