Objective To explore the histocompatibility and plasticitity of hydroxyapatite/high-density polyethylene composite (HMPE). Design Experimental study. Participants Forty-five rabbits. Methods Rabbits were randomly devided into HA/PE, HA and PE surgical groups, every group included 15 rabbits. Osteoeetomy of orbital rim and implantation (HA/PE, HA and PE materials were used) were performed on 3 groups of the rabbits. At 1st week, 4th week, 8th week, 12th week and 24th week of postoperation, we took out the samples from the rabbits separately in each group and did histopathologic and electron microscope examinations. Main Outcome Measures Physical signs of the implant combination with the bone. The bone cell appeared in the implants. Results After 12 weeks, the calcium salts deposit like bone plate with some osteoblast inside the HA/PE implants was observed. After 8 weeks, the calci- um salts deposit inside the HA plants was observed. In the PE implants, nothing could be seen but some fibrous connective tissue. Conclusion HMPE is an ideal repair material especially for orbital bone.

Animals ; Cochlea ; pathology ; Female ; Guinea Pigs ; Hyperlipidemias ; pathology ; physiopathology ; Male ; Otoacoustic Emissions, Spontaneous

Bone Density Conservation Agents ; therapeutic use ; Bone Neoplasms ; complications ; drug therapy ; secondary ; Breast Neoplasms ; pathology ; Diphosphonates ; therapeutic use ; Female ; Humans ; Male ; Pain ; drug therapy ; etiology ; Prostatic Neoplasms ; pathology ; Quality of Life

Objective To search for the changes of T cells subpopulations and immunoglobulin and their relation-ship in children patients with simple nephrotic syndrome. Design Case-control research. Patients aud Participants 39 patients with simple nephrotic syndrome were divided into two groups:the incipient group and relapse group (6 cases were determined at the incipient and relapse time) .Thereare 28 patients in incipient group, 19 males and 9 females, at the age of 2 to 10 years old. There are20 patients in relapse group, 12 males and 8 females, at the age of 3 to 13 years old. There are 35health children in control group, 21 males and 14 females, 2～13 years old. Interventions T cells subpopulations were determined by indirect immunofluorescence of OKT linesmonoclonal antibodies. The serum IgG was determined by routine simple agar immunodiffusion tests. Results and Conclusions The CD_3~+ and CD_4~+ cells are of no change in the children patients withsimple nephrotic syndrome, and the CD_8~+ and CD_(10)~+ cells are obviously increased, the Values of CD_4~+/CD_8~+ are obviously lower than those in the control qroup, there are no difference between the incipientand relapse groups. The levels of serum IgG were decreased in the 85.3% children patients, IgM were inc-reased in 29.4% of that. The values of CD_4~+/CD_8~+ have positive correlation and negative correlationwith the levels of serum IgG and IgM respectively.

AIMTo investigate the effect of Safflor yellow on the gene expression of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in neonatal asphyxia.

METHODS30 minutes after SY 7 g/kg weight intraperitoneally was administered on the neonatal rats. After asphyxia for 40 minutes,the neonatal rats were reoxygenated for 48 h, and the nitric oxide synthases (NOSs) mRNA expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction.

RESULTSNeuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) were up in hypoxia/reoxygenation (H/R) 48 h group, while both of them were down significantly in SY group, but no change was observed on endothelial nitric oxide synthase (eNOS).

CONCLUSIONThe protective of SY from brain damage induced by neonatal asphyxia might be associated with expression of NOSs mRNA.

Animals ; Brain ; metabolism ; Chalcone ; analogs & derivatives ; pharmacology ; therapeutic use ; Gene Expression ; Hypoxia ; metabolism ; prevention & control ; Nitric Oxide Synthase Type I ; metabolism ; Nitric Oxide Synthase Type II ; metabolism ; Nitric Oxide Synthase Type III ; metabolism ; Quinones ; pharmacology ; therapeutic use ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley

Adult ; Aged ; Aged, 80 and over ; Breast Neoplasms ; complications ; Female ; Humans ; Infusions, Intralesional ; Lung Neoplasms ; complications ; Lymphoma ; complications ; Male ; Middle Aged ; Pericardial Effusion ; etiology ; therapy ; Recombinant Proteins ; administration & dosage ; therapeutic use ; Remission Induction ; Tumor Necrosis Factors ; administration & dosage ; therapeutic use

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; methods ; Phytotherapy ; Purpura, Thrombocytopenic ; etiology ; therapy

Objective:To explore the semantic priming processing characteristics of children with Chinese developmental dyslexia(DD).Methods:According to scores of the dyslexia checklist for Chinese children, character recognition test and Raven's standard progressive matrices (RSPM), 19 primary school students from a school in Wuqia County of Xinjiang were selected as DD group.Meanwhile, 19 children were selected as chronological-age control(CA) group matching in age and RSPM scores, and 20 children were selected as reading-level control(RL) group according to the character recognition test and RSPM scores.The amplitude of N400 of children in three groups were tested by semantic priming paradigm under the conditions of semantic related, unrelated and pseudo words.Results:The accuracy of the DD group under the conditions of irrelevant semantics((0.55±0.25) vs (0.81±0.16), P<0.05), and pseudo characters((0.43±0.27) vs (0.68±0.26), P<0.05) were significantly lower than those of CA group.There was no difference between DD group and RL group, as well as between RL group and CA group(both P>0.05). There was no significant difference in the response time among the three groups of children under semantic related and unrelated conditions( F=1.23, 2.03, both P>0.05). In terms of ERP data, in semantic related condition, there was no significant difference in N400 amplitude among the three groups ( F=0.55, P>0.05). In semantic unrelated condition, the amplitude of N400 in DD group was smaller than that in CA Group((-5.92±3.70)μV vs (-9.76±3.45)μV, P<0.05), and there was no statistically significant difference between DD group and RL Group((-5.92±3.70)μV vs (-7.41±4.02)μV, P>0.05), as well as between CA gorup and RL group.As to pseudo words condition, children in DD group had a lower amplitude of N400 than those in CA and RL groups ((-6.03±2.92)μV vs (-9.98±3.40)μV, (-8.91±3.40)μV, both P<0.05). While no significant differences were found between CA group and RL group. Conclusion:Children with Chinese DD have cognitive defects in semantic and orthographic processing.The semantic cognitive defects may caused by their low reading level, rather than their inherent defect, while the defect in orthographic processing may be their inherent defect.

Objective To explore the influence of low-selenium diet on expression of selenium-binding protein 1 (SBP1) and selenoprotein P (Sel P) in liver,kidney and brain tissues.Methods C57BL/6 mice were randomly divided into four groups according to body weight:control group,low-selenium treatment for 4-,12-and 24-week groups,10 mice in each group,half male and half female.The control group was fed with normal diet (selenium content was 0.300 mg/kg),distilled water,and sacrificed at the 12th week; low-selenium treatment groups were fed with low-selenium diet(selenium content was 0.015 mg/kg),then sacrificed at the 4th,12th and 24th weeks,respectively.Expressions of SBP1 and Sel P in mouse liver,kidney and brain tissues were determined by Western blotting.Results Expressions of SBP1 and Sel P in low-selenium feed mouse liver tissue at the 4th,12th and 24th weeks were,respectively,as follows 0.11 ± 0.01,0.36 ± 0.01,0.59 ± 0.02 and 0.41 ± 0.01,0.39 ± 0.02,0.25 ± 0.02;in kidney,respectively,as follows 0.60 ± 0.03,0.20 ± 0.02,0.03 ± 0.01 and 0.88 ± 0.01,0.73 ± 0.03,0.85 ± 0.02; in brain,respectively,as follows 0.54 ± 0.03,0.11 ± 0.01,0.01 ± 0.01 and 0.50 ± 0.02,0.49 ± 0.03,0.38 ± 0.02.Expression of Sel P in low-selenium feed mouse liver,kidney and brain tissues was significantly decreased as compared to that of control group(1.00 ± 0.00,1.00 ± 0.00,all P ＜ 0.05),but SBP1 content was reduced at first and then rebounded in kidney,and was in decreasing trend in liver and brain tissues.Conclusion Low-selenium diet has a certain effect on expression of SBP1 and Sel P in mouse liver,kidney and brain tissues.

Objective To study the mechanism of mast cell increase in cellular leiomyoma of uterus.Methods Tissue sections from 30 cases of cellular leiomyoma of uterus,15 cases of leiomyosarcoma and 30 cases of ordinary leiomyoma were studied using immunohistochemical double labeling techniques.The expression of mast cell tryptase ahd Ki-67 as well as mast cell tryptase and chemotactic factors RANTES,Eotaxin,monocyte chemoattractant protein-1(MCP-1),transforming growth factor-? (TGF-?)were double immunostained.Results Ki-67 in mast cells was rarely expressed in each group. Expressions of regulate upon activation normal T cell expressed and secreted(RANTES),Eotaxin and TGF-? in cellular leiomyoma were 78%,89%,91%,respectively.They were all higher than those in ordinary leiomyoma and leiomyosarcoma(P