3.Identification of the Kiwifruit Germplasms in Jiangxi Province by AFLP and Its Classification Significance
Hua CHEN ; Ganjun YI ; Xiaobiao XU
Chinese Journal of Biochemistry and Molecular Biology 2007;23(2):122-129
The kiwifruit germplasms in Jiangxi province were identified by amplified fragment length polymorphism(AFLP)markers.Four primer pairs that had been selected from 64 ones had detected a total of 190 bands among 31 germplasms of kiwifruit,one hundred and seventy nine bands that representing 94.2%of total bands were polymorphic.The identification rates of 31 germplasms were up to 100%.The result suggests that applying AFLP markers to analyze the kiwifruit germplasms is feasible.Then Clustering analysis the AFLP resuit by UPGMA.The dendrogram indicated that the Dice similarity coefficients of 31 germplasms of kiwifruit ranged from 0.50 to 0.85,it suggested that their genetic relationships weren't near.Thirty one germplasms could divide into four groups according to the Dice similarity coefficient 0.56.Sect.Leiocarpae and Sect.Maculatae clustered into one group;A.Melliana of Sect.Strigosae was one group;A.Chinensis var. Rufopulpa,A.Eriantha,A.Fulvicoma var.Lenata,A.Styracifolia and A.Jiangxiensis in Sect.Stellatae clustered into the same group;but the two germplasms of Sect.Stellatae,A.Deliciosa and A.Chinensis clustered into the other group.Interestingly,the‘Ganmi-5’was in the same rank with A.Chinensis from the dendrogram,but it was the variety of A.Chinensis according to the traditional classification.It is necessary that the‘Ganmi-5’should be further researched in classification.The genetic relationships among the kiwifruit germplasms in Jiangxi province were identified and characterized by themolecular method,which was consistent with the traditional classification in certain degree and provided new evidences for the classification of the kiwifruit germplasms.
4.Reconstructive treatment of blow out fracture in medial orbital wall under nasal endoscope.
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(4):328-330
Adolescent
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Adult
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Endoscopy
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Female
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Humans
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Male
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Middle Aged
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Orbit
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surgery
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Orbital Fractures
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surgery
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Otorhinolaryngologic Surgical Procedures
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Treatment Outcome
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Young Adult
6.Structure relationship of nitrochlorobenzene catalytic degradation process in water over palladium-iron bimetallic catalyst.
Shao-feng NIU ; Hong-yi ZHOU ; Xu-ping AO ; Xin-hua XU ; Zhang-hua LOU
Journal of Zhejiang University. Science. B 2006;7(7):548-552
Two isomers of nitrochlorobenzene (o-, and p-NCB) were treated by a Pd/Fe catalyst in aqueous solutions through catalytic amination and dechlorination. Nitrochlorobenzenes are rapidly converted to form chloroanilines (CAN) first through an amination process, and then rapidly dechlorinated to become aniline (AN) and Cl(-), without the involvement of any other intermediate reaction products. The amination and dechlorination reaction are believed to take place predominantly on the surface site of the Pd/Fe catalysts. The dechlorination rate of the reductive degradation of the two isomers of nitrochlorobenzene (o-, and p-NCB) in the presence of Pd/Fe as a catalyst was measured experimentally. In all cases, the reaction rate constants were found to increase with the decrease in the Gibbs free energy (correlation with the activation energy) of NCBs formation; the activation energy of each dechlorination reaction was measured to be 95.83 and 77.05 kJ/mol, respectively for o- and p-NCB. The results demonstrated that p-NCBs were reduced more easily than o-NCBs.
Catalysis
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Industrial Waste
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prevention & control
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Iron
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chemistry
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Isomerism
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Kinetics
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Metals
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chemistry
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Nitrobenzenes
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chemistry
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Palladium
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chemistry
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Structure-Activity Relationship
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Waste Disposal, Fluid
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methods
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Water
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chemistry
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Water Purification
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methods
7.Administrative internship for training medical students of comprehensive quality
Yi XU ; Qing LIN ; Qing ZANG ; Hua LI ; Ruoyu LUO
Chinese Journal of Medical Education Research 2011;10(12):1422-1424
Medical postgraduates are the future core force of clinical diagnosis and treatment,scientific research,education and sanitary administration.Therefore,to cultivate qualified professionals,the key of contemporary medical postgraduate education is to pay more attention to comprehensive quality training in order to make students adapt to the continuous development of society and medical model.The First Clinical College in Wuhan University has implemented the hospital administration internship in combination with social needs and explored the education model of contemporary medical students with comprehensive quality.
8.Pharmaceutical Care for One Elderly Patient with AECOPD and Baumanii Infection
Nian HUA ; Ma MINGHUA ; Yang LI ; Xu YI ; Zhu QUANGANG
China Pharmacist 2015;(5):791-793,796
Objective:To explore the therapeutic regimen and key points in the pharmaceutical care for the elderly patients with a-cute exacerbation of the chronic obstructive pulmonary disease ( AECOPD) . Methods: On the basis of clinical pharmacist work, one typical case was selected. Referring to the COPD treatment guidelines, the treatment rationality was analyzed. Meanwhile, an individu-alized pharmaceutical care plan was established and carried out in whole process of the treatment. Results:The pharmacotherapy was effective and rational. By providing the pharmaceutical care for the AECOPD patient, the related problems in the treatment were solved promptly, and the rational advice on the drug treatment was provided. Conclusion:It is very important to enhance integrated pharma-ceutical care in the elderly patients with AECOPD.
9.Content Determination of Cabozantinib in Its Raw Material by RP-HPLC
Wei XU ; Changjuan ZHAN ; Hua WANG ; Yi WANG ; Qi GUO
China Pharmacy 2017;28(12):1696-1698
OBJECTIVE:To establish a method for the content determination of cabozantinib in its raw material. METHODS:RP-HPLC method was adopted. The determination was performed on Inertsil ODS-SP C18 column with mobile phase consisted of acetonitrile-0.02 mol/L ammonium acetate buffer (pH 5.2,52:48,V/V) at the flow rate of 1.0 mL/min. Detection wavelength was set at 241 nm,the column temperature was 38 ℃,and sample size was 20 μL. RESULTS:The linear range of cabozantinib were 9.88-49.40 μg/mL(r=0.9999). The limit of quantitation was 11.46 ng,and the limit of detection was 3.36 ng. The RSDs of preci-sion,stability,repeatability tests were all lower than 2.0%;recoveries were 98.5%-101.7%(RSD=1.2%,n=9). CONCLU-SIONS:The method is simple,accurate and suitable for the content determination of cabozantinib in its raw material.
10.Drug Release Comparison of Nifedipine Sustained-release Tablets from Four Manufacturers
Yi WANG ; Changjuan ZHAN ; Wei XU ; Hua WANG ; Yong ZHANG
China Pharmacist 2014;(11):1856-1858
Objective:To compare the drug release of nifedipine sustained-release tablets from four manufacturers to evaluate the intrinsic quality. Methods:The drug release rate was determined by UV respectively with pH 1. 2 HCl solution, pH 4. 0 sodium ace-tate buffer, pH 6. 8 phosphate buffer and water as the dissolution medium. The dissolution curves were compared by f2 factor. Results:The drug release rate of nifedipine sustained-release tablets from the four manufacturers all met quality standard of our country, al-though the dissolution curves in the different dissolution medium was various. Conclusion:There are differences in intrinsic quality a-mong the nifedipine sustained-release tablets from the four manufacturers. The dissolution examination standard should be improved fur-ther.