Attention Deficit Disorder with Hyperactivity ; epidemiology ; Child ; Comorbidity ; Humans

It is the requirement to change the utility reading of medical undergraduates and to realize the functions of medical academic library for medical academic library to popularize reading service .The serial reading populari-zation service practice was thus described in this paper with certain feasible suggestions put forward for the further reading popularization activities.

Attention Deficit Disorder with Hyperactivity ; diagnosis ; therapy ; Humans

Objective: To establish the quality standard for Ganmaoyangkeling Tablets(Radix Isatidis, Herba Solidaginis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, etc.). Methods: Radix Glycyrrhizae and Herba Solidaginis were identified by TLC, and the content of quercetinum was determined by HPLC. Results: Quercetinum shows a good linear relationship in the range of 0.05～0.40?g ( r =0.99997), and the average recovery is 101.9%, RSD is 2.07%. Conclusion: These methods is simple, accurate and specific and can be used for the quality control of Ganmaoyangkeling Tablets.

By using electron microscopy histochemical method, light microscopy histochemical method and microspectrophoto meter, we studied the ACP activity of the principal cell of epididymal duct in the postnatal 10-90 days rats. The results revealed that ACP activity increased with the increase days. We also observed the following phenomena: in the postnatal 10 days rats the ACP activity was only localized on GERL and vesicles around GERL; by in the postnatal 19 days rats, the ACP activity was localized on lysosome; and 35 days later, the ACP was also localized on the saccules on the forming face of Golgi complex. The significance of the above changes in ACP activity of the principal cell of epididymal duct in postnatal rats was discussed in this paper.

Objective To measure the serum levels of angiopoietin-like protein (ANGPTL) 3, ANGPTL4 and RANKL in patients with rheumatoid arthritis (RA), and the relationship between ANGPTL3/ANGPTL4 and clinical manifestations were analyzed. Methods Blood samples were drawn from 72 patients with RA and 28 healthy subjects. Serum levels of ANGPTL3, ANGPTL4 and RANKL were tested by enzyme linked immuno-sorbent assay (ELISA). Nonparametric rank sum test was used for the comparisons between groups and Spearmanˊs correlation test was used for correlation analysis. Results ① The serum level of ANGPTL3 was significantly increased in RA group compared to healthy group [800.325(577.477, 1 750.636) pg/ml vs 487.900 (382.340, 565.499) pg/ml, P<0.05, Z=-6.082]. ② The serum level of ANGPTL4 was significantly increased in RA group compared to healthy group [1 036.199(853.347, 1 746.677) pg/ml vs 706.095(558.571, 807.302) pg/ml, Z=-5.962, P<0.05].③ The serum levels of ANGPTL3 and ANGPTL4 in RA patients were increased with the rise of disease activity (DAS 28) [ANGPTL3 in the low disease activity groupwas 457.265 (373.709, 605.296) pg/ml, that of the moderate disease activity group was 785.815(679.156, 1 308.785) pg/ml, that of the high disease activity group was 1 502.038 (817.713, 1 960.493) pg/ml, P<0.05; ANGPTL4 in the low disease activity group was 737.604 (467.040, 918.222) pg/ml, that of the moderate disease activity group was 991.227 (819.456, 1 699.972) pg/ml, and that of the high disease activity group was 1 842.310 (1 252.023, 2 669.902) pg/ml, P<0.05].④ANGPTL3 and ANGPTL4 was positively correlated with RANKL (r=0.554, 0.619, P<0.01).⑤ The serum level of ANGPTL4 was significantly increased in patients with bone destruction, compared to those without bone destruction [1 624.071(949.432, 2 622.371) pg/ml vs 927.590(737.604, 1 409.798) pg/ml, Z=-2.483, P<0.05]. Conclusion The serum levels of ANGPTL3 and ANGPTL4 are increased in RA, and are both positively correlated with disease activity, moreover, ANGPTL4 is associated with pathological bone destruction.

This study revealed that the content of intra and extracellular polysaccharides may be related to the cultural conditions.With decreasing NaHCO 3 or NaNO 3 concentration,the content of intracellular polysaccharide was reduced,Reduction of NaNO 3 concentration still caused the rise of extracellular polysaccharides.The K 2HPO 4 or NaCl concentration also had an effect on the production of both intracellular and extracellular polysaccharides.When PH value of media maintained at 8.0,there was the highest content of intra and extracellular polysaccharides.The same high content also occurred after culture of nine days.In addition the content of intracellular polysaccharide was higher under continuous light than under the light/dark circle.

Objective To investigate the effect of mycophenolic acid (MPA) on the endotheline-1 (ET-1) induced proliferation, contraction and migration of pulmonary arterial smooth muscle cells (PASMCs)and to explore the mechanism of MPA on pulmonary arterial hypertension (PAH), and the effect of exogenous guanosine nucleotide reversing anti-proliferative effect of MPA. Paired-samples t-test was used for statistical analysis. Methods MTT test, scarification test, Millicell cell culture insertion and the length of PASMCs mcasured under microscope were used. Results The A values of group ET-1 + low concentration MPA decreased when compared with group ET-1 (0.348±0.036 vs 0.447±0.013, t=6.357, P=0.000) and the A values of group ET-1 + high concentration MPA was further decreased. The A values of group ET-1 + low concentration MPA + guanosine was higher than that of group ET-1 + low concentration MPA (0.390±0.018 vs 0.348 ±0.036, t=2.573, P=0.028). The average migration distance and the average migration numbers of PASMCs of groups MPA was decreased than that of group ET-1. The average cell length of PASMCs of groups MPA was increas ed than that of group ET-1. Conclusion MPA can effectively inhibit the proliferation,contraction and migration of PASMCs by ET-1 induction. The IMPDH may play a role in anti-proliferative effect of MPA on PASMCs, but is unnecessary to be the sole mechanism. These findings has provide new insight into the mechanisms of mycophenolate mofetil in the treatment of PAH.

As a species of acute respiratory tract pathogen in mankind, Chlamydia pneumoniae can cause upper respiratory tract infection, pneumonia, it may also play an important role in bronchial asthma, chronic obstructive pulmonary diseases, sarcoid, lung cancer.

ObjectiveTo observe the effect of mycophenolate mofetil(MMF) on systolic pulmonary arterial pressure (SPAP), right ventricle index (RVI), cytokines, receptors of pulmonary artery hypertension (PAH) rat models. MethodsThe rat models of monocrotaline (MCT)-PAH were developed. Thinty-two Sprague-Dawley male rats were divided randomly into four groups: the control group, the MCT, the MCT +low-dose MMF (20 mg·kg-1·d-1, MMF 20) and the MCT + high-dose MMF(40 mg·kg-1·d-1, MMF 40).There were eight rats in each group. SPAP, RVI, cytokines and receptor in the serum and lung tissue were measured in all rats. Comparisons between multiple groups were performed with q test and rank sum test.Results① Low-dose and high-dose MMF group were compared with the MCT group, SPAP[(40±13),(53±10) vs (68±10) mm Hg]and RVI(0.36±0.06, 0.38±0.03 vs 0.44±0.05) were reduced and showed statistically significant difference respectively(P＜0.05). (②) Compared with the MCT group, basic fibroblast growth factor(bFGF) of serum in both low-dose and high-dose MMF group were reduced and showed statistical difference respectively[(2.3±1.9), (2.7:t±1.3) vs (6.9±5.4) pg/ml, P＜0.05]. ③ Low-dose and high-dose MMF group were compared with the MCT group and showed that MA was decreased and the musculization of arterial wall was improved. ConclusionMMF can reduce SASP, RVI, number of MA. The inhibitory effect of MMF in PAH is possibly by inhibiting cytokines and receptors.