Chlamydia pneumoniae is one of the most common pathogens causing community acquired pneumonia.It may activate immune cells,release mediators of inflammation and cytokine and cause immune response.Researches on its signal transduction after infection through Toll-like receptors may help prevent and cure chlamydia penumoniae infection disease.

From 1952 to 1990, 21 cases of malignant tumor of the appendix, including 14 cases of carcinoid tumor of appendix, 4 cases of appendix adenocarcinoma, 2 cases of appendix lymposarcoma and 1 case of appendix cystocarcinoma, were treated in the Affiliated Hospital of Wannan Medical College Among these 21 patients, 16 underwent appendectomy alone, 5 underwent appendectomy first and a right hemicolectomy was performed on later All of the 21 patients were followed up for 5～10 years,the survival rate was 90 5% and 90 5% respectively So if a malignant tumor of the appendix was suspected, a biopsy during operation should be done to determine the pathological type If the diagnosis was clear, the choice of operation mode must be made according to the size and the pathological type of the tumor In selective patient, a right hemicolectomy must be performed on

0.05). Conclusion Aspirin-vitamin C dispersible tablets is effective and safe for treating patients with URI.

Objective: To investigate the role of Toll-like receptor 4(TLR4) in the inflammatory reaction in the mouse model of Chlamydia pneumoniae(Cpn) infection.Methods: We divided 60 TLR4 mutant C3H/HeJ and 60 wild type C3H/HeN male mice into 4 groups: a mutant control(M),a mutant infection(M+I),a wild type control(W) and a wild type infection(W+I) group,each randomly assigned to 5 scheduled time points(1 d,4 d,7 d,14 d and 21 d).The animals in the M and W groups were intranasally inoculated with 2-sucrose phosphate(2SP) buffer,while those in the M+I and W+I groups with Cpn.All the mice were killed and their lung tissues were sampled on the above time points.The levels of TNF-? and IL-10 in the lung tissues were detected by enzyme-linked immunosobent assay(ELISA),histopathological examinations were performed,and the wet / dry weight(W / D) ratio was determined.Results: After Cpn inoculation,the lung tissues were characterized by patchy interstitial pneumonitis,predominantly infiltrated with neutrophil leukocytes in the earlier(7 d) stage and with lymphocytes in the later(14 d) stage.In the M+I group,the levels of TNF-? were elevated at 1 d([0.78?0.06] pg/mg),peaked at 4 d([1.22 ? 0.12] pg/mg) and then began to decrease,but was still higher than normal at 21 d;they were(1.10 ? 0.12,0.72 ? 0.12 and 0.52 ? 0.10) pg/mg at 7,14 and 21 d,significantly lower than in the W+I group([1.30 ? 0.16],[1.01 ? 0.19] and [0.71 ? 0.08] pg/mg,P

Objective To determine the effect of tumor necrosis factor ?(TNF ?) on p38 mitogen activated protein kinase (MAPK) activation in human pulmonary microvascular endothelial cells (HPMEC) in vitro Methods HPMEC was isolated and cultured in vitro Expression and activation of p38 MAPK were detected by western blot and immune complex kinase assay Results TNF ? induced phosphorylation and activation of p38 MAPK in HPMEC in a time/dose dependent manner which was suppressed by SB203580,a specific inhibitor of p38 MAPK Conclusion TNF ? plays a important role in activation of p38 MAPK and signal transduction in HPMEC

Objectives: To evaluate the efficacy, toxicity and side effects of CAP (cytoxan, adriamycin, cisplatin), NP(navelbine, cisplatin), GP(gemcitabine, cisplatin) in the treatment of patients with non small cell Lung cancer(NSCLC). Methods:146 patients with NSCLC diagnosed by pathology or cytology were treated with CAP, GP or GP regimen. Results:The overall response rate of the three groups (CAP, NP, GP)were 33.33%, 46.43% and 47.92% respectively. The major toxicity and side effects was bone marrow inhibition and the gastrointestinal reaction. Grade Ⅲ-Ⅳ side effects of CAP group were significant higher than the others. Conclusions:The regimen of navelbine and cisplatin or gemcitabine and cisplatin was more effective and less toxic than cytoxan, adriamycin, cisplatin and cisplatin.

Objectives:To examine whether lipopolysaccharide (LPS) induced apoptosis correlates with TNF ? release by type Ⅱ alveolar epithelial cells (AEC Ⅱ), whether TNF ? knockout (TNF KO) abrogates the induction of apoptosis by LPS and whether TNF ? is sufficient to induce apoptosis in this cell type. Methods:AEC Ⅱ was isolated from wild type mice and TNF KO mice. Cells were stimulated with LPS or recombinant murine TNF ? for 24 h. TNF ? in culture supernatant was determined by ELISA following LPS stimulation. Apoptosis was determined by the TUNEL assay after treatment with either LPS or TNF ?. Results:LPS induced apoptosis in wild type AEC Ⅱ in a concentration dependent manner. LPS induced AEC Ⅱ apoptosis was accompanied by a 11 fold increase from (0.073?0.065) ng/ml in controls to( 0.94?0.14)ng/ml in 50 ?g/ml of LPS( P

Objectives: To analyze the strategies of the diagnosis and treatment in patients with the pulmonary infection after renal transplantation. Methods: A retrospective analysis was made on 28 cases of pulmonary infections among 285 patients undergoing renal transplantation. Results: The morbidity of pulmonary infection was 9.82%(28/285). The majority pathogens of pulmonary infection in this study were pseudomonas, staphylococcus epidermidis, canadida and cytomegalovirus. Chest X ray was important in the diagnosis, but there was no specificity to determine the pathogens. The mortality in 28 pulmonary infection patients was 32.1%(9/28). Most of the patients suffered from pulmonary infection within 4 months after the operation, and the postoperative period from 2 months to 4 months was a high risk time window for death caused by pulmonary infections. Conclusions: The morbidity and mortality of pulmonary infections, especially severe pneumonia, in patients with renal transplantation, are higher than healthy people. Treatment following clinical epidemiology and strategy of microbiology play a very important role to reduce the mortality in those patients.

Objective To investigate the change trend of liver function indicators in different types of hyperlipidemia patients. Methods From July 2014 to July 2015 ,a total of 2571 patients with hyperlipidemia and 1063 normal people(control group) were collected in this research.All patients with hyperlipidemia were divided into the hypercholesterolemia group (high TC group ,n=258) ,hypertriglyceridemia group (high TG group ,n=859) and mixed type hyperlipidemia group(n=282) ,low high-density lipo-protein cholesterol group(low HDL-c group ,n=1172).The liver function indicators of total protein (TP) ,albumin (ALB) ,alanine aminotransferase(ALT ) ,aspartate aminotransferase (AST ) ,AST/ALT and globulin (GLB ) were measured in each group. Results The TP and ALB levels in the high TC group ,mixed group and low HDL group were significantly decreased compared with the control group ,the difference was statistically significant (P<0.05).The ALT ,AST ,AST/ALT and GLB levels in the four hyperlipidemia groups had different degrees of increase as compared with the control group ,the difference was statistically signifi-cant(P<0.05).The TP and ALB levels in the mixed group and the low HDL group were significantly lower than those in the high TG group and high TC group ,the difference was statistically significant (P<0.05).The ALT level in the low HDL group was sig-nificantly higher than that in the other three groups ,the difference was statistically significant (P<0.05).The AST/ALT level in the mixed group was significantly higher than the other three groups (P<0.05).The GLB level in the high TC group was signifi-cantly higher than the other 3 groups with statistical difference (P<0.05).Conclusion Blood lipid increase may induce the liver function injury.The detection of liver function indicators in the patients with hyperlipidemia will contribute to the early detection and treatment of fatty liver.

Objective To assess the technical feasibility and efficacy of the combined application of a flexible,self-expanding neurovascular stent(Neuroform)and Gugliebni detachable coils(GDC)in the management of wide-necked intracranial aneurysms in humans.Methods Sixty-five wide-necked aneurysms which underwent 65 endovascular procedures were performed by using intracranial stent and GDC.There was a total of 30 aneurysms at basilar artery including 16 at the basilar tip,9 at the basilar trunk and 5 at the beginning of the basilar artery.And there were 30 aneurysms located at the posterior communicating artery, and 5 aneurysms located at the vertebral artery.The Neuroform stents were deployed to cover the neck of aneurysms.Another microcatheter was introduced into the aneurysm sac through the stent interstices and then detachable coils were released to embolize the aneurysms.Results The combined procedures were successful in all of the 65 patients with wide-necked aneurysms.The stent could pass smoothly through the intracranial artery and got released.Complete occlusion was achieved in 60 patients and incomplete occlusion in 5 patients.In-stent thrombosis occurred in 2 patients.All patients recovered well.Forty-two patients had followe-up angiography at 3 to 6 months after the procedure.Among them,no filling was found for the 39 aneurysms which were densely packed,and 3 aneurysms had neck remnant.Conclusion The implantation of Neuroform stent as a complimentary device to GDC coiling is easy and safe for embolization of wide-necked intracranial aneurysms.It has great advantage for treatment of wide-necked intracranial aneurysms.