1.Expression of E-cadherin and beta-catenin in Bowen's disease and squamous cell carcinoma.
Zheng-xiao LI ; Zhen-hui PENG ; Fan-pu JI ; Jing-yi YUAN ; Min PAN ; Yan LIU ; Qiong WANG
Journal of Southern Medical University 2006;26(8):1245-1247
OBJECTIVETo investigate the involvement of E-cadherin-catenin adhesion system in Bowen's disease (BD) and cutaneous squamous cell carcinoma (SCC).
METHODSFifteen normal skin, 28 BD and 18 SCC specimens were stained with monoclonal antibodies against E-cadherin and beta-catenin. Evaluation of the staining results was performed with semi-quantification of the pattern and intensity of staining, percentage of positive cells, and cytoplasmic staining.
RESULTNormal skins strongly expressed membranous E-cadherin and beta-catenin, but their expression was remarkably reduced in BD and SCC. Abnormal staining of beta-catenin was observed in the cytoplasm or cell nuclei of BD and SCC.
CONCLUSIONAbnormal expression of the E-cadherin/catenin complex is common in SCC and BD.
Adult ; Aged ; Bowen's Disease ; metabolism ; pathology ; Cadherins ; biosynthesis ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Skin Neoplasms ; metabolism ; pathology ; beta Catenin ; biosynthesis
2.Advances in extraction and analysis of phenolic compounds from plant materials.
Cong-Cong XU ; Bing WANG ; Yi-Qiong PU ; Jian-Sheng TAO ; Tong ZHANG
Chinese Journal of Natural Medicines (English Ed.) 2017;15(10):721-731
Phenolic compounds, the most abundant secondary metabolites in plants, have received more and more attention in recent years because of their distinct bioactivities. This review summarizes different types of phenolic compounds and their extraction and analytical methods used in the recent reports, involving 59 phenolic compounds from 52 kinds of plants. The extraction methods include solid-liquid extraction, ultrasound-assisted extractions, microwave-assisted extractions, supercritical fluid extraction, and other methods. The analysis methods include spectrophotometry, gas chromatography, liquid chromatography, thin-layer chromatography, capillary electrophoresis, and near-infrared spectroscopy. After illustrating the specific conditions of the analytical methods, the advantages and disadvantages of each method are also summarized, pointing out their respective suitability. This review provides valuable reference for identification and/or quantification of phenolic compounds from natural products.
Phenols
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chemistry
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isolation & purification
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Plant Extracts
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chemistry
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isolation & purification
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Plants
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chemistry
3.Progress in active compounds effective on ulcerative colitis from Chinese medicines.
Si-Yu CAO ; Sheng-Jie YE ; Wei-Wei WANG ; Bing WANG ; Tong ZHANG ; Yi-Qiong PU
Chinese Journal of Natural Medicines (English Ed.) 2019;17(2):81-102
Ulcerative colitis (UC), a chronic inflammatory disease affecting the colon, has a rising incidence worldwide. The known pathogenesis is multifactorial and involves genetic predisposition, epithelial barrier defects, dysregulated immune responses, and environmental factors. Nowadays, the drugs for UC include 5-aminosalicylic acid, steroids, and immunosuppressants. Long-term use of these drugs, however, may cause several side effects, such as hepatic and renal toxicity, drug resistance and allergic reactions. Moreover, the use of traditional Chinese medicine (TCM) in the treatment of UC shows significantly positive effects, low recurrence rate, few side effects and other obvious advantages. This paper summarizes several kinds of active compounds used in the experimental research of anti-UC effects extracted from TCM, mainly including flavonoids, acids, terpenoids, phenols, alkaloids, quinones, and bile acids from some animal medicines. It is found that the anti-UC activities are mainly focused on targeting inflammation or oxidative stress, which is associated with increasing the levels of anti-inflammatory cytokine (IL-4, IL-10, SOD), suppressing the levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8, IL-23, NF-κB, NO), reducing the activity of MPO, MDA, IFN-γ, and iNOS. This review may offer valuable reference for UC-related studies on the compounds from natural medicines.
Animals
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Colitis, Ulcerative
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drug therapy
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pathology
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Cytokines
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metabolism
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Disease Models, Animal
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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therapeutic use
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Humans
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Inflammation
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drug therapy
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metabolism
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Medicine, Chinese Traditional
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Oxidative Stress
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drug effects
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Phytochemicals
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pharmacology