Cone-bine CT(CBCT)images of 2 130 maxillary third molars were divided into 3 groups according to the impaction position. The relationship between the floor of maxillary sinus and the roots of maxillary third molar was analyzed and compared.In low,median and high impaction groups the probability of the root tip of maxillary third molar higher than the floor of maxillary sinus was 4.5%,10.3% and 14.6% respectively.

Since haemoplasmodium is not detected for screening in blood donor,morbidity of transfusion-associated malaria increased rapidly in China recently and became an important problem of public health.It is necessary to emphasize donor screening for malaria.Hematology analyzer with MAPSS is recommended to screen malaria.Flow cytometry and immunochromatography test(ICT)can also screen malaria effectively.For the suspicious samples with haemoplasmodium,it is necessary to be reexamined by standard microscopic examination or polymerase chain reaction(PCR).

[Objective]To discuss the effective method of idiopathic scoliosis and evaluate the result of screw-rod and screw-hook-rod system.[Method]From January 2000 to January 2004,85 cases with idiopathic scoliosis were treated by CD-Horizon instrument.Fifty-one cases were corrected with pedicle screws system only(group A),34 cases were applicated with pedicle hooks or vertebral plate hooks in thoracic vertebra,while with pedicle screws system in lumbar vertebrae(group B).The Cobb's angle of coronal plane and sagittal plane and horizontal plane malformation were compared in preoperative score,postoperative score and the score at 2 years follow-up.[Result]The mean followed-up duration of 85 cases was three years and a half.The coronal plane and sagittal plane correction rate of group A was higher than that of group B at postoperative and 2-year follow-up(P0.05).[Conclusion]Using CD-Horizon instrument is a very effective choice for patients with idiopathic scoliosis.Pedicle screw-rod system offers a significantly better curve correction compared with screw-hook-rod system.

OBJECTIVE:To provide a reference for reducing adverse events due to off-label use of drugs and promoting ratio-nal use of intravenous drugs in Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine. METHODS:The pre-scriptions of intravenous drugs in 42 wards of our hospital from Jul. 2013 to Jun. 2014 were reviewed,and then off-label use of drugs were analyzed,where statistics was made mainly to drug overdose,the use of drugs at the concentration beyond the standards (including concentrations too high and too low) and associated conditions. RESULTS:There were 622 irrational prescriptions,in-cluding 60 concerning off-label use of drugs,accounting for 9.6% of irrational prescriptions,in which 40 were involved with the concentration which was too high,accounting for the highest percentage(66.7% of prescriptions related to off-label use of drugs), 17 were related to drug overdose(28.3% thereof),and 3 were in relation to the concentration which was too low(5.0% thereof). CONCLUSIONS:Pharmacy intravenous admixture service may,through the communication between the pharmacist and the doc-tor,refuse to make up the above-mentioned prescription which was found to be irrational(including that involving in off-label use of drugs)or make up such prescription after it is modified according to the requirements as specified in the label to reduce the occur-rence of off-label use of drugs so as to promote rational drug use.

Objective To observe the effects of naloxone in combination with morphine on the growth of subcutaneous tumor of human gastric cancer MGC-803 cells in nude mice.Methods The model of subcutaneous tumor of human gastric cancer MGC-803 cells in nude mice was established.Fifty nude mice were randomly divided into 5 groups: control group (group C),normal saline group (group S),20 mg/kg morphine group (group M),and 1 mg/kg naloxone group (group N),and 1 mg/kg naloxone+20 mg/kg morphine group (group NM).The mice in group C received no treatment,while the mice in group S,group M,group N and group NM were injected with 1.5 ml/kg saline,20 mg/kg morphine,1 mg/kg naloxone,and 1 mg/kg naloxone+20 mg/kg morphine per day,respectively.The caliper was used to measure the tumor sizes every the other day.The mice in each group received intraperitoneal injection of the drugs for 2 week.Then the relative volume (RTV) of tumor was calculated.The expression of Cyclin D1,VEGF,MMP-9 mRNA and proteins were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR),immunochemistry staining and Western blot.Results RTV in group M (2.21±0.62)% was significantly lower than that in group C (3.16±0.68)%,group S (2.98±0.61)%,group N (3.16±0.35)% and group NM (2.64±0.37)% (P<0.05).RTV in group NM was significantly lower than that in group C,group S and group N (P<0.05).Compared with group C,the expression of Cyclin D1,VEGF,and MMP-9 in group M were significantly decreased (P<0.05).The organizational structure of the subcutaneous tumor in groups C,S,N and NM was almost normal.Cytoplasm vacuolization,disruption of nuclear membrane and chromatin margination were occured in group M.While the level of Cyclin D1,VEGF,and MMP-9 in group NM was increased compared to group M (P<0.05).Conclusion Morphine could inhibit the growth of subcutaneous tumor of human gastric cancer MGC-803 cells in nude mice by downregulating the expression of Cyclin D1,VEGF,and MMP-9.Naloxone could antagonize the anti-growth effects of morphine.

Objective To study the expression of P27,c-fos protein in neuron cell after ischemia and reperfusion accompany with the DWI imagings in rat brain.Methods 12 healthy SD rats with MACO were divided into three groups.Group A,ischemia 30 minutes with 60 minutes reperfusion;Group B,ischemia 60 minutes with 60 minutes reperfusion.Group C,ischemia 60 minutes.The DWI imaging was scanned after surgery immediately.The expressions of P27 and c-fos were determined by immunochemistry procedure.Results The P27 protein positive reaction was not detected in all ischemia and reperfusion groups.The c-fos protein positive reaction was observed in the cortex and basal ganglion in all groups.The hyperintense signal was presented the in DWI after fifteen minutes following the ischemia.Conclusions P27 protein is endogenous cyclin dependent kinase inhibitor.There may be the relationship between low expression of P27 protein in rat brain and the programmed cell death in neuron after the ischemia.C-fos protein participates in the neuron cell injury after the ischemia.DWI has good sensitivity to acute cerebral ischemia.

The evolution of the quality control concepts of medical products within the global context and the development of the quality control technology of Chinese medicine are briefly described. Aimed at the bottlenecks in the regulation and quality control of Chinese medicine, using Big Data technology to address the significant challenges in Chinese medicine industry is proposed. For quality standard refinements and internationalization of Chinese medicine, a technological innovation strategy encompassing its methodology, and the R&D direction of the subsequent core technology are also presented.
Data Mining ; Databases, Factual ; Drug Industry ; organization & administration ; standards ; Drugs, Chinese Herbal ; analysis ; pharmacology ; standards ; Humans ; Quality Control

Cells, Cultured ; Fibroblasts ; cytology ; drug effects ; metabolism ; Humans ; Lung ; cytology ; metabolism ; Macrophages, Alveolar ; metabolism ; Matrix Metalloproteinase 2 ; metabolism ; Matrix Metalloproteinase 9 ; metabolism ; Silicon Dioxide ; toxicity ; Silicosis ; metabolism

Objective To assess the clinical efficacy and safety of the combination of intracoronary tirofiban infusion(ICTI) plus percutaneous coronary intervention(PCI) in patients with acute ST-elevation myocardial infarction (STEAMI). Methods The 128 cases with STEAMI were enrolled in this study. They were randomly divided into trial group and control group. The 10 μg/kg tirofiban were infused into the infarct related artery (IRA) within 5 minutes through the cather after coronary angiography in trial group (n=64). Normal saline in matched dose was infused into IRA after coronary angiography in control group (n=64). The coronary thrombosis and revascularization status were assessed within 10 minutes after injection. Postoperative bleeding complications were observed in all cases. Adverse cardiovascular events and cardiac function were followed up within 1 month in all cases. Results There were 33 cases whose thrombus burden was reduced within 10 minutes after the infusion of tirofiban in trial group, including 26 cases of thrombolysis in myocardial infarction (TIMI) ≥1. There were 6 cases whose thrombus burden was reduced within 10 minutes after the infusion of normal saline in control group, including 3 cases TIMI ≥ 1. The coronary thrombosis and revascularization status were better in trial group rather than in control group (P＜0.01). Adverse cardiovascular events occurred in 5 cases within 1 month, including 2 cases in trial group and 3 cases in control group (P＞0.05). New York heart association functional class and ejection fraction values were better in trial group rather than in control group within 1 month (P＜0.05). Postoperative bleeding complications occurred in 14 cases by TIMI criteria , including severe and mild bleeding in 2 cases in trial group and 1 cases in control group (P＞0.05), but no significant bleeding occurred in 8 cases in trial group and in 3 cases in control group (P＜0.01). Conclusions The combination of intracoronary infusion of tirofiban plus PCI is effective and safe for thrombolysis and revascularization in IRA in patients with STEAMI.

Objective To investigate the effects of NB-UVB on the expression of Gadd45α as well as cell apoptosis and cycle of human HaCaT keratinocytes.Methods Cultured HaCaT cells were exposed to various doses (100,200,400 mJ/cm2)of NB-UVB followed by an additional culture of 6,12 and 24 hours,respectively.Reverse transcription PCR and Western blot were performed to detect the mRNA and protein expression of Gadd45α respectively in HaCaT cells,cell counting kit 8 (CCK8)to measure the proliferation of cells,and flow cytometry to determine the cell cycle distribution of HaCaT cells before and after the exposure to NB-UVB.Results Gadd45α was expressed in HaCaT cells.After exposure to NB-UVB of the three doses,the mRNA and protein levels of Gadd45α increased at 6 hours and 12 hours,but declined at 24 hours,and significant changes were observed in HaCaT cells at the three time points after exposure to NB-UVB of the three doses (all P<0.05).The Gadd45α/β-actin mRNA ratio was 1.4360±0.6551.1.8633±0.0979,1.9266±0.1724 in HaCaT cells 12 hours after irradiation to NB-UVB of 100,200 and 400 mJ/cm2,respectively,significantly higher than that in unirradiated cells(0.6000±0.1276,all P<0.05).Also,increased Gadd45α/β-actin protein ratio was noted in HaCaT cells 12 hours after irradiation to NB-UVB of 100,200 and 400 mJ/cm2 compared with unirradiated cells (0.0773±0.0005,0.1936±0.0015,0.2373±0.0015 vs.0.0290±0.0010,all P<0.05).NB-UVB inhibited the proliferation of HaCaT cells in a time-and dose-dependent manner.Flow cytometry showed that irradiated HaCaT cells were blocked in G2 phase of the cell cycle.and the percentage of HaCaT cells in G2 phase was 13.53%±1.03%,17.77%±2.25%,30.03%±4.29%afler exposure to NB-UVB of 100,200 and 400 mJ/cm2,respectively,compared to 9.24%±0.97%in unirradiated cells (all P<0.05).Conclusions The expression of Gadd45α is increased in HaCaT cells after exposure to NB-UVB,and Gadd45α may be involved in the NB-UVB-induced suprression of cell proliferation of and cell cycle arrest in HaCaT cells.