Objective To study the expression of RASSF1A gene in gastric cancer tissues and cell lines and to investigate its clinical roles. Methods Immunohistochemistry was employed to detect the expression of RASSF1A gene in 39 cases of human gastric cancer tissues and 18 cases of normal human gastric tissues, and RT-PCR was used to examine the expression of RASSF1A mRNA in 4 gastric cancer cell lines, normal gastric cell lines GES-1 and positive control Hela cells. Correlations between expression of RASSF1A gene and clinicopathological characteristics of gastric cancer were also analyzed. Results All the normal gastric tissues (18 cases) were stained with anti-RASSF1A antibody, and significantly lower expression of RASSF1A was found in the 39 cases of gastric cancer tissues (P

Objective To explore the feasibility of repairing and strengthening the posterior wall of the inguinal canal with laparoscopic hernioplasty using autologous anterior abdominal wall tissues.Methods From January 2001 to September 2007,173 adult patients with inguinal hernia(141 cases of indirect hernia,28 cases of direct hernia,3 cases of femoral hernia,and 1 case of obturator hernia)were treated by laparoscopic hernioplasty with autologous tissues obtained from the median,internal,and lateral umbilical folds,and the U-shape fold.Three trocars were used to perform the laparoscopy by one surgeon,who completed the intracavity suturing using two hands.Results All the 173 operations were completed without converting to open surgery.Among the 152 cases of unilateral hernia,the mean operation time was 25 min(10-40 min),while in the 21 cases of bilateral hernia,the mean operation time was 40 min(20-60 min).Postoperative complications included retroperitoneal hematoma(1 case),scrotal swelling(3),testicular hydrocele(1),subcutaneous hemorrhage at the inguinal area(2),and scrotitis(1).The patients were followed up for 1-84 months(mean 35 months),during which 3 patients had recurrence.Conclusions Laparoscopic hernioplasty with autologous tissues is feasible and safe for adult patients with inquinal hernia with advantages of short hospitalization,minimal surgical trauma,quick recovery,and low rate of recurrence.

Objective To investigate the autonomic modulation and influence during myocardial ischemia based on analysis on heart rate dynamics with heart rate variability(HRV). Methods From 13 free download ECG records in long-term ST-T(LTST)database, 193 ischemia episodes were extracted for analysis, and 5 min intervals before and after each selected isehemia episode were selected as control ones. Using symbolic dynamic analysis method, the indexes during different stages were calculated. The repeated measurement analysis of variance was introduced for statistic test. And the distribution of autonomic reflex mode to isehemia duration was analyzed by linear fitting. Results Symbolic analysis detected an increase in the percentage of non-varlable patterns (0 V%) and a decrease in the percentage of very variable patterns(2 UV%), indicating a more sympathetic predominance compared with the baseline. But the trend of the shift in autonomic balance towards sympathetic predominance reduced with the extension of ischemic duration, suggesting a possible protection for heart. Conclusion Symbolic dynamic analysis is capable of identifying changes in autonomic modulation and seems appropriate for elucidating the neural pathophysiological mechanisms occurring in myocardial ischemic episodes.

This paper introduces the working flow of the installation and the check & acceptance of the medical equipment.The maior working flow includes the following item.(1)the time of accepting contract.(2)Auditing contract.(3)confirming the arrival time of the equipment.(4)preparing fabricating yard for the equipment installation.(5)the actual time of the equipment arriving.(6)unpacking and inspecting.(7)checking accessories of the equipment.(81collecting the manual of the equipment.(9)the procedure information of the importing equipment.(10)checking the eligibility of the equipment.(11)the operation and the training of the maintenance.(12)the measuring and auditing of the equipment.(13)the maintenance of equipment time.The working flow contributes a lot to our equipment checking & acceptance.In this article,we summarize the experience of the checking &acceptance of the medical equipment.

Sepsis is a common complication in critically ill patients. The incidence rate was significantly higher in recent years, and it has been as the main cause of death in critically ill patients. The traditional treatment measures failed to significantly improve mortality. In recent years, the treatment of sepsis by traditional Chinese medicine are getting far more attention, the combination of Chinese traditional and western medicine treatment of sepsis bring out new treatment ideas and thoughts,and has achieved good results. This paper reviews the TCM recognition of sepsis from the etiology and pathogenesis, syndrome differentiation, treatments, signgle traditional Chinese medicine and compound preparations and so on,following a brief analysis of existing problems and solutions.

Objective To analyze and compare the therapeutic drug monitoring (TDM) and regularity of tacrolimus in deceased donor liver transplantation (DDLT) and living-donor partial liver transplantation (LDLT) the therapeutic drug monitoring (TDM) and regularity of tacrolimus.Method The clinical data of 68 patients undergoing liver transplantation from January 2010 to October 2011 were analyzed retrospectively.Thirty cases underwent LDLT as group A,and the remaining 37 underwent DDLT as group B.Result The time to reach therapeutic window was shorter in group A(3.9 ± 0.7 days) than in group B (6.5 ± 1.0 days,P＜0.01).The tacrolimus dosage in group A was significantly less than that in group B during the first 28 days post-transplantation.However,the tacrolimus dosage approached gradually and tended to be consistent after 28 days.On the postoperative day 7,14,21 and 28,the Tacrolimus dosage in group A was 72.37％,79.31％,90.11％ and 95.45％ of that in group B respectively.Concentration-dose ratio in group B was in a steady state (80-90 g/L).Concentration-dose ratio in group A was decreased with time,culminating at 28th day and close to that in group B.Correlation analysis revealed that graft recipient weight ratio(GRWR) had a significantly positive correlation with the tacrolimus dosage on the first 7 days (r =0.557) and a significantly negative correlation with the tacrolimus concentration/dose ratio (r =-0.578).Conclusion The early tacrolimus dosages in patients subject to LDLT were correlated significantly with the GRWR.The early tacrolimus dosages in patients undergoing LDLT were about 70％ of those in patients undergoing LDLT.However,with the regeneration of the liver,they tended to be consistent after 28 days.

Objective To explore the effects of upper limb robot-assisted therapy on motor function and activities of daily living in con-valescent stroke patients. Methods From June to September, 2016, 12 chronic stroke patients at their first-ever stroke were enrolled and ran-domized into experimental group (n=6) and control group (n=6). Both groups received routine rehabilitation. Additional robot-assisted thera-py was provided to the experimental group, and additional repetitive movement training was provided to the control group, 20 minutes a day, five days a week for four weeks. Fugl-Meyer Assessment-Upper Extremities (FMA-UE), modified Ashworth Scale (MAS) and Func-tional Independent Measure (FIM) were used to assess the motor function of the upper limbs and hands, the muscular tension of shoulder and elbow, and activities of daily living (ADL) before and after treatment. Results After treatment, the scores of FMA-UE and FIM were bet-ter in both groups (Z>2.032, P<0.05), and no significant difference was found between two groups (t<0.723, P>0.05), however, the scores were a little bit higher in the experimental group than in the control group. After treatment, for the experimental group, the MAS scores of shoulder abduction/adduction and elbow flexion/extension improved (Z>2.121, P<0.05);for the control group, the MAS scores of shoulder abduction/adduction improved (Z>2.000, P<0.05), but the MAS scores of elbow flexion/extension were not significantly different (Z<1.890,P>0.05). There was no significant difference in the MAS scores of shoulder abduction/adduction and elbow flexion/extension between two group (Z<1.734, P>0.05). The moving trail recorded by the computer, gradually became a regular pattern from the mass, saying the motor control ability became better. Conclusion Upper limb robot-assisted therapy can promote the recovery of the motor function of upper limbs and ADL in convalescent stroke patient, similar to the repetitive movement training.

Genomics ; Medicine, Chinese Traditional

Adult ; Blast Crisis ; Female ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; complications ; diagnosis ; Ulcer ; diagnosis ; etiology

Objective To investigate the fibrosis-associated genes, especially those transforming growth factor-β(TGF-β) associated, expression in the tissue of extensive fibrotic kidney resulting from severe by-dronephrosis. Methods Compared 12 extensive fibrotic kidneys resulting from severe hydronephrosis with 6 normal renal tissues using PCR array. All samples were collected from our hospital from Aug. 2005 to Dec. 2006. The data were treated with △△Ct method. When 2△△Ct≤<0.5 or≥2, the difference was considered sig-nificant. Results A total of 49 differential genes expressed in the fibrotic renal tissues were screened out, of which, 25 were up-regulated and 24 were down-regulated. The differential genes included members and receptors of TGF-β super family and bone morphogenetic protein(BMP) family, target molecule of TGF-β or BMP signal pathway and several regulators. Conclusions Through investigating with PCR array, we certifi-cated the positive effect of Nodal, GSC and IGFI to renal fibrosis. And we suppose that TGF-β play a sec-ondary role in renal fibrosis, while BMP family and Leftyl work in a functional genome in the regulating of renal fibrosis.