Objective To compare the effects of 2 kinds of frequency of transcutaneous electrical nerve stimulation (TENS) on urinary incontinence after stroke. Methods 81 patients with post-stroke urinary incontinence were randomized into group A (n=27), group B (n=27) and group C (n=27), who accepted TENS (biphasic square waves with pulse durations of 150 μs) in 20 Hz, 75 Hz and medcine only, respectively, for 90 days. They were assessed with Overactive Bladder Symptom Scores (OABSS), Barthel Index (BI), and voiding diary before and after treatment. Results The OABSS, BI and voiding diary improved significantly after treatment (P<0.05) in groups A and B, and improved more in the group A than in the group B. There was not significant improvement in the group C (P>0.05). Conclusion TENS of 20 Hz is more effective to reduce the incontinence post stroke.

Air ; Animals ; Diving ; Male ; Rats ; Rats, Sprague-Dawley ; Reactive Oxygen Species ; metabolism ; Spleen ; metabolism

Objective To study the effect of hypoxia-ischemia (HI) on the brain-derived neurotrophic factor (BDNF) expression in the brain cortex and the hippocampus of immature rats,and to provide new therapeutic strategies for HI brain injury.Methods Three-day-old rats were divided into 2 groups.One group of rat pups were subjected to the left carotid artery ligation followed by 60 mL/L oxygen for 2.5 hours(HI-treated rats).The other group of rat pups were only subjected to the left carotid artery separation without ligation and 60 mL/L oxygen (sham-treated rats).The brain tissues were prepared at 3,7 and 14 d after treatment.Cresyl fast videt(CV) staining was used to evaluate the damage of the cortex and the hippocampus and check whether the models were successfully made.Immunostaining was used to determine the changes in BDNF positive cells in the brain cortex and the hippocampus after HI.Western blot analysis was used to evaluate the expression of BDNF protein in the brain cortex and the hippocampus after HI.Results Models were successfully made.CV staining showed that there was brain damages and area loss in the cortex and the hippocampus after HI.BDNF immunostaining showed that the number of BDNF-positive cells was significantly decreased in the cortex (t =-3.225,-2.298,all P ＜ 0.05) and the hippocampus (t =-3.751,-2.920,all P ＜ 0.05) in the damaged side of the brain compared to the contralateral side in the rats treated with HI and the sham-treated rats at 3 d after surgery,while increased at 7 d(t =3.924,2.838,all P ＜ 0.05 for cortex ; t =4.136,2.256,all P ＜0.05 for hippocampus) and 14 d (t =3.256,2.624,all P ＜ 0.05 for cortex ; t =3.051,2.719,all P ＜ 0.05 for hippocampus) after surgery.Western blot analysis showed protein expressions of BDNF:(1) Hippocampus:the protein expressions of BDNF were significantly decreased in damaged side of the brain compared to the contralateral side of rats treated with HI at 3 d(t =-3.388,P ＜ 0.05) after surgery,while increased compared to the contralateral side of rats treated with HI and the sham-treated rats at 14 d(t =4.874,4.646,all P ＜0.05) after surgery.(2)Cortex:the protein expression of BDNF was significantly decreased in damaged side of the brain compared to the contralateral side of rats treated with HI and the sham-treated rats at 3 d(t =-7.386,-3.256,all P ＜ 0.05) after surgery,compared to the sham-treated rats at 7 d(t =4.439,P ＜ 0.05) and the contralateral side of rats treated with HI and the sham-treated rats 14 d(t =24.161,3.942,all P ＜ 0.05) after surgery.Conclusions The number of BDNF-positive cells and protein expressions are decreased in the cortex and the hippocampus at the early stage of HI injury,and increased at the late stage.BDNF may play a role in the healing stage of HI brain injury.

Objective To evaluate the effects of dexmedetomidine on the activity of nuclear factor kappa B (NF-κB) in neutrophil granulocytes during one-lung ventilation in the patients undergoing pulmonary lobectomy.Methods Thirty-eight ASA physical status Ⅰ or Ⅱ patients,aged 40-64 yr,weighing 50-75 kg,scheduled for elective pulmonary lobectomy,were randomized into 2 groups (n =19 each) using a random number table:control group (group C) and dexmedetomidine group (group D).In group D,dexmedetomidine 1.0 μg/kg was infused intravenously over 15 min before induction of anesthesia,followed by infusion at a rate of 0.5 μg· kg-1 · h-1 until 30 min before the end of operation.The equal volume of normal saline was given in group C.At 5 min after intubation,0,30 and 60 min of one-lung ventilation,0 and 30 min of two-lung ventilation,and 30 min after operation,blood samples were taken from the radial artery for blood gas analysis and for measurement of plasma concentrations of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).The oxygenation index and respiratory index were calculated.The nuclear protein of neutrophil granulocytes was extracted for measurement of NF-κB DNA-binding activity.Results Compared with group C,the respiratory index,plasma concentrations of TNF-α and IL-6 and NF-κB DNA-binding activity were significantly decreased,and no significant difference was found in the oxygenation index in group D.Conclusion Dexmedetomidine can inhibit the activation of NF-κB in neutrophil granulocytes and is helpful in reducing the systemic inflammatory responses during one-lung ventilation in the patients undergoing pulmonary lobectomy.

Combined Modality Therapy ; Consensus ; Head and Neck Neoplasms ; therapy ; Humans

Health Knowledge, Attitudes, Practice ; Humans ; Neoplasm Staging ; Risk Assessment ; methods ; Thyroid Diseases ; surgery ; Thyroid Neoplasms ; pathology ; surgery ; Thyroidectomy ; adverse effects ; methods

Carcinoma, Squamous Cell ; pathology ; surgery ; Head and Neck Neoplasms ; pathology ; surgery ; Humans ; Lymph Nodes ; pathology ; surgery ; Lymphatic Metastasis ; Minimally Invasive Surgical Procedures ; methods ; Neck ; Neck Dissection ; methods ; Neoplasm Staging

Objective: To investigate the effects of 1,25(OH)_(2)D_(3) on renal interstitial fibrosis in rats.Methods: Male SD rats(7 weeks old) were divided into 4 groups at random: ①the treatment group(6 rats): Unilateral ureteric obstruction(UUO)+1,25(OH)_(2)D_(3) 3 ng/100 g body wt/day intraperitoneally(I.P),②the control group(6 rats): UUO + ethanol by I.P,③UUO group(6 rats),④sham operated group(6 rats).Kidneys were harvested at day 7 after UUO.Fibrosis was assessed by measuring tissue hydroxyproline content.Tissue NF-?B expression was determined by RT-PCR. Results: UUO for 7 days significantly activated NF-?B,induced interstitial fibrosis in obstructed kidneys in the control group and UUO group compared with sham operated group rats.Conversely daily administration of(1,25(OH)_(2)D_(3)) significantly inhibited NF-?B expression,inflammatory cell infiltration and decreased interstitial fibrosis in obstructed kidneys in the treatment group. Conclusion: 1,25(OH)_(2)D_(3) had beneficial effects on interstitial fibrosis in SD rats with UUO,and this effect may be associated with 1,25(OH)_(2)D_(3) inhibits NF-?B expression and inflammatory cell infiltration.

Combined Modality Therapy ; Head and Neck Neoplasms ; drug therapy ; pathology ; radiotherapy ; surgery ; Humans ; Hypopharyngeal Neoplasms ; radiotherapy ; surgery ; Medical Oncology ; education ; Nasopharyngeal Neoplasms ; radiotherapy ; surgery ; Neoplasm Staging ; Otorhinolaryngologic Surgical Procedures ; education

Combined Modality Therapy ; Follow-Up Studies ; Head and Neck Neoplasms ; diagnosis ; epidemiology ; therapy ; Humans ; Quality of Life ; Specialization