1.Relationship between genetic polymorphisms of interferon-gamma gene intron 1 +874 site and recurrent Condyloma acuminata.
Acta Academiae Medicinae Sinicae 2009;31(1):3-5
OBJECTIVETo explore the relationship between the polymorphisms of interferon-gamma (IFN-gamma) gene intron 1 at position + 874 and Condyloma Acuminata (CA).
METHODSIFN-gamma gene single nucleotide polymorphisms (intron 1 at position + 874) were detected in 156 subjects, including 76 patients with recurrent CA (CA group) and 80 healthy controls (control group), by polymerase chain reaction with sequence specific primers.
RESULTSNo significant difference of IFN-gamma 1 + 874 was found between CA group (TT, TA, and AA frequencies were 10.5%, 34.2%, and 55.3%, respectively) and control group (TT, TA, and AA frequencies were 7.5%, 30.0%, and 62.5%, respectively) (chi2 = 0.959, P = 0.619).
CONCLUSIONIFN-gamma gene polymorphism (intron 1 at position + 874) is not correlated with recurrent CA.
Adolescent ; Adult ; Aged ; Condylomata Acuminata ; genetics ; Female ; Humans ; Interferon-gamma ; genetics ; Introns ; genetics ; Male ; Middle Aged ; Polymerase Chain Reaction ; methods ; Polymorphism, Genetic ; genetics ; Polymorphism, Single Nucleotide ; Recurrence ; Young Adult
2.Design and clinical application of a three-dimensional biomechanical traction appliance for protrusion of intervertebral disc.
Lei-gang YANG ; Yun-gang YANG ; Xiu-ming YANG ; Zuo-yi LIU ; Huai-xing WEN
Chinese Journal of Medical Instrumentation 2002;26(3):190-191
A three-dimensional biomechanical tracting appliance is introduced in the article, which is used to treat the protrusion of intervertebral disc. The appliance is light, practical, adjustable 3D biomechanic, simple and with multiple functions and convenient operation.
Biomechanical Phenomena
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Equipment Design
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Humans
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Intervertebral Disc Displacement
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therapy
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Lumbar Vertebrae
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pathology
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Traction
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instrumentation
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methods
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Treatment Outcome
3.Recent Advances in Technique and Clinical Outcomes of Minimally Invasive Spine Surgery in Adult Scoliosis
Liu GANG ; Liu SEN ; Zuo YU?ZHI ; Li QI?YI ; Wu ZHI?HONG ; Wu NAN ; Yu KE?YI ; Qiu GUI?XING
Chinese Medical Journal 2017;(21):2608-2615
Objective: Conventional open spinal surgery of adult scoliosis can be performed from anterior, posterior, or combined approach. Minimally invasive spine surgery (MISS) was developed for the purpose of reducing the undesirable effects and complications. This review aimed to make a brief summary of recent studies of the approach and clinical outcomes of MISS in adult scoliosis. Data Sources: We conducted a systematic search from PubMed, Medline, EMBASE, and other literature databases to collect reports of surgical methods and clinical outcomes of MISS in treatment of adult scoliosis. Those reports were published up to March 2017 with the following key terms: "minimally invasive," "spine," "surgery," and "scoliosis."Study Selection: The inclusion criteria of the articles were as followings: diagnosed with adult degenerative scoliosis (DS) or adult idiopathic scoliosis; underwent MISS or open surgery; with follow?up data. The articles involving patients with congenital scoliosis or unknown type were excluded and those without any follow?up data were also excluded from the study. The initial search yielded 233 articles. After title and abstract extraction, 29 English articles were selected for full?text review. Of those, 20 studies with 831 patients diagnosed with adult DS or adult idiopathic scoliosis were reviewed. Seventeen were retrospective studies, and three were prospective studies. Results: The surgical technique reported in these articles was direct or extreme lateral interbody fusion, axial lumbar interbody fusion, and transforaminal lumbar interbody fusion. Among the clinical outcomes of these studies, the operated levels was 3–7, operative time was 2.3–8.5 h. Both the Cobb angle of coronal major curve and evaluation of Oswestry Disability Index and Visual Analog Scale decreased after surgery. There were 323 complications reported in the 831 (38.9%) patients, including 150 (18.1%) motor or sensory deficits, and 111 (13.4%) implant?related complications. Conclusions: MISS can provide good radiological and self?evaluation improvement in treatment of adult scoliosis. More prospective studies will be needed before it is widely used.
4.FAK/c-Src signaling pathway mediates the expression of cell surface HSP90 in cultured human prostate cancer cells and its association with their invasive capability.
Xue-guang LIU ; Ye GUO ; Zuo-qin YAN ; Mu-yi GUO ; Zhi-gang ZHANG ; Chang-an GUO
Chinese Journal of Oncology 2011;33(5):340-344
OBJECTIVETo investigate the expression of heat shock protein 90 (HSP90) on the cell surface of highly invasive human prostate cancer cells PC3 and its possible molecular mechanisms of its effect on cell invasion through analyzing FAK/Src signaling pathway.
METHODSThe expression of cell surface HSP90 on PC3 cells was studied by immunofluorescence staining and surface biotinylation assay respectively. A specific HSP90 antibody was used to inhibit the cell surface HSP90. In vitro cell invasion was assessed by modified Boyden chambers. Phosphorylated FAK on tyr 397, 576, 577 and 925, and phosphorylated c-Src on tyr 416 were examined by Western blot assay. The association between FAK and c-Src was analyzed by immunoprecipitation. The effects of FAK knockdown by siRNA or Src kinases inhibitor PP2, with or without anti-HSP90 antibody, on PC3 cell invasion were also evaluated.
RESULTSA pool of HSP90 was detected on the cell surface of PC3 cells. A specific HSP90 antibody significantly retarded tumor cell invasion. Concomitant with this finding, targeting cell surface HSP90 significantly inhibited the phosphorylations of FAK and c-Src, and also the interactions between FAK and c-Src. FAK knockdown or PP2 dramatically suppressed cell invasion, however, anti-HSP90 antibody didn't further inhibit cell invasion.
CONCLUSIONSCell surface HSP90 promotes human prostate cancer cell invasion through a FAK/c-Src signaling, with may be a novel therapeutic target against metastatic tumors.
Antibodies ; pharmacology ; Cell Line, Tumor ; Cell Membrane ; metabolism ; Focal Adhesion Protein-Tyrosine Kinases ; genetics ; metabolism ; Gene Knockdown Techniques ; HSP90 Heat-Shock Proteins ; immunology ; metabolism ; Humans ; Male ; Neoplasm Invasiveness ; Phosphorylation ; Prostatic Neoplasms ; metabolism ; pathology ; Pyrimidines ; pharmacology ; RNA, Small Interfering ; genetics ; Signal Transduction ; Transfection ; src-Family Kinases ; antagonists & inhibitors ; metabolism
5.Incidence of seroresistance of syphilis and its relevant factors.
Lin-Na WANG ; Ya-Gang ZUO ; Yong-Xin LIU ; Xiu-Rong LIU ; He-Yi ZHENG
Acta Academiae Medicinae Sinicae 2008;30(3):338-341
OBJECTIVETo investigate the incidence of seroresistance of syphilis and analyze its relevant factors.
METHODSThe clinical data of 131 patients with syphilis were retrospectively analyzed. The incidence of seroresistance was investigated and the correlation between seroresistance and factors including age, gender, original titer, disease course, and medications were analyzed.
RESULTSThe incidence of seroresistance was not significantly different among patients with different ages and genders, but was significantly different among patients with different disease courses, antibody titers, and medications. Patients with a baseline serum rapid plasma reagin (RPR) titer of greater than 1: 8, a latent course, or a macrolide therapy history had higher incidences of seroresistance (i. e., 61%, 45.6%, and 72.7% respectively) than those who had a lower RPR titer, a primary course, or a benzathine penicillin therapy history.
CONCLUSIONSyphilis patients with a high baseline RPR titer, a latent course, and a macrolides therapy are prone to be seroresistant.
Adolescent ; Adult ; Aged ; Female ; Humans ; Macrolides ; therapeutic use ; Male ; Middle Aged ; Reagins ; blood ; Syphilis ; blood ; diagnosis ; drug therapy ; immunology ; Syphilis Serodiagnosis ; methods ; Young Adult
6.Analysis of 1125 syphilis cases.
Jun LI ; Lin-na WANG ; He-yi ZHENG ; Ya-gang ZUO ; Yong-xin LIU ; Xiu-rong LIU
Acta Academiae Medicinae Sinicae 2010;32(2):185-189
OBJECTIVETo summarize the epidemiological, clinical and serological features of syphilis.
METHODThe clinical data of 1125 patients with syphilis were retrospectively analyzed.
RESULTSNon-marital sexual intercourse is the main route of infection. The 129 cases of primary syphilis were featured by extragenital chancres and the 357 cases of secondary syphilis were featured by scaling macules on the palms and soles and condylomata lata. Early latent syphilis were detected in 178 cases, late latent syphilis in 174 cases, and latent syphilis of unknown duration in 285 cases. Concurrent infection with other sexually transmitted diseases were noted in 107 cases (9.51%). Most patients were treated with benzathine penicillin, and only 31 patients (2.75%) were allergic to this therapy. Except for the primary syphilis, the sensitivity of syphilis serological tests (including rapid plasma regain test, treponema pallidum particle agglutination, treponema pallidum hemagglutination assay, and fluorescent treponemal antibody-absorption) were higher than 97%.
CONCLUSIONSThe incidence of latent syphilis is high, while the incidence of late syphilis is low. Penicillin remains the treatment of choice for syphilis.
Adult ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Syphilis ; diagnosis ; epidemiology ; therapy ; Young Adult
7.Comparison of clinical efficacy of orthotopic ileal neobladder versus orthotopic sigmoid neobladder
Jian-Song WANG ; Hong-Yi XU ; Yong-Fu SHI ; Hui ZHAN ; Jong-Ming LI ; Ze-Hui LI ; Yi-Gang ZUO ; Delin YANG ; Chao WANG ; Chang-xing KE ; Ming-xia DING ; Ru-ping YAN
Chinese Journal of Urology 2000;0(12):-
Objective To compare the clinical efficacy of orthotopic ileal neobladder versus ortho- topic sigmoid neobladder.Methods The data of 96 patients who had undergone orthotopic ileal neoblad- der and 68 patients who had undergone orthotopic sigmoid neobladder were retrospectively analyzed.The perioperative condition,urinary continence,urodynamics,and pouch-related complications were compared between the 2 groups.Results Of all the 164 patients,12(7.3%)were lost to follow-up.The mean fol- low-up was 46(2-86)months in orthotopic ileal neobladder group,and 42(4-78)months in orthotopic sigmoid neobladder group.There was no significant difference in intraoperative blood loss and postoperative urinary continence between the 2 approaches(P>0.05).However,compared with sigmoid neobladder group,ileal neobladder group had longer operative time and postoperative recovery time,and got a bigger pouch(P<0.05).The early and late pouch-related complication rates of ileal neohladder group were 16. 7% and 29.2%,which were higher than those of sigmoid neobladder group.During the follow-up,tumor recurred in 3 cases of ileal neobladder group,but none in sigmoid neobladder group.Conclusions Ortho- topic ileal neobladder and sigmoid neobladder are similar in operative difficulties,and both can achieve satis- factory clinical results.Compared with ileal neobladder,sigmoid neobladder has shorter operative time, quicker recovery and lower rate of pouch-related complications,thus is a preferred procedure.
8.Effects of ketamine on proliferation and apoptosis of pheochromocytoma cell.
Yuan-Yi ZUO ; Yan-Bo ZHAO ; Xiao-Gang JIANG ; Zhen-Lun GU ; Ci-Yi GUO ; Shi-Zhong BIAN
Journal of Forensic Medicine 2011;27(6):405-412
OBJECTIVE:
To explore the effect of ketamine on adrenal pheochromocytoma (PC12) cell proliferation inhibition and induction of apoptosis and its mechanism.
METHODS:
PC12 cells of rats were models for dopaminergic neuron. PC12 cells were cultured with ketamine at concentrations of 0.9, 1.2, 1.5, 1.8 and 2.1 mmol/L, respectively. The cell viability was measured by MTT method after incubation at 12, 24, 48 and 72h. Hoechst stain was used to observe the morphological changes of apoptosis. PC12 cells cultured after 48 h with different concentrations of ketamine were selected to detect apoptotic rate using flow cytometry and detect the expression of bax and bcl-2 proteins using Western blotting.
RESULTS:
For different concentrations of ketamine, vitality of PC12 cells significantly decreased with increase of the incubation time. Apoptosis was obviously observed using Hoechst staining. Flow cytometry showed that apoptosis rates significantly increased with increasing ketamine concentrations.
CONCLUSION
Ketamine can inhibit the proliferation of PC12 cell by inducing apoptosis of the PC12 cell in a concentrations-dependent manner. The underlying mechanism may be related to promoting the expression of bax and inhibiting the expression of bcl-2 in the cells.
Anesthetics, Dissociative/pharmacology*
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Animals
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Apoptosis/drug effects*
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Blotting, Western
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Cell Proliferation/drug effects*
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Dose-Response Relationship, Drug
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Flow Cytometry
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Gene Expression Regulation/drug effects*
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Ketamine/pharmacology*
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PC12 Cells
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Proto-Oncogene Proteins c-bcl-2/metabolism*
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Rats
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Time Factors
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bcl-2-Associated X Protein/metabolism*
9.Clinical study of laparoscopic sphincter-preserving proctectomy for low rectal cancer using transanal telescopic anastomosis.
Shi-yong LI ; Gang CHEN ; Guang CHEN ; Fu-yi ZUO ; Xiao-jun WEI ; Qiang YUAN ; Jun-feng DU
Chinese Journal of Gastrointestinal Surgery 2011;14(7):532-534
OBJECTIVETo investigate the safety, feasibility and clinical outcomes of laparoscopic sphincter-preserving proctectomy for low rectal cancer using transanal telescopic anastomosis.
METHODSFive patients underwent laparoscopic sphincter-preserving proctectomy for low rectal cancer using transanal telescopic anastomosis between March 2011 and April 2011 at the General Hospital of Beijing Military Command. After lymph node dissection around the mesentery using harmonic scalpel, the root of the inferior mesenteric vessel was ligated and transected. Rectal dissection was further carried out until 5 cm distal to the lower margin of the tumor. A circumferential incision was made 1.0 cm above the dentate line using 5 support stitches for exposure. The submucous layer was striped upward to the level of the levator ani, and rectum was transected. Rectum and sigmoid colon were extracted transanally and removed. Finally, colonanal anastomosis was made using telescopic technique.
RESULTSFive patients underwent the procedure successfully. The mean operative time was 178 minutes. The mean intraoperative blood loss was 76 ml. The mean lymph nodes retrieval was 14. Bowel function recovered after a mean of 3 days. There were no postoperative complications. No obvious scars were seen in the abdomen or the anus. The mean hospital stay was 12 days. After one year of follow-up, all the patients survived cancer-free.
CONCLUSIONSLaparoscopic anterior resection with sphincter preservation by transanal telescopic anastomosis for low rectal cancer is feasible and safe. Abdominal incision is minimal. However, the long-term outcomes require further investigation.
Adult ; Aged ; Anal Canal ; surgery ; Anastomosis, Surgical ; methods ; Feasibility Studies ; Female ; Humans ; Laparoscopy ; methods ; Male ; Middle Aged ; Rectal Neoplasms ; surgery
10.Impact of disease activity on postoperative recurrence and complications after bowel resection for Crohn's disease.
Lu-gen ZUO ; Yi LI ; Hong-gang WANG ; Wei-ming ZHU ; Lei CAO ; Wei ZHANG ; Jian-feng GONG ; Ning LI ; Jie-shou LI
Chinese Journal of Surgery 2012;50(8):695-698
OBJECTIVETo determine whether the perioperative disease activity is associated with recurrence and complications after bowel resection for Crohn's disease (CD).
METHODSClinical data of patients underwent bowel resection for CD at the Nanjing General Hospital of Nanjing Military Command from January 2002 to January 2011 was retrospectively analyzed. Postoperative recurrence and complications in patients with active disease were compared with those in patients with remission.
RESULTSA total of 90 patients underwent bowel resection for CD, active disease were seen in 43 patients at the time of surgery, while the rest 47 patients were in remission. The postoperative cumulative endoscopic recurrence rate was 8.5% at 1 year, 27.7% at 2 years and 44.7% at 3 years in the patients with remission, and was 27.9% at 1 year, 37.2% at 2 years and 53.5% at 3 years in patients with active disease. Data indicated the endoscopic recurrence were statistically significant in the first year after surgery (χ² = 4.605, P = 0.032). Additional, the postoperative complication rates in patients with remission (14.9%) was significantly lower than that in patients with active disease (51.2%) (χ² = 6.979, P < 0.001).
CONCLUSIONPatients with active disease at the time of surgery were encountered with early postoperative recurrence and increased complications after intestinal resection for CD.
Adult ; Colon ; surgery ; Crohn Disease ; physiopathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Male ; Postoperative Complications ; Recurrence ; Retrospective Studies ; Young Adult