Objective To approach the toxicity of Danggui Sini plus Wuzhuyu Shengjiang Tang and Asarum heterotropoides Fr.var. mandshuricum Kitag. Method To observe the Ca2+, Na+-K+-ATPase from the mice renalis tissue in order to prove the medicine curative effect. Result The Danggui Sini plus Wuzhuyu Shengjiang Tang and Asarum heterotropoides Fr.var. mandshuricum Kitag. can obviously increase the Ca2+ and decrease the Na+-K+-ATPase of the mice. Conclusion The Danggui Sini plus Wuzhuyu Shengjiang Tang and Asarum heterotropoides Fr.var. mandshuricum Kitag. has very obviously toxicity to mice by influence the Ca2+ and Na+-K+-ATPase of the tissue homogenate.

Objective To investigate the protein and gene expression of bcl-2, bcl-6 in diffuse large B-cell lymphoma (DLBCL). Methods 73 cases of DLBCL were selected for study using the Envision immunohistochemistry method with a panel of antibodies CD3, CD10, CD20, bcl-6, bcl-2, MUM-1. The bcl-2gene expression in 57 of 73 cases with chromosome translocation t (14; 18), breakage and amplification of 3q27 chromosome in 54 of 73 cases were detected by fluorescence in situ hybridization (FISH) method. Results The percentages of tumor cells expressing CD10, bcl-6, MUM-1, bcl-2 were separately 15.1%, 38.4 %, 71.2 %, 79.2 %. t (14;18) chromosomes were detected in 16 of 57 cases (28.1%). The expression of bcl-2 protein have significantly correlated with immunophenotype subtype (P=0.035), and t (14;18) was significantly correlated with the prognosis (P=0.045). There were no association between the expression of bcl-2 protein and t (14;18)(P=0.710). 11 of 54 cases were presented with 3q27 chromosomal breakage (20.4 %), and 14 cases were chromosomal amplification (25.9 %). The prognosis of cases with positive bcl-6 protein was better than that with negative protein obviously. There was no relationship between bcl-6 and 3q27 chromosomal breakage or amplification (P=1.000). Conclusion The expression of bcl-2, bcl-6 protein and gene were different events and had the different significance on DLBCL. The expression of bcl-2 protein was a prognostic marker correlated with immunophenotype subtype, and GCB type with the positive expression of bcl-2 protein had the poor prognosis. Conversely, t(14;18) was an independent event for the prognosis, and the positive expression have the poor prognosis. Patients who require the target therapy should be detected for the t(14;18). The expression of bcl-6 protein was beneficial to the judgment of DLBCL prognosis, it could be an independent factor of the prognosis. 3q27 chromosomal breakage may be a hint to the poor prognosis.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Melanosis ; drug therapy ; therapy ; Middle Aged ; Young Adult

OBJECTIVEThis study aims to construct a three-dimensional finite element model of a maxillary anterior teeth retraction force system in light wire technique and to investigate the difference of hydrostatic pressure and initial displacement of upper anterior teeth under different torque values of tip back bend.

METHODSA geometric three-dimensional model of the maxillary bone, including all the upper teeth, was achieved via CT scan. To construct the force model system, lingual brackets and wire were constructed by using the Solidworks. Brackets software, and wire were assembled to the teeth. ANASYS was used to calculate the hydrostatic pressure and the initial displacement of maxillary anterior teeth under different tip-back bend moments of 15, 30, 45, 60, and 75 Nmm when the class II elastic force was 0.556 N.

RESULTSHydrostatic pressure was concentrated in the root apices and cervical margin of upper anterior teeth. Distal tipping and relative intrusive displacement were observed. The hydrostatic pressure and initial displacement of upper canine were greater than in the central and lateral incisors. This hydrostatic pressure and initial intrusive displacement increased with an increase in tip-back bend moment.

CONCLUSIONLingual retraction force system of maxillary anterior teeth in light wire technique can be applied safely and controllably. The type and quantity of teeth movement can be controlled by the alteration of tip-back bend moment.

This article made a summarization and analysis on acupuncture related empirical and clinical research literature in Science Citation Index (SCI) periodicals in 2009.Authors used acupuncture respectively as the key word to retrieve in SCI periodicals in 2009 through Web of Science and got 630 articles.The articles were summarized and analyzed on experimental design, experimental research content, disease category, acupuncture accident and systematic review of clinical effect from native and abroad.

Objective To investigate the distribution and antimicrobial resistance of pathogens from lower respira-tory tract in patients in intensive care unit (ICU),so as to provide reference for clinical treatment.Methods Distri-bution and antimicrobial susceptibility of pathogens isolated from ICU patients’sputum obtained through fiberbron-choscope between 2011 and 2014 were analyzed retrospectively.Results A total of 3 454 pathogenic strains were isolated between January 1 ,2011 and December 31 ,2014,the percentage of gram-negative bacteria,gram-positive bacteria,and fungi were 84.11 %,14.50%,and 1 .39% respectively.The detection rates of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella spp .in 2011 -2014 were 38.46% -73.33% and 26.95% -37.06% respectively. Enterobacteriaceae strains had low resistance rates to imipenem and meropenem (<20.00%);resistance of Acinetobacter baumannii was higher than Pseudomonas aeruginosa ,both had low resistance rates to amikacin(3.32%-37.16%);vancomycin-and linezolid-resistant strains were not found among Staphylo-coccus .In 2011 - 2014,detection rates of methicillin-resistant Staphylococcus aureus (MRSA)were 42.86% -61 .22%,methicillin-resistant coagulase-negative staphylococcus (MRCNS)were 86.96% - 91 .67%;resistance rates of Enterococcus faecium was higher than Enterococcus faecalis ,vancomycin-resistant strains were not found among Enterococcus faecalis and Enterococcus faecium ;the major fungus was Candida albicans .Conclusion Anti-microbial resistance of pathogens isolated from lower respiratory tract is getting more serious,clinicians should pay attention to non-antimicrobial infection control strategies in addition to rational use of antimicrobial agents.

Adolescent ; Adult ; Altitude ; Exercise ; Free Radicals ; metabolism ; Humans ; Male ; Placebos ; Tyrosine ; pharmacology ; Young Adult

The ingredients of five kinds of Zhejiang's yellow Chrysanthemum morifolium with different flower blossoming stages were comparatively analyzed. Polysaccharides, total flavonoids, volatile oil, alcohol extract, water extract, chlorogenic acid, luteolin, 3,5-O-dicaffeoyl quinic acid and fingerprint of the ingredient were determined as indicators. During flower blossoming stages, the ingredients of Ch. morifolium showed a big difference with a certain variation. At the early opening stage, the contents of flavonoids and volatile oil were higher, the content of chlorogenic acid, luteolin, 3,5-O-dicaffeoyl quinic acid were higher in the middle of the flowers 50% -80% fowers blossoming degree is the optimal time for harvest.
China ; Chlorogenic Acid ; analysis ; Chromatography, High Pressure Liquid ; Chrysanthemum ; chemistry ; growth & development ; Drugs, Chinese Herbal ; analysis ; Flavonoids ; analysis ; Flowers ; chemistry ; growth & development ; Quality Control ; Quinic Acid ; analysis

AIM:To find the sensitive targets by observing the changes of early fundus detection in function and structure and correlation of systemic monitoring indicators on non-proliferative diabetic retinopathy.

METHODS: Totally 20 normal peoples(40 eyes)in normal control group and 105 in-patients(210 eyes)in diabetic group, which was divided into two groups, 24 cases(48 eyes)in no diabetic retinopathy(NDR)group, 81 cases(162 eyes)in non-proliferative diabetic retinopathy(NPDR)group. The NPDR group was divided into three groups: mild NPDR group 30 cases(60 eyes), moderate NPDR group 26 cases(52 eyes)and severe NPDR group 25 cases(50 eyes). The retinal blood flow(RBF)of Heidelberg retinal flowmeter(HRF), the OS2 amplitude of oscillatory potentials(OPs)and the subfoveal choroidal thickness(SFCT)of OCT were measured in normal control group and diabetic group. The duration of diabetes, blood pressure, fasting blood glucose, hemoglobin, blood lipids and coagulation were also examined in each group. All test results in each group were compared and analyzed statistically.

RESULTS: HRF: the value of RBF in NDR and NPDR group was significantly lower than that in the normal control group(P<0.05); the value of RBF in moderate NPDR group was obviously higher than that in the other groups(P<0.05); the value of RBF in the temporal side of the optic disc was higher than that in the nasal side of the optic disc in NPDR group(P<0.05). OPs: the amplitude of OS2 in NDR and NPDR group was significantly lower than that in the normal control group(P<0.05); the amplitude of OS2 of the severe NPDR group and moderate NPDR group were significantly lower than that in mild NPDR group and NDR group(P<0.05). OCT: with the progression of DR, the value of SFCT were thinner and thinner(P<0.05). The duration of diabetes without therapy, diastolic blood pressure, fasting blood glucose, hemoglobin, cholesterol, low density lipoprotein, fibrinogen, D-dimer positively positive correlated with degree of DR(r_s=0.722, 0.791, 0.864, 0.473, 0.611, 0.735, 0.591, 0.554, P<0.05). The occurrence of DR was consistent with other microvascular complications.

CONCLUSION:The detection of HRF, OPs and OCT can be used as sensitive indicators for the early diagnosis of DR. They can earlier and more accurately reflect the changes of microcirculation function and structure of the fundus. The duration of diabetes, blood pressure, fasting blood glucose, hemoglobin, blood lipids, coagulation status are positive correlated in DR progression. The monitoring of these indicators and disseminating timely healthy information own important clinical value in primary medical care.