Objective To investigate the clinical significance of vascular endothelial growth factor (VEGF) levels in the serum of patients with acute myocardial infarction (AMI),and also to explore the relationship between VEGF and creatine kinase (CK) levels in AMI.Methods 25 patients with AMI and 12 controls were used for this study. Serum was isolated from peripheral blood on day 1, 5, 10 and15 after the onset of AMI. VEGF levels were measured by enzyme-linked immunosorbant assay (ELISA).Results Serum VEGF levels elevated gradually after the onset of AMI and reached a peak on day 10, which was significantly higher than that of control group (178 9?48 9)pg/ml vs(64 9?16 7)pg/ml,P

Objective To observe the dynamic change of the intimal proliferation in balloon-injured rabbit carotid arteries to explore the mechanism of restenosis. Methods 40 healthy male New Zealand white rabbits were randomly divided into experiment groupsⅠ、Ⅱ、Ⅲ and control group, each group containing 10 rabbits. Right carotid artery was injured with PTCA balloon in the experiment groups. 10 rabbits in each experiment group were killed on 1, 2 and 4 weeks after injury. Morphological change of injured intimae was examined by light microscope and analyzed using a computerized imaging analysis system. Results In experiment group, neointimal areas were significantly more than those in control group(P

Objective:To explore the method of the establishment of edentulous mandible All-on-4 implant photoelastic model with various distally tilted implants by computer-aided design(CAD).Methods:The edentulous mandible specimen was scanned by CT. Three-dimensional models of the mandible consisting of implant location were reconstructed and edited with Mimics and Geomagic Stu-dio software.Each of the four models had four implant sockets in the interforaminal area.In the first model,the bilateral distal im-plants were placed vertically.In the other models,the posterior implants were respectively inclined by 1 5°,30°and 45°distally.The four models were manufactured by RP and then the All-on-4 photoelastic models were made by traditional ways.Results:The ob-tained ethoxyline resin models featured bright surface,homogeneous structure,faintly yellowish,high optical sensibility,precision im-plants location and with no natural stress.The model offered a good experimental basis for the stress measurement.Conclusion:The method of fabricating ethoxyline resin models by CAD is reproducible,which simplifies the operating process.

Objective To explore influence of JAK/STAT3 signaling pathway on the apoptosis of HCC cells.Methods DNA-vector-based RNAi approach silence was used to down-regulate STAT3 expression in Bel-7402 cells.According to the STAT3 cDNA sequence in the GeneBank database,the plasmid pGCsi.U6/neoRFP-STAT3 that was designed for expression of STAT3 siRNA was constructed and synthesized,and then transfected into the Bel-7402 cells with iipofectamine 2000.The apoptotic rate was measured with flow cytometry (FCM) and annexinV/PI apoptosis detection kit staining.The mitochondrial membrane potential (BΨm) was visualized by the JC-1 fluorescence staining and the inverted fluorescence microscope.Moreover,the expression of caspase-3 protein was analyzed by Western blotting.Results The apoptotic ratio of STAT3-siRNA group was (38.82 ± 0.88) ％,which was significantly higher than that in other group [control group (9.22 ± 0.38) ％,scramble-siRNA group (16.47 ± 1.04) ％,P ＜ 0.05].The mitochondrial membrane potential of STAT3-siRNA group observed by the JC-1 fluorescence staining was decreased significantly[(91.33 ± 1.78) ％] and [(89.90 ± 1.92) ％ vs (59.06 ± 1.89) ％,P ＜ 0.05].The Western blot results showed that the protein expression of active caspase-3 in STAT3-siRNA group was significantly higher than other groups (0.48 ± 0.05 vs 0.22 ± 0.04 and 0.26 ± 0.06,P ＜ 0.05).Conclusions STAT3 gene silencing significantly improves the apoptotic effect in the Bel-7402 ceils.

OBJECTIVE: To investigate the allergens in patients with allergic rhinitis in Yichang, so that to find common allergens of Yichang and to provide statistic basis for a reasonable prevention and treatment to allergic rhinitis. METHOD: 1,979 patients with allergic rhinitis in Yichang were detected for allergens by skin prick test and the distribution of positive rates to inhaled allergens was compared between different genders and ages. RESULT: 1,545 (78.1%) of 1,979 suspected allergic rhinitis patients presented positive reaction. The positive rate in male was significantly higher than in female, and that in juvenile group was significantly higher than in adults. Among positive cases in inhalation group, the most common allergen was flour mite (80.4%), followed by house dust mite (64.9%), cockroach (13.3%) and artemisia pollen (8.2%). CONCLUSION The study shows that the flour mite and house dust mite are the most common inhaled allergens causing allergic rhinitis in Yichang. We should pay more attention to the prevention and treatment for the juvenile patients.
Adolescent ; Adult ; Aged ; Allergens ; immunology ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Rhinitis, Allergic ; diagnosis ; epidemiology ; immunology ; Skin Tests ; Young Adult

Objective:To establish a method for isolating adipose derived stem cells(ADSCs)from resected human subcutaneous adipose tissues.Methods:ADSCs were isolated,cultured,and expanded from human subcutaneous adipose tissues.Immuno-fluorescent staining of specific molecules.FACS and multi-lineage differentiation induction were used to characterize the obtained ADSCs.Results:ADSCs obtained in this study had the characteristics of stem cells and expressed specific molecules;they also possessed a multi-lineage differentiation potential,which was genetically stable.Conclusion:ADSCs can be isolated from human subcutaneous adipose tissues,which provides a novel and abundant seeding cells for tissue engineering.

Objective To investigate the application and administration of hyperbaric oxygen chambers in Beijing.Methods Forty-eight hospitals with hyperbaric oxygen chambers in Beijing were investigated by field investigation,letter investigation or telephone investigation.The investigation includes grades of hospital,personnel,equipment,therapy of severe cases,time of therapy and treatment plans in questionnaire.Results Hyperbaric oxygen was popular in Beijing,which ensured emergency treatments.But the scale was little relatively and the number of full-time technicians was less,besides,the therapeutic system and plans were diversified,which should be standardized.Conclusions Something must be done to improve the conditions of hyperbaric oxygen medicine in Beijing,such as cultivating personnel,standardizing therapeutic system,standardizing treatment plans,realizing computer control and so on.

Objective: To observe the morphological changes of enteric deep muscular plexuses interstitial cells of Cajal (ICC-DMP) in rats with multiple organ dysfunction syndrome (MODS). Methods: Forty Wistar rats were randomly divided into control group and MODS model group. The enteric ICC-DMP network was observed using c-kit immunohistochemical staining with whole-mount preparation technique and confocal laser scanning microscopy , and the ultraslructural features of ICC-DMP was evaluated using transmission electron microscope. Results: Compared with those in control group, the distributions and densities of intestine ICC-DMP in MODS group were significantly decreased (P < 0. 05) , the ICC-DMP network was disrupted and the ultrastructural features of ICC-DMP were severely damaged. Conclusion: The ICC-DMP network was severely damaged in rats with MODS, and the mechanism of gastrointestinal dysmotility in MODS may be related to the morphological changes of ICC-DMP.

Objective To analyze the disposition characteristic of LGT fingerprints and the related fingerprints for advanced gastric carcinoma patients when the LGT fingerprints changing. Methods SELDI and CM10 protein chip was used to detect the serum protein fingerprints of 81 cased of advanced gastric cancer patients. After 1 year follow-up, all the patients were detected by SELDI again. According to the expression condition of LGT fingerprints, the patients were divided into 4 groups: A group(LGT changing from negative to positive) 25 cases, B group (LGT changing from positive to negative) 15 cases, C group (LGT fingerprints keeping negative) 29 cases and D group (LGT fingerprints keeping positive) 12 cases. The influencing factor should be rejected. And the remaining fingerprints were LGT fingerprints' subtypes and therelated fingerprints. The different proteomic fingerprints were analyzed by Biomarker Wizard 3.1 Software.Results After rejecting the influencing fingerprints, the up-regulation fingerprints in A group were 11473, 11821, 11664, 11409, 11552 and 11947 (M/Z), and no down-regulation fingerprints. In B group, 3264 was upregulation, and 6523, 11509, 11669, 11413, 11351 and 6483 were down-regulation. In C and D group, there was not statistically differential protein fingerprint. Conclusion M/Z up-regulating fingerprints including 11473, 11821, 11664, 11409, 11552 and 11947 can be regarded as the subtype of LGT when it changing from negative to positive; down-regulating including 11509, 11669, 11413 and 11351 can be regarded as the subtype of LGT when it changing from positive to negative; 3264 up-regulating and 6523 and 6483 downregulating can be regarded as the related fingerprints when LGT changing from positive to negative; and when LGT keeping negative or positive in patients with advanced gastric cancer, there was no differential protein fingerprints.

15, the mortality was38.1%(n = 8).There was significantly difference both in mortality (x2 = 4.14 P