Objective: To observe the effect of GETO on learning and memory of experiment AD model rats and search its cholinergic mechanism of action.Methods: ?-amyloid protein(A?_(1-42)) injection into hippocampus in rat was applied to make experimental rat model of AD.Morris water maze test were employed to investigate the function of learning and memory in AD model rats after 4 weeks,and immunohistochemical stain was done with Choline Acetyltransferase(ChAT) and nerve growth factor(NGF) antibodies after Morris water maze test,and system of image pro-plus were used to gain the number and grey degree of positive neurons.Results: In Morris water maze test: The mean escape latent period of the Model rats obviously prolonged than that of the Normal rats,the number of traversing flat roof obviously decreased.The mean escape latent period and the number of traversing flat roof of the GETO rats significantly shorten than the model rats.Immunohistochemical test: The number of positive staining neuron in hippocampus CA1 of ChAT and NGF in the GETO rats was not significantly different from those in the Normal rats and the Donepezil rats,but significantly different from those in Model rats with injection of A?_(1-42)(P

AIM: To determine the contents of baicalin, berberine, rhein in Yiqing Capsule(Radix et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis) by HPLC. METHODS: The HPLC system consisted of Kromasil C 18 column (150 mm?4.6 mm, 5 ?m), 0.02 mol?L -1 phosphoric acid (TEA adjusted to pH=6.82)-acetonitrile(40∶11) mixture as mobile phase, detection wavelength at 254 nm, flow rate of 1.0 mL?min -1. RESULTS: The calibration curves of three ingredients were linear within the range of 3.666-73.32 mg?L -1(r=0.9998, baicalin), 0.3112-15.56 mg?L -1(r=0.9998, rhein), 1.221-50.04 mg?L -1(r=0.9999,berberine), respectively. The average recovery of baicalin is 102.3%(RSD=2.5%), berberine for 101.8%(RSD=1.8%), and rhein for 98.3%(RSD=2.9%). CONCLUSIONS: The method is rapid, sensitive and accurate.

Objective To explore the pathogenic mechanism associated with insulin resistance(IR) and adiponetin(APN) abnormality in carotid atherosclerosis(CAS) patients complicated with nonalcoholic fatty liver disease(NAFLD),and to study their relationships with traditional Chinese medical(TCM) syndrome types.Methods Seventy-four CAS patients complicated with NAFLD(group A) and 25 CAS patients(group B) were enrolled into the study.Fasting blood glucose(FBG),fasting serum insulin(FI),and serum APN levels were measured.IR and APN were evaluated by the homeostasis assessment(HOMA) method.HOMA-IR and APN in group A were compared with those in group B,and bivariate correlation analysis was made between the two indexes in group A.The relationship of HOMA-IR and APN with TCM syndrome types was analyzed in group A.Results (1) HOMA-IR was obviously increased,and APN was obviously decreased in group A(P

Pseudomonas aeruginosa,a ubiquitous organism,shows a remarkable resistance to antibiotics because of its low permeability of the outer-membrane,over-expression of efflux pumps,inactivation of antibotics,or mutations in targets.These mechanisms are often present simultaneously,and increase the burden on clinical therapy.

Objective To compare the clinical effects of modified single open-door laminoplasty combined with segmental lateral screw fixation with conventional single open-door combined with continuous screw fixation in the treatment of multilevel cervical spondylotic myelopathy (MCSM).Methods Retrospective analysis was used to analyze the fifty-five cases of MCSM who underwent posterior operation in Lianyungang Hospital Affiliated to Xuzhou Medical University from March 2009 to January 2017.Among them,26 cases in the modified group were treated by modified single open-door laminoplasty combined with segmental lateral screw fixation;29 cases in the traditional group were treated by conventional single open-door combined with continuous screw fixation.JOA score collected before the operation and at the last time of the follow-up,the cervical spine mobility,cervical curvature,cervical axial symptom,C5 nerve root palsy and the incidence rate of screw fracture in both groups were compared.Operation time and the intraoperative blood loss were recorded.Results 55 cases were all followed up.The cervical spine mobility at the last time of the follow-up in the modified group was significantly higher than that of the traditional group ((39.63°±5.98°) vs.(36.14°±6.05°),P=0.036).The cervical curvature in the modified group was higher than that of the traditional group ((19.83°±5.61°) vs.(16.02°±7.05°),P=0.030).The operation time and intraoperative blood loss in the modified group were significantly lower than those of the traditional group ((116.3±13.2) min vs.(128.7±14.3) min,(186.7±39.8) ml vs.(228.7±42.5) ml,P=0.002,0.000).In the modified group,the proportion of patients with postoperative cervical axial symptom was 15.38%,the rate of C5 nerve root palsy cases was 8.69%,compared with 48.27% and 34.48%,respectively in the control group,the difference between the two groups was statistically significant (P=0.011,0.022).In the traditional group,2 cases of screw fracture were found at the last follow-up,while there was no such case in the modified group.Conclusion The modified cervical single open-door laminoplasty combined with segmental lateral screw fixation can obtain good effect of nerve decompression,maintain cervical mobility and cervical curvature,reduce the occurrence of postoperative cervical axial symptom,C5 never root palsy,screw fracture and other complications,is a safe and effective surgical method.

BACKGROUND: Abnormal changes of bone mineral density (BMD) easily occur in patients with non-insulin-dependent diabetes mellitus (NIDDM),however the mechanisms are still unclear.OBJECTIVE: To investigate the pathophysiological features of changes of BMD and metabolism in patients with NIDDM from the angle of traditional Chinese medicine (TCM) syndrome types.DESIGN: A case controlled and comparative observation.SETTING: Department of Endocrinology of Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA PARTICIPANTS: Totally 103 cases (55 males and 48 females) of patients with NIDDM admitted in Department of Endocrine of Guangzhou General Hospital of Guangzhou Military Area Command of PLA from April 2003 to April 2004 were enrolled. They were, according to TCM syndrome types, divided into 3 groups: yin deficiency with heat excess (32 cases),deficiency of both qi and yin (36 cases), and deficiency of both yin and yang (35 cases) groups. The healthy persons of 30 staff, 16 males and 14 females, in the hospital were concurrently recruited as control group. Informed consent was obtained from all the subjects.METHODS: ①A dual-energy X-ray absorption meter was used to determine the bone mineral densities of orthophoric 1-4 lumber vertebrae, total hipbone and head of femur in left hip in each group. ② Radioimmunoassay was taken to measure the content of serum osteocacin (OC). ③Colorimetry was used to measure the level of serum tartrate resistant acid phosphatase (TRACP). ④ q-test and x2-test were respectively used for comparison of measurement and enumeration data.MAIN OUTCOME MEASURES: ①The BMD of the 1-4 lumber vertebrae, total hipbone and head of femur in left hip in each group. ②The morbidities of osteoporosis of subjects in each group. ③ The levels of OC and TRACP in each group.RESULTS:All 103 NIDDM patients and 30 healthy subjects entered the final result analysis. ① The BMD of the 1-4 lumber vertebrae, total hipbone and head of femur in left hip of subjects: The BMD in yin deftciency with heat excess, deficiency of both qi and yin and control groups were obviously higher than that in deficiency of both yin and yang group (P ＜ 0.05-0.01 ) ② The morbidity of osteoporosis: The morbidities of osteoporosis in yin deficiency with heat excess, deficiency of both qi and yin and control groups were obviously lower than that in deficiency of both yin and yang group[34%(ll/32),42% (15/36), 33% (10/30), 71% (25/35),P ＜ 0.01]. ③ The level of OC: The levels in yin deficiency with heat excess and deficiency of both qi and yin groups were obviously higher than that in deficiency of both yin and yang group (P ＜ 0.01 ), but lower than that in deficiency of both yin and yang group (P ＜ 0.05). ④ The level of TRACP: There was no significant difference among groups(P ＞ 0.05).CONCLUSION: ① The volume and formation of bone in NIDDM patients of deficiency of both yin and yang syndrome type were obviously lower than those in healthy persons and NIDDM patients of yin deficiency with heat excess and deficiency of both qi and yin syndrome types, and the osteoporosis morbidity in them was obviously higher than those in healthy persons and the other two syndrome-type patients. ② The quantity and formation of bone in NIDDM patients of deficiency of both yin and yang syndrome type were near to those in healthy persons.

Our study showed that the concentration of plasma ammonia in patients with hepatic encephalopathy was increased significantly than that in patients with decompensated liver cirrhosis, primary hepatocellular carcinoma and fulminant hepatitis. The concentration of serum free tryptophan and "free/total" tryptophan ratio in patients with hepatic encepha-lopathy were significantly increased as compared with patients of other three groups. The concentration of serum serotonin was not significantly increased and that of serum 5-hy- droxyindolacetic acid was significantly increased in comparison with normal controls. In addition, the concentrations of serum free tryptophan and serum "free/total" ratio were closely positive correlated with the degree of hepatic encephalopathy, the concentrations of. plasma ammonia and serum free tryptophan and serum "free/total" ratio were higher in patients with hepatic coma than that of patients regainning consciousness, but the difference was not statistically significant. The role of plasma ammonia and serum tryptophan in pathogenesis of hepatic encephalopathy was discussed

Objective To investigate the clinical significance of the expression of fragile histidine triad (FHIT) protein in primary hepatocellular carcinoma (HCC).Methods Immunohistochemical (S-P) method was used to detect the expression of FHIT in the 46 patients with HCC and 10 normal controls.Results In the patients with HCC,the expression rate of FHIT in the tumor tissue was 56.52%,significantly lowerthan that in adjacent non-tumor tissue and normal tissue.The absence of FHIT protein was correlated neither with the size,tumor capsule,serum AFP level nor with chronic hepatitis B virus infection and cirrhosis of liver.There was significant relationship between the expression of FHIT and differentiation and thrombus in the portal vein.Conclusion The loss of FHIT gene protein is a frequent event in HCC.Furthermore,the loss is closoly related to tumor prognosis prognosis and FHIT loss.

Objective To investigate the effect of Brocho-Vaxom in curing chronic pulmonary heart disease and the influence of patient's immunity.Methods 58 patients with chronic pulmonary heart disease were randomly divided into two equal groups.Each patient of treatment group took a Brocho-Vaxom capsule(7mg/cap) every.The respiratory tract infection times and duration were observed.The serum IgA,IgG,IgM level were tested by fast immunonephelometry.The sub-type lymphocyte was tested by SP,and the NK cell's activity was tested by MTT.Results The infection time obviously declined in treatment group which the average was (2.5?1.8) in five months as compared with that in the controlled group(P