1.The effects of JARID1B siRNA on proliferation and apoptosis in HL-60 cell.
Xu-dong MA ; Hui-dan HAN ; Yi-qun HUANG ; Yong ZOU
Chinese Journal of Hematology 2012;33(5):392-396
OBJECTIVETo study the effect of small interfering RNA(siRNA) targeting JARID1B gene on the proliferation and apoptosis in HL-60 acute promyelocytic leukemia cell line, and to explore its mechanisms.
METHODSThe JARID1B siRNA was transfected into HL-60 cells using Lipofectamine(TM) 2000(Lipo) vector. The proliferation inhibition by siRNA targeting JARID1B was detected by MTT, cells apoptosis by flow cytometry, the mRNA expression of JARID1B by RT-PCR, the protein expression of JARID1B, Bcl-2, procaspase-9, procaspase-3, c-myc and P27 and histone methylated H3K4 by Western blot.
RESULTSsiRNA targeting JARID1B upregulated histone methylated H3K4 level, inhibited the proliferation of HL-60 cells, and induced the cells apoptosis. After transfection of siRNA targeting JARID1B at 0, 30, 60, 120 nmmol/L for 24 hours, the apoptotic rate were (11.0 ± 3.6)%, (35.2 ± 5.1)%, (52.7 ± 3.8)%, and (62.0 ± 5.7)% respectively (F = 70.27, P < 0.01). The protein expression of P27 was upregulated, and Bcl-2, procaspase-9, procaspase-3, c-myc was down regulated.
CONCLUSIONSJARID1B siRNA upregulates histone methylated H3K4. It reduces HL-60 cells proliferation and apoptosis by up regulating the p27 expression and down regulating the Bcl-2, procaspase-9, procaspase-3, c-myc expression. It might be a new therapeutic targeting for human leukemia.
Apoptosis ; Caspase 3 ; metabolism ; Caspase 9 ; metabolism ; Cell Proliferation ; Cyclin-Dependent Kinase Inhibitor p27 ; metabolism ; Gene Expression Regulation, Leukemic ; Gene Targeting ; HL-60 Cells ; Histones ; metabolism ; Humans ; Jumonji Domain-Containing Histone Demethylases ; genetics ; Leukemia ; genetics ; Methylation ; Nuclear Proteins ; genetics ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Proto-Oncogene Proteins c-myc ; metabolism ; RNA Interference ; RNA, Messenger ; genetics ; RNA, Small Interfering ; Repressor Proteins ; genetics
2.Experimental study on guiding bone regeneration with bovine pericardium membrane.
West China Journal of Stomatology 2004;22(5):429-431
OBJECTIVETo explore the feasibility of bovine pericardium used as a material for guiding bone regeneration.
METHODS1 cm x 1 cm x 0.5 cm defects were created on both buccal sides of the mandibles of 11 dogs. One side was covered with Glutaraldehyde (GA) cross-linking bovine pericardium; no membrane covered side was used as control. The animals were sacrificed in 2 weeks, 4 weeks, 8 weeks and 16 weeks after operation to observe the repair of the bone defects.
RESULTS(1) Wounds healed well in 10 dogs. Meanwhile, wounds in dogs of 16 weeks group healed badly and severe inflammatory response was found in bovine pericardium treated area. (2) The pericardium can be maintained in vivo for 16 weeks without absorption, there were only mild inflammatory cells invading. (3) The bone defects covered with bovine pericardium repaired better than control groups significantly.
CONCLUSION(1) GA bovine pericardium have the effect on guiding bone regeneration in the repair of dog experimental bone defects and it is possible that the bovine pericardium will be used as a new kind of GBR material; (2) GA bovine pericardium has good biocompatibility.
Animals ; Biocompatible Materials ; Bone Regeneration ; physiology ; Cattle ; Dogs ; Feasibility Studies ; Male ; Mandibular Injuries ; surgery ; Pericardium ; physiology
3.Medical institution sewage treatment in Qingpu District of Shanghai
Yi HAN ; Li-qun XIA ; Dan-dan XIANG
Shanghai Journal of Preventive Medicine 2020;32(5):445-
Objective To investigate the status of medical institution sewage treatment in Qingpu District of Shanghai, and to make corresponding measures in improving sewage management in medical institutions. Methods Questionnaire survey was conducted on selected 95 medical institutions in Qingpu District to investigate sewage treatment status and sewage samples were collected from 30 medical institutions with 20 beds or above to test their sanitary quality. Results Operating rate of sewage handling equipment was 98.9%, the proportion of medical institutions using chlorine disinfectant was 87.4%, the total qualified rate of residual chlorine was 66.7%, the total qualified rate of fecal coliform number was 70.0%, and overall qualified rate was 60.0%. Conclusion The qualified rate of medical institutions sewage in Qingpu District is not optimistic enough.It is necessary to strengthen sewage personnel training and health supervision to ensure the safety of medical institution sewage.
4. Effect and mechanism of p-hydroxybenzaldehyde on experimental intestinal fibrosis
Xi LU ; Yi-Han MA ; Bing QIN ; Yu-Hui WANG ; Xiao-Tian XU ; Xiao-Qun DUAN ; Xiao-Qun DUAN
Chinese Pharmacological Bulletin 2023;39(4):685-692
Aim To investigate the effect of p-hydroxybenzaldehyde ( HD) on intestinal fibrosis in mice based on mouse intestinal fibrosis model and in vitro EMT model,and to explore the underlying mechanism Methods HE staining, Masson staining, immunohisto-chemistry ,qPCR, Western blot and other experimental methods were used to verify the effect of HD on intestinal fibrosis in mice and the potential mechanism. Results In vivo experiments showed that compared with the normal group, the DSS-induced intestinal fibrosis model group had shortened colon, increased colon his-topathological score, increased collagen volume fraction, and significantly increased collagen I expression. After treatment with 4, 10, and 25 mg • kg
5.Small incision decompression in surgical treatment of leg fracture.
Xin-Gong LIU ; Yi-Ting HAN ; Qun-Li DUAN-MU ; Hong ZHU ; Dong-Hui HUANG ; Qi-Hui ZHAO
China Journal of Orthopaedics and Traumatology 2008;21(5):372-373
Adult
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Aged
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Aged, 80 and over
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Decompression, Surgical
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Female
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Fractures, Bone
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complications
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surgery
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Humans
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Leg Injuries
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complications
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surgery
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Male
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Middle Aged
6.The study on pathogenesis of latex allergy..
Ning LI ; Bo CEHN ; Yi-ping XU ; Jian-hua WANG ; Zu-qun WU ; Han-bing ZOU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(1):50-52
OBJECTIVEIn order to study the pathogenesis of latex allergy and the significance in the prevention and cure of occupational diseases.
METHODS651 cases in the out-patient department were tested with skin prick test (SPT), and the specific IgE (sIgE) to latex were detected by means of disk ELISA and Western-blot.
RESULTSIt was found that the positive rate of latex SPT (37.5%) and sIgE (31.25%) were rather higher in patients in comparison with those of the normal. The positive rate of latex sIgE was much higher in the high-risk group than that of the low-risk group and the normal. The serum of the patients can react with multi-bands in the latex glove extracts.
CONCLUSIONThe prevalence of latex allergy is rather high, this disease is mediated by IgE. The people in high-risk should be tested by latex allergy in order to take proper occupational and daily protection.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Case-Control Studies ; Child ; Child, Preschool ; Female ; Humans ; Immunoglobulin E ; blood ; Infant ; Latex Hypersensitivity ; diagnosis ; etiology ; prevention & control ; Male ; Middle Aged ; Occupational Diseases ; diagnosis ; etiology ; prevention & control ; Outpatients ; Skin Tests ; Young Adult
7.Study on the prevalence and genotype of hepatitis E virus among commercial swine population in Eastern and Southern China
Yu-Gang XIA ; Yi-Han LU ; An-Qun HU ; Xue QIN ; Xiao-Lian DONG ; Jian-Fu ZHU ; Qing-Wu JIANG ; Ying-Jie ZHENG
Chinese Journal of Epidemiology 2010;31(7):791-794
Objective To determine the prevalence and genotype of hepatitis E virus (HEV)among commercial swine population in Eastern and Southern China. Methods Six hundred specimens of swine bile collected from 5 slaughterhouses in Eastern and Southern China from 2007 to 2009 were tested for HEV RNA using nested RT-PCR. PCR products were sequenced for phylogenetic analysis. Results Forty-seven out of the 600 samples (7.83%) were positive for HEV RNA. Based on the 150 nt fragment within HEV ORF2, data from phylogenetic analysis revealed that all the 47 HEV isolates were identified to be genotype Ⅳ, sharing 75.0%-83.4%, 75.0%-84.6%, 71.9%-80.7% and 88.1%-91.5% nucleotide identities with prototype Ⅰ,Ⅱ,Ⅲ and Ⅳ HEV strains respectively while majority of the isolates clustered within their respective isolation sites. Conclusion HEV was widespread in commercial swine population in Eastern and Southern China that raised a serious concern about the safety regarding the consumption of pork products.
8.Seasonal pattern and phylogenetic analysis with human isolates of genotype-IV hepatitis E virus in swine herds, eastern China.
Yi-Han LU ; Ying-Jie ZHENG ; An-Qun HU ; Jian-Fu ZHU ; Fa-Di WANG ; Xue-Cai WANG ; Qing-Wu JIANG
Chinese Journal of Preventive Medicine 2009;43(6):504-508
OBJECTIVETo determine the seasonal prevalence of genotype-IV hepatitis E virus (HEV) in swine herds in Eastern China and explore the phylogenetic relationship between swine HEV and human HEV in the situation that zoonotic features of HEV had been confirmed.
METHODSFrom September 2007 to June 2008, a total of 1200 swine bile specimens were collected from three slaughter houses located in Zhejiang, Anhui and Jiangsu, the Eastern China, and detected for HEV RNA by using nested RT-PCR. The positive PCR products were sequenced. Then the swine HEV were phylogenetically determined with human HEV isolated in Eastern China.
RESULTSThe positive rate for HEV RNA in swine herds was 4.5% totally. Significant differences of HEV detection were not observed among seasonal pattern (Sep - Oct: 6%, Dec - Jan: 4.33%, Mar - Apr: 4.33%, May - Jun: 3.33%) but in geographic distribution (Jiangsu: 6%, Anhui: 5%, Zhejiang: 2.5%). Regardless of isolation from different areas,swine and human genotype-IV HEV shared a high similarity. Phylogenetically, there were 80% - 100% and 96% - 100% identities within swine genotype-lV HEV at the nucleotide and amino acid levels respectively. Between swine HEV and human HEV, there were also similarities of 76% -99% and 95% - 100%. It was noted that some human and swine isolates were clustered with bootstrap values of > 90%.
CONCLUSIONGenotype-IV HEV is widely prevalent in swine herds in Eastern China and original common ancestor of evolution and transmission was implied. The sustaining prevalence within swine herds should have a probable influence on the epidemic situation of hepatitis E in human beings.
Animals ; China ; epidemiology ; Genotype ; Geography ; Hepatitis E ; epidemiology ; Hepatitis E virus ; classification ; genetics ; isolation & purification ; Humans ; Phylogeny ; Prevalence ; Seasons ; Sequence Homology, Nucleic Acid ; Swine ; Swine Diseases ; epidemiology ; genetics ; virology
9.Establishment and application of median serum markers for second trimester screening in Qingdao region.
Dong-yi YU ; Fang WANG ; Qi LIU ; Nan JIANG ; Wei ZHAO ; Hui-ying REN ; Mei-yan HAN ; Kai ZHANG ; Shuo LI ; Qi-qi OUYANG ; Qun NIE
Chinese Journal of Medical Genetics 2012;29(5):587-591
OBJECTIVETo establish the median of serum markers for second trimester screening in Qingdao region and to assess the influence of median correction on the performance of screening.
METHODSMaternal serum alpha-fetoproteins (AFP), human chorionic gonadotrophin, free beta subunit (β -HCG) and unconjugated oestriol (uE3) were assayed for prenatal screening of 18 188 singleton pregnancies at 15-20(+ 6) weeks gestation from January 2009 to July 2010. The median of serum markers was calculated based on above results and applied for risk estimation in screening for fetal aneuploidy from August 2010 to March 2011. The screening performance, specified in terms of detection rates (DRs), false positive rates (FPRs) and odds of being affected given a positive result (OAPR) were compared between the two groups. The risks of 45 affected pregnancies detected during the study were estimated with both Caucasian and corrected medians.
RESULTSThe average level of AFP in local pregnancies was similar to that of the Caucasian population, whilst β -HCG and uE3 were respectively 11% and 33% higher than those of Caucasians. The multiple of median (MoM) value was between 0.94 and 1.02 for the dataset based on the corrected median. At a cut-off of l in 270, FPR has decreased from 5.2% to 4.9%, and DR of Down syndrome has increased from 60% to 69.2%, and OAPR has increased from 1:79 to 1:59 when evaluating risk based on the corrected median. For the 45 affected pregnancies, three Down syndrome pregnancies could be missed because their risk estimates were lower than the cut-off level based on Caucasian median.
CONCLUSIONIt is useful to establish and apply population and laboratory-specific medians in order to improve the performance of prenatal screening and diagnosis.
Adult ; Biomarkers ; blood ; Estriol ; blood ; Female ; Humans ; Lindane ; blood ; Pregnancy ; Pregnancy Trimester, Second ; Prenatal Diagnosis ; methods ; alpha-Fetoproteins ; analysis
10.Research progress in Laggera medicinal plants.
Chang-Xin ZHOU ; Di-Yao WU ; Xiang-Ping LI ; Yi-Hang WU ; Jun ZHAO ; Nan DONG ; Rong-Min YU ; Wei WEI ; Qun-Xiong ZHENG ; Han-Dong SUN ; Xiao-Jiang HAO ; Yu ZHAO
China Journal of Chinese Materia Medica 2006;31(14):1133-1140
This paper reviewed the worldwide research progresses of the genus Laggera both on phytochemical and pharmacological work in the past few decades. The main secondary metabolites of this genus are proved to be sesquitepenoids, flavonoids and phenolic acids. Phamacological investigations revealed that the certain extracts of some Laggera species possess significant bioactivities on anti-inflammation, anti-tumor and anti-viral infection. This review afforded the comprehensive description of the active components as to provide useful references to elucidate their historical clinical application on upper respiratory infection, influenza, parotitis, and recurrent herpes viral infection.
Animals
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Anti-Inflammatory Agents, Non-Steroidal
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pharmacology
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therapeutic use
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Antineoplastic Agents, Phytogenic
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pharmacology
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therapeutic use
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Antiviral Agents
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pharmacology
;
therapeutic use
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Flavonoids
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chemistry
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isolation & purification
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therapeutic use
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Humans
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Influenza, Human
;
drug therapy
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Molecular Structure
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Parotitis
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drug therapy
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Phytotherapy
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Plants, Medicinal
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chemistry
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Ranunculaceae
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chemistry
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Respiratory Tract Infections
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drug therapy
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Sesquiterpenes
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chemistry
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isolation & purification
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therapeutic use