Humans ; Infarction, Middle Cerebral Artery/surgery* ; Thrombectomy ; Thrombosis/etiology* ; Stents/adverse effects* ; Treatment Outcome

This text involved with a pre-enrichment medium for the simultaneous recovery of Salmonella spp.,Escherichia coli and Staphylococcus aureus.This medium is named buffered saline broth(BSB),which contains peptone 10g,beef exact 3g,disodium hydrogen phosphate 9g,potassium dihydrogen phosphate 1.5g,additive 50g,deionized water 1 000mL,pH7.2.1cfu per one milliliter of Salmonella spp.,Escherichia coli and Staphylococcus aureus in saline were stimutaneously added to 97 mL of BSB,buffered peptone water,lactose broth,nutrient broth,Escherichia coli broth,rappaport-vassiliadis enrichment broth,7.5% sodium chloride enrichment medium,respectively,and incubated for 18h at 37℃.The results showed BSB was the best enrichment medium,in which Salmonella spp.,Escherichia coli and Staphylococcus aureus multiplied at nearly same speed,and reached at 10~6 、10~6 、10~7 cfu/mL,respectively.Multiplex PCR produced specific amplicons of expected sizes,284bp for Salmonella spp.invA gene,622bp for Escherichia coli phoA gene,484bp for Staphylococcus aureus nuc gene.In contrast,the three bacteria couldn't multiply harmoniously in the other six media.So BSB might be considered as the medium,which could enrich above mentioned three bacteria.

0.05).Conclusion Acupuncture has an effect on I/O curve amplitude of EPSP and PS,and on LTP induction of PS of dentate gyrus(DG) in rats after cerebral ischemia.However,the difference of effect of acupuncture at different doses on LTP induction is insignificant.

Objective To investigate the efficient methods used for tracing endothelial progenitor cells(EPCs)after transplantation in injured lung tissue.Methods Thirty clean grade healthy syngeneic SD female rats were divided into three experimental groups(n =10):(1)sham group,rats treated with intravenous phosphate-buffered saline(PBS)instead of lipopolysaccharide(LPS)followed by EPCs graft;(2)PBS-treated group,rats treated with intravenous PBS after intravenous injection of LPS to produce acute lung injury;(3)EPC-treated group,rats treated with EPCs after intravenous injection of LPS to produce acute lung injury.The transplanted EPCs were got from the same genetic species of the SD male rats.Rats of each group were sacrificed 7 days after EPCs transplantation.Their whole lung tissues were harvested to detect the expression of Y-chromosome by using hybridization in situ and RT-PCR assay.Statistic package of SPSS16.0 was used for the data analysis and significant differences between means were evaluated by ANOVA analysis.Results Compared with the other two groups,positive signals of sex-determining region y were found in lung endothelium from the EPC-treated group.Conclusions Y chromosme specific probe can be one of efficient methods for tracing stem cells after transplantation.

Humans ; Aortic Dissection/surgery* ; Aortic Rupture/surgery* ; Death ; Treatment Outcome

Pregnancy ; Humans ; Female ; Cesarean Section/adverse effects* ; Abdominal Cavity ; Neoplasms

Objective To investigate reliability and validity of the Chinese version of the schedule for assessment of insight (SAI).Methods The original version of SAI was translated into Chinese and then backtranslated into English to develop the Chinese version of SAI which reliability and validity were tested.220 subjects who met the diagnosis of Schizophrenia,schizophreniform disorder,schizoaffective disorder,delusional disorder or brief psychotic disorder were assessed.Results The Cronbach's α was 0.890 ; the test-retest reliability was 0.742 ; the inter-rater reliability was 0.986.Three main factors were obtained from the factor load matrix as awareness of illness,ability to relabel psychotic experience,compliance with treatment.The loading coefficient were 0.883-0.944.The correlation of the SAI total score and three subscales with insight of PANSS were-0.635,-0.305,-0.631 and-0.512 respectively (P ＜ 0.01).Conclusion The SAI has good reliability and validity.The scale is useful to measure insight in psychosis.

Objective: To explore the effect of galangin on the migration and invasion of breast cancer and its action mechanism. Methods: MTT assay was adopted to observe the effect of galangin at different concentration on the growth capacity of breast cancer cells MDA-MB-231. Wound healing and Transwell assay were conducted to detect the effect of galangin on the migration and invasion of MDA-MB-231 cells. Western blotting and immunofluorescence assay were adopted to investigate the migration and invasion related proteins including PI3K, AKT, MMP-2, and MMP-9. The effect of galangin on NF-κb p65 phosphorylation and NF-κb p65 nuclear transition in vitro was assayed by Western blotting and immunofluorescence assay. Furthermore, the antitumor effect of galangin in MDA-MB-231 tumor bearing BALB/c mice model was assayed in vivo. Results: Galangin inhibited the migration of MDAMB-231 cells in a dose-dependent manner. In wound healing and transwell experiments, with the increase in the concentration of galangin, the number of migrant MDA-MB-231 cells and the invasion capacity of breast cancer cells decreased. Galangin could decrease the protein expression of PI3K, Akt, MMP-2, and MMP-9. Besides, galangin significantly inhibit the phosphorylation of transcription factor NF-κB p65 and NF-κB p65 nuclear transition. Galangin also inhibited the growth of MDA-MB-231 cancer cells in vivo and down-regulated PI3K, Akt, MMP-2, and MMP-9. Conclusion: Galangin could inhibit the growth capacity of breast cancer cells MDA-MB-231 and block the migration and invasion of MDA-MB-231 cells. The mechanism for antitumor effect of galangin is through inhibiting NF-κb p65 activity and MMP-2/9 expression, and blocking PI3K-AKT signaling pathway.

AIM: To study the quality standard on catechu and its extract. METHODS: HPLC was used to determine the contents of (+) Catechin and (-) Epicatechin in catechu and its extract; UV spectrophotometry was applied to determine the contents of active tannic acid in catechu and its extract. RESULT: The contents of catechin and epicatechin are 19.4 and 4.36g/100g in catechu, respectively, and catechin and epicatechin are 48.39 and 3.94g/100g in its extract respectively. CONCLUSION: The method is accurate and reproducible, providing the basis for quality control of catechu and its extract.