Adolescent ; Adult ; Age Determination by Skeleton ; methods ; Child ; Child, Preschool ; China ; Diagnosis, Computer-Assisted ; methods ; Female ; Humans ; Male

Adult ; Female ; Humans ; Intestinal Obstruction ; etiology ; therapy ; Postoperative Complications ; etiology ; therapy ; Rectal Neoplasms ; radiotherapy ; surgery

FOXM1 is a member of the FOX family of transcription factors and has been confirmed to participate in cell prolifera-tion and cell cycle progress. FOXM1 has been reported to play an important role in more than 20 types of human neoplasms, and its up-regulation promotes tumor cell proliferation, invasion, metastasis, chemotherapy drug resistance, and resistance to apoptosis. In recent years, various studies have shown that FOXM1 also plays an important role in tumor metabolism. The metabolism of cancer cells deter-mines the growth and survival of cancer, and metabolic alteration is a key feature of tumor cells. The connection between metabolic ab-normalities and tumorigenesis has been reported. The O-linkedβ-N-acetylglucosamine transferase regulates the expression of FOXM1, which helps tumor cells survive without damage caused by oxidative stress. This review aims to summarize the known relationships of FOXM1 and tumor cell metabolism, as well as its role in the potential pathways of tumor metabolism.

The application of human embryonic stem cell(hES cell) contributes a lot to the disease treatment,and shows a great potentiality especially in organ transplantation and tissue engineering.Although hES cell therapy has not reached clinical application,some newly achieved results are encouraging.This review mainly presents results recently published in applying hES cell for disease treatment.

Objective To study the efficacy, safety and compliance of insulin human analog insulin aspart (NovoRapid) and insulin zinc protamine analog (Basulin) in the application on type 2 diabetes mellitus(T2DM)pafients during perioperative period. Methods Sixty T2DM patients were divided into 3 groups (n=20). Group A was treated with NovoRapid+Basulin, group B was treated with insulin human (Humulin R + Humulin N) and group C was treated with insulin pump (Humulin and NovoRapid). Blood glucose was detected in all of the patients before the meal, 2 hours after meal, before the sleep and during the operation. Results Blood glucose could all achieve the control level with the three methods of treatment. The rates of hypoglycemia in the three groups were 10%, 30% and 5% respectively, and the rates of patient satisfaction were 95%, 70% and 100% respectively. Conclusions Insulin human analog could control blood glucose effectively and safely, the compliance is also high. It is feasible of applying insulin human analog in the blood glucose control during perioperative period.

Adolescent ; Humans ; Male ; Werner Syndrome

OBJECTIVE: To develop a sensitive and specific reverse-phase high-performance liquid chromatography (RP-HPLC) method to determine propofol in follicular fluid. METHODS: Thymol was added into follicular fluid samples as the internal standard, and the samples were then precipitated with methanol. After centrifugation at 20000 r·min-1 for 15 min, 20 μL of supernatant was directly injected into an Agilent Extend-C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was composed of methanol-water (77:23). Analysis was run at a flow rate of 1.0 mL ·min-1. Fluorescence detection was applied with excitation and emission wavelengths of 276 and 310 nm, respectively. RESULTS: The calibration curves had good linearity in the range of 0.108-5.4 mg·L-1. The linear regression equation was A=0.2074ρ-0.0012(r=0.9993). The limits of detection and quantity proved to be 54 and 108 μg·L-1, respectively. The method recoveries for low, intermediate and high concentrations were 117.9%, 101.7% and 99.4%, respectively. The RSDs of inter-day assay were 4.10%, 1.28% and 0.20%(n=5), and intra-day assay were 4.34%, 2.33% and 0.61%(n=5), respectively. CONCLUSION: The method is accurate and convenient. It can be used to determine propofol for studying its clinical pharmacokinetics.

Objective To evaluate the influence of Fuzheng Huayu decoction on different pathological stages fibrotic liver tissue and activated hepatic stellate cells (HSCs). Methods Use a carbon tetrachloride (CCl4)-induced liver cirrhosis rat model system. The drug intervention was administered via oral-gastric irrigation in building, the end of week 2 and 4. Rats were batch sacrificed at the end of week 2 , 4, and 6. Liver fibrosis was evaluated by histology, and expression of a-smooth muscle actin (α-SMA) was determined by immunohistochemistry. Between-group comparisons were made by completely random design and ANOVA with Bonferroni correction. Results At the end of weeks 2, 4 and 6, the model group and drug intervention groups had significantly higher area ratio of liver fibrosis and higher expression of α-SMA than the normal group, but the three drug intervention groups had significantly less area ratio of liver fibrosis and less expression of α-SMA than the model group and the drug intervention 1 group showed the most robust decrease (P<0.05). Conclusion The Fuzheng Huayu decoction leads to down-regulated α-SMA expression and reduced degree of liver fibrosis on different pathological stages of fibrotic liver tissue, and the earlier drug intervention, the better the results.

Objective To evaluate efficacy and therapeutic satisfaction of novel analogue glargine combined with repaglinide in type 2 diabetic patients(T2DM) after switching from the most frequently used twice daily injection of premixed insulin(Humulin 70/30 30% rapid acting and 70% NPH before meals).Methods 18 patients with T2DM whose blood glucose control was poor receiving twice daily premixed insulin had been switched to receive a once daily injection of glargine together with repaglinide and biguanides(metfomin) or TZDS(reglitazone) was continued if it was taken to relieve insulin resistance.After 16 weeks the blood glucose concentrations and HbA1c levels were detected and hypoglycemia was reported.Results At the time of post-16week after the switch,fasting blood glucose access to normal;blood glucose fluctuation relieved and HbA1c levels decreased(from 7.84?0.21 to 6.52?0.13).Symptomatic hypoglycemia decreased.Conclusion These results indicate that insulin glargine combined with repaglinide can be replaced from the twice daily premixed insulin injection regiment for the T2DM even with poor glucose control.