1.Comparative study on Argus and artificial methods for MRI scanning of femoral head necrosis area
Yuru DONG ; Hong WANG ; Hu FENG ; Xuetao MU ; Yi MA ; Na LI ; Mian LIU
Chinese Medical Equipment Journal 2015;36(5):70-72,97
Objective To explore the advantages of Argus method by comparing the accuracy and timeliness of Argus and artificial methods for measuring femoral head necrosis area in MRI scanning.Methods Totally 17 patients (31 hips) were measured with Argus and artificial methods respectively for the necrosis area, and then the measuring results and time were compared, and the correlation was investigated between the results and the patients' pain degree, along with that between the results and the extent of femoral head collapse.Results The necrosis area ratios determined by Argus and artificial methods were (33.5±4.08)%and (34.6±4.06)%respectively, with no statistical difference between the ratios (P>0.05). The time consumed by artificial method was (21.3 ±3.62)min, significantly longer than (7.89 ±1.03)min by Argus method, with P<0.001. Regression analysis proved that the necrosis areas were positively correlated with the patients' pain degree, and the correlation coefficient by Argus method was 0.807 8, more than 0.740 9 by artificial method. The femoral heads of 11 cases(16 hips) collapsed in the follow-up period, the necrosis areas were positively correlated with the patients collapse level, but the correlation coefficient by Argus method was 0.783 8, more than 0.726 7 by artificial method.Conclusion Argus method gains high accuracy and timeliness when used in MRI scanning of femoral head necrosis area, and thus is worth popularizing clinically.
2. Cloning and identification of two squalene synthase genes from Salvia miltiorrhiza
Chinese Traditional and Herbal Drugs 2014;45(9):1307-1312
Objective: To clone and identify the squalene synthase genes in Salvia miltiorrhiza. Methods: The primers were designed based on the predication result of S. miltiorrhiza genome genes and two squalene synthase genes (SmSQS1 and SmSQS2) from S. miltiorrhiza were amplified via RT-PCR method. Some bioinformatic methods and softwares were used for the gene structure analysis, the analyses of sequence homology and protein conserved domains of the two gene encoding polypeptides were carried. Real-time quantitative PCR (RT-qPCR) method was used for the analysis of gene expression patterns. Results: The two squalene synthase genes of S. miltiorrhiza were obtained by RT-PCR method. The encoded polypeptide of the two genes has the related domains and a conserved motif for squalene synthases. The two genes had the different exon/intron characteristics and the different tissue-specific and time-specific expression patterns. Conclusion: There are two squalene synthase genes existing in the genome of S. miltiorrhiza, which have the different gene structures and expression patterns, and probably play the different roles in the biosynthesis process of steroids and triterpenoids in S. miltiorrhiza.
3.Transcriptome analysis reveals candidate genes involved in flavonoid biosynthesis in Ziziphora bungeana.
Jiang HE ; Yi-Mian MA ; Wei-Jun YANG ; Bo CHENG ; Li-Nu-Er DI ; Li-Na MA ; Guan LI
China Journal of Chinese Materia Medica 2019;44(15):3178-3186
Ziziphora bungeana is a kind of medicinal plants belongs to Labiatae,and it also a kind of geoherbs in Xinjiang. The main active ingredient linarin has a higher content in inflorescence than in other parts. In this study,high-throughput sequencing technology was used to reveal the transcriptome of the inflorescence of Z. bungeana,77 366 unigenes were acquired,of which 56 375 unigenes were annotated based on search of the database and classification. Through the analysis of metabolic pathways,sixty unigenes were probably encoding some enzymes involved in the flavonoid biosynthesis pathways. The contents of linarin in different parts were determined and the key genes were verified by qRT-PCR. The discovery provides the research basis for further analysis of the enzyme genes involved in the biosynthesis of the major flavonoid components in Z. bungeana.
Flavonoids
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biosynthesis
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Gene Expression Profiling
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High-Throughput Nucleotide Sequencing
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Lamiaceae
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chemistry
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Transcriptome
4.Development and Application of Automatic Analysis and Surveillance Platform for Chrimerism in Donors and Recipients after Allo-HSCT.
Jian-Cheng FANG ; Fang WANG ; Li-Li YUAN ; Mian-Mian WANG ; Ting-Ting LI ; Yi-Hang YANG ; Yang LIU ; Xiao-Li MA ; Xue CHEN ; Yang ZHANG ; Dai-Jing NIE ; Jia-Qi CHEN ; Hong-Xing LIU
Journal of Experimental Hematology 2020;28(3):1012-1018
OBJECTIVE:
To develop an automated chimeric analysis and reporting platform based on short tandem repeat (STR) and capillary electrophoresis methods for allogeneic hematopoietic stem cell transplantation (allo-HSCT) so as to improve work efficiency.
METHODS:
Apache, MySQL, PHP and HTML5 were used to build the database and interface. The STR locus geno typing and chimeric analysis logic and flow were set up on the basis of STR rules and capillary electrophoresis. STR genotyping and 194 times of chimeric testing data of 100 patients after allo-HSCT were used to test the platform for automatic STR locus genotyping, chimeric calculation and report generation.
RESULTS:
The established platform could realize the functions of STR locus customization, STR genotype determination, automatic chimeric analysis, and detection information database management, which can automatically generate an integrated report including multiple sequential chimeric results and trend graphs for the same patient and can be accessed and used simultaneously by different users through different browser interfaces. The results of automated analysis by the platform are completely consistent with that of manual analysis by experienced technicians, and the possibility of manual analysis error is reduced through automation. The time required for automatic analysis using this platform is approximately 1/6-1/5 of manual analysis.
CONCLUSION
The automatic analysis platform built in this study is operation stable and reliable in analysis results, which can improve work efficiency and report connotation, thus worthing popularized and applicable.
Electrophoresis, Capillary
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Genotype
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Hematopoietic Stem Cell Transplantation
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Humans
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Microsatellite Repeats
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Tissue Donors
5.Mechanism concerning antitumor effect of oridonin on multiple myeloma cell line U266.
Hao-Qing DUAN ; Mian-Yang LI ; Li GAO ; Jun-Feng ZHANG ; Wei WANG ; Yan LI ; Yi-Gai MA ; Cheng-Bin WANG ;
Journal of Experimental Hematology 2014;22(2):364-369
This study was purposed to investigate the antitumor effect of oridonin on human multiple myeloma cell line U266 and its possible mechanism. The CCK-8 test was used to determine the inhibitory effect of oridonin on proliferation of U266 cells. The morphological changes of U266 cells were observed under optical microscope. The apoptosis rate of U266 cells was detected by flow cytometry. The mRNA levels of FGFR3, BCL2, CCND1 and MYC genes were quantified by using real-time quantitative PCR method, and the protein levels of BCL2, MYC, CCND1, FGFR3 and P53 were detected by Western blot. The results showed that the oridonin obviously inhibited the growth of U266 cell in dose-and time-dependent manners. As for morphological changes, characteristic apoptotic cells presented in U266 cells treated with 10 µmol/L oridonin for 24 hours. The apoptotic rate of U266 cells increased in dose and time dependent manners; after treatment of U266 cells with oridonin the mRNA levels of FGFR3, BCL2, CCND1 and MYC as well as the their protein levels decreased. Occasionally, the oridonin up-regulated the protein levels of P53 in the same manner. It is concluded that the oridonin can exert its anti-tumor effect by inhibiting proliferation and inducing apoptosis of U266 cell in dose dependent and time dependent manners, that maybe give the clues about new program of target therapy for multiple myeloma.
Antineoplastic Agents
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pharmacology
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Apoptosis
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drug effects
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Cell Line, Tumor
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Cell Proliferation
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drug effects
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Diterpenes, Kaurane
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pharmacology
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Humans
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Multiple Myeloma
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pathology