1.Comparison of SRAP and RAPD markers for genetic analysis of plants in Dendrobium Sw.
Honghong FAN ; Tingchun LI ; Jing QIU ; Yi LIN ; Yongping CAI
Chinese Traditional and Herbal Drugs 1994;0(04):-
Objective This study was carried out in order to analyze genetic diversity in plants of Dendrobium Sw. and compare the marker index (MI) between SRAP and RAPD markers. Methods Using 40 SRAP primer combinations and 36 RAPD primers,the genetic diversity of the materials in Dendrobium Sw. was amplified. Results A total of 1 977 loci were generated by 40 SRAP primer combinations,of which 90.2% was polymorphic and the genetic similarity (GS) ranged from 0.330 2 to 0.789 2. As for RAPD,281 bands were obtained,in which 87.1% was polymorphic,and the GS was from 0.494 2 to 0.773 1. There were some differences existing in the two cluster results revealed by SRAP and RAPD markers,they were as follows:compared to that of RAPD,the result of SRAP could more comfortably reflect the kinship and geographical origin,simultaneously conform to the traditional classification. For the experiment materials,SRAP (16.46) got an MI value 4.5 folds higher than that of RAPD (3.67),which meant SRAP markers were more effective in detecting genomic polymorphisms in the plants of Dendrobium Sw. Conclusion These results show that SRAP and RAPD could be applied to detecting genetic diversity analysis of plants in Dendrobium Sw. SRAP Also shows great superiority and advantage in this study.
2.Effects of apolipoprotein A5 on the metabolism of serum lipid in type 2 diabetic patients
Jing CHANG ; Huan-Qin CHEN ; Lei QIU ; Yi-Wen YANG ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
Apolipoprotein A5(ApoA5)level and other indices were determined in patients with type 2 diabetes mellitus and healthy individuals.Compared to control group,ApoA5 level in the diabetic group was lower (P
3.Development of liquid bandage used for emergency care of war wound
Yingchun XUE ; Yawei WANG ; Bo YANG ; Jing LIU ; Mei LIU ; Yi LU ; Jing DUAN ; Yan QIU
Chinese Medical Equipment Journal 2017;38(5):14-17,28
Objective To develop a liquid bandage for self aid of war wound.Methods Solution A and B were prepared containing functional polymers derived from the biocompatible poly (ethylene glycol) and hyaluronic acid,which had ability of fast crosslinking reaction when they were mixed.Among them,A solution mainly comprised of mercapto group-containing hyaluronic acid derivative (HA-SH),water-solubility starch,propylene glycol,benzoic acid and etc.B solution contained pentaerythritol tetraacrylate derivative (4arm-PEG-Acrylate) and benzoic acid.The structure of mercapto group-containing hyaluronic acid derivative was confirmed by IR,1H-NMR and etc.HA-SH and 4arm-PEG-Acrylate were formulated to different concentrations (W/V) in different buffers.The two kinds of solution were mixed in different ratios,and in situ crosslinking hyaluronic acid hydrogel was obtained;the crosslinking time was recorded.The adhesive force and waterholding capacity of the hydrogel after crosslinking were measured by adding excipients such as water-solubility starch.Results In case the concentration of HA-SH and 4arm-PEG-Acrylate was 2% and pH value was 11,the hydrogel film on wound skin would be quickly formed in 20 seconds for wound dressing after spraying A and B solution onto the wound skin.Conclusion The liquid bandage has strong adhesive force,high water-holding capacity and controllable crosslinking time,so it could be used as a novel wound dressing for war wound.
4.The profile of Th17/Treg balance in the peripheral blood of patients with systemic lupus erythematosus
Haiyan YOU ; Jing LI ; Zhijun JIAO ; Yi WANG ; Yingying QIU ; Ling WU ; Donghai ZHENG
Chinese Journal of Rheumatology 2012;16(1):50-52
ObjectiveTo investigate the profile of Th17/Treg balance in the peripheral blood of patients with systemic lupus erythematosus (SLE).MethodsThirty-two SLE patients in active disease were selected and 30 SLE patients in remission were included in this study.The control group was consisted of 25healthy individuals.The expressions of IL-17 and FoxP3 on CD4+ T cells in the peripheral blood were assessed by flow cytometry and the mRNA levels of these two cytokines were examined by quantitative PCR respectively.ANOVA was used for statistical analysis.ResultsThe percentage of CD4+IL-17+ T cells and IL-17mRNA expression of the active group were significantly higher than those of the remission group and control group[(l.0l±0.22)%,(2.04±0.63)vs (0.48±0.16)%,(1.12±0.34) vs (0.41±0.12)%,1; P<0.01].There was no difference between the remission group and control group(P>0.05).However,the percentage of CI4+FoxP3 + T cells and FoxP3 mRNA expression of the active group were significantly lower than those of the remission group and control group [(2.36±t0.54)%,(0.42±0.16) vs (4.34±0.95)%,(0.87±t0.28) vs (5.09±11.17)%,1; P<0.01 ],and there was also significant differencesbetween the remission group and control group(P<0.05).ConclusionThl7/Treg balance shift may exist in the peripheral blood of patients with SLE and the degree of imbalance may be related to disease activity of SLE.
5.Emerging targets and drugs of inflammatory bowel disease
Chang-wei CHAI ; Yi-xiang ZHANG ; Hai-jing ZHANG ; Lian-qiu WU
Acta Pharmaceutica Sinica 2022;57(5):1282-1288
Inflammatory bowel disease (IBD) is a chronic, repeated intestinal inflammatory disease. Clinically commonly used therapeutic drugs have some disadvantages, such as poor efficacy and many adverse reactions after long-term application. Although new biological therapies such as anti-tumor necrosis factor agents, overcome common adverse reactions, also have problems such as high price, difficult storage, drug resistance and recurrence after application. In recent years, many new therapeutic methods for inflammatory bowel disease have emerged, for example, modulators that inhibit lymphocyte migration (integrin inhibitors and sphingosine 1-phosphate receptor agonists) have been introduced into the clinical treatment of inflammatory bowel disease, inflammatory cytokine inhibitors (interleukin-23 inhibitors, Janus kinase inhibitors, phosphodiesterase inhibitors, etc
6.Epidemiological study of occupational diseases in Guangzhou City, China in 2001 - 2010.
Qiu-hong LIN ; Yi-min LIU ; Jing-yi GUO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(10):759-762
OBJECTIVETo analyze the epidemiological characteristics of occupational diseases in Guangzhou City, China in 2001 - 2010, and to provide a scientific basis for the prevention and control of occupational diseases.
METHODSStatistical analysis was performed on the reported data on the occupational diseases in Guangzhou from 2001 to 2010.
RESULTSA total of 380 cases of occupational diseases (37 types,8 classes) were diagnosed and reported in 2001 - 2010. The three most frequent diseases were occupational poisonings (48.9%), pneumoconiosis (20.0%), and occupational ear, nose, and throat (ENT) diseases (18.7%). In the cases of occupational poisonings, 25.3% suffered from acute occupational poisonings, especially the poisoning by dichloroethane (19.1%), and 74.7% suffered from chronic occupational poisonings, mainly caused by lead (38.8%), benzene (37.4%), and n-hexane (18.0%). The cases of silicosis accounted for 77.6% of all cases of pneumoconiosis. The cases of noise-induced deafness accounted for 97.2% of all cases of occupational ENT diseases. Patients with occupational diseases were reported in 13 districts of Guangzhou, mostly in Panyu (18.9%), Tianhe (13.2%), Baiyun (12.9%), and Liwan (11.1%). All the cases occurred in 19 industries, mainly including light industry (27.1%) and machinery industry (23.7%). Of all cases, 29.5% were in the public sector, 40.3% in the private sector, and 30.3% in the foreign-funded enterprises and those funded by businessmen from Hong Kong, Macao, and Taiwan. The ratio of male to female cases was 15:4. The mean age of onset was 42.2 ± 10.9 years. The average speed of development of occupational diseases was 134.0%, and the average growth rate was 34.0%.
CONCLUSIONThe incidence of occupational diseases tends to increase year by year, with younger age of onset and shorter history of exposure in patients. The occupational poisonings caused by lead, benzene, n-hexane, and dichloroethane, silicosis, and noise-induced deafness seem to be the main occupational hazards in Guangzhou, China. Occupational diseases occur in all districts of Guangzhou and in various industries. The private sector and small enterprises should be the focuses of occupational health supervision.
Adolescent ; Adult ; Aged ; China ; epidemiology ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Occupational Diseases ; epidemiology ; Young Adult
7.Germination and propagartors of artificial seeds of Dendrobium huoshanense.
Ziqing QIN ; Ting ZHAO ; Jing QIU ; Yi LIN ; Yongping CAI
Chinese Journal of Biotechnology 2008;24(5):803-809
The artificial seeds of Dendrobium huoshanese was produced with Auxiliary buds, Protocorm-like bodies, and adventitious shoots. By using orthogonal experiment, we studied the effect of Maltose (%), hormone rate between 6-BA (mgx L(-1)) and NAA (mgL(-1)), active carbon (%), sodium alginate (%), time of ion exchange (min) on germination rate of artificial seeds of D. huoshanese. Then the leaking rate of maltose and variation of pH value of artificial seed capsule during vegetating of artificial seeds of D. huoshanese was measured. The results show that maltose played the most important role in inducing D. huoshanese artificial seeds to germinate. The optimal combination was: maltose 4%, hormone rate between 6-BA (mg x L(-1)) and NAA (mg x L(-1)) 10:1, active carbon 0.1%, sodium Alginate 4%, time of ion exchange is 10 min. Protocorm-like bodies were appropriate propagartor, the germination rate of artificial seeds of D. huoshanese takeing Protocorm-like bodies as the propagartors is 90.1%. After germination, the survival rate of seedlings of artificial seeds was 80.6%, the leaking rate of maltose of artificial seed capsule was 0.52%, and the pH value of artificial seed capsule decreased during the process of vegetation of artificial seeds. After having been stored at 4 degrees C for 20 d, the germination rate of artificial seeds of D. huoshanese takeing Auxiliary buds, Protocorm-like bodies, Adventitious shoots as the propagartors were 3.3%, 10.6%, 5.2%. Under natural conditions the germination rate was 13.8% after 10.0 g/L carbendazim was appended into artificial seed capsule. This result provides a foundation of manufacture and further study of the artificial seeds of D. huoshanese.
Alginates
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pharmacology
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Charcoal
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pharmacology
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Culture Techniques
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methods
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Dendrobium
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growth & development
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Germination
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Glucuronic Acid
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pharmacology
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Hexuronic Acids
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pharmacology
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Maltose
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pharmacology
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Plant Shoots
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growth & development
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Seeds
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growth & development
8.Malignant glomus tumor of bone: report of a case.
Kun-kun SUN ; Da-he XIE ; Qiu-jing SONG ; Dan-hua SHEN ; Hua-yi QU ; Song-lin LIAO
Chinese Journal of Pathology 2007;36(3):215-216
Adolescent
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Bone Neoplasms
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Follow-Up Studies
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Glomus Tumor
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metabolism
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pathology
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surgery
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Humans
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Immunohistochemistry
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Male
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Melanoma
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pathology
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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Tibia
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metabolism
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pathology
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surgery
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Vimentin
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metabolism
9.Characteristic of endoscopic submucosal dissection in elderly patients aged more than or equal to 80 years
Wang JI-HENG ; Zhang JING-YI ; Su HUI ; Sheng JIAN-QIU
China Journal of Endoscopy 2017;23(12):103-105
Objective To analyze the characteristic of endoscopic submucosal dissection (ESD) in elderly patients aged more than or equal to 80 years. Methods We retrospectively reviewed 41 patients aged more than or equal to 80 years with 47 lesions treated by ESD from September 2010 to January 2017. The diseased region, pathology and complications were evaluated. Results Of the 47 lesions, there have 3 esophageal lesions, 21 gastric lesions, 10 colonic lesions and 13 rectal lesions. For the pathology, there have 34 early cancer or precancerous lesion, 2 GIST, 1 neuroendocrine tumor and 10 other types of pathology. 1 cardia1 early cancer underwent additional laparoscopic lymphadenectomy. There have 7 complications, 4 post-ESD bleeding, 2 delirium and 1 pneumonia. Conclusion ESD for colorectal lesion is more frequently in elderly patients aged more than or equal to 80 years. Post-ESD bleeding is the main complication.
10.Relationship of bleeding complications and impairment of draining veins after ?-n-butyl cyanoacrylate embolization of brain arteriovenous malformations
Bing FANG ; Tie-Lin LI ; Chuan-Zhi DUAN ; Yi-Rong WANG ; Qiu-Jing WANG ; Qing-Ping ZHAO ;
Chinese Journal of Radiology 2001;0(07):-
Objective To investigate the causes,consequences and management of injuries to the draining veins after embolization of brain arteriovenous malformations(BAVMs)with ?-n-butyl cyanoacrylate (NBCA).Methods The angiographic imaging data of 189 BAVMs patients who underwent NBCA embolization were studied retrospectively.The status of the draining veins before and after NBCA embolization was observed and compared.The intracerebral hemorrhage(ICH)complications and their relation to their angiographic features were analyzed.Results Twenty-three patients out of 189 patients showed injuries to the draining venous system,including 10 low-grade injury,6 moderate injury,and 7 high- grade injury.Six patients suffered from ICH after embolization,of whom 4 patients were due to injuries of the draining veins(2 moderate and 2 high-grade).In the 3 months follow-up evaluation of 4 patients with ICH, one died,one was in vegetative state,and the other two patients suffered from residual severe or minor (1 patient for each)permanent neurological deficits.Conclusion Our findings suggest that injury of the draining veins is the major cause of ICH and may lead to serious consequences after embolization of BAVMs with NBCA.