Asthma, Occupational ; therapy ; Drainage ; methods ; Humans ; Sputum

Alveolitis, Extrinsic Allergic ; Female ; Humans ; Librarians ; Middle Aged

Adult ; Humans ; Male ; Middle Aged ; Mining ; Molybdenum ; Pulmonary Ventilation ; physiology ; Radiography, Thoracic ; Respiratory Function Tests ; Silicosis ; diagnostic imaging ; physiopathology

Asthma, Occupational ; etiology ; pathology ; Humans ; Inflammation ; pathology ; Neutrophil Infiltration ; Neutrophils ; pathology

Adolescent ; Adult ; Combined Modality Therapy ; Female ; Hexanes ; poisoning ; Humans ; Male ; Medicine, Chinese Traditional ; Occupational Diseases ; therapy ; Treatment Outcome ; Young Adult

Anthrax ; Humans ; Occupational Diseases ; microbiology

Brucellosis ; Humans ; Occupational Diseases ; microbiology

Disaster Planning ; Humans ; Rescue Work

Objective To optimize methods of culturing smooth muscle progenitor cells(SPCs) from mononuclear cells(MNCs) of peripheral blood. Methods Human MNCs isolated from buffy coat were seeded on M-199 with bovine pituitary extraction.On the eighth day outgrowth cells were stimulated with platelet-derived growth factor-BB(PDGF-BB).Fifteen days later,immunofluorescence,Western blot or RT-PCR was used to analyzed the expression of smooth muscle cell specific ?-actin(?-SMA),smooth muscle myosin heavy chain(SM MHC),Calponin,CD34,Tie-2 and Flk-1,and fluorescence activated cell sorter was employed to examine ?-SMA positive cells ratio. Results The cells stimulated by PDGF-BB for 15 d were positive for ?-SMA,SM MHC,Calponin,CD34 and Flk-1,but negative for Tie-2.The ?-SMA positive cells ratio was(90.57?5.63)%,significantly different from that of the control(P

Excessive moisture absorption often occurs in Chinese material medica (CMM) preparations due to its raw material composition is easy to absorb moisture, the increases of water content easily weaken the stability of the preparations and increase the molding difficulty of the products at the same time. At present, the basic principle of moisture absorption can be explained by adsorption theory, theory of capillary condensation, and Fick diffusion theorem, combining a variety of evaluation indexes to judge the degree of moisture absorption, which can be as the basis for developing different moisture-proof technology of CMM preparations. The relevant literatures in recent years were reviewed in this paper, conbined with the widely recognized principle of moisture sorption, characterization method, and feasible moisture-proof technology, aiming to provide technical reference and implementation examples for the moisture-proof of CMM preparations.